##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2048269_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7834313
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.99439516904673	31.0	31.0	34.0	28.0	34.0
2	31.148068375618895	33.0	31.0	34.0	28.0	34.0
3	31.188296408376843	33.0	31.0	34.0	28.0	34.0
4	34.71490978213406	37.0	35.0	37.0	32.0	37.0
5	34.64976380188027	37.0	35.0	37.0	32.0	37.0
6	34.62058153663251	37.0	35.0	37.0	32.0	37.0
7	34.54703890436851	37.0	35.0	37.0	31.0	37.0
8	34.31630865399429	37.0	35.0	37.0	30.0	37.0
9	35.90694882882519	39.0	37.0	39.0	30.0	39.0
10-11	35.66614685933534	39.0	37.0	39.0	30.0	39.0
12-13	35.6973677845141	39.0	37.0	39.0	30.0	39.0
14-15	36.96124950585967	40.0	37.0	41.0	30.5	41.0
16-17	36.93619121421368	40.0	37.0	41.0	30.0	41.0
18-19	36.92458336806303	40.0	37.0	41.0	30.0	41.0
20-21	36.95222899570135	40.0	37.0	41.0	30.0	41.0
22-23	37.02184046003778	40.0	38.0	41.0	31.0	41.0
24-25	37.05619254681298	40.0	38.0	41.0	31.0	41.0
26-27	36.97127201836332	40.0	37.0	41.0	30.5	41.0
28-29	36.88085726214921	40.0	37.0	41.0	30.0	41.0
30-31	36.69415288870894	40.0	36.5	41.0	30.0	41.0
32-33	36.4284970488159	40.0	35.5	41.0	30.0	41.0
34-35	36.36897510987882	40.0	35.0	41.0	30.0	41.0
36-37	36.25808963721516	40.0	35.0	41.0	30.0	41.0
38-39	36.124202339120224	40.0	35.0	41.0	30.0	41.0
40-41	35.74236062562218	40.0	35.0	41.0	27.5	41.0
42-43	35.58860164254351	39.0	35.0	41.0	27.0	41.0
44-45	35.36976937224745	39.0	35.0	41.0	26.0	41.0
46-47	35.0282443399951	39.0	35.0	41.0	23.5	41.0
48-49	34.521987250190286	39.0	34.5	41.0	20.5	41.0
50-51	34.17864048832361	39.0	34.0	41.0	17.0	41.0
52-53	33.957354461074	39.0	34.0	41.0	14.0	41.0
54-55	33.73615018955715	38.5	33.5	40.0	10.0	41.0
56-57	33.494166176408825	38.0	33.0	40.0	7.5	41.0
58-59	33.25733985864491	38.0	33.0	40.0	6.5	41.0
60-61	32.98759048815129	37.5	33.0	40.0	2.0	41.0
62-63	32.68429484499789	37.0	32.5	40.0	2.0	41.0
64-65	32.380088273215534	36.5	32.0	40.0	2.0	41.0
66-67	32.06687835934051	36.0	32.0	39.0	2.0	41.0
68-69	31.712208396575424	35.5	31.5	39.0	2.0	41.0
70-71	31.38319160850479	35.0	31.0	39.0	2.0	40.5
72-73	31.039256218126592	35.0	31.0	37.5	2.0	40.0
74-75	30.683055042605524	35.0	31.0	37.0	2.0	39.5
76-77	30.34445668688499	35.0	31.0	37.0	2.0	39.0
78-79	30.009057782092697	35.0	30.5	36.0	2.0	39.0
80-81	29.68575681109499	35.0	30.0	36.0	2.0	37.5
82-83	29.434196208397594	35.0	30.0	35.0	2.0	37.0
84-85	29.17204029249278	34.0	30.0	35.0	2.0	37.0
86-87	28.8833044454568	34.0	30.0	35.0	2.0	36.0
88-89	28.642887385275518	34.0	29.0	35.0	2.0	36.0
90-91	28.4314999285834	34.0	29.0	35.0	2.0	36.0
92-93	28.220211395689706	34.0	29.0	35.0	2.0	35.5
94-95	28.009645963851582	34.0	29.0	35.0	2.0	35.0
96-97	27.7908851101558	34.0	29.0	35.0	2.0	35.0
98-99	27.504584511749783	34.0	29.0	35.0	2.0	35.0
100-101	26.262921841391837	33.0	24.0	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7316174361460632
1101	2	0.7852170775807465
1101	3	0.7538626879569001
1101	4	0.7610566795581803
1101	5	0.790326699598431
1101	6	0.7546988970205817
1101	7	0.7989336880057323
1101	8	0.9282956049667206
1101	9	1.025211932073013
1101	10-11	1.1556790955627605
1101	12-13	1.088172631761708
1101	14-15	1.2020891275787093
1101	16-17	1.141693254603318
1101	18-19	1.1253713971512695
1101	20-21	1.1384751423666941
1101	22-23	1.0816048195860333
1101	24-25	1.1086626223434877
1101	26-27	1.1575524040671041
1101	28-29	1.220118337152094
1101	30-31	1.13915231707945
1101	32-33	1.1439880145645276
1101	34-35	1.1254513655438458
1101	36-37	1.0790826004538019
1101	38-39	1.072850215212327
1101	40-41	1.1079239308383464
1101	42-43	0.9713884828282033
1101	44-45	0.895621010929176
1101	46-47	0.8662558166431893
1101	48-49	0.6860503748312468
1101	50-51	0.5903146961922658
1101	52-53	0.5336443238687423
1101	54-55	0.5393223315332492
1101	56-57	0.5114982405963886
1101	58-59	0.5436697820489655
1101	60-61	0.5001622640660628
1101	62-63	0.46874602094644047
1101	64-65	0.49524475801521817
1101	66-67	0.47145691324569583
1101	68-69	0.4409874937077518
1101	70-71	0.4165814904418852
1101	72-73	0.4700231497360612
1101	74-75	0.5095409898978858
1101	76-77	0.5056928777587792
1101	78-79	0.45532228508213635
1101	80-81	0.516503157905511
1101	82-83	0.46571649859761166
1101	84-85	0.4572991061423828
1101	86-87	0.5069976700793681
1101	88-89	0.5188333684908528
1101	90-91	0.5357866539494864
1101	92-93	0.5740424210148447
1101	94-95	0.6505402650027534
1101	96-97	0.6411482816339564
1101	98-99	0.6797441631586274
1101	100-101	0.7049895930843313
1102	1	0.6168733479182578
1102	2	0.6400458124707313
1102	3	0.6020348799497484
1102	4	0.6842870383037578
1102	5	0.6579006160666552
1102	6	0.6614682123529718
1102	7	0.7136010447665768
1102	8	0.8228342576850238
1102	9	0.8431890635673298
1102	10-11	1.0148826584942796
1102	12-13	0.9532777812322166
1102	14-15	1.0397758277600957
1102	16-17	1.0464141655951096
1102	18-19	1.0776289796553442
1102	20-21	1.0902899722513624
1102	22-23	1.0455991587212026
1102	24-25	0.9858704728319481
1102	26-27	1.0509903235916198
1102	28-29	1.084689899466234
1102	30-31	1.0755841861585225
1102	32-33	1.0706724313298963
1102	34-35	1.019259318881133
1102	36-37	0.9676303579306165
1102	38-39	1.0409540672591575
1102	40-41	1.00812067155546
1102	42-43	0.8707863023046087
1102	44-45	0.8719660418307811
1102	46-47	0.855664805322661
1102	48-49	0.7474237302908833
1102	50-51	0.6290700055394254
1102	52-53	0.5759454924503942
1102	54-55	0.5742893872033648
1102	56-57	0.5379481830425803
1102	58-59	0.5218985977588986
1102	60-61	0.5315197024185778
1102	62-63	0.448329280108581
1102	64-65	0.4572328191092154
1102	66-67	0.49539065988303577
1102	68-69	0.5129770983586468
1102	70-71	0.4711660529604558
1102	72-73	0.45563813679684273
1102	74-75	0.4477233085982135
1102	76-77	0.4926878020277776
1102	78-79	0.4531388775614964
1102	80-81	0.4597694907101193
1102	82-83	0.45398514872777795
1102	84-85	0.42952182512955517
1102	86-87	0.40739830871975613
1102	88-89	0.428964893242167
1102	90-91	0.42494900522931545
1102	92-93	0.4630462892705829
1102	94-95	0.5625578064506982
1102	96-97	0.6216949062402861
1102	98-99	0.5861100706032332
1102	100-101	0.5815894743554537
1103	1	0.5348550230260294
1103	2	0.5725950724769149
1103	3	0.5458592160754101
1103	4	0.6032044390525613
1103	5	0.6159908098281832
1103	6	0.5898657907744536
1103	7	0.5944151726224334
1103	8	0.7713570004863968
1103	9	0.7391313759961946
1103	10-11	0.8998689023928037
1103	12-13	0.818084350890679
1103	14-15	0.9289984430186351
1103	16-17	0.8833781033327739
1103	18-19	0.9002928001392974
1103	20-21	0.912776806426244
1103	22-23	0.8867691620979201
1103	24-25	0.848083874728971
1103	26-27	0.8941383582366313
1103	28-29	0.9457903108431438
1103	30-31	0.9226009112052367
1103	32-33	0.9504643195851514
1103	34-35	0.9204979929996853
1103	36-37	0.8817636827403135
1103	38-39	0.8680057472703879
1103	40-41	0.9313061332491728
1103	42-43	0.8671594151624191
1103	44-45	0.8580222425749398
1103	46-47	0.8115166126925146
1103	48-49	0.6339919957268378
1103	50-51	0.5560764502745243
1103	52-53	0.5262144648224378
1103	54-55	0.5408679325914818
1103	56-57	0.5400254885740097
1103	58-59	0.5049623320874375
1103	60-61	0.5273609128056194
1103	62-63	0.54837323216546
1103	64-65	0.570476703133906
1103	66-67	0.5564198040659178
1103	68-69	0.5580593182513951
1103	70-71	0.5629544615348436
1103	72-73	0.5636473174578427
1103	74-75	0.5948106313723116
1103	76-77	0.49730385082871464
1103	78-79	0.5612023658947471
1103	80-81	0.48305949343732024
1103	82-83	0.4852976890985268
1103	84-85	0.5029397907868862
1103	86-87	0.529835295038346
1103	88-89	0.5758410869090298
1103	90-91	0.540127566941635
1103	92-93	0.5757970100277916
1103	94-95	0.612908827574671
1103	96-97	0.6295533302049492
1103	98-99	0.6360814592169426
1103	100-101	0.6648818481627252
1104	1	0.4767321233627797
1104	2	0.5404219634061143
1104	3	0.5599032338221157
1104	4	0.6166643851135447
1104	5	0.5530271143901473
1104	6	0.5670479167121201
1104	7	0.5655163154422667
1104	8	0.7458408073962062
1104	9	0.6989285999582435
1104	10-11	0.8738921946952658
1104	12-13	0.7731800239085445
1104	14-15	0.8977779737695002
1104	16-17	0.8582340220151963
1104	18-19	0.8684336299201689
1104	20-21	0.8983241633430694
1104	22-23	0.8233064764510019
1104	24-25	0.8569618686520499
1104	26-27	0.8760960582261248
1104	28-29	0.9603492331043171
1104	30-31	0.9127201493214656
1104	32-33	0.8969893760330621
1104	34-35	0.8873178798476502
1104	36-37	0.8901939559095595
1104	38-39	0.8349883037124641
1104	40-41	0.886633698602104
1104	42-43	0.8181362966221144
1104	44-45	0.7604459284784042
1104	46-47	0.7553385582950654
1104	48-49	0.6219591349898153
1104	50-51	0.6005268326970352
1104	52-53	0.5859803637202532
1104	54-55	0.5030843661043818
1104	56-57	0.49109173892619395
1104	58-59	0.5124530585099549
1104	60-61	0.4845166014183988
1104	62-63	0.5329129309884877
1104	64-65	0.565141153270794
1104	66-67	0.5569131304405559
1104	68-69	0.5950407832191829
1104	70-71	0.5496747835627644
1104	72-73	0.5288400210612068
1104	74-75	0.5008190626970297
1104	76-77	0.47174967371504906
1104	78-79	0.49566749916442276
1104	80-81	0.4980254551613754
1104	82-83	0.467451827958012
1104	84-85	0.4373963962457559
1104	86-87	0.46240127842945355
1104	88-89	0.5745189000698865
1104	90-91	0.539587083269339
1104	92-93	0.5624486778354765
1104	94-95	0.5939376160020444
1104	96-97	0.569126250258428
1104	98-99	0.6421896714850668
1104	100-101	0.6083807243853414
1105	1	0.36881383537825485
1105	2	0.39641947505163344
1105	3	0.3613929383550385
1105	4	0.39999800141315944
1105	5	0.41088151636058967
1105	6	0.4158185180844498
1105	7	0.4350532900634434
1105	8	0.5735838564266373
1105	9	0.5417706083698519
1105	10-11	0.7116622328796822
1105	12-13	0.5923246384541798
1105	14-15	0.6290488545254931
1105	16-17	0.5572345535303072
1105	18-19	0.6279092173745653
1105	20-21	0.5950790234346996
1105	22-23	0.5752771058624973
1105	24-25	0.5924999295237114
1105	26-27	0.639063619627322
1105	28-29	0.660286164413435
1105	30-31	0.6213316974110512
1105	32-33	0.5947536363617516
1105	34-35	0.6593786295266142
1105	36-37	0.6024152550706674
1105	38-39	0.5786444850574668
1105	40-41	0.634692033764999
1105	42-43	0.5845407810620458
1105	44-45	0.5482101045244363
1105	46-47	0.47979470013651593
1105	48-49	0.37051228466807373
1105	50-51	0.28627374575956566
1105	52-53	0.2612710411374053
1105	54-55	0.20594465486649227
1105	56-57	0.25262758809453345
1105	58-59	0.23802414822622353
1105	60-61	0.22744373774642668
1105	62-63	0.21496287135547476
1105	64-65	0.25176999815409573
1105	66-67	0.28367702626496083
1105	68-69	0.2952389786326819
1105	70-71	0.28871256655667565
1105	72-73	0.23158271486572346
1105	74-75	0.26247780181433455
1105	76-77	0.23199788056500736
1105	78-79	0.21544874655412016
1105	80-81	0.269498351497127
1105	82-83	0.22585241071795892
1105	84-85	0.2258367888397359
1105	86-87	0.20533471774033885
1105	88-89	0.1980773538478786
1105	90-91	0.24449031572313018
1105	92-93	0.23344627097042547
1105	94-95	0.30610670606239054
1105	96-97	0.29148570910350813
1105	98-99	0.2932441167967106
1105	100-101	0.32119864520107555
1106	1	0.25978478174002717
1106	2	0.3162273497523991
1106	3	0.25178145120669626
1106	4	0.36336021477182356
1106	5	0.3519610331911025
1106	6	0.32714112258427264
1106	7	0.384408499645005
1106	8	0.5324398251246549
1106	9	0.47388857855739985
1106	10-11	0.5868485271765351
1106	12-13	0.501194202978553
1106	14-15	0.5085114420628187
1106	16-17	0.5198004648359102
1106	18-19	0.5176593962166223
1106	20-21	0.5284313205308493
1106	22-23	0.47638276122544454
1106	24-25	0.4618449763084129
1106	26-27	0.5129657374612151
1106	28-29	0.5872869111644121
1106	30-31	0.5860763249664274
1106	32-33	0.5688702437260531
1106	34-35	0.5797208674168459
1106	36-37	0.6032526306756836
1106	38-39	0.5663396419926272
1106	40-41	0.6146898496877853
1106	42-43	0.4475520194463769
1106	44-45	0.45980480773872046
1106	46-47	0.4098753004312101
1106	48-49	0.2691096662841872
1106	50-51	0.3985852026120966
1106	52-53	0.2830341897946482
1106	54-55	0.2839524238613933
1106	56-57	0.2627412757789642
1106	58-59	0.25143272864782773
1106	60-61	0.280459700131928
1106	62-63	0.2703665459917275
1106	64-65	0.29698150113025434
1106	66-67	0.217835750378093
1106	68-69	0.2216507563876462
1106	70-71	0.2642513902346444
1106	72-73	0.2690402553333655
1106	74-75	0.25478669005809707
1106	76-77	0.25964680021662545
1106	78-79	0.2794029687237689
1106	80-81	0.2985175020523485
1106	82-83	0.22660614295719483
1106	84-85	0.26112601324067697
1106	86-87	0.2978233054872703
1106	88-89	0.3224873169240112
1106	90-91	0.3472853220909258
1106	92-93	0.2884856445982962
1106	94-95	0.35862401785129094
1106	96-97	0.3398981504234868
1106	98-99	0.3847791709490629
1106	100-101	0.37715073858543136
1107	1	0.14070730420010236
1107	2	0.14191530797067387
1107	3	0.19292007752693152
1107	4	0.226688401054858
1107	5	0.15576870116929342
1107	6	0.12092573594038214
1107	7	0.1202420655372265
1107	8	0.2974153198357641
1107	9	0.16481860641005142
1107	10-11	0.3668593270930316
1107	12-13	0.1900384260365584
1107	14-15	0.23947613766690523
1107	16-17	0.21173779360120193
1107	18-19	0.33161448042417874
1107	20-21	0.3033639096817282
1107	22-23	0.19742963644866052
1107	24-25	0.21174517597802378
1107	26-27	0.24040531271016619
1107	28-29	0.307878559571769
1107	30-31	0.2804884028727841
1107	32-33	0.25815143565956333
1107	34-35	0.20377853171870441
1107	36-37	0.2270350093078548
1107	38-39	0.2192797265226858
1107	40-41	0.30976859724884065
1107	42-43	0.1742297678933653
1107	44-45	0.12796065203121998
1107	46-47	0.0616479560655705
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2216	98-99	-1.4307114808170809
2216	100-101	-1.544961573126887
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	157660.0
3	31552.0
4	18982.0
5	12992.0
6	11622.0
7	12774.0
8	15001.0
9	17106.0
10	18899.0
11	21127.0
12	23570.0
13	29141.0
14	39102.0
15	58287.0
16	67018.0
17	97687.0
18	144761.0
19	71199.0
20	45513.0
21	42129.0
22	45530.0
23	49561.0
24	55565.0
25	64615.0
26	75876.0
27	91797.0
28	111471.0
29	127029.0
30	143792.0
31	174540.0
32	218103.0
33	275941.0
34	370179.0
35	548202.0
36	933233.0
37	1569168.0
38	1651540.0
39	391572.0
40	477.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.187185178441393	41.9551436299442	14.477683350019966	20.37998784159444
2	21.214086478533627	26.490679342828532	35.53519065328892	16.760043525348916
3	21.339201944932153	33.245781759824936	21.94764451660383	23.467371778639077
4	22.81642027786225	25.142131176414317	30.49434559757677	21.54710294814666
5	22.005625923951047	31.40214500324081	23.389488270810652	23.20274080199749
6	21.107581620845423	24.138623943067884	30.931624573218496	23.822169862868197
7	23.922592867049758	29.383588355101615	23.088843060925022	23.604975716923605
8	26.099358576643734	23.884900737935588	28.77634745628992	21.239393229130762
9	21.233809362055645	30.562534952834337	24.968919821628692	23.23473586348133
10-11	25.837522175613692	25.726794907400997	26.377728518849324	22.057954398135987
12-13	24.33793824120805	26.066280315434287	25.529775473001102	24.06600597035656
14-15	24.22624099753811	26.04981779806719	25.48758301918455	24.236358185210154
16-17	24.390911023855715	27.358048499876453	25.244362955195797	23.00667752107203
18-19	23.051477093616374	26.02923802503344	26.668345061243105	24.25093982010708
20-21	22.97421642662082	28.66365114334532	25.862886140642306	22.499246289391554
22-23	21.492818166393434	26.993498706520537	28.749108390936566	22.764574736149466
24-25	21.379842010064213	25.51911143044776	30.303575787472948	22.797470772015078
26-27	21.409439034409896	25.397203712417593	32.129669085629125	21.063688167543386
28-29	21.45113196903083	25.400924448715717	32.21380918088615	20.934134401367306
30-31	21.528885171619876	25.455410132723866	31.98533823318408	21.030366462472177
32-33	21.5159823309208	25.30883291827942	32.15990973710472	21.015275013695057
34-35	21.62776270728383	25.378599751077807	32.12606474747176	20.8675727941666
36-37	21.467501892626007	25.405013028301838	32.03601233674884	21.091472742323315
38-39	21.614561304261308	25.299800740409843	32.08834661145376	20.99729134387509
40-41	21.959628214282432	25.62395870681985	31.081369241157113	21.335043837740603
42-43	22.305343172238686	25.588975042518165	30.637473010750753	21.468208774492396
44-45	22.40104316567891	25.95143411019995	30.21616676962633	21.43135595449481
46-47	22.517803271385233	26.460076383780066	29.41243506385926	21.609685280975437
48-49	22.804996514413116	26.636243284386985	28.332362170546908	22.226398030652987
50-51	23.18839025220542	26.662298384329297	27.6396584618679	22.509652901597384
52-53	23.325529793965234	26.890108769637845	27.26228313638053	22.52207830001639
54-55	23.50074646594034	26.654408618903915	27.076426873795533	22.768418041360217
56-57	23.323336317326614	26.464829957079022	27.449835071583912	22.761998654010448
58-59	23.43383257626709	26.691161732885778	27.10532131400878	22.76968437683835
60-61	23.493191437029555	26.653682833052827	26.91820593481367	22.934919795103944
62-63	23.480165409577715	26.70611831535607	27.162201510550098	22.65151476451612
64-65	23.473444573420494	26.84776496154993	27.02845629971239	22.650334165317183
66-67	23.50872289128319	26.79053172714435	26.861592807622653	22.83915257394981
68-69	23.49469750243315	26.798688316948997	26.95980456905696	22.74680961156089
70-71	23.485184402591525	27.163609621759715	26.732263383575138	22.618942592073623
72-73	23.412441486978615	27.163569358462485	26.624578616233357	22.799410538325542
74-75	23.34620774031079	27.172989498842977	26.83036075386879	22.650442006977446
76-77	23.398214009833925	27.413391351200893	26.631739757177858	22.55665488178732
78-79	23.394426912594515	27.450261085845657	26.510793840277863	22.64451816128197
80-81	23.3149662763417	27.43239115817291	26.657721276154	22.594921289331392
82-83	23.325765950121422	27.60460458900898	26.486552133851372	22.583077327018227
84-85	23.317734583378773	27.474860616289398	26.49194014922594	22.715464651105886
86-87	23.322100061974762	27.33676329306272	26.679604786704274	22.661531858258243
88-89	23.413899239012867	27.42042047161829	26.455044597535395	22.710635691833446
90-91	23.395142763373812	27.305961467489233	26.412539475582925	22.886356293554037
92-93	23.43957008582156	27.22369694347394	26.619841207668056	22.71689176303644
94-95	23.537182602970866	27.390046609970113	26.41866028081921	22.654110506239807
96-97	23.533363676010435	27.320871639686306	26.23759465354283	22.908170030760427
98-99	23.545991453005637	27.219429416435453	26.448888674957967	22.785690455600943
100-101	23.73644680812081	27.330147362483316	26.042435732361852	22.890970097034028
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	361.0
1	354.0
2	366.5
3	387.5
4	398.5
5	406.5
6	421.5
7	419.5
8	423.5
9	448.0
10	472.0
11	476.5
12	475.0
13	517.5
14	588.0
15	697.5
16	1119.0
17	1558.5
18	1863.0
19	2626.0
20	4301.0
21	6792.0
22	9597.5
23	13497.5
24	19486.0
25	27043.0
26	36341.0
27	48164.0
28	61578.0
29	76268.5
30	92964.5
31	111591.0
32	132527.0
33	152807.0
34	168173.5
35	184704.5
36	207643.5
37	233713.5
38	252424.5
39	262196.5
40	269220.0
41	279236.5
42	295061.0
43	308682.0
44	315151.0
45	314751.5
46	312862.0
47	310017.5
48	307701.0
49	301165.0
50	289206.0
51	276877.0
52	252231.0
53	221517.0
54	199755.0
55	182413.5
56	164811.5
57	147388.5
58	134864.0
59	124675.5
60	110532.0
61	96853.0
62	85916.5
63	75852.5
64	66013.5
65	56188.5
66	47114.0
67	39024.5
68	31319.5
69	24830.5
70	19431.0
71	14646.0
72	11122.0
73	8609.5
74	6718.0
75	5007.0
76	3594.0
77	2679.0
78	1872.5
79	1259.5
80	757.5
81	394.5
82	250.0
83	179.5
84	117.5
85	87.5
86	70.5
87	58.0
88	46.0
89	34.5
90	25.5
91	15.5
92	12.0
93	11.0
94	9.5
95	9.5
96	8.0
97	4.5
98	4.5
99	7.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03438718876817916
2	0.05607383825486676
3	0.04467526380424167
4	0.06063071516289941
5	0.057286452558124756
6	0.016146916774961634
7	0.008526593206066696
8	0.014155676445401147
9	0.006918283709113997
10-11	0.014072708098336125
12-13	0.014200351709205389
14-15	0.014149294264857686
16-17	0.015846954289418867
18-19	0.01826580071539138
20-21	0.017863723341153208
22-23	0.019963460739952567
24-25	0.017984984771479005
26-27	0.01183256272758058
28-29	0.014091854639966518
30-31	0.0077224384575903466
32-33	0.015240647137789875
34-35	0.01201764596334101
36-37	0.024782007050267202
38-39	0.03279802581285685
40-41	0.03452759674013535
42-43	0.037705922650779974
44-45	0.03531898712752478
46-47	0.034163812449157956
48-49	0.044598677637720116
50-51	0.03649969052806545
52-53	0.03877174680153831
54-55	0.05211688631791964
56-57	0.04650056743967212
58-59	0.04794932242303824
60-61	0.04858115829684109
62-63	0.05173395548531185
64-65	0.048919413865644634
66-67	0.03857389920469095
68-69	0.030513205178297066
70-71	0.023716182899508866
72-73	0.026575399782980336
74-75	0.030206860512210835
76-77	0.02863046191797545
78-79	0.034380806587635704
80-81	0.034706297795352316
82-83	0.04821099182532023
84-85	0.048134405658798673
86-87	0.04730472218814847
88-89	0.04967251116977328
90-91	0.04496884410924098
92-93	0.03688262136067323
94-95	0.03343624386720316
96-97	0.03394681831068021
98-99	0.026626457227328034
100-101	0.027615695211564817
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	7834313.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.92346882814471
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.55784061418976	42.63400366607722
2	11.37058906296787	12.035420316632466
3	3.270406732796101	5.192438063354672
4	1.3101823319492185	2.773575752164017
5	0.6977493484182713	1.8463657945436336
6	0.4426720731327543	1.4056644998118912
7	0.31296640743906734	1.159428753585054
8	0.23494191964186203	0.9947153088472451
9	0.1741168838822309	0.8293382528935446
>10	1.384379802447779	14.46079493787081
>50	0.1493412336488875	5.5516064564787655
>100	0.0924395355505524	7.885591197401879
>500	0.0011014393610021345	0.4148452542684268
>1k	0.0011013010773727237	1.176301263152662
>5k	7.34200702365165E-5	0.3546168964624896
>10k+	9.789342698202201E-5	1.2852935864552648
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	62307	0.7953090462431103	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG	12590	0.16070330608440075	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC	12566	0.16039696141831453	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA	11771	0.15024929435420822	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC	9647	0.12313779140557697	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA	9191	0.11731724274993863	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG	8541	0.10902040804343661	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2764361086926193E-5	0.0	0.005271681128900517	0.0	0.0
2	1.2764361086926193E-5	0.0	0.00599924971085531	0.0	0.0
3	1.2764361086926193E-5	0.0	0.006573645959766989	0.0	0.0
4	1.2764361086926193E-5	0.0	0.007084220403244037	0.0	0.0
5	2.5528722173852386E-5	0.0	0.007569266124547232	1.2764361086926193E-5	0.0
6	3.8293083260778576E-5	0.0	0.008118133651285058	1.2764361086926193E-5	0.0
7	6.382180543463096E-5	0.0	0.008705294261283664	1.2764361086926193E-5	0.0
8	8.935052760848334E-5	0.0	0.009509449009760014	2.5528722173852386E-5	0.0
9	0.033136281381660394	0.0	0.010415718646931773	2.5528722173852386E-5	0.0
10-11	0.12286335764220807	0.0	0.01263671747605693	2.5528722173852386E-5	0.0
12-13	0.2554340118910235	0.0	0.01654899414919981	2.5528722173852386E-5	0.0
14-15	0.2935420119160417	1.914654163038929E-5	0.02298861431755407	2.5528722173852386E-5	0.0
16-17	0.3068488583491622	3.191090271731548E-5	0.03267038220198759	2.5528722173852386E-5	0.0
18-19	0.33002893808302014	3.8293083260778576E-5	0.04678776556412796	2.5528722173852386E-5	0.0
20-21	0.38624956649038655	3.8293083260778576E-5	0.07034439395005024	3.8293083260778576E-5	0.0
22-23	0.48669870606395227	3.8293083260778576E-5	0.19002942568161368	3.8293083260778576E-5	0.0
24-25	0.5753854868959154	3.8293083260778576E-5	0.24568842220115533	3.8293083260778576E-5	0.0
26-27	0.6242283145950385	3.8293083260778576E-5	0.25519787121091536	3.8293083260778576E-5	0.0
28-29	0.660715240762017	3.8293083260778576E-5	0.26216721236437707	3.8293083260778576E-5	0.0
30-31	0.6974319254285603	3.8293083260778576E-5	0.26497537180350084	4.467526380424168E-5	0.0
32-33	0.733440188054779	3.8293083260778576E-5	0.2667815288973009	5.105744434770477E-5	0.0
34-35	0.77382024435327	4.467526380424168E-5	0.2676303589095815	5.105744434770477E-5	0.0
36-37	0.8066182701661269	5.105744434770477E-5	0.26796861447838505	5.105744434770477E-5	0.0
38-39	0.8332128164907376	5.105744434770477E-5	0.26815369771414543	6.382180543463096E-5	0.0
40-41	0.857280019320137	5.105744434770477E-5	0.26833239876936243	6.382180543463096E-5	0.0
42-43	0.8814748657604056	5.105744434770477E-5	0.2684281314775144	6.382180543463096E-5	0.0
44-45	0.908879949014036	5.105744434770477E-5	0.2684855711024055	6.382180543463096E-5	0.0
46-47	0.938716643054726	5.105744434770477E-5	0.26849833546349244	6.382180543463096E-5	0.0
48-49	0.9677428001663961	5.105744434770477E-5	0.2685302463662098	6.382180543463096E-5	0.0
50-51	0.9951415012394833	5.105744434770477E-5	0.26858130381055745	6.382180543463096E-5	0.0
52-53	1.0195661061793166	5.105744434770477E-5	0.26858768599110094	6.382180543463096E-5	0.0
54-55	1.0470733043216425	5.105744434770477E-5	0.2686259790743617	6.382180543463096E-5	0.0
56-57	1.0770376419732017	5.105744434770477E-5	0.2686259790743617	6.382180543463096E-5	0.0
58-59	1.1104866502014918	5.105744434770477E-5	0.26863874343544863	6.382180543463096E-5	0.0
60-61	1.1480776936024895	5.105744434770477E-5	0.2686451256159921	6.382180543463096E-5	0.0
62-63	1.184468887061316	5.105744434770477E-5	0.26865150779653557	6.382180543463096E-5	0.0
64-65	1.2233810418348106	5.105744434770477E-5	0.2686642721576225	6.382180543463096E-5	0.0
66-67	1.2654396116162323	5.105744434770477E-5	0.2686642721576225	6.382180543463096E-5	0.0
68-69	1.3121635553749256	5.105744434770477E-5	0.26867703651870944	6.382180543463096E-5	0.0
70-71	1.3633039680696954	5.105744434770477E-5	0.26867703651870944	7.020398597809406E-5	0.0
72-73	1.4173865659950016	5.105744434770477E-5	0.26867703651870944	7.658616652155715E-5	0.0
74-75	1.4730647090561737	5.105744434770477E-5	0.26867703651870944	8.935052760848334E-5	0.0
76-77	1.5332295250394004	5.105744434770477E-5	0.26867703651870944	9.573270815194644E-5	0.0
78-79	1.6000637196905458	5.105744434770477E-5	0.26867703651870944	1.0849706923887264E-4	0.0
80-81	1.6719845122348316	5.105744434770477E-5	0.26867703651870944	1.467901524996512E-4	0.0
82-83	1.7493365404216044	5.743962489116787E-5	0.26867703651870944	1.531723330431143E-4	0.0
84-85	1.831858134848582	6.382180543463096E-5	0.26867703651870944	1.531723330431143E-4	0.0
86-87	1.9181898910600075	7.020398597809406E-5	0.26867703651870944	1.531723330431143E-4	0.0
88-89	2.0128440107001087	7.658616652155715E-5	0.26867703651870944	1.659366941300405E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACG	22545	0.0	85.573006	1
TATCTCG	30510	0.0	81.720276	2
TCGACGG	24360	0.0	79.04113	2
ATATCTC	32810	0.0	76.411644	1
ATCTCGA	33275	0.0	74.81545	3
CGACGGC	25955	0.0	74.312004	3
TCTCGAG	32045	0.0	73.41743	4
GACGGCG	26535	0.0	72.43792	4
ACGGCGC	26600	0.0	71.995705	5
CGGCGCG	27325	0.0	69.821045	6
CTCGAGG	33620	0.0	69.4436	5
TCGAGGG	33870	0.0	68.75295	6
AGGGCGC	35455	0.0	65.808716	9
CGAGGGC	37620	0.0	61.89524	7
GAGGGCG	38395	0.0	60.73286	8
CTCGACG	2245	0.0	55.4585	5
TCTCGAC	2515	0.0	50.069633	4
CGCGCCG	62555	0.0	33.2071	9
GGCGCGC	62625	0.0	32.896976	7
GGGCGCG	35575	0.0	32.894543	10-11
>>END_MODULE