FastQCFastQC Report
Thu 26 May 2016
SRR2048268_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048268_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5085140
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT300060.5900722497315708No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT247910.48751853439629983No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129390.2544472718548555No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT57910.11388083710576306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG19350.080.2556841
TCGACGG25850.062.8508952
TATCTCG77500.058.2328152
ATATCTC86000.052.732421
ATCTCGA153100.048.4454041
TCTCGAG160050.045.3537862
CGACGGC38200.042.647273
GACGGCG41150.039.7053534
ACGGCGC42450.038.4894075
CTCGAGG204200.035.5641333
TCGAGGG207200.035.003364
CGGCGCG47750.034.3167536
TATATGT86450.031.7558174
CGAGGGC242650.029.9678335
AGGGCGC252600.028.8249977
GAGGGCG257850.028.2012626
ACATATA98650.028.200381
GGGCGCG258050.028.1796828
ATATATG103600.026.9115563
CATATAT108250.025.7606072
Produced by FastQC (version 0.11.5)