FastQCFastQC Report
Thu 26 May 2016
SRR2048272_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048272_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10673276
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT607400.5690848807807463No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT476530.446470230883189No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261450.2449575931513436No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT113510.10634972804975716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG42050.080.778991
TCGACGG64150.059.4615822
TATCTCG149950.059.144652
ATATCTC163350.054.5377731
ATCTCGA305150.050.099251
TCTCGAG316650.046.3099942
CGACGGC91350.041.750093
GACGGCG99050.038.6483544
ACGGCGC99600.038.1965075
CTCGAGG392950.037.3725433
TCGAGGG398550.036.8950924
CGGCGCG109150.034.941566
CGAGGGC469900.031.373775
AGGGCGC490550.030.0143457
GAGGGCG500600.029.5161466
GGGCGCG499600.029.4326258
GGCGCGC610600.025.1399869
TCTCGAC26550.024.3327942
CTCGACG26650.024.2377413
CGCGCCG609550.018.3030789
Produced by FastQC (version 0.11.5)