FastQCFastQC Report
Thu 26 May 2016
SRR2048268_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048268_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5085140
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT423890.8335857026551874No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC85440.16801897292896556No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG85220.16758633980578705No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA80220.15775376882445716No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA64200.1262502114002761No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC62710.12332010524783979No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG59390.11679127811623671No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG168800.090.862521
TCGACGG176500.086.631042
TATCTCG194150.086.073052
CGACGGC187000.081.715923
GACGGCG188100.080.884394
ATCTCGA207500.080.466643
ACGGCGC188650.080.37395
ATATCTC210750.079.765281
TCTCGAG201750.079.4627764
CGGCGCG194800.077.801216
CTCGAGG208750.075.9354555
TCGAGGG210800.075.117916
AGGGCGC216450.073.062169
GAGGGCG232550.068.024978
CGAGGGC233500.067.788897
CTCGACG11350.053.1683165
TCTCGAC13600.046.117664
CGCGCCG408200.039.1603789
GGCGCGC408750.038.8408437
GCGCGCC412000.038.695838
Produced by FastQC (version 0.11.5)