##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048271_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8202091 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26741437031118 34.0 31.0 34.0 30.0 34.0 2 32.42536689729485 34.0 31.0 34.0 30.0 34.0 3 32.49618725273836 34.0 31.0 34.0 30.0 34.0 4 35.9643584788318 37.0 35.0 37.0 35.0 37.0 5 35.92819233534473 37.0 35.0 37.0 35.0 37.0 6 35.87218161807763 37.0 35.0 37.0 35.0 37.0 7 35.807210868545596 37.0 35.0 37.0 35.0 37.0 8 35.77296338214243 37.0 35.0 37.0 33.0 37.0 9 37.41543479583438 39.0 37.0 39.0 34.0 39.0 10-11 37.411707941304236 39.0 37.0 39.0 34.0 39.0 12-13 37.428147529697 39.0 37.0 39.0 34.0 39.0 14-15 38.82754556124773 40.0 38.0 41.0 35.0 41.0 16-17 38.87297641540431 40.0 38.0 41.0 35.0 41.0 18-19 38.958125251231664 40.0 38.5 41.0 35.0 41.0 20-21 39.031620778164985 40.0 39.0 41.0 36.0 41.0 22-23 39.03813789678754 40.0 39.0 41.0 36.0 41.0 24-25 39.03966421489349 40.0 39.0 41.0 36.0 41.0 26-27 38.92940294127436 40.0 38.0 41.0 35.5 41.0 28-29 38.79214983106137 40.0 38.0 41.0 35.0 41.0 30-31 38.673904434856915 40.0 38.0 41.0 35.0 41.0 32-33 38.565291521393746 40.0 38.0 41.0 35.0 41.0 34-35 38.504608458989296 40.0 38.0 41.0 35.0 41.0 36-37 38.428612533072354 40.0 38.0 41.0 35.0 41.0 38-39 38.318829674042874 40.0 38.0 41.0 34.0 41.0 40-41 38.17256245998733 40.0 38.0 41.0 34.0 41.0 42-43 37.977408138973345 40.0 37.5 41.0 33.5 41.0 44-45 37.74007774359002 40.0 37.0 41.0 33.0 41.0 46-47 37.44232385375875 40.0 37.0 41.0 33.0 41.0 48-49 37.16317607302821 40.0 37.0 41.0 32.5 41.0 50-51 36.92164571205075 40.0 36.5 41.0 32.0 41.0 52-53 36.727435540522535 40.0 36.0 41.0 31.0 41.0 54-55 36.50124608468742 39.0 35.5 41.0 31.0 41.0 56-57 36.2592616931463 39.0 35.0 41.0 31.0 41.0 58-59 36.02075178146646 39.0 35.0 41.0 30.5 41.0 60-61 35.76205396892085 38.5 35.0 40.5 30.0 41.0 62-63 35.4708633444813 38.0 35.0 40.0 30.0 41.0 64-65 35.16268162594148 37.0 35.0 40.0 29.0 41.0 66-67 34.85384367230259 37.0 34.5 40.0 29.0 41.0 68-69 34.51993662347809 36.0 34.0 39.0 29.0 41.0 70-71 34.20083500658552 36.0 34.0 39.0 29.0 41.0 72-73 33.85788184988438 35.0 34.0 38.5 28.0 40.0 74-75 33.53018669263729 35.0 34.0 37.0 28.0 40.0 76-77 33.22704240906374 35.0 34.0 37.0 28.0 39.0 78-79 32.932626326628174 35.0 34.0 36.5 27.0 39.0 80-81 32.67075962702681 35.0 33.0 36.0 27.0 38.0 82-83 32.426848836473525 35.0 33.0 36.0 27.0 37.0 84-85 32.18403685109078 35.0 33.0 35.0 26.0 37.0 86-87 32.01023793810627 35.0 33.0 35.0 26.0 36.5 88-89 31.859588975542945 35.0 33.0 35.0 26.0 36.0 90-91 31.70228750205283 35.0 33.0 35.0 25.5 36.0 92-93 31.50797699025773 35.0 33.0 35.0 25.0 36.0 94-95 31.375403845190206 35.0 33.0 35.0 25.0 35.0 96-97 31.258337660481942 35.0 33.0 35.0 25.0 35.0 98-99 31.117546245219664 35.0 33.0 35.0 24.0 35.0 100-101 30.25038048224532 34.5 31.5 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.480992201115626 1101 2 0.45801472615309535 1101 3 0.4343277836535506 1101 4 0.30961527095891483 1101 5 0.31901330868765143 1101 6 0.27890990307801644 1101 7 0.2865479527571537 1101 8 0.28614509290055423 1101 9 0.3410260479446734 1101 10-11 0.3467890160220861 1101 12-13 0.3721676847121458 1101 14-15 0.5037162687684784 1101 16-17 0.4882132486560735 1101 18-19 0.5302768706680112 1101 20-21 0.5040938192209836 1101 22-23 0.5314997011535496 1101 24-25 0.4974694283429386 1101 26-27 0.5318775352602287 1101 28-29 0.5242124931578189 1101 30-31 0.531667518622811 1101 32-33 0.5727007063498277 1101 34-35 0.5209953423303659 1101 36-37 0.5754476815076544 1101 38-39 0.5679018583794431 1101 40-41 0.5772936590777178 1101 42-43 0.564072904650871 1101 44-45 0.5216316511975663 1101 46-47 0.532999780821342 1101 48-49 0.497501343877893 1101 50-51 0.47723118242276996 1101 52-53 0.48151050580300137 1101 54-55 0.5307677012199434 1101 56-57 0.5550129226118656 1101 58-59 0.5099714100207393 1101 60-61 0.5139987248893192 1101 62-63 0.5866783637261506 1101 64-65 0.5392092303642428 1101 66-67 0.4870616185949359 1101 68-69 0.48741107624856994 1101 70-71 0.5021090461466713 1101 72-73 0.5169568254005767 1101 74-75 0.4803639417875445 1101 76-77 0.41092490716198427 1101 78-79 0.45048100401982083 1101 80-81 0.4626610331330241 1101 82-83 0.4555565265224004 1101 84-85 0.44267073709725935 1101 86-87 0.4840146272213204 1101 88-89 0.47293540712225024 1101 90-91 0.4924013039912616 1101 92-93 0.490184070951166 1101 94-95 0.4804887141320826 1101 96-97 0.47094610904819234 1101 98-99 0.4997110694938556 1101 100-101 0.6666347648109863 1102 1 0.4526182255750655 1102 2 0.4254899372019523 1102 3 0.39018403499387944 1102 4 0.2718442365338305 1102 5 0.2587408865274412 1102 6 0.2393370794744598 1102 7 0.2637686958306773 1102 8 0.22910488866166645 1102 9 0.29386515115582057 1102 10-11 0.2960530955452896 1102 12-13 0.32210645393666937 1102 14-15 0.41017256156185766 1102 16-17 0.42917993033500323 1102 18-19 0.45231212895508577 1102 20-21 0.4594937085978472 1102 22-23 0.4985554040482185 1102 24-25 0.490358335652175 1102 26-27 0.4857504009802227 1102 28-29 0.49949708174874985 1102 30-31 0.46598247795607506 1102 32-33 0.47296704749538065 1102 34-35 0.4868288799810614 1102 36-37 0.4881054058968246 1102 38-39 0.5158742940170242 1102 40-41 0.5305900725958637 1102 42-43 0.5494682328129841 1102 44-45 0.483173666668236 1102 46-47 0.508494066770254 1102 48-49 0.4463210405137801 1102 50-51 0.4816036268921948 1102 52-53 0.4623812007782675 1102 54-55 0.4468028898287315 1102 56-57 0.4596884531354277 1102 58-59 0.4682354511292246 1102 60-61 0.45962831187155473 1102 62-63 0.46566640803642656 1102 64-65 0.4188813797481856 1102 66-67 0.4083514289559034 1102 68-69 0.3650503446475497 1102 70-71 0.35844986654476685 1102 72-73 0.38982370246277753 1102 74-75 0.35860743660242633 1102 76-77 0.3376850397837856 1102 78-79 0.3572985476345707 1102 80-81 0.3886265763913386 1102 82-83 0.3933849443522561 1102 84-85 0.39954803225308666 1102 86-87 0.3696952270993279 1102 88-89 0.38319442587600605 1102 90-91 0.43235708776918713 1102 92-93 0.46003579004433703 1102 94-95 0.4606491769426029 1102 96-97 0.43627448361269927 1102 98-99 0.42696834285084506 1102 100-101 0.5673737485735018 1103 1 0.35806931839710643 1103 2 0.33470481729343504 1103 3 0.32143159711289115 1103 4 0.24112011222727148 1103 5 0.22813295903425512 1103 6 0.19660413312556813 1103 7 0.198223122833447 1103 8 0.20364982507124552 1103 9 0.27689002344548896 1103 10-11 0.26072517801516426 1103 12-13 0.24149207158090036 1103 14-15 0.3526951699808194 1103 16-17 0.3515238430323322 1103 18-19 0.38254894005321916 1103 20-21 0.34550764449882365 1103 22-23 0.36701006638266165 1103 24-25 0.36484119630138423 1103 26-27 0.3673484316168967 1103 28-29 0.3557021870313051 1103 30-31 0.3673936271716016 1103 32-33 0.37920424388434526 1103 34-35 0.39035468371529447 1103 36-37 0.41859423144524044 1103 38-39 0.36109332112659587 1103 40-41 0.3409169814349937 1103 42-43 0.35704577797594084 1103 44-45 0.36136759839835264 1103 46-47 0.3009756213176331 1103 48-49 0.30148246404302625 1103 50-51 0.32488866893795887 1103 52-53 0.33013769443714125 1103 54-55 0.33190810467974785 1103 56-57 0.3391353019328136 1103 58-59 0.33651549742119613 1103 60-61 0.36968487801350136 1103 62-63 0.36635764441501806 1103 64-65 0.3221440668880149 1103 66-67 0.32404704426497233 1103 68-69 0.3044042744929172 1103 70-71 0.3055913890543138 1103 72-73 0.26905324472965475 1103 74-75 0.2885940695364724 1103 76-77 0.3225821599289773 1103 78-79 0.3450457654731309 1103 80-81 0.2990002797126152 1103 82-83 0.3134370114663767 1103 84-85 0.33050207563244527 1103 86-87 0.307327670296516 1103 88-89 0.3048840634698635 1103 90-91 0.3025530981921669 1103 92-93 0.33071161993952813 1103 94-95 0.3545473996953312 1103 96-97 0.38583515488430464 1103 98-99 0.35483755628352043 1103 100-101 0.48076415351294344 1104 1 0.3295036089225931 1104 2 0.35158039370295313 1104 3 0.3200573969678473 1104 4 0.25161592620221285 1104 5 0.2165794273719115 1104 6 0.18657158227126303 1104 7 0.21391356284229346 1104 8 0.181382016846797 1104 9 0.18221436030583504 1104 10-11 0.1975991357087281 1104 12-13 0.20345932966971247 1104 14-15 0.2847826330329468 1104 16-17 0.3376519670330964 1104 18-19 0.2885164922728265 1104 20-21 0.3393108240157048 1104 22-23 0.3704279843865095 1104 24-25 0.34687416115973235 1104 26-27 0.3695824233419316 1104 28-29 0.36157373387739966 1104 30-31 0.3111264487799943 1104 32-33 0.27200524318873676 1104 34-35 0.34678798336452843 1104 36-37 0.4186685471640388 1104 38-39 0.3588864503678906 1104 40-41 0.3789225667880274 1104 42-43 0.3532866767918108 1104 44-45 0.32861593505026576 1104 46-47 0.2978852904190603 1104 48-49 0.2838710623603049 1104 50-51 0.25797256056456774 1104 52-53 0.2768264522342463 1104 54-55 0.2470639342068921 1104 56-57 0.2666806793669707 1104 58-59 0.33534470452373455 1104 60-61 0.2930042251854985 1104 62-63 0.2686917701646152 1104 64-65 0.35389107995135305 1104 66-67 0.31683073170843556 1104 68-69 0.30926988974876934 1104 70-71 0.2664493925013076 1104 72-73 0.24645240385156342 1104 74-75 0.2541107218098091 1104 76-77 0.2716073071153815 1104 78-79 0.25443415422577687 1104 80-81 0.25495269829063716 1104 82-83 0.2843613424042388 1104 84-85 0.23896077047188413 1104 86-87 0.23682290996186595 1104 88-89 0.2128015846961091 1104 90-91 0.2100682539006833 1104 92-93 0.27510256987949333 1104 94-95 0.3004086121007603 1104 96-97 0.3384846526267822 1104 98-99 0.34507379484893974 1104 100-101 0.533334160486092 1105 1 0.2704536237870556 1105 2 0.2217454768796614 1105 3 0.18412791591691047 1105 4 0.1564932811316737 1105 5 0.17652669201875426 1105 6 0.12262794388789189 1105 7 0.12877759324472748 1105 8 0.12207137548325164 1105 9 0.15447135017632974 1105 10-11 0.16566403942414354 1105 12-13 0.18180316386663975 1105 14-15 0.22782124638316503 1105 16-17 0.2520885486401596 1105 18-19 0.21270539922200982 1105 20-21 0.2426172970371283 1105 22-23 0.24879201842977494 1105 24-25 0.2526431815355039 1105 26-27 0.2463278177185373 1105 28-29 0.2573395939256429 1105 30-31 0.2327888242154046 1105 32-33 0.2636967416424696 1105 34-35 0.22421888698986692 1105 36-37 0.3036835064672445 1105 38-39 0.30317628328270985 1105 40-41 0.3287349608276031 1105 42-43 0.26089140427502855 1105 44-45 0.26539129253225724 1105 46-47 0.2146032994855318 1105 48-49 0.1886671729144993 1105 50-51 0.24268178481045766 1105 52-53 0.2400630802640933 1105 54-55 0.22875600305681587 1105 56-57 0.28747229489350445 1105 58-59 0.29161418350883395 1105 60-61 0.25025878379278765 1105 62-63 0.2229248021363759 1105 64-65 0.24749696332965243 1105 66-67 0.24228732060254288 1105 68-69 0.2401442761008994 1105 70-71 0.1922437579757741 1105 72-73 0.18528980650551574 1105 74-75 0.1928342137816017 1105 76-77 0.18135987517928953 1105 78-79 0.18090935430718957 1105 80-81 0.1397812562526468 1105 82-83 0.1018295258483235 1105 84-85 0.12850842426468745 1105 86-87 0.18951686107428145 1105 88-89 0.14358057913930367 1105 90-91 0.17705658536550928 1105 92-93 0.1410135155262644 1105 94-95 0.13153544708869092 1105 96-97 0.1842307780454462 1105 98-99 0.19447353488491714 1105 100-101 0.2909952803306908 1106 1 0.29018283752265717 1106 2 0.2584546704494599 1106 3 0.23874802853437416 1106 4 0.15029764534823897 1106 5 0.17113955714802387 1106 6 0.1347105420544068 1106 7 0.13278860753649724 1106 8 0.08812965267754436 1106 9 0.14181080026940407 1106 10-11 0.12413895009788689 1106 12-13 0.15701223263529585 1106 14-15 0.15325983326474102 1106 16-17 0.19063661512458197 1106 18-19 0.224215447969641 1106 20-21 0.1971890250453896 1106 22-23 0.2150739710671985 1106 24-25 0.2275938303081375 1106 26-27 0.187091190451234 1106 28-29 0.21367202765966908 1106 30-31 0.1945638337419524 1106 32-33 0.11130967808667691 1106 34-35 0.13356919929345423 1106 36-37 0.18486092686838163 1106 38-39 0.2047918888818785 1106 40-41 0.1869369200122506 1106 42-43 0.14923346294832385 1106 44-45 0.11722670710190641 1106 46-47 0.1037503148049268 1106 48-49 0.11157750554714596 1106 50-51 0.11717850969331778 1106 52-53 0.10285199706318338 1106 54-55 0.06898624291129352 1106 56-57 0.07575377882852763 1106 58-59 0.05758018088908301 1106 60-61 0.09421303826597693 1106 62-63 0.13801807219704187 1106 64-65 0.11179631767167564 1106 66-67 0.08436274861033155 1106 68-69 0.0453798559837324 1106 70-71 0.11532882295184521 1106 72-73 0.09685156932491878 1106 74-75 0.09278855718365975 1106 76-77 0.022077322808989663 1106 78-79 0.08022720112870729 1106 80-81 0.08323822864130648 1106 82-83 0.09808818728408397 1106 84-85 0.14155915496357352 1106 86-87 0.1520559715192178 1106 88-89 0.12531913212480106 1106 90-91 0.11324889598245491 1106 92-93 0.15490299154618725 1106 94-95 0.19635196362881757 1106 96-97 0.1658988001255821 1106 98-99 0.20712436490919472 1106 100-101 0.35453775116996766 1107 1 0.23212457125729458 1107 2 0.2423235254986693 1107 3 0.1977432299150621 1107 4 0.17592299615681384 1107 5 0.17322007296369435 1107 6 0.1273962661895922 1107 7 0.05331342268524253 1107 8 0.10879669722305607 1107 9 0.09592711071344695 1107 10-11 0.15959946783608814 1107 12-13 0.08826163874675785 1107 14-15 0.14632209653801453 1107 16-17 0.15512072763966245 1107 18-19 0.12380026464315819 1107 20-21 0.20373378711430945 1107 22-23 0.19220127977432355 1107 24-25 0.19212327171040045 1107 26-27 0.15530728466459465 1107 28-29 0.14311019806313396 1107 30-31 0.1616457113578278 1107 32-33 0.2597148111619063 1107 34-35 0.2477075443830259 1107 36-37 0.26469841134441197 1107 38-39 0.22896567944860635 1107 40-41 0.23867126787293103 1107 42-43 0.270465074502134 1107 44-45 0.24993265935658115 1107 46-47 0.1942283895453869 1107 48-49 0.14017457787005583 1107 50-51 0.17636857556798446 1107 52-53 0.1621749420431442 1107 54-55 0.13138819605873664 1107 56-57 0.1665786568465819 1107 58-59 0.1552071404450004 1107 60-61 0.15457646213554455 1107 62-63 0.16584082437039172 1107 64-65 0.16082797552117256 1107 66-67 0.1233237696345384 1107 68-69 0.16221591899447674 1107 70-71 0.1407891694291692 1107 72-73 0.11481026120118543 1107 74-75 0.09084970449895025 1107 76-77 0.11789560175871117 1107 78-79 0.17370433006716013 1107 80-81 0.19560407889079556 1107 82-83 0.22002133311438143 1107 84-85 0.1481359593902809 1107 86-87 0.17851963051339226 1107 88-89 0.18224448006061067 1107 90-91 0.18605786165514715 1107 92-93 0.21952947157164004 1107 94-95 0.2280521863784486 1107 96-97 0.2993385499963068 1107 98-99 0.31155862669746526 1107 100-101 0.3964396253164253 1108 1 0.15618033451485758 1108 2 0.1255531832144854 1108 3 0.13841013332430663 1108 4 0.09560996089745544 1108 5 0.1087396933849476 1108 6 0.09199274561204618 1108 7 0.11771059455170274 1108 8 0.14195695869275227 1108 9 0.09079920861097435 1108 10-11 0.09975767470508856 1108 12-13 0.09411629069835215 1108 14-15 0.05790765432109879 1108 16-17 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-0.5375521893483963 2215 1 -0.558817014817091 2215 2 -0.5229929879996789 2215 3 -0.5063026469945839 2215 4 -0.3887636363293936 2215 5 -0.42476948547233206 2215 6 -0.36300747229603303 2215 7 -0.3350971916228431 2215 8 -0.2905950103931545 2215 9 -0.3850723857711529 2215 10-11 -0.4243868359925216 2215 12-13 -0.44239734349960713 2215 14-15 -0.5515882169851807 2215 16-17 -0.5714066820687336 2215 18-19 -0.5831760668441532 2215 20-21 -0.539153520684188 2215 22-23 -0.5620234893768838 2215 24-25 -0.5495025945097467 2215 26-27 -0.5063233125918387 2215 28-29 -0.5289498872599623 2215 30-31 -0.5555565828604045 2215 32-33 -0.5520231240706295 2215 34-35 -0.5990659215307161 2215 36-37 -0.612028536403308 2215 38-39 -0.606156417334752 2215 40-41 -0.6192448497593475 2215 42-43 -0.595753889049 2215 44-45 -0.6271022705880327 2215 46-47 -0.6594627890069091 2215 48-49 -0.6534559117721344 2215 50-51 -0.6432660812978099 2215 52-53 -0.6397199238363527 2215 54-55 -0.5905251486429393 2215 56-57 -0.6085203875090741 2215 58-59 -0.5323130427079619 2215 60-61 -0.5465464699518918 2215 62-63 -0.4887990676171796 2215 64-65 -0.5287552635290993 2215 66-67 -0.5355636016625169 2215 68-69 -0.4293709589872847 2215 70-71 -0.47532262936357483 2215 72-73 -0.4681165367285516 2215 74-75 -0.46262213876383385 2215 76-77 -0.5188655413589416 2215 78-79 -0.4553302230083318 2215 80-81 -0.44147557104453483 2215 82-83 -0.4497587959367664 2215 84-85 -0.46727959736742264 2215 86-87 -0.5228412676349379 2215 88-89 -0.5003493144826514 2215 90-91 -0.4606144374377372 2215 92-93 -0.5359436930134294 2215 94-95 -0.512722961588949 2215 96-97 -0.511029452358752 2215 98-99 -0.5531436184058656 2215 100-101 -0.6577233950076682 2216 1 -0.6288473457093673 2216 2 -0.6208384805297271 2216 3 -0.6102513678845973 2216 4 -0.44408123696889135 2216 5 -0.4680575321572036 2216 6 -0.4452821776714373 2216 7 -0.40761448619414153 2216 8 -0.4016257892791515 2216 9 -0.5049071434518879 2216 10-11 -0.4995043753152544 2216 12-13 -0.4840441483288487 2216 14-15 -0.7531727021646901 2216 16-17 -0.6976133150150119 2216 18-19 -0.7381301074776516 2216 20-21 -0.7475388457670533 2216 22-23 -0.7610954792431528 2216 24-25 -0.7395307958301771 2216 26-27 -0.6947588593755611 2216 28-29 -0.7113288084142155 2216 30-31 -0.6925381589115247 2216 32-33 -0.7476515467358382 2216 34-35 -0.8015453412212352 2216 36-37 -0.851197999297618 2216 38-39 -0.8004526579065825 2216 40-41 -0.8508113416775842 2216 42-43 -0.7522494507563096 2216 44-45 -0.7165820146279458 2216 46-47 -0.7696984626460335 2216 48-49 -0.7815591156789026 2216 50-51 -0.7464162446504901 2216 52-53 -0.8068140300069544 2216 54-55 -0.7623935555498775 2216 56-57 -0.7730647442598126 2216 58-59 -0.8100395296171143 2216 60-61 -0.8026690975040651 2216 62-63 -0.8134921666531909 2216 64-65 -0.7447699654621687 2216 66-67 -0.7650384777814452 2216 68-69 -0.702177931996296 2216 70-71 -0.7339288640240227 2216 72-73 -0.7247873573748791 2216 74-75 -0.7230142924133958 2216 76-77 -0.7312854708740204 2216 78-79 -0.7816387132000813 2216 80-81 -0.7571722691667375 2216 82-83 -0.7413986578972178 2216 84-85 -0.6894410418353587 2216 86-87 -0.692264795623597 2216 88-89 -0.7420068833110491 2216 90-91 -0.7803047974864903 2216 92-93 -0.7239713909248167 2216 94-95 -0.7654084606851121 2216 96-97 -0.7700536311290449 2216 98-99 -0.8490336327967505 2216 100-101 -1.068294076104884 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 265.0 3 68.0 4 83.0 5 160.0 6 352.0 7 856.0 8 2094.0 9 3664.0 10 5770.0 11 6801.0 12 7924.0 13 11610.0 14 19892.0 15 35609.0 16 48855.0 17 66878.0 18 64857.0 19 41839.0 20 32408.0 21 28063.0 22 25840.0 23 26040.0 24 28743.0 25 33690.0 26 41467.0 27 52836.0 28 67161.0 29 85513.0 30 111814.0 31 148777.0 32 203301.0 33 281056.0 34 398674.0 35 589229.0 36 1045684.0 37 1965371.0 38 2187670.0 39 530724.0 40 453.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 24.61265702114672 36.6334069417565 17.670289127152632 21.083646909944157 2 23.648873255203387 28.86495329689827 28.921157008708953 18.56501643918939 3 22.66489167018813 27.858975318705664 24.399804830041933 25.076328181064273 4 23.801503787265954 26.529722725583028 26.402011389534692 23.26676209761633 5 23.513924318548604 26.96172889613853 25.324755681144158 24.199591104168704 6 24.699238279604554 25.876499053716913 25.96013626281396 23.464126403864576 7 23.965047449485745 27.41093704032301 24.685814873304867 23.938200636886375 8 25.237807271867176 25.9496441120602 25.83724497136071 22.97530364471191 9 23.49458205230837 26.091785619057383 26.127374104969086 24.28625822366516 10-11 24.394340418802962 26.132183854092816 25.576142717753314 23.89733300935091 12-13 23.980592266045328 26.257901796017624 25.61918661960712 24.142319318329925 14-15 23.441489742702696 26.307558898877875 26.129311592932897 24.12163976548653 16-17 23.499166249191823 26.324579915048492 26.153586932893084 24.0226669028666 18-19 22.92815941690966 26.400011899404674 27.158349011246035 23.513479672439626 20-21 22.095115037156997 26.258414167415157 28.093233321413607 23.553237474014242 22-23 21.823286584349194 26.077422804128247 28.86775527149129 23.231535340031268 24-25 21.83210702622492 25.68839832946937 29.90078290654749 22.57871173775822 26-27 21.750215574422924 25.637291887768637 30.483204947871954 22.129287589936478 28-29 21.847181960950575 25.76299491794992 30.41254916478171 21.97727395631779 30-31 21.834187268445646 25.635251153489097 30.319711745385497 22.210849832679767 32-33 21.840252999948675 25.591519298146792 30.37505103902594 22.193176662878592 34-35 21.966614466528057 25.76339883313823 30.252409585342654 22.01757711499106 36-37 21.937070681122655 25.644911767011607 30.162784099810647 22.255233452055094 38-39 21.962771444501165 25.680652653085655 30.164277621401663 22.192298281011514 40-41 22.0550893668456 25.945621671351855 29.78422819254261 22.215060769259935 42-43 22.262646195951742 25.913142148752065 29.375088620694406 22.449123034601786 44-45 22.210153484032304 26.048040676456775 29.185960019219486 22.555845820291434 46-47 22.509162602560735 26.32515903566542 28.46252254455602 22.70315581721783 48-49 22.71240593929579 26.390178788109143 27.855178504339783 23.042236768255286 50-51 22.783446318749697 26.349232165310042 27.66872496293933 23.198596553000936 52-53 22.886048848704714 26.53485463683517 27.358956688083303 23.220139826376812 54-55 22.95380120713032 26.459242693887848 27.184079296270834 23.402876802711 56-57 22.93416109036753 26.411981855007223 27.295205698545583 23.358651356079662 58-59 23.084072745149584 26.620062953846364 27.049627363756475 23.246236937247577 60-61 23.04782587412276 26.53259410275713 26.947556322419118 23.472023700700987 62-63 23.022161308389144 26.53696628993607 27.110770695935056 23.330101705739732 64-65 23.113222470892012 26.732673448396334 26.902813082988093 23.25129099772357 66-67 23.134153074602786 26.579464584474177 26.829517635174433 23.456864705748597 68-69 23.107894146009333 26.608461045481008 26.910409157003812 23.37323565150584 70-71 23.153621810881667 26.813526730017333 26.768617340669206 23.264234118431794 72-73 23.13839226440728 26.722519419998797 26.666667073072453 23.472421242521474 74-75 23.086524830996044 26.671894642629194 26.851478299205795 23.390102227168967 76-77 23.158887296609468 26.839930076256284 26.680836246619457 23.320346380514792 78-79 23.154722410996904 26.774295057630937 26.621271740686254 23.449710790685902 80-81 23.08259290757971 26.738989840442823 26.794482528398778 23.383934723578687 82-83 23.1333294318887 26.91774291674439 26.62627452271311 23.322653128653794 84-85 23.110000296276898 26.80198839594347 26.59802065086548 23.48999065691416 86-87 23.05627207408302 26.752564759026136 26.743298163147323 23.447865003743523 88-89 23.120261859152677 26.931441082483172 26.533521685424454 23.4147753729397 90-91 23.10682696413205 26.85585859307543 26.49039529534433 23.546919147448193 92-93 23.077407016644838 26.82471640909561 26.647891774984405 23.449984799275146 94-95 23.14043868461308 27.00408035469513 26.488752155684676 23.36672880500711 96-97 23.12752291072389 26.95415392358492 26.3387612237065 23.579561941984696 98-99 23.09854691889771 26.913657494418096 26.520507132997988 23.4672884536862 100-101 23.189010638651318 27.042230596264965 26.313768127206604 23.45499063787711 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 634.0 1 458.5 2 231.0 3 151.0 4 120.5 5 107.0 6 112.5 7 130.0 8 134.0 9 137.0 10 148.0 11 154.5 12 185.0 13 406.5 14 630.0 15 868.5 16 3588.5 17 4205.5 18 2421.0 19 2857.5 20 3825.0 21 5495.0 22 8087.0 23 12064.0 24 17534.0 25 24451.0 26 33266.5 27 44118.0 28 56184.0 29 69407.5 30 83918.5 31 100737.5 32 120460.5 33 140175.0 34 155604.0 35 172898.0 36 194297.5 37 218716.0 38 238592.0 39 251762.5 40 265009.0 41 280403.5 42 302346.0 43 322215.5 44 333174.5 45 336671.5 46 336536.0 47 335902.0 48 338186.0 49 333594.0 50 321777.0 51 307953.0 52 278412.5 53 244182.0 54 222861.0 55 206056.5 56 185599.0 57 165335.5 58 152881.5 59 143532.5 60 128290.0 61 112686.5 62 99966.0 63 88748.5 64 78007.5 65 66277.0 66 55448.5 67 45671.0 68 36367.5 69 28597.0 70 22149.5 71 16788.0 72 12616.0 73 9342.0 74 6761.5 75 4591.5 76 3070.5 77 2133.0 78 1463.0 79 871.5 80 378.5 81 153.5 82 65.5 83 27.0 84 14.5 85 6.5 86 5.5 87 5.5 88 2.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2942176574241861 2 0.019080500326075386 3 1.0972811689116835E-4 4 0.0 5 2.438402597581519E-5 6 0.0 7 0.0 8 8.656329221414393E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 4.2672045457676584E-5 16-17 0.0 18-19 0.0 20-21 2.438402597581519E-5 22-23 0.0037612360067694932 24-25 3.230883441795513E-4 26-27 0.009107433701966974 28-29 0.011844540617752228 30-31 0.0055229818835221415 32-33 4.1452844158885825E-4 34-35 6.705607143349177E-5 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 9.144009740930697E-5 50-51 0.0 52-53 0.0016276337338856641 54-55 0.002956563149567592 56-57 0.005967990357580768 58-59 0.006425190844627303 60-61 0.0017007858118131096 62-63 0.0041635724353704445 64-65 5.4864058445584176E-5 66-67 3.8404840911908925E-4 68-69 2.377442532641981E-4 70-71 2.4384025975815191E-4 72-73 0.0013167374026940204 74-75 7.802888312260861E-4 76-77 1.5849616884279876E-4 78-79 8.900169481172544E-4 80-81 0.0013350254221758816 82-83 1.2192012987907595E-5 84-85 0.0037063719483239094 86-87 0.007187191656371528 88-89 0.005242565584800267 90-91 0.002109218246908014 92-93 0.0021884663313294136 94-95 0.0028102589937127006 96-97 0.003108963311916437 98-99 0.0017068818183070635 100-101 0.001932434058583354 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8202091.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.830150157486386 #Duplication Level Percentage of deduplicated Percentage of total 1 77.49850487806339 37.84263630176527 2 13.115399615106178 12.808538651621475 3 3.8216275119538596 5.598319357640641 4 1.5231541071267327 2.9750337505596183 5 0.7779258099002156 1.8993117054405866 6 0.5018633013869142 1.4703636219152918 7 0.3293117501271456 1.1256239545133209 8 0.24987291693289254 0.9761065643297812 9 0.2128176536411573 0.9352726185115475 >10 1.6646835877531414 16.081642341300142 >50 0.17718750281770423 6.142141224587037 >100 0.12600405312022345 10.058498723122026 >500 9.735174541797801E-4 0.3062724488114054 >1k 4.991071331655095E-4 0.43869874422841165 >5k 9.982141884561222E-5 0.30128016598668306 >10k+ 7.486606413420917E-5 1.040259825666768 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37897 0.4620407162027341 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 31110 0.3792935240538053 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16360 0.19946133248216827 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.2192012987907595E-5 2.682242857339671E-4 0.0 0.0 0.0 2 1.2192012987907595E-5 3.5356837664932025E-4 0.0 2.438402597581519E-5 0.0 3 1.2192012987907595E-5 3.657603896372279E-4 0.0 2.438402597581519E-5 0.0 4 2.438402597581519E-5 4.023364286009507E-4 0.0 2.438402597581519E-5 0.0 5 3.657603896372279E-5 4.7548850652839625E-4 0.0 2.438402597581519E-5 0.0 6 3.657603896372279E-5 5.12064545492119E-4 0.0 2.438402597581519E-5 0.0 7 4.876805195163038E-5 5.364485714679342E-4 0.0 2.438402597581519E-5 0.0 8 6.096006493953798E-5 5.974086364074722E-4 0.0 3.657603896372279E-5 0.0 9 9.753610390326076E-5 6.217926623832874E-4 0.0 3.657603896372279E-5 0.0 10-11 1.4020814936093734E-4 6.888487338167792E-4 0.0 3.657603896372279E-5 0.0 12-13 1.8288019481861394E-4 0.0010302250974781918 0.0 3.657603896372279E-5 0.0 14-15 2.1945623378233673E-4 0.001865377987149862 0.0 4.2672045457676584E-5 0.0 16-17 3.352803571674589E-4 0.003474723701553665 0.0 4.876805195163038E-5 0.0 18-19 4.5110448055258104E-4 0.009119625714954883 0.0 4.876805195163038E-5 0.0 20-21 5.852166234195646E-4 0.01758088272856275 0.0 6.705607143349177E-5 0.0 22-23 9.570730195507463E-4 0.03774037620406796 0.0 7.924808442139937E-5 0.0 24-25 0.0013716014611396044 0.059478735361507204 0.0 8.534409091535317E-5 0.0 26-27 0.0017434578572707862 0.06359963575141997 0.0 8.534409091535317E-5 0.0 28-29 0.0024201145780996577 0.06462986084889816 0.0 8.534409091535317E-5 0.0 30-31 0.0033832836041443578 0.06531870958271493 0.0 8.534409091535317E-5 0.0 32-33 0.004852421169187222 0.06587344617366475 0.0 8.534409091535317E-5 0.0 34-35 0.006839719286216161 0.06631845464772337 0.0 8.534409091535317E-5 0.0 36-37 0.009576826202001416 0.06672079107632431 0.0 8.534409091535317E-5 0.0 38-39 0.01377087866984163 0.06711093549193736 0.0 8.534409091535317E-5 0.0 40-41 0.018854948085799097 0.0674766958815746 0.0 1.2192012987907596E-4 0.0 42-43 0.024889994514813357 0.0678058802322481 0.0 1.2192012987907596E-4 0.0 44-45 0.03274165087902585 0.0680558164985002 0.0 1.4020814936093737E-4 0.0 46-47 0.04216607691867842 0.0682813687387765 0.0 1.4630415585489115E-4 0.0 48-49 0.0537789692896604 0.06832404078423417 0.0 1.4630415585489115E-4 0.0 50-51 0.06752546393352622 0.06832404078423417 0.0 1.5849616884279873E-4 0.0 52-53 0.08326535270091492 0.06833623279722208 0.0 1.5849616884279873E-4 0.0 54-55 0.10128514789704235 0.06837890484267975 0.0 1.5849616884279873E-4 0.0 56-57 0.12165800159983595 0.0683850008491737 0.0 1.5849616884279873E-4 0.0 58-59 0.1451642026405218 0.0683850008491737 0.0 1.5849616884279873E-4 0.0 60-61 0.1715903907918115 0.06840938487514953 0.0 1.5849616884279873E-4 0.0 62-63 0.20174733491740093 0.06842157688813742 0.0 1.5849616884279873E-4 0.0 64-65 0.2354338668029896 0.06842157688813742 0.0 1.7678418832466015E-4 0.0 66-67 0.2723817621628436 0.06843986490761929 0.0 1.8288019481861394E-4 0.0 68-69 0.31304822148400935 0.06844596091411324 0.0 1.8288019481861394E-4 0.0 70-71 0.3589328623639996 0.06844596091411324 0.0 1.8288019481861394E-4 0.0 72-73 0.40887744356896305 0.06844596091411324 0.0 1.8897620131256773E-4 0.0 74-75 0.46380855808598076 0.06844596091411324 0.0 1.9507220780652152E-4 0.0 76-77 0.5246588949086275 0.06844596091411324 0.0 1.9507220780652152E-4 0.0 78-79 0.5917942144265407 0.06844596091411324 0.0 1.9507220780652152E-4 0.0 80-81 0.6631235864122942 0.06844596091411324 0.0 2.0726422079442913E-4 0.0 82-83 0.7422375586908265 0.06844596091411324 0.0 2.0726422079442913E-4 0.0 84-85 0.8270939690866634 0.06844596091411324 0.0 2.0726422079442913E-4 0.0 86-87 0.9180890580219117 0.06844596091411324 0.0 2.1336022728838294E-4 0.0 88-89 1.017655132087659 0.06844596091411324 0.0 2.1945623378233673E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 2860 0.0 81.46893 1 TCGACGG 4435 0.0 58.26956 2 TATCTCG 10610 0.0 57.802593 2 ATATCTC 11395 0.0 54.19258 1 ATCTCGA 20990 0.0 49.078632 1 TCTCGAG 21755 0.0 45.17913 2 CGACGGC 6445 0.0 40.164394 3 GACGGCG 6845 0.0 37.956093 4 ACGGCGC 7000 0.0 36.97993 5 CTCGAGG 27170 0.0 36.27405 3 TCGAGGG 27720 0.0 35.52006 4 CGGCGCG 7835 0.0 33.281353 6 CGAGGGC 33280 0.0 29.628635 5 AGGGCGC 34360 0.0 28.61441 7 GGGCGCG 35240 0.0 27.92682 8 GAGGGCG 36100 0.0 27.366783 6 CTCGACG 1885 0.0 26.961252 3 TCTCGAC 2025 0.0 26.03961 2 GGCGCGC 43495 0.0 23.805473 9 CGCGCCG 43080 0.0 17.629194 9 >>END_MODULE