##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048270_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9046652 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24531174626812 34.0 31.0 34.0 30.0 34.0 2 32.4016747853239 34.0 31.0 34.0 30.0 34.0 3 32.473174274858806 34.0 31.0 34.0 30.0 34.0 4 35.94660698786689 37.0 35.0 37.0 35.0 37.0 5 35.91057222052976 37.0 35.0 37.0 35.0 37.0 6 35.846611984190396 37.0 35.0 37.0 35.0 37.0 7 35.77551308484067 37.0 35.0 37.0 33.0 37.0 8 35.734767845607415 37.0 35.0 37.0 33.0 37.0 9 37.3624755323848 39.0 37.0 39.0 34.0 39.0 10-11 37.35128625484876 39.0 37.0 39.0 34.0 39.0 12-13 37.36636874061254 39.0 37.0 39.0 34.0 39.0 14-15 38.75713772343625 40.0 38.0 41.0 34.0 41.0 16-17 38.79690873485572 40.0 38.0 41.0 35.0 41.0 18-19 38.87306193495671 40.0 38.0 41.0 35.0 41.0 20-21 38.9460664564084 40.0 38.0 41.0 35.0 41.0 22-23 38.95576783543791 40.0 38.0 41.0 35.0 41.0 24-25 38.96291909979515 40.0 38.0 41.0 35.0 41.0 26-27 38.847216517226485 40.0 38.0 41.0 35.0 41.0 28-29 38.699298536077215 40.0 38.0 41.0 35.0 41.0 30-31 38.56766265575375 40.0 38.0 41.0 35.0 41.0 32-33 38.44670188485199 40.0 38.0 41.0 35.0 41.0 34-35 38.37583135728002 40.0 38.0 41.0 34.5 41.0 36-37 38.29760446184953 40.0 38.0 41.0 34.0 41.0 38-39 38.1844501148049 40.0 38.0 41.0 34.0 41.0 40-41 38.03423222204192 40.0 37.0 41.0 34.0 41.0 42-43 37.83779093083275 40.0 37.0 41.0 33.0 41.0 44-45 37.60781568695248 40.0 37.0 41.0 33.0 41.0 46-47 37.318170744270915 40.0 37.0 41.0 33.0 41.0 48-49 37.03798991052159 40.0 36.5 41.0 32.0 41.0 50-51 36.785139850632035 40.0 36.0 41.0 31.0 41.0 52-53 36.58039946711778 40.0 36.0 41.0 31.0 41.0 54-55 36.34707762606542 39.0 35.5 41.0 31.0 41.0 56-57 36.09875741876664 39.0 35.0 41.0 30.0 41.0 58-59 35.84949255260399 39.0 35.0 41.0 30.0 41.0 60-61 35.584008481811836 38.5 35.0 40.0 29.5 41.0 62-63 35.2818567023469 38.0 35.0 40.0 29.0 41.0 64-65 34.96087232050044 37.0 35.0 40.0 29.0 41.0 66-67 34.638260817372 37.0 34.0 40.0 28.0 41.0 68-69 34.287349839476526 36.0 34.0 39.0 28.0 41.0 70-71 33.9558186277089 36.0 34.0 39.0 28.0 41.0 72-73 33.59779838994581 35.0 34.0 38.5 27.0 40.0 74-75 33.26179884005707 35.0 34.0 37.0 26.0 40.0 76-77 32.954044048560725 35.0 33.5 37.0 26.0 39.0 78-79 32.65411364336774 35.0 33.0 36.5 26.0 39.0 80-81 32.387897423267745 35.0 33.0 36.0 26.0 38.5 82-83 32.13479246244909 35.0 33.0 36.0 26.0 37.0 84-85 31.884122656646902 35.0 33.0 35.0 25.0 37.0 86-87 31.70300106602973 35.0 33.0 35.0 25.0 36.5 88-89 31.549042286582925 35.0 33.0 35.0 25.0 36.0 90-91 31.387946004776133 35.0 33.0 35.0 24.5 36.0 92-93 31.188167456866914 35.0 33.0 35.0 23.5 36.0 94-95 31.054501212161142 35.0 33.0 35.0 23.0 35.0 96-97 30.935032540214877 35.0 33.0 35.0 22.0 35.0 98-99 30.790141866847534 35.0 33.0 35.0 19.5 35.0 100-101 29.92152251462751 34.5 31.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.49163768863800783 1101 2 0.4299020731767982 1101 3 0.434328545010608 1101 4 0.3098066446309744 1101 5 0.3092044498762334 1101 6 0.26130838620226626 1101 7 0.28381904440526284 1101 8 0.2865420681689841 1101 9 0.3328103782363456 1101 10-11 0.3472947945501943 1101 12-13 0.3342351115610498 1101 14-15 0.4465110410201376 1101 16-17 0.46553375645262207 1101 18-19 0.5102874256045098 1101 20-21 0.49925503380866587 1101 22-23 0.4976266302994148 1101 24-25 0.5125680917691042 1101 26-27 0.5232311081919931 1101 28-29 0.4931529692655374 1101 30-31 0.5099527376827666 1101 32-33 0.5197871174452828 1101 34-35 0.4864157931433297 1101 36-37 0.5444324610008238 1101 38-39 0.5390154222689887 1101 40-41 0.5301299040482093 1101 42-43 0.5274513397580094 1101 44-45 0.49703897590880075 1101 46-47 0.5013304414068074 1101 48-49 0.48009216661813525 1101 50-51 0.4848956031698677 1101 52-53 0.4875036815470821 1101 54-55 0.4915622420768031 1101 56-57 0.4594892244783537 1101 58-59 0.4807950454009813 1101 60-61 0.45950856760424585 1101 62-63 0.5211346500846759 1101 64-65 0.45898577582962474 1101 66-67 0.4271665706865875 1101 68-69 0.41204533283969624 1101 70-71 0.3919904017438469 1101 72-73 0.38715941656169406 1101 74-75 0.35662810889263596 1101 76-77 0.3689290724772789 1101 78-79 0.41885584283603094 1101 80-81 0.4214357013553425 1101 82-83 0.3866825645389582 1101 84-85 0.3958219791987858 1101 86-87 0.4226380745213092 1101 88-89 0.4166407419049136 1101 90-91 0.42656993888962447 1101 92-93 0.4797491153519893 1101 94-95 0.4871413515131806 1101 96-97 0.4491616971406991 1101 98-99 0.49929594261039867 1101 100-101 0.6107112844425657 1102 1 0.41851378071588385 1102 2 0.3986155874916619 1102 3 0.3624293254381996 1102 4 0.25314696088145894 1102 5 0.28345807653766286 1102 6 0.25035155619595884 1102 7 0.22978554657226624 1102 8 0.21998045577603875 1102 9 0.2677779312594524 1102 10-11 0.2685653236770946 1102 12-13 0.2966892690745979 1102 14-15 0.38527742831315237 1102 16-17 0.3778710401251928 1102 18-19 0.4276004793066477 1102 20-21 0.4463400219953684 1102 22-23 0.44993055994516595 1102 24-25 0.4872362294292003 1102 26-27 0.44708549043970436 1102 28-29 0.45441550558456356 1102 30-31 0.4481591484063827 1102 32-33 0.44791550962404614 1102 34-35 0.4819212880313728 1102 36-37 0.5318934641205715 1102 38-39 0.5104949261309031 1102 40-41 0.49311165468194673 1102 42-43 0.4408802383805934 1102 44-45 0.40807706864485027 1102 46-47 0.36764860034155333 1102 48-49 0.33355534255551333 1102 50-51 0.35972706964400203 1102 52-53 0.37300000708320624 1102 54-55 0.3994182816848024 1102 56-57 0.39174163146528684 1102 58-59 0.3632434025238638 1102 60-61 0.333428333510156 1102 62-63 0.382636570386893 1102 64-65 0.31077659267823776 1102 66-67 0.28171530792721455 1102 68-69 0.288328556392635 1102 70-71 0.3487313838327992 1102 72-73 0.2852537371072188 1102 74-75 0.3247606155570537 1102 76-77 0.31324789306849965 1102 78-79 0.3379154733362455 1102 80-81 0.3071794928491727 1102 82-83 0.3300690665532642 1102 84-85 0.32307619629731477 1102 86-87 0.3493650403526445 1102 88-89 0.3186084064290782 1102 90-91 0.3147668817096978 1102 92-93 0.35582175746084843 1102 94-95 0.41083594597411377 1102 96-97 0.3256114956867293 1102 98-99 0.34243117697683445 1102 100-101 0.49095080117866274 1103 1 0.41291298307998403 1103 2 0.32496201963836313 1103 3 0.33025492716697613 1103 4 0.2633198686575895 1103 5 0.28320832825102116 1103 6 0.24580885824130405 1103 7 0.21257298199293473 1103 8 0.20568931800077905 1103 9 0.25873565933808607 1103 10-11 0.24251071912823363 1103 12-13 0.25219706360609706 1103 14-15 0.3696108308213226 1103 16-17 0.32895216636332947 1103 18-19 0.3622138551866598 1103 20-21 0.3419319596974333 1103 22-23 0.35130639923880835 1103 24-25 0.37212490618787086 1103 26-27 0.3416522374580282 1103 28-29 0.4117098686901528 1103 30-31 0.3491730592683737 1103 32-33 0.3491053628634262 1103 34-35 0.37489889694698064 1103 36-37 0.3743466453023956 1103 38-39 0.36804484538847504 1103 40-41 0.2972026088762689 1103 42-43 0.25910492342733704 1103 44-45 0.24051799721267741 1103 46-47 0.22008104116262928 1103 48-49 0.24197024409355095 1103 50-51 0.22914289782040953 1103 52-53 0.22101857102283162 1103 54-55 0.20814972364037487 1103 56-57 0.18593518030748157 1103 58-59 0.12499188775935721 1103 60-61 0.11750358664944827 1103 62-63 0.19028248233831135 1103 64-65 0.15430319434742756 1103 66-67 0.1437463698287118 1103 68-69 0.10469026366184409 1103 70-71 0.15218041271002392 1103 72-73 0.11126410245050522 1103 74-75 0.0803493809352176 1103 76-77 0.10006916808876554 1103 78-79 0.11647332494874973 1103 80-81 0.11215000777136197 1103 82-83 0.14948515002045326 1103 84-85 0.12153414590788714 1103 86-87 0.1736835667981218 1103 88-89 0.17431174026470586 1103 90-91 0.1412124573759712 1103 92-93 0.20271076717244796 1103 94-95 0.192592265277586 1103 96-97 0.19612798656441655 1103 98-99 0.24931227468958284 1103 100-101 0.3901848146178395 1104 1 0.31350764723710256 1104 2 0.3112209420306158 1104 3 0.2789657849495839 1104 4 0.21171397587853136 1104 5 0.21280443240649305 1104 6 0.19747256191757856 1104 7 0.19057232979194083 1104 8 0.1523230306608312 1104 9 0.17883050859779104 1104 10-11 0.1616690484242156 1104 12-13 0.1743997846666545 1104 14-15 0.2403966909533679 1104 16-17 0.28293699939194283 1104 18-19 0.28424853554268026 1104 20-21 0.3256668138940526 1104 22-23 0.3813557840407995 1104 24-25 0.34504793075993945 1104 26-27 0.34110043832369286 1104 28-29 0.3252470801093068 1104 30-31 0.33717739847956096 1104 32-33 0.32566277476315264 1104 34-35 0.32899788267661023 1104 36-37 0.3799505387759723 1104 38-39 0.3408935962941939 1104 40-41 0.3716981561369792 1104 42-43 0.3812743715934701 1104 44-45 0.35999935178818276 1104 46-47 0.26715701467522024 1104 48-49 0.24567304464767403 1104 50-51 0.3051297533540662 1104 52-53 0.2727153213340259 1104 54-55 0.29230227837948064 1104 56-57 0.3428826553103477 1104 58-59 0.3354488628093364 1104 60-61 0.31712015326851173 1104 62-63 0.31464229460920023 1104 64-65 0.32755215370506363 1104 66-67 0.2735116810405316 1104 68-69 0.33084303274199556 1104 70-71 0.33922155882977734 1104 72-73 0.2771362115909426 1104 74-75 0.24229523754526383 1104 76-77 0.2693922955674779 1104 78-79 0.2885678987454696 1104 80-81 0.27665165033570815 1104 82-83 0.3270890471906682 1104 84-85 0.3084278536851883 1104 86-87 0.2801850540400963 1104 88-89 0.2908891464939245 1104 90-91 0.30698357510781094 1104 92-93 0.36365041589085934 1104 94-95 0.40917009853178854 1104 96-97 0.39120374159318416 1104 98-99 0.39493558250349636 1104 100-101 0.532755196541018 1105 1 0.293348409330207 1105 2 0.2621336993899064 1105 3 0.21599571788148353 1105 4 0.14354708022325724 1105 5 0.17563427000975196 1105 6 0.13872622426825387 1105 7 0.14897105539060362 1105 8 0.14759738987245896 1105 9 0.1891993526808733 1105 10-11 0.17069993045532783 1105 12-13 0.1788826437085831 1105 14-15 0.29142531070292677 1105 16-17 0.2892240778281234 1105 18-19 0.28525398760810816 1105 20-21 0.27907527427705503 1105 22-23 0.3088067562943593 1105 24-25 0.31761940376485853 1105 26-27 0.29923253697120344 1105 28-29 0.33754107175548853 1105 30-31 0.29004069601146654 1105 32-33 0.31686990179658636 1105 34-35 0.3221078776780004 1105 36-37 0.38573530230367226 1105 38-39 0.3578411924836473 1105 40-41 0.3636010176119129 1105 42-43 0.35435792939608035 1105 44-45 0.36929148126315425 1105 46-47 0.3311146415613706 1105 48-49 0.31608414149899744 1105 50-51 0.32492045708361417 1105 52-53 0.35618521921010426 1105 54-55 0.3311378397542484 1105 56-57 0.3750257545982194 1105 58-59 0.3669261016709271 1105 60-61 0.39930493448395055 1105 62-63 0.4029971037063831 1105 64-65 0.43469100384967163 1105 66-67 0.40404685196502044 1105 68-69 0.39355506550683117 1105 70-71 0.33972125225247396 1105 72-73 0.3677438114979381 1105 74-75 0.3760572602662293 1105 76-77 0.3728085751993788 1105 78-79 0.3402712931866674 1105 80-81 0.3703556861549586 1105 82-83 0.33472952531739963 1105 84-85 0.31891253770601224 1105 86-87 0.338705227856984 1105 88-89 0.31047733134335687 1105 90-91 0.3088668547513542 1105 92-93 0.29685940584191073 1105 94-95 0.362175930251464 1105 96-97 0.3742138860817761 1105 98-99 0.37463379827232757 1105 100-101 0.5091233182712642 1106 1 0.2905558202554346 1106 2 0.26531278455965435 1106 3 0.22692310247246894 1106 4 0.14718977751149254 1106 5 0.19623933872505006 1106 6 0.11467805438361012 1106 7 0.13647061870706523 1106 8 0.10157525199667816 1106 9 0.09014017976234356 1106 10-11 0.08661792471242791 1106 12-13 0.16149750232103344 1106 14-15 0.16812037765549093 1106 16-17 0.17983968561735253 1106 18-19 0.2293775790739616 1106 20-21 0.19758055982043743 1106 22-23 0.21750515851783092 1106 24-25 0.18186622445649192 1106 26-27 0.2069132071123434 1106 28-29 0.22541489243310764 1106 30-31 0.2305305760435843 1106 32-33 0.1336026284073455 1106 34-35 0.1426403221375665 1106 36-37 0.21863647980656253 1106 38-39 0.2512163851737057 1106 40-41 0.1994752607290451 1106 42-43 0.18413976896560058 1106 44-45 0.09500817765444225 1106 46-47 0.0577112936963502 1106 48-49 0.05811508572087831 1106 50-51 0.07383485571191528 1106 52-53 0.08296489291041809 1106 54-55 0.0645331169305905 1106 56-57 0.1019445641100134 1106 58-59 0.027685082073027445 1106 60-61 0.0024463897937607726 1106 62-63 0.11308086265326978 1106 64-65 0.058076702337011454 1106 66-67 0.016537726539922915 1106 68-69 -0.004546889551903632 1106 70-71 -0.007301114420542376 1106 72-73 -0.020471239757313242 1106 74-75 -0.007104398606919915 1106 76-77 -0.03877446603281243 1106 78-79 0.03633848149900132 1106 80-81 0.004590847381535923 1106 82-83 0.03673154297217707 1106 84-85 0.08147011572812346 1106 86-87 0.09772559382660262 1106 88-89 0.09430182684531019 1106 90-91 0.06625412862711144 1106 92-93 0.1120291561117952 1106 94-95 0.1593505503443211 1106 96-97 0.1617826798996269 1106 98-99 0.13551665650497924 1106 100-101 0.25969465049445617 1107 1 0.22410708194258433 1107 2 0.21278924127652488 1107 3 0.15470687186368082 1107 4 0.14207399083726102 1107 5 0.15129170417139903 1107 6 0.1530084782790837 1107 7 0.05774109890078449 1107 8 0.07666578706403726 1107 9 0.1009946362605163 1107 10-11 0.13603700312966538 1107 12-13 0.10962350886895678 1107 14-15 0.1124311849114008 1107 16-17 0.15681983014537337 1107 18-19 0.1436852035070686 1107 20-21 0.1693321905946732 1107 22-23 0.11801860834417965 1107 24-25 0.10725862972501687 1107 26-27 0.09984597627675385 1107 28-29 0.11547337791088808 1107 30-31 0.09951096711770901 1107 32-33 0.21511909711535537 1107 34-35 0.21510812341107766 1107 36-37 0.15886174376207407 1107 38-39 0.13094350216042017 1107 40-41 0.13823015194576982 1107 42-43 0.14571143624432636 1107 44-45 0.12429349060861483 1107 46-47 0.12724656844417837 1107 48-49 0.08158898775845103 1107 50-51 0.1322382197738321 1107 52-53 0.16356346637658703 1107 54-55 0.12962981239431315 1107 56-57 0.13092363408867413 1107 58-59 0.1516102225343019 1107 60-61 0.2029656327460998 1107 62-63 0.17529633547918877 1107 64-65 0.2446513200735012 1107 66-67 0.1890110059918868 1107 68-69 0.2233340021383583 1107 70-71 0.2069191746662682 1107 72-73 0.20290822629847582 1107 74-75 0.14606722250644566 1107 76-77 0.19369027379340054 1107 78-79 0.2242742312124264 1107 80-81 0.23372699870977698 1107 82-83 0.21145293313468017 1107 84-85 0.16620633638299864 1107 86-87 0.21501794467376456 1107 88-89 0.2334920704419048 1107 90-91 0.21018829343018908 1107 92-93 0.2126350669012922 1107 94-95 0.24638547261476162 1107 96-97 0.271264806098916 1107 98-99 0.24711617895693294 1107 100-101 0.286477348738412 1108 1 0.18023155026740056 1108 2 0.1371246987052288 1108 3 0.12247766760667389 1108 4 0.08142175323306589 1108 5 0.079649719971151 1108 6 0.05518072450653477 1108 7 0.06757385777719094 1108 8 0.13144381425941987 1108 9 0.10713849764704975 1108 10-11 0.08684684660224917 1108 12-13 0.10246651864380141 1108 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88-89 -0.4558112881625469 2214 90-91 -0.44347281185626386 2214 92-93 -0.4072672597395268 2214 94-95 -0.37217039809548425 2214 96-97 -0.4453414311732615 2214 98-99 -0.5046869945082086 2214 100-101 -0.5585057560099713 2215 1 -0.5126355254253774 2215 2 -0.47812987085102066 2215 3 -0.46432100913332874 2215 4 -0.3878410640542924 2215 5 -0.3809654214819105 2215 6 -0.3263180900324443 2215 7 -0.32577704999584967 2215 8 -0.3158423265203183 2215 9 -0.3463473094341225 2215 10-11 -0.37160275913299046 2215 12-13 -0.4373672293501514 2215 14-15 -0.5391599224467214 2215 16-17 -0.5132316049139902 2215 18-19 -0.5666186423415169 2215 20-21 -0.5250816087402228 2215 22-23 -0.5376457968109847 2215 24-25 -0.5294823388323238 2215 26-27 -0.4827369333818936 2215 28-29 -0.5061444080154303 2215 30-31 -0.5890215169451096 2215 32-33 -0.5863013939017918 2215 34-35 -0.6535597657477652 2215 36-37 -0.6760862705956896 2215 38-39 -0.6839248835256484 2215 40-41 -0.6321395783796149 2215 42-43 -0.6244573743473083 2215 44-45 -0.6209788339658502 2215 46-47 -0.6253771315348473 2215 48-49 -0.6310608170909688 2215 50-51 -0.6204189866033474 2215 52-53 -0.6297887118033003 2215 54-55 -0.5526945937140226 2215 56-57 -0.6343854960456667 2215 58-59 -0.5561309269311181 2215 60-61 -0.6056682011887347 2215 62-63 -0.5695990877526498 2215 64-65 -0.582771787956716 2215 66-67 -0.6530267559378871 2215 68-69 -0.5689836123755043 2215 70-71 -0.5723373245652681 2215 72-73 -0.5039298248797763 2215 74-75 -0.4798374679279078 2215 76-77 -0.5063463710343328 2215 78-79 -0.4884672553021687 2215 80-81 -0.4875368780537812 2215 82-83 -0.4739830887688612 2215 84-85 -0.4402556070732473 2215 86-87 -0.5061066354688641 2215 88-89 -0.49918431964885457 2215 90-91 -0.48358766127865493 2215 92-93 -0.43767050510638583 2215 94-95 -0.4820003278118108 2215 96-97 -0.46098987662672997 2215 98-99 -0.5316255096639502 2215 100-101 -0.603512885894407 2216 1 -0.6327624379687364 2216 2 -0.5944538417133458 2216 3 -0.5697693884563719 2216 4 -0.4290403720754554 2216 5 -0.4407707547143076 2216 6 -0.4177465347368994 2216 7 -0.3371180339763953 2216 8 -0.37713462619143456 2216 9 -0.48109218566580836 2216 10-11 -0.47805016093128927 2216 12-13 -0.4296487096899355 2216 14-15 -0.6494634896912217 2216 16-17 -0.595780710495994 2216 18-19 -0.6618218334396673 2216 20-21 -0.7015529282610942 2216 22-23 -0.7536487506023022 2216 24-25 -0.6841804234890887 2216 26-27 -0.672431243342956 2216 28-29 -0.6573847730608975 2216 30-31 -0.6161237371631998 2216 32-33 -0.6679931523523095 2216 34-35 -0.7283868010043548 2216 36-37 -0.74895912003646 2216 38-39 -0.7278850283825022 2216 40-41 -0.7473902561212071 2216 42-43 -0.6719989290713073 2216 44-45 -0.6129294488011041 2216 46-47 -0.5536981203888374 2216 48-49 -0.5547703211251829 2216 50-51 -0.4554757082897751 2216 52-53 -0.5836248876019567 2216 54-55 -0.5965443927324259 2216 56-57 -0.5412945017019197 2216 58-59 -0.5624325374386174 2216 60-61 -0.5306187378514124 2216 62-63 -0.6213249248757435 2216 64-65 -0.5585845262579952 2216 66-67 -0.5320545605579099 2216 68-69 -0.4917753409761616 2216 70-71 -0.46783547726153074 2216 72-73 -0.5089940656836944 2216 74-75 -0.5207373214286548 2216 76-77 -0.5772531025411283 2216 78-79 -0.6065659829802357 2216 80-81 -0.5970649028856556 2216 82-83 -0.5412064116208484 2216 84-85 -0.5361831718953489 2216 86-87 -0.48142185876321264 2216 88-89 -0.5088425164659327 2216 90-91 -0.5764920582982249 2216 92-93 -0.4768475526765954 2216 94-95 -0.5365547812713913 2216 96-97 -0.5773808248762862 2216 98-99 -0.6676809127010443 2216 100-101 -0.834443428259803 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 355.0 3 118.0 4 153.0 5 294.0 6 741.0 7 1728.0 8 3514.0 9 5853.0 10 8988.0 11 10342.0 12 11430.0 13 15167.0 14 23733.0 15 40396.0 16 54797.0 17 72859.0 18 74658.0 19 51388.0 20 41964.0 21 37845.0 22 35761.0 23 35413.0 24 37579.0 25 41689.0 26 50610.0 27 62986.0 28 78158.0 29 97838.0 30 127919.0 31 170299.0 32 230767.0 33 320000.0 34 451227.0 35 659044.0 36 1141067.0 37 2100568.0 38 2358736.0 39 590147.0 40 521.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 23.884221044202565 37.898131786764026 17.009568388719437 21.20807878031397 2 23.031444265732194 28.591825101194523 30.6741680114309 17.702562621642382 3 22.047528795767533 28.766463843711293 24.673431312966734 24.51257604755444 4 23.245560899214425 26.373314680392262 27.365847608595978 23.015276811797335 5 23.11644386414376 27.63361823065795 25.438010154254876 23.811927750943415 6 24.300260472050876 25.726556078425478 26.749652799731876 23.22353064979177 7 23.561987351784946 28.124537121578236 24.761359229911793 23.55211629672502 8 24.928337527922167 25.744469509790225 26.84927424475293 22.477918717534678 9 23.246909464407388 26.569586185032872 26.33825198537536 23.84525236518438 10-11 24.305809486205504 26.165143745995756 26.05268225195354 23.476364515845198 12-13 23.764775079222677 26.346978970783887 25.94328266412812 23.944963285865313 14-15 23.284497576450953 26.292495011965755 26.428666965119685 23.994340446463607 16-17 23.297104829499354 26.43406643695369 26.445230788141295 23.82359794540566 18-19 22.805398063283523 26.507756681698382 27.4092890939101 23.277556161108 20-21 21.90970327529473 26.477542547096018 28.405543200155332 23.207210977453922 22-23 21.511074327525197 26.140721203810774 29.284120462215213 23.06408400644882 24-25 21.482097344875474 25.714109326368227 30.407050412977487 22.396742915778812 26-27 21.466545867130865 25.62652967405295 31.070412970469686 21.836511488346495 28-29 21.57832752738814 25.76814307664942 31.010385918566268 21.64314347739617 30-31 21.531653751947292 25.650332280313204 30.91452463063921 21.90348933710029 32-33 21.538383736219277 25.610485922619592 30.97631100285687 21.874819338304256 34-35 21.629178551827778 25.782558064343192 30.8407315640218 21.747531819807232 36-37 21.63123440583323 25.708350448320548 30.685440315378553 21.97497483046767 38-39 21.65191609006293 25.662322370751077 30.790462593233386 21.8952989459526 40-41 21.76249843588545 25.930736586308395 30.405729102876954 21.9010358749292 42-43 21.937402919886825 25.90416874662582 29.998501102949465 22.159927230537882 44-45 21.919213870501487 26.0164478527526 29.803141537886056 22.261196738859855 46-47 22.206060319331396 26.332177915100523 29.115942560850133 22.345819204717944 48-49 22.402774156115147 26.384925325338916 28.5149474783077 22.69735304023824 50-51 22.4959299860324 26.32196419183583 28.277930885370633 22.904174936761134 52-53 22.622405730833545 26.507934972659324 28.00049521900867 22.869164077498457 54-55 22.65929377520275 26.399600812833153 27.762316718184493 23.1787886937796 56-57 22.64524522235063 26.421173573404204 27.84051901224024 23.093062192004925 58-59 22.826531120414977 26.627178772291394 27.578448902110846 22.967841205182786 60-61 22.789598469374134 26.553205050508232 27.39622210611833 23.260974373999304 62-63 22.791005059977103 26.548320493073067 27.536731648767237 23.123942798182593 64-65 22.835078161037192 26.798050945527528 27.306676078629387 23.06019481480589 66-67 22.860570819534882 26.68324157333241 27.184832943444988 23.271354663687724 68-69 22.84536546051915 26.68708458964872 27.28452272874741 23.18302722108472 70-71 22.88768083881876 26.924611069109574 27.05971115310221 23.12799693896946 72-73 22.902921876097622 26.854960521618587 26.916315514666472 23.325802087617316 74-75 22.853831896859994 26.78622533141541 27.113525922567177 23.246416849157423 76-77 22.91124855823392 26.958291266566043 26.933823593215177 23.196636581984862 78-79 22.926271502742566 26.88133746463223 26.875583889747134 23.316807142878066 80-81 22.864296042251386 26.857395266765298 27.02481877146233 23.253489919520984 82-83 22.900324750081495 27.03987410144906 26.848615437605684 23.21118571086377 84-85 22.880910577503318 26.91304945235639 26.848962498824104 23.35707747131618 86-87 22.856718674379277 26.848343228351734 26.98391747216384 23.311020625105158 88-89 22.932136418727616 26.970269555616376 26.78825285635389 23.309341169302115 90-91 22.896926547980417 26.901367402805725 26.731450043450717 23.47025600576314 92-93 22.845760177112552 26.855918414856834 26.95286250928679 23.34545889874383 94-95 22.93146550835519 27.017428450575267 26.773529961382923 23.277576079686625 96-97 22.905893830390898 26.975315605401875 26.61821710300033 23.5005734612069 98-99 22.868339141014424 26.920930108136336 26.829209517739823 23.381521233109414 100-101 22.979696371907604 27.065912618656885 26.566999341842145 23.387391667593363 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8279.0 1 4987.5 2 1328.0 3 861.0 4 684.5 5 591.0 6 588.5 7 574.0 8 588.0 9 618.0 10 623.5 11 638.5 12 730.5 13 1082.0 14 1368.0 15 1804.5 16 6310.0 17 7082.0 18 3971.0 19 4624.0 20 5918.0 21 8142.0 22 11674.0 23 16741.0 24 23303.5 25 31550.0 26 41785.0 27 54090.5 28 67828.0 29 82452.0 30 98926.0 31 118466.5 32 141771.0 33 164345.5 34 181228.5 35 200312.0 36 223276.5 37 250698.0 38 274540.0 39 288398.0 40 299246.5 41 313517.0 42 336206.0 43 356592.5 44 367199.0 45 369554.0 46 368486.0 47 366970.0 48 366704.0 49 357812.5 50 342254.0 51 326960.0 52 296048.5 53 259635.5 54 235874.5 55 217174.0 56 196237.5 57 175450.5 58 161104.5 59 149208.5 60 132759.0 61 116781.0 62 104171.5 63 92868.0 64 81119.5 65 68947.0 66 57748.0 67 47707.5 68 38365.0 69 30204.0 70 23313.0 71 17206.5 72 12727.5 73 9903.5 74 7547.0 75 5185.5 76 3484.0 77 2415.0 78 1633.5 79 969.0 80 428.0 81 162.5 82 62.0 83 30.0 84 15.0 85 7.5 86 6.0 87 3.0 88 2.0 89 1.5 90 1.5 91 2.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2921412252842267 2 0.01874726694472165 3 1.1053813057029276E-4 4 0.0 5 1.1053813057029274E-5 6 0.0 7 0.0 8 8.400897923342248E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.210762611405855E-5 16-17 0.0 18-19 0.0 20-21 5.526906528514637E-6 22-23 0.0038301462242606435 24-25 3.6477583088196606E-4 26-27 0.008970169295779255 28-29 0.011484911766253415 30-31 0.0049686889691346586 32-33 5.195292136803759E-4 34-35 7.737669139920492E-5 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 9.948431751326348E-5 50-51 0.0 52-53 0.0016525450520258765 54-55 0.002973475712340875 56-57 0.006029855022609469 58-59 0.006505168984061728 60-61 0.0017299217434250816 62-63 0.004045695578872714 64-65 9.948431751326348E-5 66-67 4.974215875663173E-4 68-69 2.763453264257319E-4 70-71 2.818722329542465E-4 72-73 0.001309876847257969 74-75 8.290359792771957E-4 76-77 2.3765698072612942E-4 78-79 0.0011330158383455005 80-81 0.0014646302300563789 82-83 3.3161439171087825E-5 84-85 0.0038577807569032166 86-87 0.007582915757122082 88-89 0.005571121780742754 90-91 0.002161020452649223 92-93 0.0022218164244628843 94-95 0.0029347873666412724 96-97 0.00296794880581236 98-99 0.0017188679303680521 100-101 0.0021002244808355623 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9046652.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.44044611352141 #Duplication Level Percentage of deduplicated Percentage of total 1 78.03589195420008 38.58129311082209 2 12.95035480923559 12.805426381939903 3 3.7338438675343277 5.538087195873999 4 1.518912414550109 3.003828295308934 5 0.7834953882880645 1.9368180762424296 6 0.46110196887793936 1.3678252227089034 7 0.3259272462259884 1.1279791917775084 8 0.23836829505849733 0.9428027877609287 9 0.18606401250580926 0.8279179005563133 >10 1.481313087022899 14.837181245627962 >50 0.15345984712928962 5.367995315563552 >100 0.1295212398878223 10.830347264052278 >500 0.0010298283185916622 0.3231591313367819 >1k 4.699020691043445E-4 0.4166412151621865 >5k 1.34257688682341E-4 0.4750976736193997 >10k+ 1.1188140723528417E-4 1.6175999916470225 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 50519 0.558427581828062 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 49823 0.5507341279403696 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22881 0.25292229655788684 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11880 0.13131929911750778 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 11115 0.12286313212888039 No Hit CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9109 0.10068918313647966 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.210762611405855E-5 1.326457566843513E-4 0.0 0.0 0.0 2 2.210762611405855E-5 4.0899108311008315E-4 0.0 0.0 0.0 3 2.210762611405855E-5 7.295516617639321E-4 0.0 0.0 0.0 4 3.3161439171087825E-5 0.0011827579971021324 0.0 0.0 0.0 5 4.42152522281171E-5 0.0017465024630106253 0.0 0.0 0.0 6 5.526906528514637E-5 0.0021886549852917964 0.0 0.0 0.0 7 1.4369956974138057E-4 0.0028739913948276114 0.0 0.0 0.0 8 1.6580719585543912E-4 0.0034487896737931335 0.0 1.1053813057029274E-5 0.0 9 0.008655135623653921 0.003935157448302422 0.0 2.210762611405855E-5 0.0 10-11 0.01837696420731117 0.004554170979496061 0.0 3.3161439171087825E-5 0.0 12-13 0.036284141359698596 0.005151076884575642 0.0 3.3161439171087825E-5 0.0 14-15 0.044132348630189375 0.005543487248100181 0.0 3.3161439171087825E-5 0.0 16-17 0.05575543305965566 0.006051962648723527 0.0 4.42152522281171E-5 0.0 18-19 0.0702304012578355 0.006682029992974197 0.0 4.42152522281171E-5 0.0 20-21 0.08365525721559755 0.007262355178468233 0.0 5.526906528514637E-5 0.0 22-23 0.10392795036218924 0.008135606409973545 0.0 5.526906528514637E-5 0.0 24-25 0.11909930878296192 0.009384687285417853 0.0 5.526906528514637E-5 0.0 26-27 0.12693646224039568 0.010926694206873437 0.0 6.0795971813661014E-5 0.0 28-29 0.13437015152124787 0.013386167612062452 0.0 6.632287834217565E-5 0.0 30-31 0.1394770131535954 0.01671336534222826 0.0 6.632287834217565E-5 0.0 32-33 0.14418593751588984 0.021107256032397402 0.0 6.632287834217565E-5 0.0 34-35 0.1492983260547659 0.026694958532725695 0.0 6.632287834217565E-5 0.0 36-37 0.15464284466783956 0.0415733909074871 0.0 6.632287834217565E-5 0.0 38-39 0.16083297997977594 0.07987485315009354 0.0 6.632287834217565E-5 0.0 40-41 0.16740447184217985 0.1231726388944772 0.0 6.632287834217565E-5 0.0 42-43 0.17539637868241204 0.16469076073667915 0.0 6.632287834217565E-5 0.0 44-45 0.18443287085653343 0.20058801863938175 0.0 7.737669139920492E-5 0.0 46-47 0.19503347757822453 0.2320029553474589 0.0 7.737669139920492E-5 0.0 48-49 0.20684447682966028 0.2595711651116899 0.0 8.84305044562342E-5 0.0 50-51 0.2204351399832778 0.28236965454181284 0.0 8.84305044562342E-5 0.0 52-53 0.23577230559990592 0.30212834538125266 0.0 8.84305044562342E-5 0.0 54-55 0.2528449198664876 0.3185377308644126 0.0 8.84305044562342E-5 0.0 56-57 0.27203986624001897 0.332460008409741 0.0 8.84305044562342E-5 5.526906528514637E-6 58-59 0.2942580304846478 0.3449729247902981 0.0 9.948431751326347E-5 1.1053813057029274E-5 60-61 0.31760921056762215 0.3552198094941642 0.0 9.948431751326347E-5 1.1053813057029274E-5 62-63 0.3438067475127815 0.36351569619346474 0.0 9.948431751326347E-5 1.1053813057029274E-5 64-65 0.3729445987311107 0.36987716560778505 0.0 9.948431751326347E-5 1.1053813057029274E-5 66-67 0.40480168796146904 0.3750614039315318 0.0 9.948431751326347E-5 1.1053813057029274E-5 68-69 0.4401352013982631 0.37875337749257953 0.0 1.1053813057029274E-4 1.1053813057029274E-5 70-71 0.47764631600729196 0.3812681199630537 0.0 1.1053813057029274E-4 1.1053813057029274E-5 72-73 0.5187609736729124 0.38273275019311015 0.0 1.1053813057029274E-4 1.1053813057029274E-5 74-75 0.5659828630525414 0.3835783668919729 0.0 1.326457566843513E-4 1.1053813057029274E-5 76-77 0.6171454367870015 0.3840149925077255 0.0 1.326457566843513E-4 1.1053813057029274E-5 78-79 0.670065566797529 0.38418079970358093 0.0 1.326457566843513E-4 1.1053813057029274E-5 80-81 0.7279764934033055 0.38425817639498017 0.0 1.5475338279840985E-4 1.1053813057029274E-5 82-83 0.7916685642379081 0.3843079185537368 0.0 1.7133410238395376E-4 1.1053813057029274E-5 84-85 0.8613683824690062 0.3843466068994364 0.0 1.8791482196949766E-4 1.1053813057029274E-5 86-87 0.9359429322582542 0.3843521338059649 0.0 1.934417284980123E-4 1.1053813057029274E-5 88-89 1.0166191868549823 0.3843521338059649 0.0 1.9896863502652694E-4 1.1053813057029274E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 3870 0.0 79.16293 1 TATCTCG 14575 0.0 59.682774 2 TCGACGG 5630 0.0 59.406437 2 ATATCTC 15130 0.0 57.879993 1 ATCTCGA 26155 0.0 43.501225 1 CGACGGC 7900 0.0 42.326653 3 TCTCGAG 26910 0.0 40.42881 2 GACGGCG 8505 0.0 39.427456 4 ACGGCGC 8560 0.0 39.063152 5 CGGCGCG 9865 0.0 33.991943 6 CTCGAGG 33010 0.0 33.065334 3 TCGAGGG 33675 0.0 32.525208 4 TATACAT 10705 0.0 29.201717 2 CGAGGGC 40180 0.0 27.318598 5 ACATATA 8620 0.0 26.558783 5 AGGGCGC 41425 0.0 26.42876 7 GAGGGCG 42280 0.0 25.972946 6 GGGCGCG 42440 0.0 25.807878 8 CATATGT 8300 0.0 25.007584 4 CTCGACG 2205 0.0 24.34099 3 >>END_MODULE