##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048269_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7834313 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23365673544062 34.0 31.0 34.0 30.0 34.0 2 32.39124872851008 34.0 31.0 34.0 30.0 34.0 3 32.46183539003356 34.0 31.0 34.0 30.0 34.0 4 35.92800913111335 37.0 35.0 37.0 35.0 37.0 5 35.89856532410691 37.0 35.0 37.0 35.0 37.0 6 35.829098862912424 37.0 35.0 37.0 35.0 37.0 7 35.76756213339957 37.0 35.0 37.0 33.0 37.0 8 35.71855924061242 37.0 35.0 37.0 33.0 37.0 9 37.34763520426105 39.0 37.0 39.0 34.0 39.0 10-11 37.330402870041056 39.0 37.0 39.0 34.0 39.0 12-13 37.34847343985363 39.0 37.0 39.0 34.0 39.0 14-15 38.742839352984745 40.0 38.0 41.0 34.0 41.0 16-17 38.79067066888954 40.0 38.0 41.0 34.5 41.0 18-19 38.87701480653122 40.0 38.0 41.0 35.0 41.0 20-21 38.95743602534134 40.0 38.0 41.0 35.0 41.0 22-23 38.97682521747599 40.0 38.0 41.0 35.5 41.0 24-25 38.989364734342374 40.0 38.0 41.0 36.0 41.0 26-27 38.88387858641849 40.0 38.0 41.0 35.0 41.0 28-29 38.72911919653963 40.0 38.0 41.0 35.0 41.0 30-31 38.59051182918017 40.0 38.0 41.0 35.0 41.0 32-33 38.46855352856083 40.0 38.0 41.0 35.0 41.0 34-35 38.39971583979348 40.0 38.0 41.0 34.5 41.0 36-37 38.325074387505325 40.0 38.0 41.0 34.0 41.0 38-39 38.2160517456987 40.0 38.0 41.0 34.0 41.0 40-41 38.06002715234891 40.0 37.0 41.0 34.0 41.0 42-43 37.85640731995262 40.0 37.0 41.0 33.0 41.0 44-45 37.59526757483394 40.0 37.0 41.0 33.0 41.0 46-47 37.25444183810374 40.0 37.0 41.0 32.5 41.0 48-49 36.93096229369442 40.0 36.5 41.0 31.5 41.0 50-51 36.65442917585754 40.0 36.0 41.0 31.0 41.0 52-53 36.437468926758484 40.0 36.0 41.0 31.0 41.0 54-55 36.20273915530309 39.0 35.0 41.0 30.0 41.0 56-57 35.94931999780964 39.0 35.0 41.0 30.0 41.0 58-59 35.70048624557124 39.0 35.0 41.0 29.5 41.0 60-61 35.437010686195464 38.5 35.0 40.0 29.0 41.0 62-63 35.13618909532974 38.0 35.0 40.0 28.0 41.0 64-65 34.81168706177555 37.0 35.0 40.0 28.0 41.0 66-67 34.49606232224829 37.0 34.0 40.0 28.0 41.0 68-69 34.149111543028724 36.0 34.0 39.0 27.0 41.0 70-71 33.82247556358803 36.0 34.0 39.0 26.5 41.0 72-73 33.465417580328996 35.0 34.0 38.5 26.0 40.0 74-75 33.12361173213273 35.0 33.5 37.5 26.0 40.0 76-77 32.81781855026727 35.0 33.0 37.0 26.0 39.0 78-79 32.51666955353967 35.0 33.0 36.5 26.0 39.0 80-81 32.23793745539653 35.0 33.0 36.0 25.0 38.5 82-83 31.9646483999299 35.0 33.0 36.0 25.0 37.0 84-85 31.68428833517374 35.0 33.0 35.0 24.0 37.0 86-87 31.486281630565436 35.0 33.0 35.0 24.0 36.5 88-89 31.321551934419773 35.0 33.0 35.0 23.5 36.0 90-91 31.147517529616188 35.0 33.0 35.0 23.0 36.0 92-93 30.919917802620343 35.0 32.5 35.0 19.5 36.0 94-95 30.746729483491407 35.0 32.0 35.0 18.5 35.5 96-97 30.59999683443845 35.0 32.0 35.0 16.5 35.0 98-99 30.442779985941332 35.0 32.0 35.0 2.0 35.0 100-101 29.5814743424216 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.48515662063039855 1101 2 0.45132767265426565 1101 3 0.41394575875273176 1101 4 0.336037448621461 1101 5 0.3337248889749773 1101 6 0.269341157323133 1101 7 0.230219196165244 1101 8 0.255584699458943 1101 9 0.3143293230049977 1101 10-11 0.3086412292410827 1101 12-13 0.3067060726830704 1101 14-15 0.4750581891309267 1101 16-17 0.45241442182815916 1101 18-19 0.5227664362109223 1101 20-21 0.5031699395939171 1101 22-23 0.5210552363380287 1101 24-25 0.5061286684572863 1101 26-27 0.5115357990898275 1101 28-29 0.5166482958580119 1101 30-31 0.5022355157007041 1101 32-33 0.5379410161209535 1101 34-35 0.5770680663543502 1101 36-37 0.5729034568902946 1101 38-39 0.5905788393136859 1101 40-41 0.5984139020573807 1101 42-43 0.6087099484977756 1101 44-45 0.5747252476517914 1101 46-47 0.5057394136271753 1101 48-49 0.5260891333352475 1101 50-51 0.5691799123393082 1101 52-53 0.5584763047847972 1101 54-55 0.5287693597353069 1101 56-57 0.5177791171985433 1101 58-59 0.5192489195852872 1101 60-61 0.5232277328690031 1101 62-63 0.5845792878718257 1101 64-65 0.5417543324795417 1101 66-67 0.500222527477078 1101 68-69 0.4713757106892018 1101 70-71 0.5142179720879128 1101 72-73 0.5105143444556006 1101 74-75 0.5477249720819302 1101 76-77 0.5140814058228926 1101 78-79 0.5445056408871167 1101 80-81 0.5186378087057761 1101 82-83 0.47401686649720176 1101 84-85 0.4901114066619634 1101 86-87 0.5370489189994707 1101 88-89 0.5228283204987925 1101 90-91 0.5196477599254834 1101 92-93 0.56170893923737 1101 94-95 0.5702082723188511 1101 96-97 0.5538332800062484 1101 98-99 0.5903725956650554 1101 100-101 0.8210343346303581 1102 1 0.44949381287491263 1102 2 0.40354600519904693 1102 3 0.37699348819116096 1102 4 0.2673157880942867 1102 5 0.2765674236256075 1102 6 0.28319886177265374 1102 7 0.22538630029623619 1102 8 0.23703204973982395 1102 9 0.2820094953632619 1102 10-11 0.28422821420667077 1102 12-13 0.2860387159032669 1102 14-15 0.4018449694868522 1102 16-17 0.4121418880796668 1102 18-19 0.4213798014423489 1102 20-21 0.39958147830291324 1102 22-23 0.44595350274650514 1102 24-25 0.4203733975023951 1102 26-27 0.4182267424462722 1102 28-29 0.42615784878065455 1102 30-31 0.441752631549825 1102 32-33 0.4437613571870429 1102 34-35 0.4888960631479691 1102 36-37 0.5175066813121205 1102 38-39 0.4725176438174543 1102 40-41 0.4670655457369648 1102 42-43 0.47376094032192384 1102 44-45 0.5032793992279778 1102 46-47 0.4333997294769958 1102 48-49 0.3747256765608711 1102 50-51 0.41466990531506553 1102 52-53 0.4017570031787514 1102 54-55 0.38694851436671485 1102 56-57 0.39053956299734693 1102 58-59 0.38924879439103677 1102 60-61 0.4050910038280193 1102 62-63 0.45067292693044436 1102 64-65 0.3850950975744567 1102 66-67 0.3515866514486419 1102 68-69 0.3626751400375383 1102 70-71 0.34659796688927713 1102 72-73 0.33678172317709 1102 74-75 0.34235929083043715 1102 76-77 0.34319043800191906 1102 78-79 0.35421613767957894 1102 80-81 0.30175534213313426 1102 82-83 0.3069913681068286 1102 84-85 0.3227565602188598 1102 86-87 0.3235984190171557 1102 88-89 0.313832522675348 1102 90-91 0.30603056015831953 1102 92-93 0.33793869476656013 1102 94-95 0.3206847804718791 1102 96-97 0.3086728217847039 1102 98-99 0.3394141560125732 1102 100-101 0.46780255136193105 1103 1 0.3953639940934224 1103 2 0.35990075274501976 1103 3 0.3467512362542209 1103 4 0.26387141373066925 1103 5 0.2925242641093888 1103 6 0.24008745728638559 1103 7 0.23173046263123354 1103 8 0.23255631036133195 1103 9 0.2947089628335533 1103 10-11 0.31421534094746306 1103 12-13 0.3135269932954472 1103 14-15 0.41367845266539405 1103 16-17 0.39430650625782704 1103 18-19 0.409159420468022 1103 20-21 0.4277832858106976 1103 22-23 0.4353018932620074 1103 24-25 0.43433272928267996 1103 26-27 0.4180144136907842 1103 28-29 0.45896820167143204 1103 30-31 0.4195079627754481 1103 32-33 0.4635714588885449 1103 34-35 0.45460268890594335 1103 36-37 0.48271686835848726 1103 38-39 0.4735810219178589 1103 40-41 0.5116227195755911 1103 42-43 0.42796038273440473 1103 44-45 0.4707628613129273 1103 46-47 0.46005364925937897 1103 48-49 0.4376559711830694 1103 50-51 0.4861533550350998 1103 52-53 0.46747572067227594 1103 54-55 0.4732044462117386 1103 56-57 0.45905193328941607 1103 58-59 0.40903186581569884 1103 60-61 0.39785853565614815 1103 62-63 0.49331280674851996 1103 64-65 0.4590009201899363 1103 66-67 0.4649064143489028 1103 68-69 0.4575470130656143 1103 70-71 0.43395008069960994 1103 72-73 0.3644341816441212 1103 74-75 0.3291976847039635 1103 76-77 0.3745056966767706 1103 78-79 0.37241690450373 1103 80-81 0.34135276569844564 1103 82-83 0.37409262405409294 1103 84-85 0.35284672191788147 1103 86-87 0.3626455365189436 1103 88-89 0.380601336770205 1103 90-91 0.36054232987142854 1103 92-93 0.3751992035257956 1103 94-95 0.4048848162384715 1103 96-97 0.46496906693327844 1103 98-99 0.4243838088206182 1103 100-101 0.5676902436169122 1104 1 0.3518425788027244 1104 2 0.35969012650457444 1104 3 0.32346194310065357 1104 4 0.23865002214429154 1104 5 0.221690335432271 1104 6 0.17247832347182168 1104 7 0.2125207544790797 1104 8 0.18316146048709214 1104 9 0.1871984630039023 1104 10-11 0.20839145401335912 1104 12-13 0.19650680890340766 1104 14-15 0.32624549718119056 1104 16-17 0.342809754062813 1104 18-19 0.3290717398683327 1104 20-21 0.3617923756961474 1104 22-23 0.3721825086384172 1104 24-25 0.38136865930491126 1104 26-27 0.33243271128310425 1104 28-29 0.36567015133245206 1104 30-31 0.35264864794687156 1104 32-33 0.3192311309635727 1104 34-35 0.39942506854200843 1104 36-37 0.3913338180847816 1104 38-39 0.3474597355539757 1104 40-41 0.36568875967770964 1104 42-43 0.36503729591926515 1104 44-45 0.34736788827914467 1104 46-47 0.3840503027326889 1104 48-49 0.31739694134495267 1104 50-51 0.3113582441320517 1104 52-53 0.27714846980106955 1104 54-55 0.26549381084503665 1104 56-57 0.29436860458845615 1104 58-59 0.3173909165899076 1104 60-61 0.3156494819696576 1104 62-63 0.2878136229433679 1104 64-65 0.333835842931947 1104 66-67 0.30118797644995254 1104 68-69 0.2957797169063028 1104 70-71 0.33298106577135655 1104 72-73 0.31243594287856524 1104 74-75 0.2642420038008524 1104 76-77 0.2350760712022364 1104 78-79 0.2553048591321172 1104 80-81 0.25374113227353234 1104 82-83 0.31113218158048284 1104 84-85 0.2724817998378306 1104 86-87 0.2668818502866763 1104 88-89 0.3074090003371879 1104 90-91 0.2690922027018914 1104 92-93 0.32794223948679857 1104 94-95 0.37390094565006393 1104 96-97 0.38762640955236094 1104 98-99 0.46676239487299753 1104 100-101 0.6437596796087064 1105 1 0.2917699300636798 1105 2 0.24499524540348006 1105 3 0.20304912711323908 1105 4 0.15252038078984498 1105 5 0.16902761421427215 1105 6 0.13213121784162496 1105 7 0.1833854530460357 1105 8 0.15449073382506384 1105 9 0.15691929769954527 1105 10-11 0.15006267910896298 1105 12-13 0.18466843477471429 1105 14-15 0.24964614807164764 1105 16-17 0.2971791335843079 1105 18-19 0.305162570636206 1105 20-21 0.28833996383607285 1105 22-23 0.3275933862460718 1105 24-25 0.31283995791344665 1105 26-27 0.33142926183910504 1105 28-29 0.3417649654883306 1105 30-31 0.3066793180428462 1105 32-33 0.3203254453742801 1105 34-35 0.31269654666046875 1105 36-37 0.34029804368999095 1105 38-39 0.33792932186431557 1105 40-41 0.32961625443778786 1105 42-43 0.3352669598551472 1105 44-45 0.23056957185322347 1105 46-47 0.23796791504717163 1105 48-49 0.2907062257018538 1105 50-51 0.32163953860587924 1105 52-53 0.34719844893322005 1105 54-55 0.3140374295388142 1105 56-57 0.3309500362244222 1105 58-59 0.3398612083485446 1105 60-61 0.34115390094357423 1105 62-63 0.3330728532124141 1105 64-65 0.3516997229369494 1105 66-67 0.3107650127815802 1105 68-69 0.31986473814565386 1105 70-71 0.28556500091810477 1105 72-73 0.25075828023932445 1105 74-75 0.24293895402912113 1105 76-77 0.26572202395978906 1105 78-79 0.23302029283939873 1105 80-81 0.3017445768354463 1105 82-83 0.28068225280419057 1105 84-85 0.26380180305466183 1105 86-87 0.31718861609792626 1105 88-89 0.2845199063362003 1105 90-91 0.3118870395412259 1105 92-93 0.2786840155575838 1105 94-95 0.25756982720191246 1105 96-97 0.24707440961590166 1105 98-99 0.29606319150444804 1105 100-101 0.4176361619846567 1106 1 0.24469592602017798 1106 2 0.24750839169229266 1106 3 0.1947491375498558 1106 4 0.12544984001118564 1106 5 0.15479081407003292 1106 6 0.13972758443397737 1106 7 0.11382832315420188 1106 8 0.09500323717091419 1106 9 0.10254015544771988 1106 10-11 0.06291494780714402 1106 12-13 0.13725231781373992 1106 14-15 0.11218224577752522 1106 16-17 0.15643740601706924 1106 18-19 0.19343969514707737 1106 20-21 0.1801708886606619 1106 22-23 0.2211786471329802 1106 24-25 0.19268728245065603 1106 26-27 0.20801465404043995 1106 28-29 0.18232322808918155 1106 30-31 0.1909237841647453 1106 32-33 0.12127153487210762 1106 34-35 0.1186484501048426 1106 36-37 0.18255350918195035 1106 38-39 0.18147132450186376 1106 40-41 0.1675538802255474 1106 42-43 0.15367384422900443 1106 44-45 0.07866594761696888 1106 46-47 0.07550046475170547 1106 48-49 0.08155524532547531 1106 50-51 0.1484390257276118 1106 52-53 0.14085055628940069 1106 54-55 0.10530732902738293 1106 56-57 0.1594446446501223 1106 58-59 0.17182267109231475 1106 60-61 0.13343521920519663 1106 62-63 0.18575830685040984 1106 64-65 0.15962165673621342 1106 66-67 0.15724269288782722 1106 68-69 0.09628548649460811 1106 70-71 0.10913265925289295 1106 72-73 0.07461592265745054 1106 74-75 0.13824201272800707 1106 76-77 0.05530399660572982 1106 78-79 0.14466709775353337 1106 80-81 0.1719896367795002 1106 82-83 0.1871444714858299 1106 84-85 0.23693813792656826 1106 86-87 0.2836838313082204 1106 88-89 0.26096067358395203 1106 90-91 0.28075201185953347 1106 92-93 0.26532934320381685 1106 94-95 0.23003150688814955 1106 96-97 0.22606169056800596 1106 98-99 0.2699073939245267 1106 100-101 0.40154816026381823 1107 1 0.2054722563423539 1107 2 0.1988764488200374 1107 3 0.1654180711591735 1107 4 0.1396299188564072 1107 5 0.10523708505135687 1107 6 0.10439179175507718 1107 7 0.032077473316782346 1107 8 0.09484249834132186 1107 9 0.026603605178223688 1107 10-11 0.12017143278996656 1107 12-13 0.05283318916449531 1107 14-15 0.03676708487252256 1107 16-17 0.12120176968340246 1107 18-19 0.10481071341894221 1107 20-21 0.10204501197410565 1107 22-23 0.12493897862762537 1107 24-25 0.12458016572286112 1107 26-27 0.0681689005083399 1107 28-29 0.08597475020405199 1107 30-31 0.111735536690027 1107 32-33 0.17639860886283287 1107 34-35 0.14264668391144397 1107 36-37 0.14951126907717338 1107 38-39 0.15810838607313826 1107 40-41 0.1293237842773678 1107 42-43 0.09000874723055574 1107 44-45 0.08095640473568722 1107 46-47 0.0482006466910363 1107 48-49 -0.07137528301659302 1107 50-51 -0.061618244544511924 1107 52-53 0.011551334115132761 1107 54-55 -0.018005690873280855 1107 56-57 -0.029776736789109748 1107 58-59 -0.04233701822050051 1107 60-61 0.015729575548419916 1107 62-63 0.02010481221842042 1107 64-65 0.10917268528858415 1107 66-67 -0.00962472243134016 1107 68-69 0.04440170401119303 1107 70-71 -0.018550446640418272 1107 72-73 -0.054913922187090236 1107 74-75 -0.07899948350511465 1107 76-77 -0.06909438514714594 1107 78-79 0.006997235762426612 1107 80-81 -0.0011842067992091643 1107 82-83 -0.006637460141604379 1107 84-85 -0.0638448241652263 1107 86-87 7.087430986096876E-4 1107 88-89 0.020103198559063173 1107 90-91 -0.009096467622043747 1107 92-93 -0.02393821373581062 1107 94-95 -0.03701313861782296 1107 96-97 -0.03477851556323941 1107 98-99 -0.01089757767965338 1107 100-101 0.05049810159331969 1108 1 0.14895554412847645 1108 2 0.12295373929134001 1108 3 0.09668057304690336 1108 4 0.07454412589284232 1108 5 0.07839632776357064 1108 6 0.05014723900410445 1108 7 0.05045077355500638 1108 8 0.07545636390602084 1108 9 0.03711003968646054 1108 10-11 0.030261669830018434 1108 12-13 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2214 90-91 -0.43340289947932575 2214 92-93 -0.380769049986899 2214 94-95 -0.3381925062802331 2214 96-97 -0.35873418482977826 2214 98-99 -0.4062157842289942 2214 100-101 -0.4738049739870256 2215 1 -0.539592777417031 2215 2 -0.5032368000075031 2215 3 -0.47230836132886367 2215 4 -0.37642986621364116 2215 5 -0.3382100602361007 2215 6 -0.35087119971286995 2215 7 -0.3074528854598313 2215 8 -0.33976040844069644 2215 9 -0.39322363907356106 2215 10-11 -0.3921754679279772 2215 12-13 -0.45596770956208843 2215 14-15 -0.5274507275150953 2215 16-17 -0.5217422818823181 2215 18-19 -0.599873038272797 2215 20-21 -0.5095951803828456 2215 22-23 -0.5227172811896565 2215 24-25 -0.5178118787014299 2215 26-27 -0.5569147343693857 2215 28-29 -0.5545783377685112 2215 30-31 -0.5675604111447612 2215 32-33 -0.5694675752359757 2215 34-35 -0.6575777398747036 2215 36-37 -0.651140635649206 2215 38-39 -0.664132599973172 2215 40-41 -0.5762717007733258 2215 42-43 -0.6010080200824603 2215 44-45 -0.5837559768749898 2215 46-47 -0.6491474781940596 2215 48-49 -0.6392796338921585 2215 50-51 -0.6301656857070697 2215 52-53 -0.6387787751744654 2215 54-55 -0.5821390784612319 2215 56-57 -0.6523997587488921 2215 58-59 -0.6144026869923849 2215 60-61 -0.6602336552737498 2215 62-63 -0.5765215818667073 2215 64-65 -0.6081032212397588 2215 66-67 -0.5831778475211564 2215 68-69 -0.48721957670639426 2215 70-71 -0.5249251772830874 2215 72-73 -0.45859078549310794 2215 74-75 -0.45373534389620573 2215 76-77 -0.44600133844325995 2215 78-79 -0.46128742833092673 2215 80-81 -0.4467040770103772 2215 82-83 -0.4650500520034768 2215 84-85 -0.44511791515752464 2215 86-87 -0.4748678781317466 2215 88-89 -0.5196658243896835 2215 90-91 -0.4921857005464574 2215 92-93 -0.5158061398031855 2215 94-95 -0.4989137655168321 2215 96-97 -0.5620650557121323 2215 98-99 -0.5988786089722105 2215 100-101 -0.6732637874583496 2216 1 -0.5626140413119636 2216 2 -0.578222868284211 2216 3 -0.5830264448666256 2216 4 -0.42983554872810004 2216 5 -0.47625661838597466 2216 6 -0.43108565344099503 2216 7 -0.4029618333856462 2216 8 -0.41541583150576855 2216 9 -0.5120384861511056 2216 10-11 -0.5076005418579115 2216 12-13 -0.4858801402810613 2216 14-15 -0.6850715216925423 2216 16-17 -0.6605017320617321 2216 18-19 -0.7027381706990994 2216 20-21 -0.6788428221495408 2216 22-23 -0.7210054794621499 2216 24-25 -0.6559873229839042 2216 26-27 -0.6571190491843311 2216 28-29 -0.6848288117534409 2216 30-31 -0.6426158084306692 2216 32-33 -0.6883837765456633 2216 34-35 -0.729748225390459 2216 36-37 -0.7815570911248741 2216 38-39 -0.7143637428801313 2216 40-41 -0.7335462570666991 2216 42-43 -0.6712709569056301 2216 44-45 -0.64845592356005 2216 46-47 -0.6477552171320795 2216 48-49 -0.6672535037532015 2216 50-51 -0.589262500937032 2216 52-53 -0.6070698637148482 2216 54-55 -0.619444842357666 2216 56-57 -0.5698146048104746 2216 58-59 -0.5862495350182755 2216 60-61 -0.6056307131683383 2216 62-63 -0.6454081708724644 2216 64-65 -0.6321439022207613 2216 66-67 -0.6116865584787377 2216 68-69 -0.5738224250094532 2216 70-71 -0.5496533899233569 2216 72-73 -0.5615892537500073 2216 74-75 -0.5734357778183536 2216 76-77 -0.6490859039939352 2216 78-79 -0.6148699858560178 2216 80-81 -0.6628852283068198 2216 82-83 -0.6561689676328193 2216 84-85 -0.5501148019200208 2216 86-87 -0.5362220154609432 2216 88-89 -0.5926678993255479 2216 90-91 -0.6715438801790761 2216 92-93 -0.5665889143882303 2216 94-95 -0.5920942562803262 2216 96-97 -0.6431708595647159 2216 98-99 -0.7697297415822426 2216 100-101 -0.9078617395037512 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 782.0 3 164.0 4 166.0 5 230.0 6 454.0 7 1099.0 8 2349.0 9 4064.0 10 6449.0 11 7612.0 12 8421.0 13 12398.0 14 21192.0 15 37580.0 16 53391.0 17 75918.0 18 76581.0 19 49639.0 20 38827.0 21 34049.0 22 31469.0 23 30979.0 24 32293.0 25 37266.0 26 44507.0 27 56161.0 28 69768.0 29 87401.0 30 114600.0 31 152637.0 32 203928.0 33 277248.0 34 390321.0 35 565275.0 36 961304.0 37 1785671.0 38 2033408.0 39 527671.0 40 1041.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.79222942978359 41.21862936618169 15.165686926800328 21.823454277234397 2 21.692610009296757 27.255127707224382 33.36593965048173 17.686322632997133 3 21.692916179918473 31.376814073081665 23.12829576181879 23.80197398518107 4 22.052986649882385 25.42350554541285 29.980101637501587 22.543406167203173 5 22.834819445536507 29.95197280884397 24.28006441408001 22.933143331539515 6 23.58187629215223 24.71138184037324 29.07491697102222 22.63182489645231 7 22.93431472549029 30.975747331004005 23.64505987953251 22.444878063973192 8 24.12874546111567 24.439394254637552 29.24484529404159 22.187014990205185 9 23.344446411574317 28.28627092126649 25.57355060998967 22.79573205716953 10-11 23.996877581990915 26.08824794209779 26.84512349710817 23.069750978803118 12-13 23.69177105893012 26.39726035965119 26.110611102722086 23.8003574786966 14-15 23.210542449279345 26.502466682286 26.326139696949642 23.960851171485015 16-17 23.34043840219302 26.605791726728306 26.26703834784237 23.786731523236305 18-19 22.805101098207334 26.83384618408787 27.316830461075526 23.044222256629265 20-21 21.856646642573935 26.60828247532888 28.438774940366496 23.096295941730688 22-23 21.47763500278593 26.090052278496124 29.408067776316198 23.024244942401747 24-25 21.37750827262149 25.81218736740957 30.55592501464079 22.254379345328147 26-27 21.287656270963414 25.696784704941756 31.312454610523986 21.70310441357084 28-29 21.48017614621839 25.670175391116363 31.346532023187695 21.50311643947755 30-31 21.424031634821944 25.582350500051128 31.19898533284639 21.79463253228054 32-33 21.55404385114287 25.571313089899682 31.179667249447522 21.694975809509927 34-35 21.605509359158216 25.70130981625045 31.064319042978283 21.628861781613047 36-37 21.44375007738394 25.590814408359737 30.985378041444093 21.98005747281223 38-39 21.51249254401758 25.623689020339114 31.031987105953 21.8318313296903 40-41 21.676367793832082 25.84744827019293 30.7219599216932 21.75422401428179 42-43 21.882237791622572 25.86913491967962 30.202814209746283 22.045813078951532 44-45 21.804222016659278 25.996529625507687 29.956538626935124 22.24270973089791 46-47 22.0338847835158 26.26661074174596 29.156576971075832 22.542927503662412 48-49 22.270180809262545 26.495564922761822 28.376057073597654 22.858197194377983 50-51 22.44080623278646 26.36017989069367 28.221772604694245 22.977241271825623 52-53 22.50697058660405 26.51650267670398 27.886124736786044 23.090401999905925 54-55 22.64344341420846 26.3466828591792 27.603710891913387 23.40616283469896 56-57 22.715516303249952 26.31568768831406 27.67855381948644 23.290242188949545 58-59 22.780364116196367 26.736692792588723 27.386665497373208 23.096277593841705 60-61 22.772482914740568 26.610230534445563 27.198244663072284 23.419041887741585 62-63 22.800130763723203 26.49429529854377 27.42642721570411 23.279146722028926 64-65 22.811521351634635 26.632970001875723 27.303252687394856 23.252255959094782 66-67 22.88786265672811 26.539365260417437 27.224904739026034 23.347867343828423 68-69 22.865710506539365 26.551495589101116 27.34556907695818 23.23722482740134 70-71 23.03646880910509 26.72316184061175 26.988316438072463 23.2520529122107 72-73 22.966345757408735 26.639076520659806 26.94433431475692 23.450243407174547 74-75 22.967263975949688 26.658647598925945 27.12386160043011 23.250226824694252 76-77 22.89047414088005 26.965581846792613 26.95687015144692 23.187073860880417 78-79 22.901106489657256 26.89898247882531 26.871264392758192 23.32864663875924 80-81 22.905676643747043 26.901233809906806 26.97867641436784 23.214413131978308 82-83 22.859813526689614 27.115739526057908 26.843679881761144 23.180767065491334 84-85 22.914725343515272 27.081509021440375 26.730960310972524 23.27280532407183 86-87 22.81356369037023 27.131886898995095 26.848611646811378 23.205937763823297 88-89 22.88040174256091 27.29615525399475 26.582108656989245 23.24133434645509 90-91 22.820009599011506 27.244707140796564 26.528068173405124 23.407215086786806 92-93 22.804145832356042 27.244541753665768 26.69966330030798 23.25164911367021 94-95 22.864934442945014 27.405875499036775 26.54260378867136 23.186586269346847 96-97 22.864179704326045 27.338253424769004 26.348895213259876 23.448671657645068 98-99 22.877507134932607 27.176579665494728 26.602383291189945 23.343529908382724 100-101 22.95555545627506 27.311653026590648 26.347067867648605 23.385723649485683 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 645.0 1 478.0 2 276.0 3 207.5 4 172.5 5 153.5 6 141.5 7 152.5 8 158.5 9 148.5 10 162.0 11 176.5 12 211.0 13 432.5 14 640.0 15 959.0 16 4332.5 17 5148.0 18 3188.0 19 3904.5 20 5449.5 21 7711.5 22 10422.0 23 14595.5 24 20683.0 25 28015.5 26 36973.0 27 48169.5 28 60758.0 29 74672.5 30 89877.0 31 107218.5 32 127863.5 33 148176.0 34 162536.0 35 177584.5 36 196954.5 37 223371.0 38 248318.0 39 264357.0 40 273941.0 41 282707.0 42 299374.5 43 312890.0 44 317959.5 45 316987.5 46 314378.5 47 311521.5 48 309986.0 49 303229.0 50 291715.5 51 280572.0 52 254405.0 53 221901.0 54 200304.0 55 183603.5 56 165762.0 57 147513.0 58 135007.0 59 125457.0 60 111333.0 61 97969.0 62 87278.5 63 76805.0 64 66845.0 65 56724.0 66 47445.5 67 39116.5 68 31053.5 69 24518.0 70 18860.5 71 13959.0 72 10559.5 73 8105.0 74 6262.0 75 4472.5 76 3066.0 77 2242.0 78 1604.5 79 979.0 80 462.0 81 192.0 82 78.0 83 37.5 84 19.5 85 11.5 86 6.0 87 4.5 88 4.5 89 2.0 90 3.5 91 4.0 92 2.0 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29646505060494777 2 0.018827432603216135 3 1.1487924978233572E-4 4 0.0 5 1.2764361086926191E-5 6 0.0 7 0.0 8 8.169191095632762E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 5.743962489116786E-5 16-17 0.0 18-19 0.0 20-21 6.382180543463096E-6 22-23 0.003931423214773268 24-25 4.722813602162691E-4 26-27 0.009241397426934563 28-29 0.011928295435732527 30-31 0.005284445489987444 32-33 4.595169991293429E-4 34-35 1.0211488869540953E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 9.573270815194644E-5 50-51 0.0 52-53 0.0018444501770608348 54-55 0.0030570644803188233 56-57 0.006273683474224224 58-59 0.0066693786679189355 60-61 0.0018827432603216133 62-63 0.004288825325207201 64-65 8.296834706502026E-5 66-67 5.743962489116786E-4 68-69 3.254912077166179E-4 70-71 1.9784759684735598E-4 72-73 0.0014679015249965122 74-75 8.807409149979074E-4 76-77 2.1699413847774525E-4 78-79 9.95620164780243E-4 80-81 0.0015827807747788478 82-83 1.914654163038929E-5 84-85 0.003944187575860193 86-87 0.007524590860742991 88-89 0.005622701058790988 90-91 0.0022082344680382313 92-93 0.002303967176190178 94-95 0.0028209238002106884 96-97 0.0031208862857534537 98-99 0.0018699788992346872 100-101 0.0021444126626036005 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7834313.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.4997711163751 #Duplication Level Percentage of deduplicated Percentage of total 1 79.26062135414573 40.02643236926032 2 11.993324992516811 12.113203340928003 3 3.537416768596163 5.359162114720005 4 1.4244911562266778 2.8774590938699105 5 0.7615089458977119 1.922801373545326 6 0.47998733606206606 1.4543550365935773 7 0.3245369310845192 1.1472328517005315 8 0.23688979811318644 0.9570304467616179 9 0.1809534727908435 0.8224298062785728 >10 1.524216409923599 15.331037061872921 >50 0.16338731462401854 5.819805291927764 >100 0.11013000947678266 9.103100340577114 >500 0.0015974504201562015 0.5559229212127879 >1k 7.859422653812356E-4 0.8984001889115735 >5k 7.605892816785576E-5 0.2535672745874103 >10k+ 7.605892816785576E-5 1.3580604872525013 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 45640 0.5825654400073114 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 40895 0.5219985466498467 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19537 0.24937732255527703 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 8355 0.10664623688126833 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0018380679965173717 0.0 0.0 0.0 2 0.0 0.0020805908571689693 0.0 0.0 0.0 3 0.0 0.0024124642454290504 0.0 0.0 0.0 4 0.0 0.002591165300646017 0.0 0.0 0.0 5 1.2764361086926193E-5 0.0028209238002106884 0.0 0.0 0.0 6 2.5528722173852386E-5 0.0032166189939054003 0.0 0.0 0.0 7 6.382180543463096E-5 0.003637842909773965 0.0 0.0 0.0 8 8.935052760848334E-5 0.00412288863107716 0.0 0.0 0.0 9 0.03387661432470211 0.004722813602162691 0.0 0.0 0.0 10-11 0.12468227909709506 0.0066693786679189355 0.0 0.0 0.0 12-13 0.2586697774265593 0.010351896841497142 0.0 0.0 0.0 14-15 0.2968735101597294 0.01675960610713409 0.0 0.0 0.0 16-17 0.3101867387733934 0.02644137399156761 0.0 6.3821805434630965E-6 0.0 18-19 0.3330923847438825 0.04061619697859914 0.0 1.2764361086926193E-5 0.0 20-21 0.3887577634439676 0.06470892853017234 0.0 2.5528722173852386E-5 0.0 22-23 0.4885303918799262 0.18515343974640788 0.0 2.5528722173852386E-5 0.0 24-25 0.575902443519936 0.24157191575062165 0.0 2.5528722173852386E-5 0.0 26-27 0.6237943263180831 0.25170043627309757 0.0 2.5528722173852386E-5 0.0 28-29 0.6591452243483251 0.25915482314786253 0.0 2.5528722173852386E-5 0.0 30-31 0.6948088492251969 0.2625118501137241 0.0 3.191090271731548E-5 0.0 32-33 0.7303703592133732 0.2648413460120881 0.0 3.8293083260778576E-5 0.0 34-35 0.770265369790561 0.2662262791900196 0.0 5.105744434770477E-5 0.0 36-37 0.8024634706323324 0.26711340228556096 0.0 7.020398597809406E-5 0.0 38-39 0.8283878879998795 0.26782182432588536 0.0 7.658616652155715E-5 0.0 40-41 0.8515743499142809 0.2684345136580578 0.0 7.658616652155715E-5 0.0 42-43 0.8750799208558555 0.26876000486577445 0.0 7.658616652155715E-5 0.0 44-45 0.9016106453750317 0.26902167426805645 0.0 7.658616652155715E-5 0.0 46-47 0.9303049290984416 0.26912378915675184 0.0 7.658616652155715E-5 0.0 48-49 0.9572377309918559 0.26915570005946915 0.0 7.658616652155715E-5 0.0 50-51 0.9832770276091853 0.26921313968436034 0.0 7.658616652155715E-5 0.0 52-53 1.0074910205910843 0.2692259040454473 0.0 7.658616652155715E-5 0.0 54-55 1.0344748799288463 0.26927057930925147 0.0 8.935052760848334E-5 0.0 56-57 1.0638201460676897 0.26928334367033846 0.0 1.0211488869540954E-4 0.0 58-59 1.0960693043538088 0.26930249021196884 0.0 1.0211488869540954E-4 0.0 60-61 1.1325626127013306 0.2693152545730557 0.0 1.0211488869540954E-4 0.0 62-63 1.1684432317166802 0.26932801893414265 0.0 1.0211488869540954E-4 0.0 64-65 1.2071064814489796 0.2693407832952296 0.0 1.0211488869540954E-4 0.0 66-67 1.249005496716815 0.2693407832952296 0.0 1.0211488869540954E-4 0.0 68-69 1.2947593490329017 0.2693535476563165 0.0 1.1487924978233573E-4 0.0 70-71 1.3450190208126735 0.2693535476563165 0.0 1.1487924978233573E-4 0.0 72-73 1.3987250700859155 0.2693535476563165 0.0 1.2764361086926192E-4 0.0 74-75 1.454116015022632 0.2693726941979469 0.0 1.2764361086926192E-4 0.0 76-77 1.5139361932565114 0.26937907637849035 0.0 1.2764361086926192E-4 0.0 78-79 1.5802789600058103 0.26937907637849035 0.0 1.404079719561881E-4 0.0 80-81 1.6516062097595539 0.26937907637849035 0.0 1.404079719561881E-4 0.0 82-83 1.7284285169612192 0.26937907637849035 0.0 1.404079719561881E-4 0.0 84-85 1.8105735627361326 0.26937907637849035 0.0 1.404079719561881E-4 0.0 86-87 1.8964011266846243 0.26937907637849035 0.0 1.404079719561881E-4 0.0 88-89 1.9901936519513583 0.26937907637849035 0.0 1.404079719561881E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 3265 0.0 76.313225 1 TCGACGG 4810 0.0 57.875988 2 ATCTCGA 27220 0.0 45.45327 1 TCTCGAG 27985 0.0 42.45553 2 CGACGGC 6655 0.0 41.750816 3 GACGGCG 7350 0.0 37.802948 4 ACGGCGC 7430 0.0 37.52377 5 CTCGAGG 34295 0.0 34.803173 3 TCGAGGG 35005 0.0 34.137974 4 TATCTCG 25250 0.0 32.8495 2 CGGCGCG 8610 0.0 32.381138 6 ATATCTC 25950 0.0 32.182953 1 GGTACTA 1740 0.0 30.299181 8 CGAGGGC 40240 0.0 29.708618 5 AGGGCGC 41395 0.0 28.833794 7 GGGCGCG 42140 0.0 28.369118 8 GAGGGCG 42230 0.0 28.3424 6 CTCGACG 1930 0.0 27.31636 3 TCTCGAC 2010 0.0 26.707195 2 CATATAT 47555 0.0 26.36265 2 >>END_MODULE