FastQCFastQC Report
Thu 26 May 2016
SRR2048270_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048270_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9046652
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT796050.8799387884048154No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG160960.1779221749659432No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC150810.1667025547130585No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA137830.15235470536503448No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC113870.12586976928039234No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG112380.12422275113489498No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA104700.1157334227070965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG308950.091.705361
TATCTCG366400.087.233872
TCGACGG339750.083.264552
ATATCTC391450.082.199171
ATCTCGA392400.081.40543
TCTCGAG366650.079.736654
CGACGGC357450.079.2345663
ACGGCGC359750.077.816555
GACGGCG362100.077.667574
CTCGAGG374400.077.424385
TCGAGGG377900.076.650216
CGGCGCG368800.075.721286
AGGGCGC392850.073.849269
CGAGGGC421450.068.804737
CTCGACG37750.068.6061865
GAGGGCG423000.068.563468
TCTCGAC40200.064.899254
CGCGCCG759750.038.8108989
GGCGCGC761650.038.4027527
GCGCGCC766400.038.3566178
Produced by FastQC (version 0.11.5)