FastQCFastQC Report
Thu 26 May 2016
SRR2048269_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048269_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7834313
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT623070.7953090462431103No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG125900.16070330608440075No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC125660.16039696141831453No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA117710.15024929435420822No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC96470.12313779140557697No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA91910.11731724274993863No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG85410.10902040804343661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG225450.085.5730061
TATCTCG305100.081.7202762
TCGACGG243600.079.041132
ATATCTC328100.076.4116441
ATCTCGA332750.074.815453
CGACGGC259550.074.3120043
TCTCGAG320450.073.417434
GACGGCG265350.072.437924
ACGGCGC266000.071.9957055
CGGCGCG273250.069.8210456
CTCGAGG336200.069.44365
TCGAGGG338700.068.752956
AGGGCGC354550.065.8087169
CGAGGGC376200.061.895247
GAGGGCG383950.060.732868
CTCGACG22450.055.45855
TCTCGAC25150.050.0696334
CGCGCCG625550.033.20719
GGCGCGC626250.032.8969767
GGGCGCG355750.032.89454310-11
Produced by FastQC (version 0.11.5)