FastQCFastQC Report
Thu 26 May 2016
SRR2048271_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048271_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8202091
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT590420.7198408308320403No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC126780.1545703406606925No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG126420.15413142819312783No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA113750.13868414773744892No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC95440.11636057195659008No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG90860.1107766300081284No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA88600.1080212350728613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG306300.093.4713441
TCGACGG323700.088.2240452
TATCTCG278050.086.575992
CGACGGC338750.084.2623443
ACGGCGC342000.082.7242055
GACGGCG344250.082.586334
ATCTCGA292700.082.096643
TCTCGAG277900.081.357774
ATATCTC300350.080.5618061
CGGCGCG352700.080.175936
CTCGAGG287150.078.089215
TCGAGGG290300.077.106516
AGGGCGC300250.074.575179
CGAGGGC326600.068.530237
GAGGGCG334250.066.947158
CTCGACG22750.059.526915
TCTCGAC26200.052.052764
CGCGCCG650100.045.495049
GGCGCGC650550.045.1429257
GCGCGCC656750.044.846648
Produced by FastQC (version 0.11.5)