FastQCFastQC Report
Thu 26 May 2016
SRR2048269_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048269_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7834313
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT456400.5825654400073114No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT408950.5219985466498467No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195370.24937732255527703No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT83550.10664623688126833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG32650.076.3132251
TCGACGG48100.057.8759882
ATCTCGA272200.045.453271
TCTCGAG279850.042.455532
CGACGGC66550.041.7508163
GACGGCG73500.037.8029484
ACGGCGC74300.037.523775
CTCGAGG342950.034.8031733
TCGAGGG350050.034.1379744
TATCTCG252500.032.84952
CGGCGCG86100.032.3811386
ATATCTC259500.032.1829531
GGTACTA17400.030.2991818
CGAGGGC402400.029.7086185
AGGGCGC413950.028.8337947
GGGCGCG421400.028.3691188
GAGGGCG422300.028.34246
CTCGACG19300.027.316363
TCTCGAC20100.026.7071952
CATATAT475550.026.362652
Produced by FastQC (version 0.11.5)