##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2048267_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7253597
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.12833150228776	31.0	31.0	34.0	28.0	34.0
2	31.33755707685442	33.0	31.0	34.0	28.0	34.0
3	31.404285349737517	33.0	31.0	34.0	28.0	34.0
4	34.907394910414794	37.0	35.0	37.0	32.0	37.0
5	34.83503756825751	37.0	35.0	37.0	32.0	37.0
6	34.8245529769575	37.0	35.0	37.0	32.0	37.0
7	34.74646316854934	37.0	35.0	37.0	32.0	37.0
8	34.54342569624422	37.0	35.0	37.0	31.0	37.0
9	36.170868604914226	39.0	37.0	39.0	32.0	39.0
10-11	35.912678564855476	39.0	37.0	39.0	30.5	39.0
12-13	35.9848954939184	39.0	37.0	39.0	30.5	39.0
14-15	37.256831610578864	40.0	38.0	41.0	31.0	41.0
16-17	37.23837642207032	40.0	38.0	41.0	31.0	41.0
18-19	37.23149714548519	40.0	38.0	41.0	31.0	41.0
20-21	37.25821788831114	40.0	38.0	41.0	31.0	41.0
22-23	37.30390466964184	40.0	38.0	41.0	31.0	41.0
24-25	37.36216941746281	40.0	38.0	41.0	31.5	41.0
26-27	37.298424629876735	40.0	38.0	41.0	31.0	41.0
28-29	37.18962419610574	40.0	37.5	41.0	31.0	41.0
30-31	36.999855313163934	40.0	37.0	41.0	31.0	41.0
32-33	36.76363051324743	40.0	36.0	41.0	31.0	41.0
34-35	36.65071391476532	40.0	35.5	41.0	30.0	41.0
36-37	36.549496959922095	40.0	35.0	41.0	30.0	41.0
38-39	36.42941550240522	40.0	35.0	41.0	30.0	41.0
40-41	36.02344767706284	40.0	35.0	41.0	28.5	41.0
42-43	35.834266364673965	40.0	35.0	41.0	28.0	41.0
44-45	35.62539544173739	40.0	35.0	41.0	27.0	41.0
46-47	35.31498820516221	39.0	35.0	41.0	25.5	41.0
48-49	34.85236917628592	39.0	35.0	41.0	23.0	41.0
50-51	34.53604087737436	39.0	34.5	41.0	20.5	41.0
52-53	34.32738391724823	39.0	34.0	41.0	18.5	41.0
54-55	34.119604177072425	39.0	34.0	41.0	18.0	41.0
56-57	33.89332657990236	38.5	34.0	40.0	16.0	41.0
58-59	33.668458559249984	38.0	33.5	40.0	13.5	41.0
60-61	33.41176012397711	38.0	33.0	40.0	11.0	41.0
62-63	33.11544134585917	37.0	33.0	40.0	8.0	41.0
64-65	32.813211086858004	37.0	33.0	40.0	7.0	41.0
66-67	32.497639033434034	36.0	32.5	39.5	4.5	41.0
68-69	32.14333012986522	35.5	32.0	39.0	2.0	41.0
70-71	31.818041035364935	35.0	32.0	39.0	2.0	40.5
72-73	31.47632953691803	35.0	32.0	38.0	2.0	40.0
74-75	31.12321597133119	35.0	31.0	37.0	2.0	39.5
76-77	30.793845522435284	35.0	31.0	37.0	2.0	39.0
78-79	30.46637557890244	35.0	31.0	36.0	2.0	39.0
80-81	30.14440869819484	35.0	31.0	36.0	2.0	38.0
82-83	29.88971733610235	35.0	31.0	35.5	2.0	37.0
84-85	29.636065802938873	35.0	31.0	35.0	2.0	37.0
86-87	29.359320623960773	34.5	30.0	35.0	2.0	36.0
88-89	29.128106441535145	34.0	30.0	35.0	2.0	36.0
90-91	28.924898571012424	34.0	30.0	35.0	2.0	36.0
92-93	28.724924337539015	34.0	30.0	35.0	2.0	35.5
94-95	28.527194852429766	34.0	30.0	35.0	2.0	35.0
96-97	28.32383457476339	34.0	29.5	35.0	2.0	35.0
98-99	28.054696724949014	34.0	29.0	35.0	2.0	35.0
100-101	26.822268730948245	33.0	26.5	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.7078277348659405
1101	2	0.7389529247316986
1101	3	0.7526836744397656
1101	4	0.7600682246535371
1101	5	0.7785332627798098
1101	6	0.7801700199248174
1101	7	0.8046710108506474
1101	8	0.904513433902693
1101	9	0.959332248754798
1101	10-11	1.0794606002709841
1101	12-13	1.0106490804799506
1101	14-15	1.1232001745063585
1101	16-17	1.067528269310209
1101	18-19	1.1097493667104743
1101	20-21	1.0936268303004653
1101	22-23	1.0869753285064547
1101	24-25	1.0744467231172976
1101	26-27	1.114109938259034
1101	28-29	1.146880308762121
1101	30-31	1.1002562431724172
1101	32-33	1.1343332984486096
1101	34-35	1.0908159697033142
1101	36-37	1.054878797535487
1101	38-39	1.1395091424518995
1101	40-41	1.1838107398109088
1101	42-43	1.02612905068613
1101	44-45	0.9904809240050199
1101	46-47	0.9414103931580513
1101	48-49	0.8451766388304591
1101	50-51	0.7121594220089875
1101	52-53	0.5890735258702691
1101	54-55	0.5591729240337173
1101	56-57	0.5694069300371254
1101	58-59	0.632164595277743
1101	60-61	0.6343976607624029
1101	62-63	0.6209205642781939
1101	64-65	0.6268677649961134
1101	66-67	0.5872990961395672
1101	68-69	0.5774825009564921
1101	70-71	0.5676166658292985
1101	72-73	0.5383472643600342
1101	74-75	0.6250748325034508
1101	76-77	0.6217347726518057
1101	78-79	0.5264870880754735
1101	80-81	0.5697465376655515
1101	82-83	0.5887748433165569
1101	84-85	0.5812492522886643
1101	86-87	0.548529563615304
1101	88-89	0.6334604429885751
1101	90-91	0.6522606961115294
1101	92-93	0.6841921348210782
1101	94-95	0.7415730826672551
1101	96-97	0.760173193516934
1101	98-99	0.7261837338811112
1101	100-101	0.787168665056118
1102	1	0.6694487038571424
1102	2	0.6930354700093773
1102	3	0.6338611041543132
1102	4	0.7143608282754599
1102	5	0.6673090297562396
1102	6	0.7270282906003658
1102	7	0.7630816242397813
1102	8	0.8983668813210457
1102	9	0.8284425123498664
1102	10-11	1.002803525065616
1102	12-13	0.9406263118878684
1102	14-15	1.0405811959442914
1102	16-17	1.0153141893953546
1102	18-19	1.0606087074564385
1102	20-21	1.0054601459003578
1102	22-23	0.9793616447139968
1102	24-25	1.0018078980118759
1102	26-27	1.0374864290777595
1102	28-29	1.091114149464559
1102	30-31	1.049810190414135
1102	32-33	1.063005136352288
1102	34-35	1.058370906456986
1102	36-37	1.0676148298466472
1102	38-39	1.05954310693717
1102	40-41	1.0974607352560355
1102	42-43	0.9497716154075633
1102	44-45	0.8735288113883328
1102	46-47	0.7784811288256819
1102	48-49	0.6091656944297981
1102	50-51	0.5149470478256362
1102	52-53	0.4399606867144712
1102	54-55	0.4338547867011826
1102	56-57	0.49290007181264883
1102	58-59	0.484191340685733
1102	60-61	0.5148391644221135
1102	62-63	0.4682233790455683
1102	64-65	0.4569985627688311
1102	66-67	0.4392132329506353
1102	68-69	0.4624266160528734
1102	70-71	0.44502986445194637
1102	72-73	0.4424485560816933
1102	74-75	0.42498271000897603
1102	76-77	0.41018811811607137
1102	78-79	0.4067510166400474
1102	80-81	0.4163498076170882
1102	82-83	0.4249571982135265
1102	84-85	0.38071781189621845
1102	86-87	0.3768350193430159
1102	88-89	0.3345490071957613
1102	90-91	0.4206117467713959
1102	92-93	0.46751348785090485
1102	94-95	0.48802854661684236
1102	96-97	0.5124331975275318
1102	98-99	0.535139495593814
1102	100-101	0.5114808755672051
1103	1	0.5421638783917402
1103	2	0.5542360267109423
1103	3	0.5405591378483834
1103	4	0.5870892008210475
1103	5	0.5733973363752938
1103	6	0.543233778099875
1103	7	0.5557005940028361
1103	8	0.700653630751475
1103	9	0.7303244498633106
1103	10-11	0.8376203796849353
1103	12-13	0.7677755795833505
1103	14-15	0.8526371078508177
1103	16-17	0.8255173003695049
1103	18-19	0.882551817277573
1103	20-21	0.8441831017298398
1103	22-23	0.8252119891968093
1103	24-25	0.8495803829373756
1103	26-27	0.8469083808046634
1103	28-29	0.8411723942325722
1103	30-31	0.842514242653607
1103	32-33	0.9027652928422043
1103	34-35	0.8463545770154539
1103	36-37	0.8819004904990138
1103	38-39	0.8858818905617838
1103	40-41	0.8381027407748931
1103	42-43	0.8136773910317388
1103	44-45	0.7014626614008392
1103	46-47	0.7250597027476786
1103	48-49	0.5589066853044926
1103	50-51	0.42025394280521056
1103	52-53	0.39467109154048075
1103	54-55	0.39913089866094964
1103	56-57	0.36467346572881354
1103	58-59	0.31969039050255077
1103	60-61	0.35554926221296057
1103	62-63	0.3899067551653559
1103	64-65	0.39585327681336224
1103	66-67	0.35772001082861493
1103	68-69	0.3811849711562658
1103	70-71	0.3393880996521972
1103	72-73	0.39622996816286715
1103	74-75	0.3740096359074343
1103	76-77	0.30965084444066093
1103	78-79	0.3559763701642815
1103	80-81	0.3807580635944774
1103	82-83	0.3015990859913913
1103	84-85	0.2956282632339118
1103	86-87	0.3371016395667077
1103	88-89	0.3672756418104335
1103	90-91	0.33276011731761557
1103	92-93	0.3963483613412926
1103	94-95	0.36125160795680245
1103	96-97	0.3768398928795591
1103	98-99	0.36590459238844986
1103	100-101	0.41257835170157264
1104	1	0.4479060421036216
1104	2	0.5425828868683773
1104	3	0.5355372162522514
1104	4	0.5217376111509893
1104	5	0.501413411328059
1104	6	0.5053432169748788
1104	7	0.5193511147323804
1104	8	0.7230307369443878
1104	9	0.6886682504789334
1104	10-11	0.8177963012444778
1104	12-13	0.7077657600041505
1104	14-15	0.8509790414616916
1104	16-17	0.7694384893094153
1104	18-19	0.8485612992040075
1104	20-21	0.8041440189109323
1104	22-23	0.7566520698648134
1104	24-25	0.7231793788044172
1104	26-27	0.7856613389319591
1104	28-29	0.8612320127072906
1104	30-31	0.8175859240456873
1104	32-33	0.8414310798615077
1104	34-35	0.8136081934818549
1104	36-37	0.8215230301756549
1104	38-39	0.818831913064983
1104	40-41	0.8151191024113373
1104	42-43	0.754082550611777
1104	44-45	0.7087994175296615
1104	46-47	0.6992687846575265
1104	48-49	0.6366664206450565
1104	50-51	0.5836744951900457
1104	52-53	0.5405750339367472
1104	54-55	0.496207360534747
1104	56-57	0.523859429316218
1104	58-59	0.5090199277325453
1104	60-61	0.5188107631088243
1104	62-63	0.5162599321385528
1104	64-65	0.4962055849381315
1104	66-67	0.49004781245636053
1104	68-69	0.48937782646284234
1104	70-71	0.4576009543918289
1104	72-73	0.4704002926181303
1104	74-75	0.5058742821128277
1104	76-77	0.5037201928448347
1104	78-79	0.5578001768626812
1104	80-81	0.5439853174511278
1104	82-83	0.5280471771850088
1104	84-85	0.5543255449615785
1104	86-87	0.5479127630609035
1104	88-89	0.5912703044390462
1104	90-91	0.49820599282205436
1104	92-93	0.48868696701969583
1104	94-95	0.5593035972664175
1104	96-97	0.555926423452707
1104	98-99	0.5764787445246178
1104	100-101	0.4672732472379799
1105	1	0.3573160907986903
1105	2	0.38595407634358736
1105	3	0.38566389049031713
1105	4	0.40316875501005
1105	5	0.3921223241357481
1105	6	0.384662558758059
1105	7	0.3705754168983191
1105	8	0.5796641102799143
1105	9	0.49938776890278547
1105	10-11	0.6668170230591244
1105	12-13	0.6229482009112246
1105	14-15	0.6378997383208755
1105	16-17	0.6469346526614146
1105	18-19	0.6553305549795851
1105	20-21	0.6172147896810998
1105	22-23	0.6372732080333208
1105	24-25	0.6068130446553113
1105	26-27	0.6663815927020664
1105	28-29	0.6846958022764227
1105	30-31	0.6672328671917285
1105	32-33	0.7065766628068104
1105	34-35	0.6865544979511355
1105	36-37	0.6495615097339709
1105	38-39	0.6885752468454882
1105	40-41	0.7363536414287282
1105	42-43	0.5668685894626364
1105	44-45	0.5426937715558822
1105	46-47	0.5325851557633072
1105	48-49	0.5250425857861103
1105	50-51	0.43541638259656423
1105	52-53	0.3626501941840061
1105	54-55	0.3615286216599358
1105	56-57	0.32504086982204683
1105	58-59	0.34189900179121224
1105	60-61	0.28958070462540064
1105	62-63	0.37047957669702214
1105	64-65	0.3101302382297746
1105	66-67	0.29095703151625685
1105	68-69	0.3782772720523866
1105	70-71	0.3637782939208236
1105	72-73	0.39215107375623504
1105	74-75	0.40686027827051774
1105	76-77	0.41730591102512093
1105	78-79	0.38207421416870346
1105	80-81	0.3898651273591369
1105	82-83	0.34203681279886666
1105	84-85	0.32105701929742025
1105	86-87	0.32442609868921224
1105	88-89	0.36732308758795895
1105	90-91	0.40151101594132044
1105	92-93	0.37276890268493545
1105	94-95	0.40291447905839917
1105	96-97	0.4058683356542616
1105	98-99	0.4251610950534861
1105	100-101	0.4151204866506326
1106	1	0.41354657650711246
1106	2	0.44425429212162726
1106	3	0.4374583336401905
1106	4	0.46819007509127886
1106	5	0.4724950280179172
1106	6	0.5016595562379393
1106	7	0.5290618384856174
1106	8	0.6542837562517505
1106	9	0.5381036127499357
1106	10-11	0.7164462026840042
1106	12-13	0.6682185687385243
1106	14-15	0.7242713166620334
1106	16-17	0.732906586713149
1106	18-19	0.7379200804838391
1106	20-21	0.7396356365034507
1106	22-23	0.6834016476417517
1106	24-25	0.6730656611868611
1106	26-27	0.7083467329457491
1106	28-29	0.737525812147382
1106	30-31	0.7508050115284277
1106	32-33	0.7766414865773044
1106	34-35	0.7419251916152945
1106	36-37	0.7412406114132892
1106	38-39	0.7501835286413225
1106	40-41	0.8043738011743571
1106	42-43	0.7149306555426733
1106	44-45	0.6487593659680471
1106	46-47	0.6810881207590569
1106	48-49	0.7144675325115983
1106	50-51	0.6810059334792342
1106	52-53	0.6820995327016277
1106	54-55	0.6769679679476397
1106	56-57	0.7015657325724405
1106	58-59	0.6750897886883749
1106	60-61	0.6805773036742266
1106	62-63	0.6944355926831136
1106	64-65	0.7043590668539821
1106	66-67	0.6970670793387583
1106	68-69	0.7137412508785133
1106	70-71	0.710933199152926
1106	72-73	0.7491863193856787
1106	74-75	0.7434579693568715
1106	76-77	0.6875812787066948
1106	78-79	0.6982706392101967
1106	80-81	0.7120321149499453
1106	82-83	0.7021742406373406
1106	84-85	0.7068152178972511
1106	86-87	0.7015370415103028
1106	88-89	0.7159137878146389
1106	90-91	0.746892108052915
1106	92-93	0.741111084825512
1106	94-95	0.7562384833141884
1106	96-97	0.7251667889864954
1106	98-99	0.77197568696646
1106	100-101	0.7404596892682882
1107	1	0.2622262191244644
1107	2	0.2839722730195078
1107	3	0.3345716172330313
1107	4	0.3508397830482366
1107	5	0.3019303194095855
1107	6	0.28846127581048364
1107	7	0.3080923611811883
1107	8	0.4673401851075738
1107	9	0.34781722170524887
1107	10-11	0.5181703973549716
1107	12-13	0.36634745692953175
1107	14-15	0.41819911057897485
1107	16-17	0.3779563874372869
1107	18-19	0.46023952293947445
1107	20-21	0.45587958981636234
1107	22-23	0.38074381051133344
1107	24-25	0.36533982221017425
1107	26-27	0.41547886013132995
1107	28-29	0.4238751765536648
1107	30-31	0.442997842408559
1107	32-33	0.39795865482670223
1107	34-35	0.354394444247049
1107	36-37	0.39922841253776653
1107	38-39	0.4005192430963831
1107	40-41	0.40385114610735684
1107	42-43	0.238090694828351
1107	44-45	0.24274368333063734
1107	46-47	0.21361913052765402
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2209	7	0.29126113567092204
2209	8	0.3681178187698677
2209	9	0.31400839340575715
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2210	26-27	-0.13750826202948474
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2210	30-31	-0.08532073788789063
2210	32-33	-0.040055672647831386
2210	34-35	-0.05551600140704238
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2211	14-15	-0.146047097929781
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2211	32-33	-0.11305258899483306
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2214	3	-0.9465369847369445
2214	4	-0.9122964865018517
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2214	9	-1.6197083333154083
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2214	24-25	-1.2251653387207426
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2214	30-31	-1.0984598740588183
2214	32-33	-1.0043621032041017
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2214	92-93	-0.793225580411935
2214	94-95	-0.8006187633295845
2214	96-97	-0.8092612373111372
2214	98-99	-0.7737162277718816
2214	100-101	-0.7162024264271913
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2215	3	-0.7804951630350381
2215	4	-0.7893737981044495
2215	5	-0.8078929147065992
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2215	12-13	-1.1103747744965062
2215	14-15	-0.9395335421978146
2215	16-17	-0.9406574125032918
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2215	24-25	-1.0911027841004852
2215	26-27	-1.233134519063313
2215	28-29	-1.1689650425497717
2215	30-31	-1.1502483307757245
2215	32-33	-1.0898709404420117
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2215	62-63	-0.7323282020017388
2215	64-65	-0.6995170949695222
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2215	80-81	-0.5472197018959903
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2215	84-85	-0.7174944583500817
2215	86-87	-0.7144230620889509
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2215	90-91	-0.8633822414450343
2215	92-93	-0.7799801675378184
2215	94-95	-0.769940844696503
2215	96-97	-0.838469586466676
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2216	1	-0.9932022908393812
2216	2	-1.045585714445366
2216	3	-1.1698083477243166
2216	4	-1.0240058849761553
2216	5	-1.0278980562101552
2216	6	-1.0783142141455073
2216	7	-1.091513972456994
2216	8	-1.0684664489908542
2216	9	-1.2838585646213616
2216	10-11	-1.155010778621957
2216	12-13	-1.1775275493909305
2216	14-15	-1.5403244544126764
2216	16-17	-1.3663468325796018
2216	18-19	-1.2940588394864534
2216	20-21	-1.3140655148980116
2216	22-23	-1.2901803562154868
2216	24-25	-1.3722363960477324
2216	26-27	-1.6733386249584967
2216	28-29	-1.533438145503581
2216	30-31	-1.4398921040455122
2216	32-33	-1.4623466029995527
2216	34-35	-1.4214909377877518
2216	36-37	-1.480345524759656
2216	38-39	-1.6817980300984061
2216	40-41	-1.5133452852795273
2216	42-43	-1.4864543866734294
2216	44-45	-1.3321779904592077
2216	46-47	-1.240317679730687
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2216	50-51	-1.3156780209665868
2216	52-53	-1.2318619136491407
2216	54-55	-1.225871310040354
2216	56-57	-1.2600851124896835
2216	58-59	-1.1628112492639318
2216	60-61	-1.1865015618612702
2216	62-63	-1.105363338740787
2216	64-65	-1.015666777913296
2216	66-67	-0.9876499060173707
2216	68-69	-1.103926132660746
2216	70-71	-1.0994012917031242
2216	72-73	-1.0586150390164093
2216	74-75	-1.0663626580487033
2216	76-77	-1.0672302994301326
2216	78-79	-1.1263139446131696
2216	80-81	-1.0723690105314319
2216	82-83	-1.146123973084034
2216	84-85	-1.169054891497744
2216	86-87	-1.165581129689226
2216	88-89	-1.178459392229314
2216	90-91	-1.310858713017513
2216	92-93	-1.239598417377735
2216	94-95	-1.1810376152708457
2216	96-97	-1.2878468549688549
2216	98-99	-1.321019086407869
2216	100-101	-1.444869106439132
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	121480.0
3	26353.0
4	15453.0
5	10893.0
6	9780.0
7	10600.0
8	13035.0
9	14775.0
10	16176.0
11	17655.0
12	20132.0
13	25545.0
14	34719.0
15	54136.0
16	58779.0
17	83495.0
18	125980.0
19	61564.0
20	39443.0
21	35832.0
22	37704.0
23	41855.0
24	48308.0
25	56198.0
26	65994.0
27	79947.0
28	96923.0
29	111842.0
30	127390.0
31	154211.0
32	193744.0
33	245393.0
34	331606.0
35	497691.0
36	868114.0
37	1499910.0
38	1620908.0
39	379715.0
40	319.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.365591363743118	39.14819856638842	15.854761634527376	19.63144843534109
2	22.240830425198126	27.305544623753587	34.149818242411776	16.303806708636507
3	21.4758871882266	31.44099521431848	22.615510529244133	24.46760706821079
4	24.244410675089142	25.519369373843315	27.601909513577823	22.634310437489724
5	21.752579253630376	30.579825816924583	24.024165363567445	23.643429565877597
6	21.718955448777347	24.70036192077233	28.88648417299835	24.69419845745197
7	24.60592575598226	27.56537066031633	23.713654089344086	24.11504949435733
8	27.054818461029356	24.6562221004264	27.23618852614416	21.052770912400078
9	21.963745769542413	28.447784155012158	25.26256170329283	24.325908372152597
10-11	25.9941446930645	25.689066462821575	25.779820549030507	22.536968295083422
12-13	24.594799834403243	25.925902690188945	25.14548143878901	24.333816036618806
14-15	24.45960183212321	25.801064422827274	25.285557434889622	24.45377631015989
16-17	24.616376749965667	27.261525381568763	25.175251653653092	22.946846214812474
18-19	23.04097781300355	25.638145374149268	26.885976052174588	24.434900760672594
20-21	22.880484028615204	28.58668186368777	25.71605402066669	22.816780087030338
22-23	21.435291590206727	27.1132244113619	28.73681565943627	22.714668338995093
24-25	21.439961479575356	25.38526457248168	30.4727501019855	22.702023845957463
26-27	21.396770564655508	25.236866208732913	32.24910147540448	21.1172617512071
28-29	21.48872066620375	25.317625951457668	32.27632437446881	20.917329007869778
30-31	21.527062893719535	25.299242529475475	32.0717372752859	21.101957301519096
32-33	21.52060119680655	25.207119177515736	32.21970537749431	21.05257424818341
34-35	21.580696118974778	25.310005367658405	32.14644152375317	20.962856989613655
36-37	21.51770698832855	25.337092515046873	32.05787895082784	21.08732154579674
38-39	21.63743915911164	25.26737513443486	31.982743242711177	21.112442463742322
40-41	22.040467213591228	25.749857209951056	30.805069706988547	21.404605869469172
42-43	22.273547621530103	25.727713205557016	30.400958736116696	21.597780436796178
44-45	22.429088345417483	26.03723176479139	29.944859707522824	21.588820182268304
46-47	22.578944410598854	26.539064765291453	29.201886480852647	21.680104343257046
48-49	22.768614436286803	26.814238861226535	28.16215047955768	22.25499622292898
50-51	23.13548467709915	26.83142967907753	27.553507650589676	22.479577993233647
52-53	23.27372850534824	26.94494696304453	27.245628318042165	22.535696213565064
54-55	23.417514004593805	26.699662223487554	27.098999360530907	22.783824411387737
56-57	23.25064231141768	26.58120674893356	27.392124267216722	22.77602667243204
58-59	23.32766256549785	26.75572999733657	27.197423685667143	22.71918375149844
60-61	23.405299922165852	26.684037626873597	27.026649955013987	22.884012495946568
62-63	23.45968795471201	26.721689937944333	27.17481397024269	22.64380813710097
64-65	23.46532032769185	26.91352888876138	27.025101579562545	22.59604920398423
66-67	23.46113972058173	26.83723118311083	26.907410546309485	22.794218549997954
68-69	23.365006415038476	26.867920125720552	27.052707158647866	22.714366300593113
70-71	23.44591169540065	27.149395019835055	26.81145860453007	22.593234680234225
72-73	23.359739687810986	27.190942192692496	26.750460122561908	22.698857996934606
74-75	23.289817167564212	27.232694692786524	26.886311704145694	22.59117643550357
76-77	23.33573445750165	27.40138905734145	26.74587319568344	22.517003289473458
78-79	23.35315029900596	27.46005875582297	26.600890687745434	22.58590025742564
80-81	23.293992017822024	27.43081205652412	26.71277598757197	22.562419938081888
82-83	23.303819123326722	27.61108966303346	26.563037279517555	22.522053934122262
84-85	23.34192770821773	27.408697352902188	26.581238773416775	22.668136165463302
86-87	23.299103545625048	27.297659155340643	26.765067823723697	22.63816947531061
88-89	23.381380055081706	27.335134539418647	26.5699920702978	22.71349333520185
90-91	23.439047353179546	27.19672084569377	26.504582441184372	22.859649359942313
92-93	23.474821098869274	27.12668320492907	26.70904323451176	22.689452461689903
94-95	23.552817865804563	27.272840107867385	26.55809594261636	22.616246083711687
96-97	23.529244649214462	27.222579756081227	26.38670214009356	22.86147345461075
98-99	23.557446937608983	27.14771419568496	26.554045621624457	22.740793245081605
100-101	23.73087823443373	27.262475504275272	26.148398808222357	22.858247453068635
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	266.0
1	272.5
2	258.5
3	264.0
4	292.0
5	289.5
6	299.5
7	320.0
8	319.0
9	304.0
10	323.0
11	339.0
12	353.0
13	395.0
14	429.0
15	514.5
16	801.0
17	1004.5
18	1241.5
19	1881.0
20	2996.5
21	4854.0
22	7531.5
23	11113.0
24	16209.0
25	23047.0
26	31356.0
27	41407.5
28	52820.5
29	66083.0
30	80681.0
31	97526.5
32	117993.5
33	137451.5
34	151887.5
35	168709.0
36	189472.0
37	212705.0
38	231947.5
39	242343.0
40	250625.0
41	261861.5
42	279023.0
43	293143.0
44	298977.0
45	297979.5
46	295280.5
47	292560.5
48	289956.5
49	282970.5
50	270745.5
51	257886.5
52	234576.0
53	205817.0
54	185886.5
55	170417.5
56	153775.5
57	137249.5
58	125060.5
59	115375.0
60	102113.0
61	89062.0
62	79009.5
63	70165.0
64	61170.5
65	51955.5
66	43572.5
67	35981.0
68	29005.0
69	23090.5
70	18161.5
71	13842.0
72	10513.5
73	8127.0
74	6212.5
75	4543.0
76	3154.5
77	2243.0
78	1561.5
79	1027.0
80	621.5
81	355.0
82	203.0
83	127.5
84	93.0
85	77.0
86	61.0
87	40.5
88	27.0
89	19.0
90	22.5
91	21.5
92	12.0
93	9.5
94	8.0
95	6.0
96	7.5
97	7.5
98	9.5
99	8.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03308703254399162
2	0.05660639817734567
3	0.044377982399628764
4	0.06094907119874456
5	0.05808152837826529
6	0.016281577264355876
7	0.008947285050437735
8	0.014089561358316433
9	0.007444582322398115
10-11	0.0142136377303564
12-13	0.014330820970616371
14-15	0.014172278939676413
16-17	0.01611614210163592
18-19	0.018349516798355354
20-21	0.017901463232655467
22-23	0.019755715681474998
24-25	0.01848048630217532
26-27	0.01204919435143695
28-29	0.014351500365956366
30-31	0.007933994678777992
32-33	0.015578477822796054
34-35	0.012407637203996858
36-37	0.025387404345733573
38-39	0.03320421578425159
40-41	0.035382445426731045
42-43	0.03861532423155022
44-45	0.03636816327127079
46-47	0.03487235367501117
48-49	0.045081081841188586
50-51	0.03747106435607051
52-53	0.039952591796869885
54-55	0.052842748225466626
56-57	0.04707319692560808
58-59	0.04894123563798761
60-61	0.04916181585494755
62-63	0.05260838174494668
64-65	0.04983734276938738
66-67	0.03984230168838991
68-69	0.03138442899433205
70-71	0.024663625508833754
72-73	0.02752427519753303
74-75	0.030750260870572213
76-77	0.029399207041692556
78-79	0.03544448361275102
80-81	0.035410017953851035
82-83	0.04866551036678768
84-85	0.048334640041347764
86-87	0.04796241092522786
88-89	0.0501819993583873
90-91	0.04567389117426844
92-93	0.03769853770481045
94-95	0.034065857256751375
96-97	0.03495507125637115
98-99	0.027462237011513048
100-101	0.02862717628233275
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	7253597.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.621676887562465
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.39238073094464	44.457883213951234
2	10.980637306347095	11.995616459336105
3	3.0993342436353077	5.078725008672167
4	1.274699361610841	2.7850486663475804
5	0.646239114062511	1.7649332040213552
6	0.4047525145309353	1.3264956640882306
7	0.2752832803725841	1.052550407514169
8	0.21356961992796294	0.9332424618163773
9	0.17446089236027798	0.8576411842817027
>10	1.303357848499332	13.816319850098566
>50	0.14461809763641298	5.650256923194872
>100	0.0887494450224307	7.508206467115806
>500	0.0010227204733675478	0.392206870707188
>1k	6.902932463575908E-4	0.7570600276301664
>5k	1.0226566490926043E-4	0.4122270865707
>10k+	1.0226566490926043E-4	1.2115865046537464
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	54048	0.7451199728906913	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG	11337	0.15629486997968042	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC	11320	0.1560605034991605	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA	10055	0.1386208800957649	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC	8258	0.11384696447845119	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG	7865	0.10842896289937254	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA	7748	0.10681597006285296	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.378626355999651E-5	0.0	0.0021230845882394623	0.0	0.0
2	1.378626355999651E-5	0.0	0.00232987854163941	0.0	0.0
3	4.1358790679989526E-5	1.378626355999651E-5	0.0024677411772393754	0.0	0.0
4	5.514505423998604E-5	2.757252711999302E-5	0.002591817549279344	0.0	0.0
5	5.514505423998604E-5	2.757252711999302E-5	0.002729680184879309	0.0	0.0
6	8.271758135997905E-5	2.757252711999302E-5	0.00292268787471926	0.0	0.0
7	9.650384491997557E-5	2.757252711999302E-5	0.0031019093009992146	0.0	0.0
8	1.2407637203996858E-4	4.1358790679989526E-5	0.003391420835759141	0.0	0.0
9	0.033459261660111525	5.514505423998604E-5	0.003736077424759054	0.0	0.0
10-11	0.08882489611705752	5.514505423998604E-5	0.004515001315898857	0.0	0.0
12-13	0.17640213538193533	5.514505423998604E-5	0.006355467501158391	0.0	0.0
14-15	0.19416573597899084	6.203818601998429E-5	0.011008331452657213	0.0	0.0
16-17	0.20227895208404878	8.271758135997905E-5	0.020403670068794837	0.0	0.0
18-19	0.2156240552101254	8.271758135997905E-5	0.048679296630347674	0.0	0.0
20-21	0.24427191088779815	8.96107131399773E-5	0.08626754422667815	0.0	0.0
22-23	0.31840065004989937	1.0339697669997383E-4	0.16529040695257816	0.0	0.0
24-25	0.3701473903223463	1.1029010847997208E-4	0.25426005883701563	0.0	0.0
26-27	0.38134872946484344	1.1718324025997033E-4	0.2776139893076497	0.0	0.0
28-29	0.39142648812720093	1.516488991599616E-4	0.28348693758420823	6.893131779998255E-6	0.0
30-31	0.3991605819843589	1.5854203093995986E-4	0.28454158674654795	1.378626355999651E-5	0.0
32-33	0.4092107681195964	1.654351627199581E-4	0.2851068235525078	1.378626355999651E-5	0.0
34-35	0.4161038998995946	1.792214262799546E-4	0.28528604497878773	1.378626355999651E-5	0.0
36-37	0.4243963374309325	1.8611455805995288E-4	0.2853342969012478	1.378626355999651E-5	0.0
38-39	0.43318508045043036	1.9300768983995114E-4	0.28538944195548777	1.378626355999651E-5	0.0
40-41	0.44202896852416806	2.136870851799459E-4	0.2854101213508278	1.378626355999651E-5	0.0
42-43	0.4527408953102854	2.2058021695994416E-4	0.28542390761438774	1.378626355999651E-5	0.0
44-45	0.46440407428204244	2.2058021695994416E-4	0.28544458700972775	2.757252711999302E-5	0.0
46-47	0.4774596658733591	2.4815274407993717E-4	0.2855135183275277	2.757252711999302E-5	0.0
48-49	0.4922178610143354	2.4815274407993717E-4	0.28554798398642767	2.757252711999302E-5	0.0
50-51	0.5086579803096312	2.619390076399337E-4	0.2855617702499877	2.757252711999302E-5	0.0
52-53	0.5269730314490866	2.6883213941993197E-4	0.2855686633817677	2.757252711999302E-5	0.0
54-55	0.5466804952081016	2.895115347599267E-4	0.2855686633817677	2.757252711999302E-5	0.0
56-57	0.568835020749016	2.895115347599267E-4	0.2855686633817677	2.757252711999302E-5	0.0
58-59	0.5940500968002496	2.9640466653992493E-4	0.2855824496453277	3.446565889999127E-5	0.0
60-61	0.6221740744626425	3.1708406187991973E-4	0.28559623590888766	4.1358790679989526E-5	0.0
62-63	0.6535033584027345	3.4465658899991274E-4	0.28562380843600765	4.1358790679989526E-5	0.0
64-65	0.687355528574306	3.4465658899991274E-4	0.2856307015677877	4.1358790679989526E-5	0.0
66-67	0.726811814883016	3.4465658899991274E-4	0.2856375946995677	4.1358790679989526E-5	0.0
68-69	0.7673778954083057	3.4465658899991274E-4	0.2856375946995677	4.1358790679989526E-5	0.0
70-71	0.8111699616066346	3.4465658899991274E-4	0.2856375946995677	4.1358790679989526E-5	0.0
72-73	0.8601318766399622	3.4465658899991274E-4	0.2856375946995677	4.1358790679989526E-5	0.0
74-75	0.9136639380434286	3.4465658899991274E-4	0.2856375946995677	4.8251922459987786E-5	0.0
76-77	0.9711733364839541	3.4465658899991274E-4	0.2856375946995677	5.514505423998604E-5	0.0
78-79	1.035238103247258	3.4465658899991274E-4	0.2856375946995677	5.514505423998604E-5	0.0
80-81	1.1033904971561008	3.4465658899991274E-4	0.2856375946995677	5.514505423998604E-5	0.0
82-83	1.1759131366134623	3.4465658899991274E-4	0.2856375946995677	6.203818601998429E-5	0.0
84-85	1.2550600757114023	3.584428525599092E-4	0.2856375946995677	6.893131779998255E-5	0.0
86-87	1.3416447039999602	3.584428525599092E-4	0.2856375946995677	6.893131779998255E-5	0.0
88-89	1.4349294563786765	3.584428525599092E-4	0.2856375946995677	6.893131779998255E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACG	23365	0.0	89.81009	1
TCGACGG	24790	0.0	84.609215	2
TATCTCG	25770	0.0	83.456856	2
CGACGGC	26255	0.0	79.97861	3
GACGGCG	26735	0.0	78.401566	4
ACGGCGC	26685	0.0	78.123245	5
ATCTCGA	27540	0.0	77.97229	3
ATATCTC	27880	0.0	77.583855	1
TCTCGAG	26425	0.0	76.56998	4
CGGCGCG	27315	0.0	76.2972	6
CTCGAGG	27230	0.0	73.767395	5
TCGAGGG	27420	0.0	73.12973	6
AGGGCGC	28370	0.0	70.69034	9
CGAGGGC	30460	0.0	65.871124	7
GAGGGCG	30900	0.0	64.887054	8
CTCGACG	1870	0.0	60.734592	5
TCTCGAC	2225	0.0	51.89723	4
CATATGT	19230	0.0	44.23251	4
ACATATG	20265	0.0	42.840427	3
CGCGCCG	55450	0.0	39.738796	9
>>END_MODULE