##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2048268_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5085140
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.998682828791342	31.0	31.0	34.0	28.0	34.0
2	31.186182484651358	33.0	31.0	34.0	28.0	34.0
3	31.237737997380602	33.0	31.0	34.0	28.0	34.0
4	34.751078239733815	37.0	35.0	37.0	32.0	37.0
5	34.704504300766544	37.0	35.0	37.0	32.0	37.0
6	34.68562910755653	37.0	35.0	37.0	32.0	37.0
7	34.61186417679749	37.0	35.0	37.0	32.0	37.0
8	34.410998320596875	37.0	35.0	37.0	31.0	37.0
9	36.03226990800647	39.0	37.0	39.0	31.0	39.0
10-11	35.75712389432739	39.0	37.0	39.0	30.0	39.0
12-13	35.824128342582505	39.0	37.0	39.0	30.0	39.0
14-15	37.0667047514916	40.0	38.0	41.0	31.0	41.0
16-17	37.04719309596196	40.0	37.5	41.0	31.0	41.0
18-19	37.035467361763885	40.0	38.0	41.0	30.5	41.0
20-21	37.057536960634316	40.0	38.0	41.0	31.0	41.0
22-23	37.0938329131548	40.0	38.0	41.0	31.0	41.0
24-25	37.16238795785367	40.0	38.0	41.0	31.0	41.0
26-27	37.10998635239147	40.0	38.0	41.0	31.0	41.0
28-29	36.989107576192595	40.0	37.5	41.0	31.0	41.0
30-31	36.78814290265362	40.0	36.5	41.0	30.5	41.0
32-33	36.5464620443095	40.0	35.5	41.0	30.0	41.0
34-35	36.411085535501485	40.0	35.0	41.0	30.0	41.0
36-37	36.317804819532995	40.0	35.0	41.0	30.0	41.0
38-39	36.192939919058276	40.0	35.0	41.0	30.0	41.0
40-41	35.78350714041305	40.0	35.0	41.0	27.5	41.0
42-43	35.579227218916294	39.5	35.0	41.0	27.0	41.0
44-45	35.358341461592005	39.0	35.0	41.0	25.5	41.0
46-47	35.03361677357949	39.0	35.0	41.0	23.5	41.0
48-49	34.540843123296504	39.0	34.5	41.0	20.5	41.0
50-51	34.209245664819456	39.0	34.0	41.0	17.5	41.0
52-53	33.99671169328671	39.0	34.0	41.0	14.0	41.0
54-55	33.78103326555414	39.0	34.0	40.5	11.0	41.0
56-57	33.54929146493508	38.0	33.0	40.0	8.0	41.0
58-59	33.31572493579331	38.0	33.0	40.0	7.0	41.0
60-61	33.05791453136	37.5	33.0	40.0	4.0	41.0
62-63	32.772905957358105	37.0	33.0	40.0	2.0	41.0
64-65	32.480975155059646	36.5	32.5	40.0	2.0	41.0
66-67	32.169009506129626	36.0	32.0	39.0	2.0	41.0
68-69	31.81903890551686	35.5	32.0	39.0	2.0	41.0
70-71	31.483476954420134	35.0	31.5	39.0	2.0	40.5
72-73	31.134519501921282	35.0	31.0	37.5	2.0	40.0
74-75	30.787574973353735	35.0	31.0	37.0	2.0	39.5
76-77	30.47175475994761	35.0	31.0	37.0	2.0	39.0
78-79	30.16176624439052	35.0	31.0	36.0	2.0	39.0
80-81	29.850935175826034	35.0	30.0	36.0	2.0	37.5
82-83	29.62183607530963	35.0	30.0	35.0	2.0	37.0
84-85	29.403653291748114	35.0	30.0	35.0	2.0	37.0
86-87	29.160798227777406	34.0	30.0	35.0	2.0	36.0
88-89	28.944795620179583	34.0	30.0	35.0	2.0	36.0
90-91	28.74450614928989	34.0	30.0	35.0	2.0	36.0
92-93	28.5125556031889	34.0	29.5	35.0	2.0	35.5
94-95	28.25002045174764	34.0	29.0	35.0	2.0	35.0
96-97	27.97375824461077	34.0	29.0	35.0	2.0	35.0
98-99	27.666032793590738	34.0	29.0	35.0	2.0	35.0
100-101	26.427087749796463	33.0	26.0	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.7055277283629771
1101	2	0.7658593726217795
1101	3	0.7428693445714885
1101	4	0.708775710678772
1101	5	0.7233623968894705
1101	6	0.7464104186919513
1101	7	0.7793766750904396
1101	8	0.8899886520770082
1101	9	0.9312019719017641
1101	10-11	1.0833976157808678
1101	12-13	0.9895018039664123
1101	14-15	1.100227006201692
1101	16-17	1.0599173137743847
1101	18-19	1.0854761830921404
1101	20-21	1.0469739731883578
1101	22-23	1.0525164444956445
1101	24-25	1.0366512912215526
1101	26-27	1.1194030351599693
1101	28-29	1.1069222911467165
1101	30-31	1.0602635523746429
1101	32-33	1.0122197978276262
1101	34-35	1.0441680084519973
1101	36-37	1.0057908953709287
1101	38-39	1.0409163982705252
1101	40-41	1.043703215299594
1101	42-43	0.9699603841530902
1101	44-45	0.9301819007021166
1101	46-47	0.9054969831904884
1101	48-49	0.7542616290308786
1101	50-51	0.6722702482007321
1101	52-53	0.5539776223395947
1101	54-55	0.4943432783639281
1101	56-57	0.5637667332738729
1101	58-59	0.48434306674829486
1101	60-61	0.49408394636912334
1101	62-63	0.5416763682239676
1101	64-65	0.4649369118543234
1101	66-67	0.49715553434674575
1101	68-69	0.5650197375436079
1101	70-71	0.502288991655373
1101	72-73	0.451240285675933
1101	74-75	0.49574279607296745
1101	76-77	0.5220055247246087
1101	78-79	0.48969153558296696
1101	80-81	0.5675761328938762
1101	82-83	0.561419502969084
1101	84-85	0.47188953882762874
1101	86-87	0.49841101163575985
1101	88-89	0.5723032551377578
1101	90-91	0.5760355486461926
1101	92-93	0.5029507663959762
1101	94-95	0.47326723026680995
1101	96-97	0.46115633199567085
1101	98-99	0.4721607841156654
1101	100-101	0.49165875361231315
1102	1	0.5337464666062282
1102	2	0.5661592505154758
1102	3	0.5985897464312053
1102	4	0.6170246237042036
1102	5	0.602360429927856
1102	6	0.6060251373512386
1102	7	0.6653997126498794
1102	8	0.7890765619711644
1102	9	0.8099455144823864
1102	10-11	0.9434523195140798
1102	12-13	0.8685161369203556
1102	14-15	0.9631315592355918
1102	16-17	0.967458188656984
1102	18-19	0.9906672116816964
1102	20-21	0.9428697355550071
1102	22-23	0.9332112458003579
1102	24-25	0.9454013111881068
1102	26-27	1.0298827836920381
1102	28-29	1.0702189140362606
1102	30-31	0.991190885999309
1102	32-33	0.9704549420992237
1102	34-35	0.8852256055497563
1102	36-37	0.8893524541817541
1102	38-39	0.8578123162467364
1102	40-41	0.8239324788500397
1102	42-43	0.64304454648601
1102	44-45	0.6630684256013382
1102	46-47	0.6736157474167257
1102	48-49	0.5049665637689174
1102	50-51	0.38297266687165177
1102	52-53	0.2762939279798502
1102	54-55	0.1741556448203596
1102	56-57	0.1956918177354865
1102	58-59	0.1110941164369521
1102	60-61	0.17553702830691975
1102	62-63	0.17585842212732672
1102	64-65	0.1489912102411921
1102	66-67	0.11244319248549317
1102	68-69	0.12502321876399947
1102	70-71	0.10516187035991464
1102	72-73	0.05690540637765196
1102	74-75	0.06188404598203334
1102	76-77	0.03617403764490135
1102	78-79	0.029963208652485918
1102	80-81	0.08445225666716638
1102	82-83	0.10859884820271404
1102	84-85	0.1780677790689218
1102	86-87	0.15239977219626155
1102	88-89	0.21770230940020951
1102	90-91	0.19341734604046934
1102	92-93	0.15481923123658348
1102	94-95	0.29087383357325436
1102	96-97	0.22790589205002476
1102	98-99	0.29723200528438554
1102	100-101	0.23002784015896438
1103	1	0.47998604812851653
1103	2	0.5124624979908745
1103	3	0.4810419678197668
1103	4	0.5118604675848388
1103	5	0.5163146838381678
1103	6	0.5323214758602646
1103	7	0.5813347889225611
1103	8	0.7417751372575268
1103	9	0.7283248282037604
1103	10-11	0.8719138207143544
1103	12-13	0.7605119759956764
1103	14-15	0.8367733725871886
1103	16-17	0.7953714958456501
1103	18-19	0.8398353909456446
1103	20-21	0.8564852942397749
1103	22-23	0.8221496750687081
1103	24-25	0.8198259842890252
1103	26-27	0.8822652122882175
1103	28-29	0.8319569525086337
1103	30-31	0.879461640351451
1103	32-33	0.8249512162499997
1103	34-35	0.7972572772165805
1103	36-37	0.7931197202533937
1103	38-39	0.8328462266488614
1103	40-41	0.8244905115661396
1103	42-43	0.7536268599695575
1103	44-45	0.6830873966962585
1103	46-47	0.6572985756155134
1103	48-49	0.5203284489674829
1103	50-51	0.521627811317444
1103	52-53	0.4866128113716215
1103	54-55	0.41352096194933097
1103	56-57	0.3810633311883791
1103	58-59	0.4118310126809632
1103	60-61	0.29282440934762377
1103	62-63	0.2883914982145299
1103	64-65	0.2789903015466848
1103	66-67	0.2583758034544701
1103	68-69	0.3130508426523093
1103	70-71	0.27319755676181856
1103	72-73	0.303039658760234
1103	74-75	0.31046068826897155
1103	76-77	0.27446753484310094
1103	78-79	0.29402278214623934
1103	80-81	0.2973541981665626
1103	82-83	0.22649732045980997
1103	84-85	0.24949778242019605
1103	86-87	0.2660543607860433
1103	88-89	0.3092908902425826
1103	90-91	0.2928433586110373
1103	92-93	0.38743317540120614
1103	94-95	0.4088124315202748
1103	96-97	0.3281697553590952
1103	98-99	0.3531386225787223
1103	100-101	0.3856940154850186
1104	1	0.5600487834990453
1104	2	0.6193914852039768
1104	3	0.6109371178698879
1104	4	0.651621286476086
1104	5	0.6373323575118945
1104	6	0.646393364654493
1104	7	0.7168415636803047
1104	8	0.8338925362883955
1104	9	0.756679830928114
1104	10-11	0.9446079095212241
1104	12-13	0.8826537988736121
1104	14-15	0.9398039766509427
1104	16-17	0.8925024162955495
1104	18-19	0.9463152161857025
1104	20-21	0.9232548942767096
1104	22-23	0.8868622433154911
1104	24-25	0.8855858077630145
1104	26-27	0.8914007847083312
1104	28-29	0.9161725948086499
1104	30-31	0.959752729661048
1104	32-33	0.9395329352914672
1104	34-35	0.8917451641323595
1104	36-37	0.8786654587737317
1104	38-39	0.9015568401851297
1104	40-41	0.9024856187691626
1104	42-43	0.8771395590655615
1104	44-45	0.8599943382265351
1104	46-47	0.8097130244182509
1104	48-49	0.6167513588169982
1104	50-51	0.592274069923235
1104	52-53	0.5442652522009865
1104	54-55	0.47142626084041694
1104	56-57	0.5158439831666257
1104	58-59	0.5464650914734577
1104	60-61	0.4814683339407537
1104	62-63	0.5045637274775956
1104	64-65	0.49421566020294705
1104	66-67	0.48940203651256553
1104	68-69	0.5183958621040858
1104	70-71	0.5202529077818703
1104	72-73	0.4988302478525881
1104	74-75	0.49800618194877444
1104	76-77	0.507963707202812
1104	78-79	0.49095862288750425
1104	80-81	0.6039089506317765
1104	82-83	0.5148479180613741
1104	84-85	0.5185890928890338
1104	86-87	0.5553695698989287
1104	88-89	0.6067885095697321
1104	90-91	0.5285968904859644
1104	92-93	0.5699696631636932
1104	94-95	0.5740884641402602
1104	96-97	0.5968463019435184
1104	98-99	0.6248870348781459
1104	100-101	0.5436349567363337
1105	1	0.42853459426622464
1105	2	0.4636039267548213
1105	3	0.41557893992616
1105	4	0.5149916423559517
1105	5	0.4628525407507098
1105	6	0.41527117885550524
1105	7	0.5129394961660694
1105	8	0.6529043609656213
1105	9	0.6228840069801933
1105	10-11	0.6969974591351118
1105	12-13	0.6858472214206515
1105	14-15	0.6849358409843305
1105	16-17	0.6201053077007259
1105	18-19	0.6141733276559833
1105	20-21	0.5570144047282355
1105	22-23	0.5566340494561217
1105	24-25	0.6126055370831267
1105	26-27	0.6435618745690519
1105	28-29	0.6810691241348366
1105	30-31	0.6628821097866293
1105	32-33	0.6906030059052597
1105	34-35	0.6215476240298798
1105	36-37	0.6517556195635095
1105	38-39	0.6279071248814247
1105	40-41	0.6210715955300543
1105	42-43	0.5026745943724578
1105	44-45	0.45514327674048616
1105	46-47	0.46337660418449644
1105	48-49	0.3750891806909351
1105	50-51	0.2771164328834672
1105	52-53	0.2145102923319584
1105	54-55	0.13818007736610127
1105	56-57	0.19858927969519158
1105	58-59	0.2466529454445805
1105	60-61	0.22349924979296532
1105	62-63	0.13572661402070452
1105	64-65	0.2166088183755761
1105	66-67	0.1307846842830429
1105	68-69	0.23249932518093885
1105	70-71	0.2415185622794631
1105	72-73	0.21620019729478201
1105	74-75	0.27817382799094403
1105	76-77	0.23013176727961948
1105	78-79	0.2134670460233039
1105	80-81	0.26014710411327613
1105	82-83	0.18331477439895494
1105	84-85	0.21177554521997521
1105	86-87	0.21113733825225012
1105	88-89	0.2606547383979354
1105	90-91	0.2147941153143691
1105	92-93	0.23330249003264214
1105	94-95	0.28390683968106956
1105	96-97	0.2930359677909813
1105	98-99	0.28552392650363245
1105	100-101	0.2003794669789407
1106	1	0.229427804478064
1106	2	0.24415232436481915
1106	3	0.23084853978131292
1106	4	0.3168865545413553
1106	5	0.28887361625314867
1106	6	0.3392791455402744
1106	7	0.37382524666399064
1106	8	0.5113386901345507
1106	9	0.45744160248988663
1106	10-11	0.5863211962345076
1106	12-13	0.4633265261463464
1106	14-15	0.561172741219707
1106	16-17	0.46645113424427365
1106	18-19	0.5080494819199615
1106	20-21	0.4680516308734468
1106	22-23	0.4579680708213232
1106	24-25	0.4741457145229191
1106	26-27	0.4811359828435329
1106	28-29	0.4609553453913975
1106	30-31	0.47217557348389505
1106	32-33	0.49406626862480607
1106	34-35	0.438382460169656
1106	36-37	0.459844013552285
1106	38-39	0.4841638331921274
1106	40-41	0.5180865511701
1106	42-43	0.4109395590655538
1106	44-45	0.32382790466009936
1106	46-47	0.35657689388212077
1106	48-49	0.13777746604310437
1106	50-51	-0.01678094173178124
1106	52-53	0.09578086991660228
1106	54-55	0.06634980396395918
1106	56-57	0.02985004377269007
1106	58-59	0.04861847142683473
1106	60-61	0.009539662612084499
1106	62-63	-0.04329594618208432
1106	64-65	-0.013484572897283442
1106	66-67	-0.04463432712380211
1106	68-69	-0.04980833370010629
1106	70-71	0.008693467222428808
1106	72-73	0.021614863237207516
1106	74-75	0.01958193952453513
1106	76-77	0.024708229347336186
1106	78-79	0.03176934549822619
1106	80-81	0.03158447510730511
1106	82-83	0.05025444486789965
1106	84-85	0.03358582948576938
1106	86-87	0.06394392887328948
1106	88-89	0.08777032775154936
1106	90-91	0.11073628442535721
1106	92-93	0.08649553728956505
1106	94-95	0.1663201657719604
1106	96-97	0.15818103387825388
1106	98-99	0.14994717473828345
1106	100-101	0.15642143692280897
1107	1	0.1396159770939427
1107	2	0.17251076869087
1107	3	0.2079133595291367
1107	4	0.2510912783914705
1107	5	0.20637196468149455
1107	6	0.19813867315526323
1107	7	0.15201135925500608
1107	8	0.34657726232421737
1107	9	0.24425474402779912
1107	10-11	0.38219759474277737
1107	12-13	0.23316307382881263
1107	14-15	0.3040086851017918
1107	16-17	0.25590309659553867
1107	18-19	0.3716499081320066
1107	20-21	0.3603772923900479
1107	22-23	0.29352718998310223
1107	24-25	0.287637847359008
1107	26-27	0.3346913726499636
1107	28-29	0.3129256526192421
1107	30-31	0.335279533704103
1107	32-33	0.34048658473206217
1107	34-35	0.23605452552217088
1107	36-37	0.24387644118503005
1107	38-39	0.22900442988898817
1107	40-41	0.23942393351781277
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2216	100-101	-1.5920324097705034
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	101500.0
3	19954.0
4	12239.0
5	8329.0
6	7567.0
7	7882.0
8	9600.0
9	10858.0
10	11928.0
11	12920.0
12	14736.0
13	18118.0
14	24858.0
15	38844.0
16	42913.0
17	61963.0
18	94288.0
19	46200.0
20	29573.0
21	26096.0
22	27494.0
23	29934.0
24	34024.0
25	39285.0
26	45807.0
27	55545.0
28	67258.0
29	77147.0
30	89160.0
31	108477.0
32	136932.0
33	177498.0
34	243357.0
35	360000.0
36	610747.0
37	1032922.0
38	1091473.0
39	257486.0
40	228.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.301981736704814	38.94645730630166	16.066663781469956	19.684897175523563
2	22.39980685564092	27.49095914788235	33.168647727789235	16.940586268687493
3	21.569970302006656	30.589110211132176	22.92997903728923	24.910940449571942
4	24.297037936210934	25.84911426160132	28.3251912473576	21.528656554830146
5	22.097115995726234	29.673171106043284	24.235749689601096	23.993963208629385
6	21.76655866008373	24.806195909019564	28.862126980967528	24.565118449929173
7	24.823657593058403	26.777023665066444	23.8112498057903	24.588068936084852
8	27.765390278983254	24.828933525842736	26.135196748817517	21.27047944635649
9	21.35203468556862	28.620255091384738	25.48292291130015	24.5447873117465
10-11	26.35714731752793	25.557649634153595	25.465407466010287	22.61979558230819
12-13	24.772988185296	25.718345449742912	24.963432457580765	24.54523390738032
14-15	24.59850167870556	25.66093284715563	25.203319511051745	24.537245963087067
16-17	24.70227795703826	27.225587638215536	25.085603968833954	22.986530435912258
18-19	23.02769506495724	25.499573088422522	26.84736863740957	24.62536320921067
20-21	22.868613402850634	28.75077544125902	25.589876248032635	22.79073490785771
22-23	21.270523415002632	27.127251491733595	28.84502797593636	22.75719711732742
24-25	21.25516205854516	25.3047571580414	30.686387585726166	22.753693197687273
26-27	21.191439640580313	25.29442498071847	32.61877033066678	20.895365048034435
28-29	21.304619330323472	25.26554290136781	32.58412259472366	20.84571517358506
30-31	21.364337883715727	25.222039485814165	32.467231592584675	20.946391037885437
32-33	21.321202072620384	25.09324136547488	32.67130636251975	20.91425019938498
34-35	21.354668649151765	25.212814760073577	32.562671752684984	20.869844838089673
36-37	21.37050506210833	25.05260971425576	32.47156046259372	21.105324761042187
38-39	21.566393387075184	24.990973245890945	32.39010842825892	21.05252493877495
40-41	22.00799794390856	25.61944623430059	31.043961151045313	21.328594670745538
42-43	22.221795984426844	25.638498479658967	30.65208776026367	21.487617775650524
44-45	22.324746113206835	25.97017371160223	30.287872135167078	21.41720804002386
46-47	22.481181716129917	26.572777129100377	29.442813160941533	21.503227993828173
48-49	22.629221664349913	27.009926511490924	28.21967798523029	22.14117383892887
50-51	22.87555058463947	26.924674522974186	27.64659068587986	22.553184206506483
52-53	23.05165583654866	26.976830150922616	27.325891696983852	22.64562231554487
54-55	23.22526404597899	26.844952445112842	27.111111067388055	22.818672441520114
56-57	23.196733535269114	26.750756078731346	27.340774895116947	22.711735490882592
58-59	23.310309440412567	26.845415155082218	27.2661568448682	22.578118559637012
60-61	23.3179238709666	26.825862910054	27.188563872406778	22.667649346572624
62-63	23.27191014154083	26.971694097413952	27.261992632994637	22.494403128050585
64-65	23.256250916105852	27.10362005684037	27.112650770119163	22.527478256934614
66-67	23.234744378446553	26.852425824177445	27.087742091089417	22.82508770628658
68-69	23.43247817314911	26.771959065538237	27.127819821014263	22.667742940298385
70-71	23.630827453672467	26.948510103444	26.91749067155005	22.503171771333484
72-73	23.601506565776525	26.897352789453056	26.876000430784575	22.625140213985844
74-75	23.491924545820247	26.889959212096116	26.957116146679084	22.66100009540455
76-77	23.47624359644928	27.046298731456186	26.709733043020016	22.767724629074515
78-79	23.46799155407881	27.021341324572006	26.690470286572936	22.820196834776247
80-81	23.280445346156466	27.124238196889827	26.946395204878453	22.64892125207525
82-83	23.152536553910714	27.345251791236308	26.868815150118564	22.633396504734414
84-85	23.13267350653623	27.263067143993762	26.92180489766559	22.682454451804418
86-87	23.0593228417145	27.283271839457946	27.089498993213425	22.56790632561413
88-89	23.137859533759787	27.63217048399273	26.684162758115388	22.54580722413209
90-91	23.128229594471634	27.684007574453435	26.507085065046848	22.680677766028083
92-93	23.166404877318026	27.689591702059623	26.61973245453162	22.524270966090732
94-95	23.193425608020803	27.87687926079794	26.4854509650522	22.44424416612906
96-97	23.216174203706633	27.8489141579265	26.286326864470194	22.64858477389667
98-99	23.265819717243787	27.66196796342274	26.507706615544947	22.564505703788534
100-101	23.490917731628137	27.691624217703776	26.11500324956843	22.702454801099663
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	235.0
1	227.0
2	227.5
3	253.0
4	260.5
5	282.5
6	310.5
7	303.5
8	288.5
9	295.5
10	320.5
11	325.5
12	357.0
13	390.0
14	414.5
15	478.5
16	724.0
17	921.5
18	1136.5
19	1767.5
20	2852.5
21	4437.0
22	6626.0
23	9618.5
24	13681.5
25	18875.0
26	25082.0
27	32774.5
28	41398.0
29	50598.0
30	60840.5
31	72392.0
32	85903.5
33	98650.5
34	108005.5
35	118338.0
36	130924.5
37	145314.0
38	156969.0
39	163275.5
40	168880.0
41	177338.5
42	189962.5
43	200821.0
44	205950.5
45	207165.5
46	207181.5
47	205400.5
48	204317.0
49	199338.0
50	190226.0
51	181618.5
52	164777.5
53	143908.5
54	129188.5
55	118030.0
56	106203.5
57	94836.5
58	87135.5
59	81030.0
60	72439.5
61	63766.0
62	56703.0
63	50047.5
64	43592.0
65	36796.5
66	30837.5
67	25753.0
68	20591.5
69	16166.0
70	12512.5
71	9574.0
72	7251.0
73	5587.0
74	4308.0
75	3073.0
76	2179.5
77	1570.5
78	1122.0
79	695.0
80	401.5
81	257.5
82	151.5
83	111.0
84	78.5
85	53.5
86	38.0
87	27.5
88	26.0
89	26.0
90	25.0
91	23.5
92	19.5
93	12.5
94	9.5
95	7.0
96	4.5
97	6.0
98	6.0
99	4.5
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.033824044175774906
2	0.05763853109255596
3	0.045328152223930905
4	0.06113892636190941
5	0.05840547162909969
6	0.017344655211065967
7	0.008456011043943727
8	0.014021246219376459
9	0.007354763094034776
10-11	0.01371643651895523
12-13	0.014021246219376459
14-15	0.014090074216245767
16-17	0.016233574690175688
18-19	0.018662219722564176
20-21	0.018445903160974917
22-23	0.02042224992822223
24-25	0.01824925174134832
26-27	0.012005569168203825
28-29	0.014650530762181573
30-31	0.007915219639970581
32-33	0.01484718218180817
34-35	0.012044899452129144
36-37	0.024394608604679516
38-39	0.03226066538974345
40-41	0.03359789504320432
42-43	0.037471928009848304
44-45	0.03484663155783321
46-47	0.03381421160479357
48-49	0.04398108999948871
50-51	0.035269432110030405
52-53	0.0381307102655974
54-55	0.05200446792025391
56-57	0.04593777162477336
58-59	0.04815993266655392
60-61	0.047992778959871314
62-63	0.051394848519411465
64-65	0.04886787777720967
66-67	0.03895664622802912
68-69	0.030490802613104065
70-71	0.024188124614071587
72-73	0.027383710183003812
74-75	0.029989341493056237
76-77	0.028258809000342174
78-79	0.03420751444404677
80-81	0.03389287217264421
82-83	0.047343829275103534
84-85	0.04703901957468231
86-87	0.04689153100996236
88-89	0.04878921720935903
90-91	0.04454154654542451
92-93	0.036469005769752656
94-95	0.03261463794507132
96-97	0.03366672304007363
98-99	0.02607597824248693
100-101	0.027295217044171843
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	5085140.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.38921478141369
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.98520453803864	47.62455726913045
2	10.116594582202314	11.611668386689892
3	2.5692871595677853	4.4234811790669175
4	1.1041650904975957	2.5346867013082237
5	0.6074859317200415	1.7431570306084336
6	0.3821104214789465	1.3157410229083062
7	0.29331227838053214	1.1783072939404309
8	0.24657205583347933	1.1320461337057817
9	0.18407813576371637	0.9507689702915532
>10	1.299271737116643	14.004704814938712
>50	0.15492700921907301	6.219686987066426
>100	0.05529184200085901	4.569094368396109
>500	8.669482866302645E-4	0.3540656375167135
>1k	5.895245081005145E-4	0.624385944365752
>5k	2.0806747344597536E-4	0.8700485980649623
>10k+	3.46779122409959E-5	0.8435996620013653
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	42389	0.8335857026551874	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC	8544	0.16801897292896556	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG	8522	0.16758633980578705	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA	8022	0.15775376882445716	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA	6420	0.1262502114002761	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC	6271	0.12332010524783979	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG	5939	0.11679127811623671	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	7.472753945810735E-4	0.0	0.0
2	0.0	0.0	0.0014158902213115077	0.0	0.0
3	0.0	0.0	0.002379482177481839	1.9665141962659827E-5	0.0
4	0.0	0.0	0.0032250832818762117	1.9665141962659827E-5	0.0
5	1.9665141962659827E-5	0.0	0.0043066660898225025	1.9665141962659827E-5	0.0
6	1.9665141962659827E-5	0.0	0.005958538014685928	1.9665141962659827E-5	0.0
7	1.9665141962659827E-5	0.0	0.00688279968693094	3.9330283925319654E-5	1.9665141962659827E-5
8	3.9330283925319654E-5	0.0	0.00792505221095191	3.9330283925319654E-5	1.9665141962659827E-5
9	0.012389039436475692	0.0	0.008731323031420964	3.9330283925319654E-5	1.9665141962659827E-5
10-11	0.03810121255265342	0.0	0.009606421848759327	3.9330283925319654E-5	1.9665141962659827E-5
12-13	0.071885926444503	0.0	0.010668339514742955	3.9330283925319654E-5	1.9665141962659827E-5
14-15	0.08109904545400913	0.0	0.01162209889993196	3.9330283925319654E-5	1.9665141962659827E-5
16-17	0.08395049103859481	3.9330283925319654E-5	0.01253652800119564	3.9330283925319654E-5	1.9665141962659827E-5
18-19	0.08998768962113138	7.866056785063931E-5	0.013696771376992569	3.9330283925319654E-5	1.9665141962659827E-5
20-21	0.10819761107855438	8.849313883196923E-5	0.015515797008538605	3.9330283925319654E-5	1.9665141962659827E-5
22-23	0.13049788206421062	1.376559937386188E-4	0.018406572877049603	3.9330283925319654E-5	1.9665141962659827E-5
24-25	0.14078275131068171	1.376559937386188E-4	0.02638078794290816	3.9330283925319654E-5	1.9665141962659827E-5
26-27	0.14491243112284027	1.376559937386188E-4	0.039998898752050094	3.9330283925319654E-5	1.9665141962659827E-5
28-29	0.15396822899664514	1.376559937386188E-4	0.08457977558139992	3.9330283925319654E-5	1.9665141962659827E-5
30-31	0.16392862340073233	1.376559937386188E-4	0.24819375671072968	3.9330283925319654E-5	1.9665141962659827E-5
32-33	0.1681271312097602	1.376559937386188E-4	0.5574084489315928	3.9330283925319654E-5	1.9665141962659827E-5
34-35	0.17068359966490598	1.376559937386188E-4	0.7766846143862312	3.9330283925319654E-5	1.9665141962659827E-5
36-37	0.1739086829467822	1.376559937386188E-4	0.8698777221472762	3.9330283925319654E-5	1.9665141962659827E-5
38-39	0.18024085865875866	1.376559937386188E-4	0.916513606311724	3.9330283925319654E-5	1.9665141962659827E-5
40-41	0.18815607829872924	1.376559937386188E-4	0.9442119587661304	4.916285490664957E-5	1.9665141962659827E-5
42-43	0.19462591000444432	1.376559937386188E-4	0.9658534474960374	5.8995425887979485E-5	1.9665141962659827E-5
44-45	0.20173485882394585	1.376559937386188E-4	0.9761973121683966	5.8995425887979485E-5	1.9665141962659827E-5
46-47	0.2102596978647589	1.376559937386188E-4	0.9789995948980755	6.88279968693094E-5	1.9665141962659827E-5
48-49	0.22033808312062203	1.376559937386188E-4	0.9804843131162564	7.866056785063931E-5	1.9665141962659827E-5
50-51	0.23223549400803123	1.376559937386188E-4	0.9813299142206507	7.866056785063931E-5	1.9665141962659827E-5
52-53	0.2441525700374031	1.376559937386188E-4	0.981723217059904	7.866056785063931E-5	1.9665141962659827E-5
54-55	0.25786900655635836	1.5732113570127862E-4	0.9820968547571944	7.866056785063931E-5	1.9665141962659827E-5
56-57	0.2737289435492435	1.5732113570127862E-4	0.9823623341736905	7.866056785063931E-5	1.9665141962659827E-5
58-59	0.29257798212045294	1.5732113570127862E-4	0.9825196553093917	7.866056785063931E-5	1.9665141962659827E-5
60-61	0.31388516343699485	1.5732113570127862E-4	0.9826278135901862	7.866056785063931E-5	1.9665141962659827E-5
62-63	0.3374243383662987	1.5732113570127862E-4	0.9826966415870556	7.866056785063931E-5	1.9665141962659827E-5
64-65	0.36332333033112163	1.5732113570127862E-4	0.9827163067290183	8.849313883196923E-5	1.9665141962659827E-5
66-67	0.3932340112563273	1.5732113570127862E-4	0.9827359718709809	1.1799085177595897E-4	1.9665141962659827E-5
68-69	0.4261731240437825	1.5732113570127862E-4	0.982765469583925	1.1799085177595897E-4	1.9665141962659827E-5
70-71	0.464933118852185	1.5732113570127862E-4	0.982765469583925	1.376559937386188E-4	1.9665141962659827E-5
72-73	0.5062790798286773	1.5732113570127862E-4	0.982765469583925	1.376559937386188E-4	1.9665141962659827E-5
74-75	0.5513909154910189	1.5732113570127862E-4	0.982765469583925	1.376559937386188E-4	1.9665141962659827E-5
76-77	0.6017533440573908	1.6715370668260855E-4	0.982765469583925	1.376559937386188E-4	1.9665141962659827E-5
78-79	0.6569435649755955	1.7698627766393846E-4	0.982765469583925	1.376559937386188E-4	1.9665141962659827E-5
80-81	0.7185052918897021	1.7698627766393846E-4	0.982765469583925	1.376559937386188E-4	1.9665141962659827E-5
82-83	0.7862418733800839	1.7698627766393846E-4	0.982765469583925	1.5732113570127862E-4	1.9665141962659827E-5
84-85	0.8592487129164585	1.7698627766393846E-4	0.982765469583925	1.6715370668260855E-4	1.9665141962659827E-5
86-87	0.9402985955155609	1.7698627766393846E-4	0.982765469583925	1.7698627766393846E-4	3.9330283925319654E-5
88-89	1.0289490554832317	1.7698627766393846E-4	0.982765469583925	1.7698627766393846E-4	3.9330283925319654E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACG	16880	0.0	90.86252	1
TCGACGG	17650	0.0	86.63104	2
TATCTCG	19415	0.0	86.07305	2
CGACGGC	18700	0.0	81.71592	3
GACGGCG	18810	0.0	80.88439	4
ATCTCGA	20750	0.0	80.46664	3
ACGGCGC	18865	0.0	80.3739	5
ATATCTC	21075	0.0	79.76528	1
TCTCGAG	20175	0.0	79.462776	4
CGGCGCG	19480	0.0	77.80121	6
CTCGAGG	20875	0.0	75.935455	5
TCGAGGG	21080	0.0	75.11791	6
AGGGCGC	21645	0.0	73.06216	9
GAGGGCG	23255	0.0	68.02497	8
CGAGGGC	23350	0.0	67.78889	7
CTCGACG	1135	0.0	53.168316	5
TCTCGAC	1360	0.0	46.11766	4
CGCGCCG	40820	0.0	39.160378	9
GGCGCGC	40875	0.0	38.840843	7
GCGCGCC	41200	0.0	38.69583	8
>>END_MODULE