FastQCFastQC Report
Thu 26 May 2016
SRR2048267_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048267_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7253597
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT392060.5405042491332231No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT314070.4329851796288104No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161890.22318582077278348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG26200.076.937791
TCGACGG38450.057.82142
TATCTCG107550.056.4052542
ATATCTC118550.051.57341
ATCTCGA212900.048.7061541
TCTCGAG220500.045.4582752
CATATGT188400.043.613154
ACATATG202150.040.975573
CGACGGC54450.040.7353323
GACGGCG59350.037.3721774
TACATAT225550.037.19522
CTCGAGG272350.036.744253
ACGGCGC61350.036.540945
TCGAGGG275700.036.3666884
ATACATA237450.034.7998621
GTATACA134550.034.2132261
ATATGTC30300.034.1716655
TATACAT140100.033.0941122
CGGCGCG70300.031.9564176
CGAGGGC321950.031.1866575
Produced by FastQC (version 0.11.5)