FastQCFastQC Report
Thu 26 May 2016
SRR2048270_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048270_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9046652
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT505190.558427581828062No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT498230.5507341279403696No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228810.25292229655788684No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118800.13131929911750778No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT111150.12286313212888039No Hit
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91090.10068918313647966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG38700.079.162931
TATCTCG145750.059.6827742
TCGACGG56300.059.4064372
ATATCTC151300.057.8799931
ATCTCGA261550.043.5012251
CGACGGC79000.042.3266533
TCTCGAG269100.040.428812
GACGGCG85050.039.4274564
ACGGCGC85600.039.0631525
CGGCGCG98650.033.9919436
CTCGAGG330100.033.0653343
TCGAGGG336750.032.5252084
TATACAT107050.029.2017172
CGAGGGC401800.027.3185985
ACATATA86200.026.5587835
AGGGCGC414250.026.428767
GAGGGCG422800.025.9729466
GGGCGCG424400.025.8078788
CATATGT83000.025.0075844
CTCGACG22050.024.340993
Produced by FastQC (version 0.11.5)