FastQCFastQC Report
Thu 26 May 2016
SRR2048267_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048267_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7253597
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT540480.7451199728906913No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG113370.15629486997968042No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC113200.1560605034991605No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA100550.1386208800957649No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC82580.11384696447845119No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG78650.10842896289937254No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA77480.10681597006285296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG233650.089.810091
TCGACGG247900.084.6092152
TATCTCG257700.083.4568562
CGACGGC262550.079.978613
GACGGCG267350.078.4015664
ACGGCGC266850.078.1232455
ATCTCGA275400.077.972293
ATATCTC278800.077.5838551
TCTCGAG264250.076.569984
CGGCGCG273150.076.29726
CTCGAGG272300.073.7673955
TCGAGGG274200.073.129736
AGGGCGC283700.070.690349
CGAGGGC304600.065.8711247
GAGGGCG309000.064.8870548
CTCGACG18700.060.7345925
TCTCGAC22250.051.897234
CATATGT192300.044.232514
ACATATG202650.042.8404273
CGCGCCG554500.039.7387969
Produced by FastQC (version 0.11.5)