##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048272_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10673276 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.247321815719936 34.0 31.0 34.0 30.0 34.0 2 32.40541516962552 34.0 31.0 34.0 30.0 34.0 3 32.47374283209766 34.0 31.0 34.0 30.0 34.0 4 35.94392958638004 37.0 35.0 37.0 35.0 37.0 5 35.90742102050017 37.0 35.0 37.0 35.0 37.0 6 35.84403214158427 37.0 35.0 37.0 35.0 37.0 7 35.77154914760941 37.0 35.0 37.0 33.0 37.0 8 35.733558843601536 37.0 35.0 37.0 33.0 37.0 9 37.360301654337434 39.0 37.0 39.0 34.0 39.0 10-11 37.3568955773279 39.0 37.0 39.0 34.0 39.0 12-13 37.375042582989515 39.0 37.0 39.0 34.0 39.0 14-15 38.771072349295565 40.0 38.0 41.0 34.0 41.0 16-17 38.82013118558913 40.0 38.0 41.0 35.0 41.0 18-19 38.90854761930639 40.0 38.0 41.0 35.0 41.0 20-21 38.98658097101583 40.0 38.0 41.0 35.0 41.0 22-23 39.002571937613155 40.0 39.0 41.0 36.0 41.0 24-25 39.01253120410266 40.0 38.0 41.0 36.0 41.0 26-27 38.90381959578296 40.0 38.0 41.0 35.0 41.0 28-29 38.75556333406912 40.0 38.0 41.0 35.0 41.0 30-31 38.62884062025567 40.0 38.0 41.0 35.0 41.0 32-33 38.51310502042672 40.0 38.0 41.0 35.0 41.0 34-35 38.45076839575778 40.0 38.0 41.0 35.0 41.0 36-37 38.37674046843725 40.0 38.0 41.0 34.5 41.0 38-39 38.26474950146516 40.0 38.0 41.0 34.0 41.0 40-41 38.11495641075898 40.0 37.5 41.0 34.0 41.0 42-43 37.91151971522146 40.0 37.0 41.0 33.0 41.0 44-45 37.662246108879785 40.0 37.0 41.0 33.0 41.0 46-47 37.361298864566045 40.0 37.0 41.0 33.0 41.0 48-49 37.07189727877364 40.0 37.0 41.0 32.0 41.0 50-51 36.823580923045554 40.0 36.0 41.0 31.5 41.0 52-53 36.6236733688888 40.0 36.0 41.0 31.0 41.0 54-55 36.389529653313566 39.0 35.5 41.0 31.0 41.0 56-57 36.142333619031305 39.0 35.0 41.0 30.0 41.0 58-59 35.898501734612694 39.0 35.0 41.0 30.0 41.0 60-61 35.63690046992133 38.5 35.0 40.0 30.0 41.0 62-63 35.342615191437005 38.0 35.0 40.0 29.0 41.0 64-65 35.02860682137331 37.0 35.0 40.0 29.0 41.0 66-67 34.70557765019849 37.0 34.0 40.0 28.5 41.0 68-69 34.355174456277524 36.0 34.0 39.0 28.0 41.0 70-71 34.01533062576101 36.0 34.0 39.0 28.0 41.0 72-73 33.65262436762621 35.0 34.0 38.5 27.5 40.0 74-75 33.30613534213863 35.0 34.0 37.0 27.0 40.0 76-77 32.99044904301172 35.0 33.5 37.0 26.5 39.0 78-79 32.690153191953435 35.0 33.0 36.5 26.0 39.0 80-81 32.43045912051745 35.0 33.0 36.0 26.0 38.5 82-83 32.19010634598037 35.0 33.0 36.0 26.0 37.0 84-85 31.951444476841036 35.0 33.0 35.0 25.5 37.0 86-87 31.783587438383492 35.0 33.0 35.0 25.0 36.5 88-89 31.64066023402749 35.0 33.0 35.0 25.0 36.0 90-91 31.492046818615016 35.0 33.0 35.0 25.0 36.0 92-93 31.29625932094326 35.0 33.0 35.0 24.5 36.0 94-95 31.158494214897093 35.0 33.0 35.0 24.0 35.0 96-97 31.03242907800754 35.0 33.0 35.0 23.5 35.0 98-99 30.882284689349362 35.0 33.0 35.0 21.5 35.0 100-101 30.012063634445504 34.5 31.5 35.0 10.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5119116195910394 1101 2 0.4585826493346019 1101 3 0.4235337887271484 1101 4 0.33877454543262076 1101 5 0.31521913517217115 1101 6 0.2892004948250744 1101 7 0.29082652880491366 1101 8 0.2781779232419339 1101 9 0.33770863649804284 1101 10-11 0.30207320974594154 1101 12-13 0.3633797336664344 1101 14-15 0.4620532467308536 1101 16-17 0.45032069294797594 1101 18-19 0.5096917848900446 1101 20-21 0.48261469772104704 1101 22-23 0.48482536224478423 1101 24-25 0.4843494661768517 1101 26-27 0.5291148608110205 1101 28-29 0.5397629981710352 1101 30-31 0.5293626384530938 1101 32-33 0.516739229913469 1101 34-35 0.5015820246936684 1101 36-37 0.5462064825459407 1101 38-39 0.5435170414146668 1101 40-41 0.513803113126734 1101 42-43 0.503345676761974 1101 44-45 0.4614095998891514 1101 46-47 0.40908689428221123 1101 48-49 0.42403484873724295 1101 50-51 0.44458636431866694 1101 52-53 0.4276814480957327 1101 54-55 0.4668396154665899 1101 56-57 0.45865100748006427 1101 58-59 0.46592663313460747 1101 60-61 0.4883743696521208 1101 62-63 0.5065799809398897 1101 64-65 0.4530424444970933 1101 66-67 0.4309731735905018 1101 68-69 0.44985018782804076 1101 70-71 0.45704089671367853 1101 72-73 0.4491712838779023 1101 74-75 0.40592444402882677 1101 76-77 0.38670176896194874 1101 78-79 0.37833293868126106 1101 80-81 0.3571604707740761 1101 82-83 0.3800671013713526 1101 84-85 0.41276893793702385 1101 86-87 0.41907495951981844 1101 88-89 0.4418170449742078 1101 90-91 0.4272389148628868 1101 92-93 0.4529558356023138 1101 94-95 0.4463666625203402 1101 96-97 0.48549000888242944 1101 98-99 0.47541148343531603 1101 100-101 0.6055238078233316 1102 1 0.44125392231170935 1102 2 0.4176019128820485 1102 3 0.37046559492848274 1102 4 0.26017821395181073 1102 5 0.2691828768894382 1102 6 0.2518279927514513 1102 7 0.23556451254152222 1102 8 0.205812625779636 1102 9 0.25520761923736757 1102 10-11 0.25093471176464277 1102 12-13 0.2730065321497861 1102 14-15 0.3812921933772415 1102 16-17 0.42011366279990625 1102 18-19 0.44118341872317757 1102 20-21 0.442970665950817 1102 22-23 0.4762909907850954 1102 24-25 0.47101325023690066 1102 26-27 0.4746076257247438 1102 28-29 0.43844753762227384 1102 30-31 0.44888678960615636 1102 32-33 0.42168071358796055 1102 34-35 0.48106115813254746 1102 36-37 0.5054003756252641 1102 38-39 0.4705568614477329 1102 40-41 0.47987656955740476 1102 42-43 0.4583539533226144 1102 44-45 0.4258377881966027 1102 46-47 0.33525530214339483 1102 48-49 0.32671686799044863 1102 50-51 0.3799495226107581 1102 52-53 0.399150199993926 1102 54-55 0.3745414881251392 1102 56-57 0.3852655603324848 1102 58-59 0.3760895335769163 1102 60-61 0.3717080338019372 1102 62-63 0.36485244967033026 1102 64-65 0.36185288836984597 1102 66-67 0.29608902023846184 1102 68-69 0.2946720075615019 1102 70-71 0.304336157642922 1102 72-73 0.2937803245310704 1102 74-75 0.3021374752224446 1102 76-77 0.28315882580483276 1102 78-79 0.31909937880099193 1102 80-81 0.3050920242905022 1102 82-83 0.3301297322051653 1102 84-85 0.3121913219741117 1102 86-87 0.28315704277821396 1102 88-89 0.3308739000121079 1102 90-91 0.37610488810011233 1102 92-93 0.39854547605139246 1102 94-95 0.33852372954232024 1102 96-97 0.3107413259173839 1102 98-99 0.34787976960972244 1102 100-101 0.5087625964052549 1103 1 0.4135497973820179 1103 2 0.33636199394209854 1103 3 0.33225786286566006 1103 4 0.2575413124213455 1103 5 0.26013936982706554 1103 6 0.2368838685273147 1103 7 0.21513445427471112 1103 8 0.20277596060900294 1103 9 0.2582311778050226 1103 10-11 0.2769684552652336 1103 12-13 0.2664593919860607 1103 14-15 0.344416888373118 1103 16-17 0.3701727772541119 1103 18-19 0.3692728165347887 1103 20-21 0.36139895396878785 1103 22-23 0.3829766689672809 1103 24-25 0.3985990850123642 1103 26-27 0.39232851240726063 1103 28-29 0.40944324992572234 1103 30-31 0.39852564757271836 1103 32-33 0.4176526135211276 1103 34-35 0.4630795357947832 1103 36-37 0.4467319746406204 1103 38-39 0.43024355974009865 1103 40-41 0.4636376232008885 1103 42-43 0.45211882380487367 1103 44-45 0.4346393498177292 1103 46-47 0.4048945300346318 1103 48-49 0.3784616018308711 1103 50-51 0.3729816972387283 1103 52-53 0.36987269474638396 1103 54-55 0.36505810141812844 1103 56-57 0.3911913372723106 1103 58-59 0.3676964937465641 1103 60-61 0.3994798720973236 1103 62-63 0.3867292319233542 1103 64-65 0.3538658225046376 1103 66-67 0.348608146079485 1103 68-69 0.36302226841964824 1103 70-71 0.3536908473079663 1103 72-73 0.3076045936480938 1103 74-75 0.2926696816982357 1103 76-77 0.28386462250010425 1103 78-79 0.3462998671995692 1103 80-81 0.3416538487492389 1103 82-83 0.3746783530475568 1103 84-85 0.35647761477022755 1103 86-87 0.38572952596747 1103 88-89 0.4387972824858082 1103 90-91 0.4033152493229899 1103 92-93 0.4273410257839707 1103 94-95 0.40105682677714327 1103 96-97 0.4528820800541702 1103 98-99 0.4543775211536065 1103 100-101 0.5728315180282593 1104 1 0.3285121614161568 1104 2 0.3296480183817394 1104 3 0.31107825831033153 1104 4 0.22924734022107884 1104 5 0.20658334961164115 1104 6 0.17948317010348092 1104 7 0.17571101646584708 1104 8 0.16138362087060898 1104 9 0.21143021172375143 1104 10-11 0.1967311410853938 1104 12-13 0.23961679241560319 1104 14-15 0.3096968591818765 1104 16-17 0.3701157975960072 1104 18-19 0.307687622604611 1104 20-21 0.3077393889519868 1104 22-23 0.3582036766483796 1104 24-25 0.34535092230616726 1104 26-27 0.34032380290477704 1104 28-29 0.3473480252392278 1104 30-31 0.3027829496473302 1104 32-33 0.2765749414064942 1104 34-35 0.34904985611957073 1104 36-37 0.3687060191569813 1104 38-39 0.2850433901223539 1104 40-41 0.2864547728772848 1104 42-43 0.2954677085222883 1104 44-45 0.3226307767689889 1104 46-47 0.3025341786419915 1104 48-49 0.27573362710423055 1104 50-51 0.2839508377308846 1104 52-53 0.2529496667497213 1104 54-55 0.24076911863506467 1104 56-57 0.2670493607091231 1104 58-59 0.31402185746880207 1104 60-61 0.2866095344528645 1104 62-63 0.24409627846058157 1104 64-65 0.26710288180353103 1104 66-67 0.2628075014504532 1104 68-69 0.2714737248662473 1104 70-71 0.21912184828446613 1104 72-73 0.22309419916027196 1104 74-75 0.20186449992890942 1104 76-77 0.18041616408009276 1104 78-79 0.23755401121685082 1104 80-81 0.25283684259983374 1104 82-83 0.24535965331676834 1104 84-85 0.20635033887538867 1104 86-87 0.21515871737275916 1104 88-89 0.19229887509761667 1104 90-91 0.19597105769702594 1104 92-93 0.25446008401698705 1104 94-95 0.30702978399312286 1104 96-97 0.2888903984074176 1104 98-99 0.30192231260740954 1104 100-101 0.40791745527618417 1105 1 0.26498401874037114 1105 2 0.22189156645433172 1105 3 0.19715990329964939 1105 4 0.1566740334023109 1105 5 0.16096629771924142 1105 6 0.12084425056602299 1105 7 0.12880918546235165 1105 8 0.13303556787004567 1105 9 0.1585868846973213 1105 10-11 0.17817289812245463 1105 12-13 0.2141008438520302 1105 14-15 0.2555542835097242 1105 16-17 0.2606797918651793 1105 18-19 0.26857737882414057 1105 20-21 0.2891778563640912 1105 22-23 0.27171952645754516 1105 24-25 0.24528764681894444 1105 26-27 0.2634311835077483 1105 28-29 0.3181505705028158 1105 30-31 0.26408428325428446 1105 32-33 0.2759606110604338 1105 34-35 0.24628270371852068 1105 36-37 0.2806068469671388 1105 38-39 0.29149345165672713 1105 40-41 0.2871994275267511 1105 42-43 0.27256505727937963 1105 44-45 0.2402647292354132 1105 46-47 0.27416489519431764 1105 48-49 0.23226220736798098 1105 50-51 0.2506758217363654 1105 52-53 0.2501237454068601 1105 54-55 0.23605832974426022 1105 56-57 0.278133672089389 1105 58-59 0.2516803501433884 1105 60-61 0.2870301533843076 1105 62-63 0.2569746217170419 1105 64-65 0.27676583007046673 1105 66-67 0.20176975369359695 1105 68-69 0.22350641213811429 1105 70-71 0.1835397152246756 1105 72-73 0.1741338180417955 1105 74-75 0.10168462332420347 1105 76-77 0.0983596389557988 1105 78-79 0.1607416976275644 1105 80-81 0.20358793095024907 1105 82-83 0.19641717362374322 1105 84-85 0.16324535665631856 1105 86-87 0.16209613892270625 1105 88-89 0.1335383620045505 1105 90-91 0.144776414966163 1105 92-93 0.1448989674801986 1105 94-95 0.1506171526124973 1105 96-97 0.14948482816406283 1105 98-99 0.1785533291218755 1105 100-101 0.3027463618387749 1106 1 0.26709380170474617 1106 2 0.267510584859771 1106 3 0.22236621608998774 1106 4 0.15940805571302974 1106 5 0.17233260862552413 1106 6 0.11232759605810827 1106 7 0.11915574940743312 1106 8 0.11756250824487324 1106 9 0.13919395571676318 1106 10-11 0.16705867688290255 1106 12-13 0.1865226133774769 1106 14-15 0.17981357995353875 1106 16-17 0.1947053478427776 1106 18-19 0.23052481183035667 1106 20-21 0.1965815097919119 1106 22-23 0.22185264169599606 1106 24-25 0.22760807331430755 1106 26-27 0.2354239266621363 1106 28-29 0.21398537783850458 1106 30-31 0.18449102494560776 1106 32-33 0.1382531262253366 1106 34-35 0.1980277041417864 1106 36-37 0.23530649308745666 1106 38-39 0.2384652463721224 1106 40-41 0.21150284948370057 1106 42-43 0.17278760943293747 1106 44-45 0.14878558816330667 1106 46-47 0.1809389973296689 1106 48-49 0.16543889680141177 1106 50-51 0.17224765376076334 1106 52-53 0.1626777067447165 1106 54-55 0.16627322889219442 1106 56-57 0.23596082575076593 1106 58-59 0.20772030141140618 1106 60-61 0.17915081121053333 1106 62-63 0.24625727724541946 1106 64-65 0.21374512482749708 1106 66-67 0.20031618414625996 1106 68-69 0.18686296318970363 1106 70-71 0.17387233687842496 1106 72-73 0.16352099687200905 1106 74-75 0.13807957684164762 1106 76-77 0.08671436311168179 1106 78-79 0.12048114980459701 1106 80-81 0.09192383552044703 1106 82-83 0.10252632542359663 1106 84-85 0.12481109018206382 1106 86-87 0.18198857473022656 1106 88-89 0.17034295182691395 1106 90-91 0.16700983295419647 1106 92-93 0.23362190019436824 1106 94-95 0.2511713482443625 1106 96-97 0.2763057677888199 1106 98-99 0.24053841801683262 1106 100-101 0.39657891237054343 1107 1 0.2180704405290257 1107 2 0.19588015753668486 1107 3 0.17647020335360963 1107 4 0.14933224773646003 1107 5 0.16607178630805208 1107 6 0.14137692228820242 1107 7 0.06679130168095782 1107 8 0.08333330113187287 1107 9 0.048657049664548424 1107 10-11 0.11186339732752515 1107 12-13 0.07706772921915928 1107 14-15 0.0723886406832932 1107 16-17 0.1008796766960387 1107 18-19 0.08140284810542653 1107 20-21 0.106488698051038 1107 22-23 0.1579482501534173 1107 24-25 0.15816234328394785 1107 26-27 0.11598789524317255 1107 28-29 0.15149257381114722 1107 30-31 0.12354970097738516 1107 32-33 0.19296129160537845 1107 34-35 0.20842583497547906 1107 36-37 0.20215455498161106 1107 38-39 0.14456589980897405 1107 40-41 0.1457554665490548 1107 42-43 0.14436031300310503 1107 44-45 0.14527732817927586 1107 46-47 0.17580501469981868 1107 48-49 0.09890232481247807 1107 50-51 0.1287362232949789 1107 52-53 0.1152856973795977 1107 54-55 0.039069205272959096 1107 56-57 0.08467351941813916 1107 58-59 0.09898569512114364 1107 60-61 0.0976853396850359 1107 62-63 0.1992459950946639 1107 64-65 0.18016959561511214 1107 66-67 0.14508958261342286 1107 68-69 0.13083580083892343 1107 70-71 0.12298901174070664 1107 72-73 0.10767980964854473 1107 74-75 0.07313316592339447 1107 76-77 0.09937771523536298 1107 78-79 0.12224331943500744 1107 80-81 0.11387365059458432 1107 82-83 0.13212054629897807 1107 84-85 0.09498074214938157 1107 86-87 0.1532541556061524 1107 88-89 0.13663313087689488 1107 90-91 0.12642511444930804 1107 92-93 0.14417081324092962 1107 94-95 0.18763371734856094 1107 96-97 0.19336142817634538 1107 98-99 0.183654530813385 1107 100-101 0.26283988215227794 1108 1 0.15730674462822947 1108 2 0.13890931755408786 1108 3 0.1258519872450421 1108 4 0.09628293112628228 1108 5 0.1047396613026379 1108 6 0.07017781697840775 1108 7 0.07642909847964319 1108 8 0.12390677330987643 1108 9 0.13794051874636182 1108 10-11 0.05596902270944781 1108 12-13 0.10061193430079385 1108 14-15 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-0.4208273369540123 2214 100-101 -0.4629015448450815 2215 1 -0.5034026128024358 2215 2 -0.4760488697281069 2215 3 -0.43294090950188746 2215 4 -0.33763290213862973 2215 5 -0.3501403753838943 2215 6 -0.35845277032302647 2215 7 -0.31927636038878404 2215 8 -0.3157973967060954 2215 9 -0.3634285777689925 2215 10-11 -0.38769100325932015 2215 12-13 -0.4395262010438614 2215 14-15 -0.5270800094447381 2215 16-17 -0.5094227267956768 2215 18-19 -0.5732812477551406 2215 20-21 -0.5044170342845078 2215 22-23 -0.5138655327179791 2215 24-25 -0.5433618619405891 2215 26-27 -0.49613613168651227 2215 28-29 -0.46560339600909373 2215 30-31 -0.5214167057754153 2215 32-33 -0.5212799407774469 2215 34-35 -0.5625171997277505 2215 36-37 -0.555056953023751 2215 38-39 -0.5630208582636484 2215 40-41 -0.5521570285046877 2215 42-43 -0.5108077817667578 2215 44-45 -0.5202920723111788 2215 46-47 -0.5264230512233681 2215 48-49 -0.5351507752869509 2215 50-51 -0.49581737395511993 2215 52-53 -0.4944708961788322 2215 54-55 -0.44073663053235634 2215 56-57 -0.5061984176884025 2215 58-59 -0.4675593463631671 2215 60-61 -0.5411194513234676 2215 62-63 -0.452953931090164 2215 64-65 -0.49272800328023436 2215 66-67 -0.49562928441552856 2215 68-69 -0.39329710043696053 2215 70-71 -0.3863759436809957 2215 72-73 -0.3992983634627194 2215 74-75 -0.3712046413070027 2215 76-77 -0.3502705150826557 2215 78-79 -0.34508598527934 2215 80-81 -0.38294189961763436 2215 82-83 -0.3467559895661054 2215 84-85 -0.33410238897261735 2215 86-87 -0.4022646071267353 2215 88-89 -0.37598081670148176 2215 90-91 -0.40810734815881133 2215 92-93 -0.3803714913898695 2215 94-95 -0.4039558289911156 2215 96-97 -0.4641550494078004 2215 98-99 -0.5332453789374725 2215 100-101 -0.6045037449085093 2216 1 -0.6107087904498734 2216 2 -0.61274250405733 2216 3 -0.5630807857171725 2216 4 -0.44410375079492326 2216 5 -0.45320431769805225 2216 6 -0.41976793929698175 2216 7 -0.3671384882488056 2216 8 -0.3513813041913423 2216 9 -0.44001980708914346 2216 10-11 -0.44620624396207376 2216 12-13 -0.4731929357007516 2216 14-15 -0.6162643177244078 2216 16-17 -0.6197146600751822 2216 18-19 -0.6886662281769631 2216 20-21 -0.6566336824858467 2216 22-23 -0.7044079148211395 2216 24-25 -0.6439861714778772 2216 26-27 -0.6307694545359155 2216 28-29 -0.6998550916537667 2216 30-31 -0.659475772395389 2216 32-33 -0.7186129603669542 2216 34-35 -0.7735011540681356 2216 36-37 -0.7688419374958784 2216 38-39 -0.7239101630416656 2216 40-41 -0.7843572198124491 2216 42-43 -0.661000397311085 2216 44-45 -0.6277374456524427 2216 46-47 -0.6178177588390668 2216 48-49 -0.5916108525468289 2216 50-51 -0.48585945253829266 2216 52-53 -0.5637938792071182 2216 54-55 -0.5665034519250156 2216 56-57 -0.5409958135073296 2216 58-59 -0.5571460324362008 2216 60-61 -0.5562205996954219 2216 62-63 -0.644377778401342 2216 64-65 -0.5592997334363119 2216 66-67 -0.5464589214067175 2216 68-69 -0.5088492359412626 2216 70-71 -0.538799819078001 2216 72-73 -0.5563213350822309 2216 74-75 -0.5544988815573362 2216 76-77 -0.6209393441209556 2216 78-79 -0.6165032072862928 2216 80-81 -0.6024244836615438 2216 82-83 -0.5783700194516008 2216 84-85 -0.550442366117295 2216 86-87 -0.5471541382016198 2216 88-89 -0.5739272526337018 2216 90-91 -0.6465252533399521 2216 92-93 -0.5699134254099683 2216 94-95 -0.6074000019598316 2216 96-97 -0.6062070934757458 2216 98-99 -0.7200244686156978 2216 100-101 -0.8884052971425476 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 337.0 3 109.0 4 112.0 5 204.0 6 458.0 7 1249.0 8 2982.0 9 5122.0 10 8104.0 11 9446.0 12 10999.0 13 16113.0 14 27348.0 15 48609.0 16 67085.0 17 92154.0 18 91220.0 19 60115.0 20 47659.0 21 42109.0 22 38849.0 23 38444.0 24 40798.0 25 46645.0 26 56673.0 27 70997.0 28 91089.0 29 115158.0 30 149001.0 31 198134.0 32 270901.0 33 376144.0 34 527818.0 35 768971.0 36 1341292.0 37 2502859.0 38 2819530.0 39 687816.0 40 623.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 24.025563368568633 37.36258655733669 17.433270154739006 21.178579919355673 2 23.284837097722626 28.89163666009919 29.64830350637576 18.175222735802425 3 22.360205616556883 28.165205305030845 24.455253383102544 25.01933569530973 4 23.619065036826555 26.562687969466918 26.7458369857577 23.072410007948825 5 23.292820311214662 27.274756129233424 25.292984084736496 24.139439474815415 6 24.700120188028492 25.910498332470745 26.16358838654599 23.225793092954778 7 23.792807381726096 27.80829428565325 24.670551009830533 23.728347322790118 8 25.266211388890163 25.91550174638494 26.065194560839394 22.753092303885502 9 23.429704244507494 26.217442517180288 26.171542832772243 24.181310405539968 10-11 24.346315976463085 26.13118034238035 25.65713188715442 23.86537179400214 12-13 23.924285289727354 26.276819788038836 25.63551715518272 24.16337776705109 14-15 23.350563133337328 26.335772494871883 26.16063875073355 24.153025621057235 16-17 23.404234088952634 26.398928501427303 26.203107649422723 23.99372976019734 18-19 22.818504833942267 26.536510439718793 27.255867832894044 23.3891168934449 20-21 21.882334908232487 26.357680622144503 28.312178941123605 23.447805528499405 22-23 21.535916266274896 26.110520309419766 29.164635037590603 23.18892838671474 24-25 21.51359664656027 25.733377556163934 30.355323885387914 22.397701911887886 26-27 21.48336954861609 25.62309963466135 30.979953693162386 21.913577123560174 28-29 21.570872189635434 25.748785561937982 30.929143271363774 21.75119897706281 30-31 21.579809058163345 25.63149163430732 30.843163647077436 21.945535660451903 32-33 21.551867180263002 25.60649149137848 30.918246608127077 21.923394720231443 34-35 21.646742111268328 25.78428694058105 30.80486078201772 21.7641101661329 36-37 21.604130727997664 25.639883199872276 30.721331482480167 22.03465458964989 38-39 21.648259634623894 25.656387036182704 30.715396097692967 21.979957231500432 40-41 21.816848922486404 25.880367002596017 30.28126978071213 22.021514294205453 42-43 22.03571330864113 25.87638977948289 29.883435039063922 22.204461872812058 44-45 21.96452148337586 26.00851884650973 29.67863849862029 22.348321171494113 46-47 22.291520429154087 26.325876891031395 28.866132572604702 22.51647010720982 48-49 22.424645953012227 26.496651844742363 28.254300429423402 22.82440177282201 50-51 22.59566322467441 26.433388399213136 28.02322361007061 22.94772476604184 52-53 22.618999266425472 26.636602244309877 27.637920467291522 23.106478021973132 54-55 22.709734585162156 26.497784614153613 27.485377432142492 23.30710336854174 56-57 22.67361032867303 26.435395642051134 27.599805615442484 23.291188413833346 58-59 22.815006680186013 26.691477536837997 27.31675587792186 23.176759905054126 60-61 22.837887501730403 26.60548167952181 27.190260371930453 23.366370446817335 62-63 22.791973047856136 26.6209012912492 27.31259609084477 23.274529570049893 64-65 22.94367104808012 26.827701693614365 27.09566554498585 23.13296171331967 66-67 22.90221627067177 26.75606640824737 27.00482914133946 23.336888179741404 68-69 22.8934592993977 26.787785720275327 27.091371930467258 23.22738304985972 70-71 22.94544619151332 27.00436287306144 26.905011616901408 23.145179318523837 72-73 22.95741858595067 26.875435233812343 26.797149949042655 23.369996231194325 74-75 22.889430692831596 26.845079389320485 26.976741026642216 23.288748891205703 76-77 22.984747291640822 27.006984375521164 26.78837715065661 23.219891182181403 78-79 23.0043477757779 26.916582378121717 26.72164476897818 23.357425077122205 80-81 22.908269592948187 26.88294408576017 26.87900896392646 23.329777357365185 82-83 22.94601557806984 27.04334335665623 26.72354924662991 23.28709181864402 84-85 22.922697327275245 26.920180296041625 26.69520844914466 23.461913927538472 86-87 22.874739553540167 26.83612587407739 26.8827548908465 23.40637968153595 88-89 22.969489473620104 27.00945214476923 26.61490380606238 23.406154575548292 90-91 22.962892137278875 26.961403153063678 26.5537033094951 23.522001400162353 92-93 22.88487894390051 26.97102678499276 26.730181796717385 23.413912474389345 94-95 22.97541105947183 27.22369005288032 26.499308674090592 23.301590213557258 96-97 22.91165477429479 27.24228113919416 26.327716578399894 23.518347508111162 98-99 22.882617898611173 27.214783699493665 26.51504657114191 23.38755183075325 100-101 22.950344828555455 27.36100222452657 26.249853896130514 23.438799050787463 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 906.0 1 620.5 2 282.5 3 225.5 4 211.5 5 205.0 6 192.5 7 183.5 8 198.0 9 210.5 10 220.0 11 235.0 12 312.0 13 556.5 14 837.5 15 1297.5 16 5267.0 17 6156.5 18 3689.5 19 4285.5 20 5594.5 21 8053.0 22 11860.5 23 17606.0 24 25246.0 25 34491.5 26 45974.0 27 60377.0 28 75965.0 29 92788.0 30 112029.5 31 134712.5 32 162839.0 33 190790.0 34 211599.5 35 234232.0 36 262239.5 37 295234.0 38 322265.0 39 338561.0 40 352793.0 41 371539.0 42 400321.0 43 426084.5 44 438592.5 45 441651.5 46 441316.5 47 437268.5 48 436390.0 49 429089.5 50 413359.0 51 396303.5 52 358927.5 53 312668.0 54 282508.0 55 258978.0 56 232796.5 57 208348.5 58 191556.5 59 177683.5 60 157769.5 61 138013.0 62 122552.5 63 109137.5 64 95747.5 65 81239.0 66 67383.0 67 54981.0 68 44195.5 69 34917.0 70 26593.5 71 19737.5 72 14466.0 73 10514.5 74 7373.0 75 4760.0 76 2906.5 77 1895.0 78 1396.5 79 845.5 80 346.0 81 128.5 82 53.0 83 22.0 84 13.0 85 8.5 86 4.0 87 2.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2949984615782446 2 0.01874775842018889 3 1.0306114074066856E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 7.963815420869844E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 4.216137575754623E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0037102010666640686 24-25 5.246748983161308E-4 26-27 0.009238025888209019 28-29 0.01182392360133852 30-31 0.005148372439727034 32-33 4.028753683498862E-4 34-35 1.311687245790327E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 9.837654343427453E-5 50-51 0.0 52-53 0.001831677546800064 54-55 0.0028529197595939613 56-57 0.005785477673396621 58-59 0.006267991195955207 60-61 0.0016724012383826671 62-63 0.004056861267337226 64-65 7.495355690230441E-5 66-67 5.012519117841607E-4 68-69 2.9044503299642955E-4 70-71 3.1855261683479374E-4 72-73 0.001250787480807205 74-75 7.823277501678023E-4 76-77 2.248606707069132E-4 78-79 0.0011008803670025961 80-81 0.0015271787218844525 82-83 2.3422986531970126E-5 84-85 0.0036071399259233997 86-87 0.0069050964296247935 88-89 0.005302964150838037 90-91 0.0021361763717156756 92-93 0.0021408609690220697 94-95 0.0028763427461259315 96-97 0.003073095832994481 98-99 0.0018410467414128522 100-101 0.0020471690228941893 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.0673276E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.61493788505972 #Duplication Level Percentage of deduplicated Percentage of total 1 75.83860948200726 35.35212070293069 2 13.947983927242365 13.003688087804283 3 4.2852192758901575 5.992656911084406 4 1.7186412565457159 3.2045742168231808 5 0.9408765566854721 2.192945112370107 6 0.536528833695256 1.5006154953748734 7 0.35647154438288914 1.1631829229439778 8 0.26293496824697327 0.9805357770114246 9 0.22360915210763213 0.9381174062425328 >10 1.5735757410088143 15.002020768533686 >50 0.16462527411119024 5.330729003015967 >100 0.14798080210483056 12.458466041205622 >500 0.002198233747833512 0.6519031332812537 >1k 5.838815740472036E-4 0.5529582643272154 >5k 8.053532480885047E-5 0.30626348875977183 >10k+ 8.053532480885047E-5 1.3692226682910746 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 60740 0.5690848807807463 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 47653 0.446470230883189 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26145 0.2449575931513436 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 11351 0.10634972804975716 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.6215167676728306E-5 9.369194612788051E-4 0.0 0.0 0.0 2 5.6215167676728306E-5 0.018588482111771495 0.0 0.0 0.0 3 9.369194612788052E-5 0.02331992539122946 0.0 9.369194612788051E-6 0.0 4 9.369194612788052E-5 0.0350501570464401 0.0 1.8738389225576102E-5 0.0 5 1.1243033535345661E-4 0.03930377140064587 0.0 2.8107583838364153E-5 0.0 6 1.5927630841739687E-4 0.05302964150838037 0.0 3.7476778451152204E-5 0.0 7 1.8738389225576103E-4 0.05976609243497498 0.0 4.684597306394026E-5 0.0 8 2.1549147609412517E-4 0.06310152571712753 0.0 4.684597306394026E-5 0.0 9 0.002220499123230768 0.06611840638244527 0.0 4.684597306394026E-5 0.0 10-11 0.004698651098313208 0.06809062184843717 0.0 4.684597306394026E-5 0.0 12-13 0.009275502666660171 0.07334674002621125 0.0 5.6215167676728306E-5 0.0 14-15 0.010085938000666338 0.07572651545785943 0.0 6.558436228951635E-5 0.0 16-17 0.01064808967743362 0.07885114186122424 0.0 6.558436228951635E-5 0.0 18-19 0.011481947997971757 0.08289863393394868 0.0 7.026895959591038E-5 0.0 20-21 0.012779581451842902 0.08754575446189156 0.0 8.432275151509246E-5 0.0 22-23 0.019815846606046728 0.09269412690161859 0.0 8.432275151509246E-5 0.0 24-25 0.02633212145924082 0.09914950198982955 0.0 8.432275151509246E-5 0.0 26-27 0.027325256088196352 0.11349842353931446 0.0 8.432275151509246E-5 0.0 28-29 0.02851982840132683 0.15121880105039914 0.0 8.432275151509246E-5 0.0 30-31 0.029953315177083398 0.215299407604563 0.0 8.432275151509246E-5 0.0 32-33 0.03215507591108859 0.28233131046175514 0.0 8.900734882148649E-5 0.0 34-35 0.035209433354857496 0.3131606453351342 0.0 9.369194612788052E-5 0.0 36-37 0.039828446298962004 0.3232418987384942 0.0 9.837654343427453E-5 0.0 38-39 0.04603553772993409 0.32656796282603395 0.0 1.0774573804706259E-4 0.0 40-41 0.05346530905787501 0.3279499190314201 0.0 1.1243033535345661E-4 0.0 42-43 0.06333107098514083 0.3284371171512851 0.0 1.1711493265985064E-4 0.0 44-45 0.07559066213597398 0.3288072003384903 0.0 1.264841272726387E-4 0.0 46-47 0.09067975005986914 0.32890089228461816 0.0 1.311687245790327E-4 0.0 48-49 0.10902463311170815 0.3290133226199716 0.0 1.4053791919182076E-4 0.0 50-51 0.13106566343829207 0.3291163837607123 0.0 1.4053791919182076E-4 0.0 52-53 0.1565779803689139 0.3292007065122273 0.0 1.4522251649821478E-4 0.0 54-55 0.18508843957562796 0.32927097547182327 0.0 1.5927630841739687E-4 0.0 56-57 0.21718261572173342 0.3293318752368064 0.0 1.5927630841739687E-4 0.0 58-59 0.25471092474325596 0.32937872120987033 0.0 1.6396090572379088E-4 0.0 60-61 0.2961930338913751 0.32939277500178954 0.0 1.6864550303018493E-4 0.0 62-63 0.3434418823236652 0.32940214419640235 0.0 1.82699294949367E-4 0.0 64-65 0.3948740761505652 0.32942556718293425 0.0 1.8738389225576103E-4 0.0 66-67 0.4520636400670235 0.32943493637754706 0.0 1.8738389225576103E-4 0.0 68-69 0.5148372439727034 0.3294630439613854 0.0 1.967530868685491E-4 0.0 70-71 0.582885704445383 0.3294864669479174 0.0 1.967530868685491E-4 0.0 72-73 0.6569023418864086 0.3294864669479174 0.0 1.967530868685491E-4 0.0 74-75 0.7378943447166549 0.3294864669479174 0.0 1.967530868685491E-4 0.0 76-77 0.8237723825374703 0.3294958361425302 0.0 1.967530868685491E-4 9.369194612788051E-6 78-79 0.917454959470738 0.32950520533714295 0.0 1.967530868685491E-4 9.369194612788051E-6 80-81 1.0177240802167957 0.32950520533714295 0.0 1.967530868685491E-4 9.369194612788051E-6 82-83 1.1243174073264854 0.32950520533714295 0.0 2.014376841749431E-4 9.369194612788051E-6 84-85 1.2411512641479523 0.32951457453175576 0.0 2.1549147609412517E-4 9.369194612788051E-6 86-87 1.3636909604886074 0.32951457453175576 0.0 2.1549147609412517E-4 9.369194612788051E-6 88-89 1.4950330151679765 0.3295333129209813 0.0 2.1549147609412517E-4 9.369194612788051E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 4205 0.0 80.77899 1 TCGACGG 6415 0.0 59.461582 2 TATCTCG 14995 0.0 59.14465 2 ATATCTC 16335 0.0 54.537773 1 ATCTCGA 30515 0.0 50.09925 1 TCTCGAG 31665 0.0 46.309994 2 CGACGGC 9135 0.0 41.75009 3 GACGGCG 9905 0.0 38.648354 4 ACGGCGC 9960 0.0 38.196507 5 CTCGAGG 39295 0.0 37.372543 3 TCGAGGG 39855 0.0 36.895092 4 CGGCGCG 10915 0.0 34.94156 6 CGAGGGC 46990 0.0 31.37377 5 AGGGCGC 49055 0.0 30.014345 7 GAGGGCG 50060 0.0 29.516146 6 GGGCGCG 49960 0.0 29.432625 8 GGCGCGC 61060 0.0 25.139986 9 TCTCGAC 2655 0.0 24.332794 2 CTCGACG 2665 0.0 24.237741 3 CGCGCCG 60955 0.0 18.303078 9 >>END_MODULE