FastQCFastQC Report
Thu 26 May 2016
SRR2048271_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048271_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8202091
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT378970.4620407162027341No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT311100.3792935240538053No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163600.19946133248216827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG28600.081.468931
TCGACGG44350.058.269562
TATCTCG106100.057.8025932
ATATCTC113950.054.192581
ATCTCGA209900.049.0786321
TCTCGAG217550.045.179132
CGACGGC64450.040.1643943
GACGGCG68450.037.9560934
ACGGCGC70000.036.979935
CTCGAGG271700.036.274053
TCGAGGG277200.035.520064
CGGCGCG78350.033.2813536
CGAGGGC332800.029.6286355
AGGGCGC343600.028.614417
GGGCGCG352400.027.926828
GAGGGCG361000.027.3667836
CTCGACG18850.026.9612523
TCTCGAC20250.026.039612
GGCGCGC434950.023.8054739
CGCGCCG430800.017.6291949
Produced by FastQC (version 0.11.5)