##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048267_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7253597 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.258401590273074 34.0 31.0 34.0 30.0 34.0 2 32.412879292852914 34.0 31.0 34.0 30.0 34.0 3 32.48696998192759 34.0 31.0 34.0 30.0 34.0 4 35.95911076945686 37.0 35.0 37.0 35.0 37.0 5 35.92685118845174 37.0 35.0 37.0 35.0 37.0 6 35.86825515671742 37.0 35.0 37.0 35.0 37.0 7 35.80283285658136 37.0 35.0 37.0 35.0 37.0 8 35.76188296647856 37.0 35.0 37.0 33.0 37.0 9 37.3970630019837 39.0 37.0 39.0 34.0 39.0 10-11 37.38317995885352 39.0 37.0 39.0 34.0 39.0 12-13 37.40329715036554 39.0 37.0 39.0 34.0 39.0 14-15 38.799544556997034 40.0 38.0 41.0 35.0 41.0 16-17 38.84155392145442 40.0 38.0 41.0 35.0 41.0 18-19 38.92673200620327 40.0 38.0 41.0 35.0 41.0 20-21 39.00323467101909 40.0 39.0 41.0 35.5 41.0 22-23 39.01767268018888 40.0 39.0 41.0 36.0 41.0 24-25 39.02685481423906 40.0 39.0 41.0 36.0 41.0 26-27 38.92058009288357 40.0 38.0 41.0 35.5 41.0 28-29 38.77852374483997 40.0 38.0 41.0 35.0 41.0 30-31 38.65195012901874 40.0 38.0 41.0 35.0 41.0 32-33 38.54157495929261 40.0 38.0 41.0 35.0 41.0 34-35 38.47734069041884 40.0 38.0 41.0 35.0 41.0 36-37 38.402750180358794 40.0 38.0 41.0 34.5 41.0 38-39 38.29739872507392 40.0 38.0 41.0 34.0 41.0 40-41 38.15334736683055 40.0 38.0 41.0 34.0 41.0 42-43 37.9621988924943 40.0 37.0 41.0 33.5 41.0 44-45 37.723163487025815 40.0 37.0 41.0 33.0 41.0 46-47 37.41615849350329 40.0 37.0 41.0 33.0 41.0 48-49 37.12578366291923 40.0 37.0 41.0 32.0 41.0 50-51 36.87086393137088 40.0 36.5 41.0 31.5 41.0 52-53 36.661957991324854 40.0 36.0 41.0 31.0 41.0 54-55 36.435693973624396 39.0 35.5 41.0 31.0 41.0 56-57 36.1955292663764 39.0 35.0 41.0 30.5 41.0 58-59 35.951771169531476 39.0 35.0 41.0 30.0 41.0 60-61 35.69378489044814 39.0 35.0 40.5 30.0 41.0 62-63 35.39936661769326 38.0 35.0 40.0 29.0 41.0 64-65 35.077551454816145 37.5 35.0 40.0 29.0 41.0 66-67 34.77908608653058 37.0 34.5 40.0 29.0 41.0 68-69 34.438355067148066 36.5 34.0 39.0 28.0 41.0 70-71 34.115057812006924 36.0 34.0 39.0 28.0 41.0 72-73 33.76412854201853 35.5 34.0 39.0 28.0 40.5 74-75 33.42438006963993 35.0 34.0 37.5 27.0 40.0 76-77 33.10969860884193 35.0 34.0 37.0 27.0 39.0 78-79 32.80877873419215 35.0 33.0 36.5 26.5 39.0 80-81 32.537208849623156 35.0 33.0 36.0 26.0 38.5 82-83 32.27326380277261 35.0 33.0 36.0 26.0 37.0 84-85 32.00251826783318 35.0 33.0 35.0 26.0 37.0 86-87 31.809546353347173 35.0 33.0 35.0 25.0 36.5 88-89 31.649534224192493 35.0 33.0 35.0 25.0 36.0 90-91 31.480014260511027 35.0 33.0 35.0 25.0 36.0 92-93 31.260280037614443 35.0 33.0 35.0 24.0 36.0 94-95 31.103141034716984 35.0 33.0 35.0 23.5 35.5 96-97 30.963257677535708 35.0 33.0 35.0 22.5 35.0 98-99 30.81654391607364 35.0 33.0 35.0 19.5 35.0 100-101 29.955056711863094 34.5 31.5 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.49402392674811324 1101 2 0.42735766375861317 1101 3 0.4363189965423828 1101 4 0.30962703966124394 1101 5 0.3073307665890539 1101 6 0.28416971511631317 1101 7 0.26438624462996074 1101 8 0.25852724589195475 1101 9 0.3050576596019212 1101 10-11 0.29689960762978984 1101 12-13 0.3267373361853174 1101 14-15 0.4546247724328367 1101 16-17 0.4863127223672592 1101 18-19 0.4823582595286524 1101 20-21 0.4822898575244352 1101 22-23 0.5113469659329013 1101 24-25 0.491771389225903 1101 26-27 0.4949364597545838 1101 28-29 0.5355339081462986 1101 30-31 0.5254854031184664 1101 32-33 0.5285039637363056 1101 34-35 0.5365033289045584 1101 36-37 0.5194743484485045 1101 38-39 0.5225217933070851 1101 40-41 0.5570991425699034 1101 42-43 0.5682988142118361 1101 44-45 0.48498885346880627 1101 46-47 0.4662050267168283 1101 48-49 0.4213733554878445 1101 50-51 0.41906910746228476 1101 52-53 0.40816617498764884 1101 54-55 0.4520572175417996 1101 56-57 0.44259286950432397 1101 58-59 0.44840632770677047 1101 60-61 0.4678191701253098 1101 62-63 0.4492697329921924 1101 64-65 0.4464091119040532 1101 66-67 0.4162897113905615 1101 68-69 0.39609527665156463 1101 70-71 0.37784501557683825 1101 72-73 0.34888939472644154 1101 74-75 0.3749678632274751 1101 76-77 0.3647170894514602 1101 78-79 0.37085035086535356 1101 80-81 0.38288478329555176 1101 82-83 0.40314044926140724 1101 84-85 0.4168797144862282 1101 86-87 0.40794366674680305 1101 88-89 0.39699129375054554 1101 90-91 0.40154394877523103 1101 92-93 0.47149350144160707 1101 94-95 0.42600148775569124 1101 96-97 0.40664684536403684 1101 98-99 0.42465817478090884 1101 100-101 0.5558632427517267 1102 1 0.4543034097360561 1102 2 0.42576598455220704 1102 3 0.3942285016752436 1102 4 0.2562410363034857 1102 5 0.2649278550504732 1102 6 0.19769454177486523 1102 7 0.19053175197283423 1102 8 0.19658428849857046 1102 9 0.26184050128563996 1102 10-11 0.26965179205610923 1102 12-13 0.27295857425331604 1102 14-15 0.4042664244471581 1102 16-17 0.41928801329807186 1102 18-19 0.4383613588637303 1102 20-21 0.4740220044058958 1102 22-23 0.46674768145948065 1102 24-25 0.4527472408897779 1102 26-27 0.4680341937664352 1102 28-29 0.4432590311387017 1102 30-31 0.4821221423661868 1102 32-33 0.4711786084814449 1102 34-35 0.4960739552119193 1102 36-37 0.5126150411963835 1102 38-39 0.5172488957565093 1102 40-41 0.5185291100563347 1102 42-43 0.5083346641692543 1102 44-45 0.47049865352069276 1102 46-47 0.42510491002256146 1102 48-49 0.40808647945677734 1102 50-51 0.47758046223466266 1102 52-53 0.4785949841936059 1102 54-55 0.5048133346068084 1102 56-57 0.45693227499156563 1102 58-59 0.4478527409569395 1102 60-61 0.4768427279881564 1102 62-63 0.5147845484577402 1102 64-65 0.47382865558802933 1102 66-67 0.42790124580252353 1102 68-69 0.41364474900088055 1102 70-71 0.39678794712799004 1102 72-73 0.3932500355964095 1102 74-75 0.3756401221140635 1102 76-77 0.3587854316089292 1102 78-79 0.3853469030932928 1102 80-81 0.3642704378554029 1102 82-83 0.3528508213938437 1102 84-85 0.3850055623892601 1102 86-87 0.3554995332432469 1102 88-89 0.35659512515699276 1102 90-91 0.35618607215758047 1102 92-93 0.4416193790848588 1102 94-95 0.4276614539702237 1102 96-97 0.3537371186232363 1102 98-99 0.3599323413437503 1102 100-101 0.43317603204213384 1103 1 0.41442078727213527 1103 2 0.34528595427860154 1103 3 0.31756981614203283 1103 4 0.244003987743028 1103 5 0.2335100582467149 1103 6 0.22570255093788205 1103 7 0.22232903102882062 1103 8 0.21017113076938188 1103 9 0.27847916538360096 1103 10-11 0.26548726748095675 1103 12-13 0.2564502812647049 1103 14-15 0.3517029006324819 1103 16-17 0.32086345198260346 1103 18-19 0.35065502846878616 1103 20-21 0.36870096704770106 1103 22-23 0.37886367218431616 1103 24-25 0.3970230668681012 1103 26-27 0.3843387967510239 1103 28-29 0.37634177648400424 1103 30-31 0.3994752300476918 1103 32-33 0.3908922208973564 1103 34-35 0.39477581883207336 1103 36-37 0.4267723277930102 1103 38-39 0.4311339615455978 1103 40-41 0.3430065482195488 1103 42-43 0.30825310677114715 1103 44-45 0.3095522379290685 1103 46-47 0.3289025624928783 1103 48-49 0.3125936380684564 1103 50-51 0.3647784872737887 1103 52-53 0.342815203744145 1103 54-55 0.3461784112983963 1103 56-57 0.33107819804814653 1103 58-59 0.34773693077668355 1103 60-61 0.39847160841634377 1103 62-63 0.4206005711711924 1103 64-65 0.3804775358929646 1103 66-67 0.33582875572190574 1103 68-69 0.3468592378383448 1103 70-71 0.3131107907102262 1103 72-73 0.33368264284002436 1103 74-75 0.30378243655049886 1103 76-77 0.31083421301396186 1103 78-79 0.2938695907265796 1103 80-81 0.3291936686536161 1103 82-83 0.32417386236785006 1103 84-85 0.3215773465392999 1103 86-87 0.30120572389702716 1103 88-89 0.34750441406014687 1103 90-91 0.2924740044242533 1103 92-93 0.3144692627204684 1103 94-95 0.3048643203132002 1103 96-97 0.3261693656610589 1103 98-99 0.3263377560996048 1103 100-101 0.44971669433578043 1104 1 0.3474000646365667 1104 2 0.32922182371282815 1104 3 0.30315024166550586 1104 4 0.24599457507870426 1104 5 0.2183530378410623 1104 6 0.19443336334467887 1104 7 0.23501251451616412 1104 8 0.19646924297204293 1104 9 0.22004764207669325 1104 10-11 0.2199854153480132 1104 12-13 0.2232651446333307 1104 14-15 0.2772491391625209 1104 16-17 0.3354164537617734 1104 18-19 0.31857881864760884 1104 20-21 0.31177390465329324 1104 22-23 0.3262763485181637 1104 24-25 0.2958942830590132 1104 26-27 0.31414252525865294 1104 28-29 0.3181279889402049 1104 30-31 0.31567415640126484 1104 32-33 0.25552966379625985 1104 34-35 0.3032089061220091 1104 36-37 0.27285049371010217 1104 38-39 0.2645376159315944 1104 40-41 0.2710524016885856 1104 42-43 0.26147852853338094 1104 44-45 0.2522473060621735 1104 46-47 0.28449029684420424 1104 48-49 0.2582190099507997 1104 50-51 0.3136025535654383 1104 52-53 0.31275544981732395 1104 54-55 0.34702578987431565 1104 56-57 0.3090681391236956 1104 58-59 0.3296470232569533 1104 60-61 0.29054410657063556 1104 62-63 0.23733321371742022 1104 64-65 0.28020409021630854 1104 66-67 0.25451266958386043 1104 68-69 0.2508581190319106 1104 70-71 0.26532999186959216 1104 72-73 0.2651788942380051 1104 74-75 0.27430299713228834 1104 76-77 0.22054697356551145 1104 78-79 0.24965812932826736 1104 80-81 0.26708797216658553 1104 82-83 0.3019833519187003 1104 84-85 0.26008673525512904 1104 86-87 0.2714969584842848 1104 88-89 0.2259317188021086 1104 90-91 0.21494240812681653 1104 92-93 0.29428693441613163 1104 94-95 0.28044293468846604 1104 96-97 0.33066351280365325 1104 98-99 0.3390966459118303 1104 100-101 0.4784005621160965 1105 1 0.3005268899210378 1105 2 0.29328548896125994 1105 3 0.20483502559534372 1105 4 0.15766106514646339 1105 5 0.1588272183834647 1105 6 0.15346217055106592 1105 7 0.19597538585166774 1105 8 0.1522577141797612 1105 9 0.20246624101586974 1105 10-11 0.17010857849040661 1105 12-13 0.1963986040756538 1105 14-15 0.24372959910826353 1105 16-17 0.24006677516275943 1105 18-19 0.2544223300531101 1105 20-21 0.2695172180069818 1105 22-23 0.30221916384680725 1105 24-25 0.2852736030699816 1105 26-27 0.26935793990077883 1105 28-29 0.25521802948429695 1105 30-31 0.2297304791029049 1105 32-33 0.27140344694034724 1105 34-35 0.27193420345433594 1105 36-37 0.33883816250289556 1105 38-39 0.2936392503243255 1105 40-41 0.2660618381260278 1105 42-43 0.24960710969791222 1105 44-45 0.27644950496485876 1105 46-47 0.27092071888312574 1105 48-49 0.2626858137182069 1105 50-51 0.3032094825604048 1105 52-53 0.29458597108006046 1105 54-55 0.29106645198817915 1105 56-57 0.2968787046247172 1105 58-59 0.22957249576628413 1105 60-61 0.23640388928004086 1105 62-63 0.23573095240095654 1105 64-65 0.2685072105191537 1105 66-67 0.20841623579870827 1105 68-69 0.20290565021196016 1105 70-71 0.20254272143001373 1105 72-73 0.19089444182053228 1105 74-75 0.23018856688881328 1105 76-77 0.19963433149582244 1105 78-79 0.2541997871115882 1105 80-81 0.2341568087872048 1105 82-83 0.2322770445832063 1105 84-85 0.20693647324091557 1105 86-87 0.22113920403300114 1105 88-89 0.19063153402773025 1105 90-91 0.20665382560151002 1105 92-93 0.21593387293006927 1105 94-95 0.21289485011929798 1105 96-97 0.21896508495915157 1105 98-99 0.2583123713604394 1105 100-101 0.38962988327892134 1106 1 0.32747301025639075 1106 2 0.3066488099216471 1106 3 0.2541279164114485 1106 4 0.18703849675539175 1106 5 0.209604587640996 1106 6 0.17837818119311777 1106 7 0.19002990292813138 1106 8 0.20713768467525995 1106 9 0.22599791063925778 1106 10-11 0.23388156698920426 1106 12-13 0.2520481614487835 1106 14-15 0.27549832323771994 1106 16-17 0.24417403469222876 1106 18-19 0.2841085217335717 1106 20-21 0.2601340925081459 1106 22-23 0.2624523478694556 1106 24-25 0.261881238544845 1106 26-27 0.28754300905068675 1106 28-29 0.2659815937206744 1106 30-31 0.2690384465767863 1106 32-33 0.19881513748105561 1106 34-35 0.23212405495389277 1106 36-37 0.2799339034853716 1106 38-39 0.28531100376383023 1106 40-41 0.21096955999703226 1106 42-43 0.18209613426063243 1106 44-45 0.11844969602270794 1106 46-47 0.03931232146750574 1106 48-49 0.07583436254179077 1106 50-51 0.14143329312270936 1106 52-53 0.11816370572872614 1106 54-55 0.060474281914352446 1106 56-57 0.07877084631691389 1106 58-59 0.08864455106126456 1106 60-61 0.09950817346953755 1106 62-63 0.18202034888275875 1106 64-65 0.15104463740151886 1106 66-67 0.11821562785590345 1106 68-69 0.08964241597185207 1106 70-71 0.13066027668004665 1106 72-73 0.112446635432633 1106 74-75 0.07677077388665055 1106 76-77 0.08178935293622658 1106 78-79 0.1458623226568676 1106 80-81 0.13389602957083468 1106 82-83 0.13127328371676583 1106 84-85 0.179986215220012 1106 86-87 0.16990228911271998 1106 88-89 0.16842656755380148 1106 90-91 0.22476744498002077 1106 92-93 0.26796182822014103 1106 94-95 0.30678336723517674 1106 96-97 0.24841190202371877 1106 98-99 0.28203804139591426 1106 100-101 0.40648802292896136 1107 1 0.17911842284350143 1107 2 0.19901589436529576 1107 3 0.17475732599877603 1107 4 0.13401395818458184 1107 5 0.14751677922935613 1107 6 0.1324673284576079 1107 7 0.055716213587174934 1107 8 0.0855581989988039 1107 9 0.06403330818522335 1107 10-11 0.1092919712697622 1107 12-13 0.05733706842926978 1107 14-15 0.0997891928262078 1107 16-17 0.13356400990927142 1107 18-19 0.12056393918005881 1107 20-21 0.13654881974289168 1107 22-23 0.1684620509911241 1107 24-25 0.17882115598667525 1107 26-27 0.12579713902801615 1107 28-29 0.1692649562846711 1107 30-31 0.11317818333773033 1107 32-33 0.19566859845793516 1107 34-35 0.19926685169054537 1107 36-37 0.20453642630678814 1107 38-39 0.1668077170553488 1107 40-41 0.14529409888558575 1107 42-43 0.13153762217643816 1107 44-45 0.13122014340885357 1107 46-47 0.10322887965615735 1107 48-49 0.04828944834775939 1107 50-51 0.08783263475795167 1107 52-53 0.06746237051071802 1107 54-55 0.017937098142994046 1107 56-57 0.04451781458023163 1107 58-59 0.059289698559418014 1107 60-61 0.09198110232502898 1107 62-63 0.10088690557520863 1107 64-65 0.20399674963359615 1107 66-67 0.16148859322048992 1107 68-69 0.155312093975013 1107 70-71 0.11997318423205172 1107 72-73 0.17123110620603654 1107 74-75 0.12854421881712597 1107 76-77 0.152445820486939 1107 78-79 0.1483620264744303 1107 80-81 0.18892455701112 1107 82-83 0.22005916722714147 1107 84-85 0.1904908669690002 1107 86-87 0.17530088834525515 1107 88-89 0.2292203301978617 1107 90-91 0.20330822875154908 1107 92-93 0.21640059935825207 1107 94-95 0.22924853616321883 1107 96-97 0.24986620317755026 1107 98-99 0.2385698688480069 1107 100-101 0.26622863485356874 1108 1 0.1741271545745704 1108 2 0.12565519557180238 1108 3 0.15401474261225445 1108 4 0.11294788127006683 1108 5 0.09996285759390844 1108 6 0.053455878153329195 1108 7 0.0546222632264346 1108 8 0.08555458748240596 1108 9 0.11958300844970893 1108 10-11 0.08916774561787832 1108 12-13 0.1028535691933854 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84-85 -0.3444819487261519 2214 86-87 -0.31521766855619404 2214 88-89 -0.31582422258178866 2214 90-91 -0.37256322083261395 2214 92-93 -0.3141549208492158 2214 94-95 -0.3146257858735595 2214 96-97 -0.35243795892527174 2214 98-99 -0.3836440365419911 2214 100-101 -0.4757895277208348 2215 1 -0.5830279862214667 2215 2 -0.5368128567818928 2215 3 -0.45821153617274035 2215 4 -0.3460499163069102 2215 5 -0.34803317514600707 2215 6 -0.3515015941961366 2215 7 -0.33371898759511964 2215 8 -0.30672887068986654 2215 9 -0.3992868505411735 2215 10-11 -0.40229070399063716 2215 12-13 -0.44721947850888455 2215 14-15 -0.5592557270611422 2215 16-17 -0.5567393742028344 2215 18-19 -0.5805217168022807 2215 20-21 -0.5455654679999924 2215 22-23 -0.5481872599059372 2215 24-25 -0.5475204318558724 2215 26-27 -0.5476057357185553 2215 28-29 -0.49705492369755433 2215 30-31 -0.5675956341689883 2215 32-33 -0.5638188935544761 2215 34-35 -0.6261012352565984 2215 36-37 -0.5946093279723357 2215 38-39 -0.5635036743777349 2215 40-41 -0.5046837462115477 2215 42-43 -0.5062004608525115 2215 44-45 -0.456392307515074 2215 46-47 -0.5045396022662345 2215 48-49 -0.49348273350914695 2215 50-51 -0.505227161127678 2215 52-53 -0.5043878860128146 2215 54-55 -0.4611603755406577 2215 56-57 -0.45847013893785515 2215 58-59 -0.43520674745230536 2215 60-61 -0.5089351376789679 2215 62-63 -0.4780190629687695 2215 64-65 -0.4939589510793141 2215 66-67 -0.47124327065768057 2215 68-69 -0.35609143555740275 2215 70-71 -0.43242907780325623 2215 72-73 -0.38741430227251783 2215 74-75 -0.40840220444802355 2215 76-77 -0.4176547246419986 2215 78-79 -0.3776712992328797 2215 80-81 -0.4207405686735797 2215 82-83 -0.37736652509713764 2215 84-85 -0.3930511957905196 2215 86-87 -0.43921464901303864 2215 88-89 -0.4195685789394261 2215 90-91 -0.4267864127860683 2215 92-93 -0.3837984003183017 2215 94-95 -0.42941442530960217 2215 96-97 -0.4785597205569907 2215 98-99 -0.5060603612211274 2215 100-101 -0.5780316017634206 2216 1 -0.6298113679677968 2216 2 -0.6142039628408646 2216 3 -0.610751411337688 2216 4 -0.4524308620207407 2216 5 -0.4773228602552706 2216 6 -0.46126178720068367 2216 7 -0.3903455314773936 2216 8 -0.3622082107271467 2216 9 -0.5224543227990566 2216 10-11 -0.5196916171984327 2216 12-13 -0.4952033663718254 2216 14-15 -0.6877441494793288 2216 16-17 -0.6364717197794789 2216 18-19 -0.6862640908577049 2216 20-21 -0.6604869947799017 2216 22-23 -0.7539660259497794 2216 24-25 -0.6730182329605299 2216 26-27 -0.6863607922573962 2216 28-29 -0.6762132166267705 2216 30-31 -0.6496169781779813 2216 32-33 -0.7036311011089751 2216 34-35 -0.7155500987601258 2216 36-37 -0.7646591068819859 2216 38-39 -0.7464786020860927 2216 40-41 -0.7321434814236625 2216 42-43 -0.6804959093763614 2216 44-45 -0.6091409682951507 2216 46-47 -0.6150694262318481 2216 48-49 -0.5557459584067814 2216 50-51 -0.48120833427496024 2216 52-53 -0.5318782973287384 2216 54-55 -0.575768215645482 2216 56-57 -0.5520460453884084 2216 58-59 -0.5469314951359863 2216 60-61 -0.5565142080956136 2216 62-63 -0.5866904369238881 2216 64-65 -0.5248886700388553 2216 66-67 -0.5095132656335792 2216 68-69 -0.505457914028824 2216 70-71 -0.47061281508720754 2216 72-73 -0.5390199341379827 2216 74-75 -0.5222974970378118 2216 76-77 -0.5917554370990388 2216 78-79 -0.6009664227097815 2216 80-81 -0.6019994994640214 2216 82-83 -0.5664819711208402 2216 84-85 -0.5049885605304532 2216 86-87 -0.4584545880535842 2216 88-89 -0.5027945397657234 2216 90-91 -0.5653584928780226 2216 92-93 -0.4851083862345682 2216 94-95 -0.6006707088311529 2216 96-97 -0.5292594578776324 2216 98-99 -0.6519265808580315 2216 100-101 -0.8141994039608846 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 359.0 3 82.0 4 105.0 5 134.0 6 320.0 7 758.0 8 1739.0 9 3166.0 10 5156.0 11 6182.0 12 7008.0 13 10459.0 14 17738.0 15 31759.0 16 44652.0 17 62396.0 18 62219.0 19 40794.0 20 32147.0 21 28451.0 22 26190.0 23 25537.0 24 27304.0 25 31191.0 26 38247.0 27 48356.0 28 60726.0 29 78046.0 30 102363.0 31 136548.0 32 183881.0 33 251824.0 34 355560.0 35 516448.0 36 902229.0 37 1684822.0 38 1940262.0 39 487833.0 40 606.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 23.733915026874197 38.53386016125624 16.718236844696012 21.01398796717355 2 22.72358862549936 28.24409166095353 31.520904012138505 17.511415701408602 3 21.943975328078924 29.81552582927902 23.840760230451405 24.399738612190653 4 22.928596667281074 26.128540088455427 27.28425634895349 23.65860689531001 5 22.750001895611764 28.79510091203052 24.903182490889 23.551714701468722 6 24.12989031510849 25.4288182814678 27.184595449678277 23.25669595374543 7 23.278588540278704 29.290516139785545 24.303431800801725 23.12746351913402 8 24.523450126372726 25.38973023252223 27.782753890064484 22.30406575104056 9 23.952971194843055 26.61741478055646 25.96052689444975 23.469087130150736 10-11 24.025493834300416 26.195844627155328 26.362479194805005 23.41618234373925 12-13 23.80577525881297 26.478835259251376 25.81976914350218 23.89562033843347 14-15 23.2570920685409 26.461847337513696 26.208428205816816 24.072632388128593 16-17 23.391387748726597 26.57606288300825 26.240491441694374 23.792057926570777 18-19 22.876953323985326 26.717241114994394 27.21586958856413 23.189935972456148 20-21 21.955959634087673 26.49970624912845 28.248356280688657 23.295977836095215 22-23 21.59914444501047 26.177547926954936 29.109349967225278 23.113957660809312 24-25 21.62474944204995 25.829918171921477 30.16999304410668 22.375339341921897 26-27 21.52156892915496 25.74073771514003 30.831913732969536 21.905779622735473 28-29 21.680779733438488 25.796082443381703 30.842013392189294 21.68112443099052 30-31 21.564089639685346 25.73905147730331 30.729093136065437 21.967765746945904 32-33 21.673257328925114 25.66547743625019 30.750793695325633 21.910471539499067 34-35 21.747410592547965 25.815423810830225 30.598724217938976 21.838441378682834 36-37 21.653953204182695 25.73786495169224 30.513330145030114 22.094851699094946 38-39 21.67918895962927 25.735817691553585 30.536801258741008 22.04819209007614 40-41 21.811199326348017 26.021648293942988 30.22906428355477 21.938088096154225 42-43 21.956658193169538 25.973527340986823 29.85036251669344 22.219451949150194 44-45 21.91271447807205 26.15914559355862 29.62082812155128 22.307311806818053 46-47 22.186047832544322 26.428536076652726 28.860419182372553 22.5249969084304 48-49 22.361325746194247 26.463128846327898 28.22378792957777 22.951757477900085 50-51 22.463730753169774 26.54028752907006 27.976506001091593 23.019475716668573 52-53 22.593614911797147 26.61514058392811 27.764085879623135 23.027158624651605 54-55 22.654267415465448 26.539041373039794 27.477836240822402 23.328854970672356 56-57 22.720417098001445 26.45888928327619 27.63209460258272 23.188599016139648 58-59 22.823661395179286 26.839356943293314 27.234254904376236 23.102726757151164 60-61 22.806180679342017 26.65887224115836 27.08643974691026 23.44850733258937 62-63 22.77042761215271 26.72235248069727 27.290223978323837 23.216995928826183 64-65 22.832802596176336 26.76813641464872 27.22442768171156 23.174633307463385 66-67 22.89667176967147 26.610980979778297 27.143601505438113 23.34874574511211 68-69 22.882666040400636 26.66280971716906 27.239814968442793 23.214709273987506 70-71 22.985389962880372 26.847795742099585 27.036978317588222 23.129835977431817 72-73 22.97877854997098 26.711123304441575 26.874395982083154 23.435702163504292 74-75 22.9335772233254 26.745286631285797 27.079033854281015 23.242102291107788 76-77 22.866859238176446 26.986513216055652 26.942410870766274 23.20421667500163 78-79 22.887642535988395 26.96121703134713 26.900894586475886 23.250245846188587 80-81 22.848399288894523 26.923035033456284 27.028170557779386 23.200395119869803 82-83 22.903072400862772 27.149132750665895 26.816938829431397 23.13085601903994 84-85 22.8787727237853 27.06930285335819 26.80444521313347 23.24747920972304 86-87 22.831390470801637 27.144512891167093 26.839870171133352 23.18422646689792 88-89 22.833808950368798 27.356689476568956 26.593234346808813 23.216267226253425 90-91 22.830545854806452 27.30517578602952 26.533446364619095 23.33083199454494 92-93 22.784018796031567 27.23466197997476 26.760694158335223 23.22062506565845 94-95 22.859274273433137 27.378249048410865 26.578788713089168 23.18368796506683 96-97 22.871563899302792 27.28357737328027 26.44543123221435 23.399427495202588 98-99 22.84472989226131 27.17559829246006 26.658687503756816 23.320984311521816 100-101 22.9564600593786 27.26639925387281 26.409952222768325 23.367188463980266 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 362.0 1 279.0 2 157.0 3 107.0 4 93.5 5 79.5 6 65.0 7 69.0 8 85.0 9 85.0 10 82.5 11 90.0 12 117.0 13 303.5 14 494.0 15 694.0 16 3095.0 17 3675.5 18 2218.5 19 2646.5 20 3649.5 21 5471.0 22 8092.0 23 11780.5 24 17064.0 25 23789.5 26 31447.5 27 41039.0 28 52297.5 29 64306.0 30 77688.0 31 93731.0 32 113171.0 33 131934.0 34 146308.5 35 163090.5 36 183265.5 37 206434.5 38 226663.0 39 239324.5 40 249880.5 41 262748.5 42 280566.5 43 295369.0 44 301427.0 45 300539.5 46 297837.5 47 294246.0 48 291573.5 49 284312.5 50 273811.5 51 262568.5 52 237520.0 53 207591.0 54 187738.5 55 172487.0 56 155425.0 57 138328.0 58 126408.5 59 117380.5 60 103896.0 61 90218.5 62 80106.0 63 71465.5 64 62624.0 65 52947.0 66 44171.5 67 36325.5 68 29093.5 69 23060.0 70 17966.0 71 13472.0 72 10042.0 73 7707.0 74 5870.5 75 4166.0 76 2790.0 77 1902.0 78 1337.5 79 820.5 80 366.5 81 153.5 82 73.0 83 38.0 84 23.5 85 15.0 86 10.5 87 5.0 88 2.5 89 1.5 90 1.0 91 0.5 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2947641011762854 2 0.017991073945795445 3 9.650384491997557E-5 4 0.0 5 2.757252711999302E-5 6 0.0 7 0.0 8 7.444582322398115E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 1.378626355999651E-5 16-17 0.0 18-19 0.0 20-21 2.0679395339994763E-5 22-23 0.004046268354858975 24-25 4.894123563798761E-4 26-27 0.009312621034777641 28-29 0.01188375918871699 30-31 0.00525256641635867 32-33 3.653359843399075E-4 34-35 9.650384491997557E-5 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 8.96107131399773E-5 50-51 0.0 52-53 0.0016336722318595863 54-55 0.002784825239119295 56-57 0.005976345253258486 58-59 0.006307215578698403 60-61 0.0016888172860995724 62-63 0.00390840571925901 64-65 8.96107131399773E-5 66-67 3.79122247899904E-4 68-69 3.23977193659918E-4 70-71 3.032977983199232E-4 72-73 0.0011994049297196962 74-75 7.375651004598132E-4 76-77 1.999008216199494E-4 78-79 0.0010753285576797277 80-81 0.0014061988831196438 82-83 3.446565889999127E-5 84-85 0.00367403923873907 86-87 0.007037887547378219 88-89 0.005342177129498647 90-91 0.0020403670068794835 92-93 0.002081725797559473 94-95 0.0028123977662392876 96-97 0.0028675428204792738 98-99 0.0016681378907595775 100-101 0.0019576494255195043 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7253597.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.84070541168418 #Duplication Level Percentage of deduplicated Percentage of total 1 80.81833980005162 42.70498085235919 2 11.362828454531032 12.00839742018754 3 3.114584582130047 4.93730539152322 4 1.238661491477308 2.618069879037992 5 0.6485778171095584 1.713565468521968 6 0.4166181193879507 1.3208637189449144 7 0.2942367305367255 1.0883373479711749 8 0.22737589759536542 0.9611762258043182 9 0.17979286644719275 0.8550343701952552 >10 1.4384340002923273 14.796766020526894 >50 0.15352494048845583 5.801063402515374 >100 0.10519820392761242 8.709457698445608 >500 0.001044084521751171 0.364064483760212 >1k 6.003088202632778E-4 0.6044180399076696 >5k 1.0440153299496718E-4 0.31935890610044004 >10k+ 7.83011497462254E-5 1.1971407741980653 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 39206 0.5405042491332231 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 31407 0.4329851796288104 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16189 0.22318582077278348 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.378626355999651E-5 9.098933949597696E-4 0.0 0.0 0.0 2 1.378626355999651E-5 0.0010201835034397417 0.0 0.0 0.0 3 4.1358790679989526E-5 0.0010891148212397242 0.0 0.0 0.0 4 5.514505423998604E-5 0.0011718324025997034 0.0 0.0 0.0 5 5.514505423998604E-5 0.0012821225110796754 0.0 0.0 0.0 6 8.271758135997905E-5 0.0013924126195596475 0.0 0.0 0.0 7 9.650384491997557E-5 0.0015440615187196092 0.0 0.0 0.0 8 1.2407637203996858E-4 0.0017508554721195567 0.0 0.0 0.0 9 0.034410513845751285 0.0020265807433194867 0.0 0.0 0.0 10-11 0.09041031642645711 0.0027365733166593072 0.0 0.0 0.0 12-13 0.17874580018713473 0.004473642525218867 0.0 0.0 0.0 14-15 0.19681269858251016 0.00916097213561768 0.0 0.0 0.0 16-17 0.20491902155578812 0.018721745914475257 0.0 0.0 0.0 18-19 0.21818830023228475 0.04697669308068811 0.0 0.0 0.0 20-21 0.24662246882477756 0.08456494067701859 0.0 0.0 0.0 22-23 0.319910245909719 0.16320178802323868 0.0 0.0 0.0 24-25 0.3708022378414461 0.2518612489775762 0.0 0.0 0.0 26-27 0.3818863937436833 0.2753944008744903 0.0 0.0 0.0 28-29 0.39189522108824076 0.28144657057732875 0.0 0.0 0.0 30-31 0.39950523857335885 0.2827838381426484 0.0 0.0 0.0 32-33 0.4093348444916364 0.28365926587870816 0.0 0.0 0.0 34-35 0.41602807545001463 0.2840659606537281 0.0 0.0 1.378626355999651E-5 36-37 0.4237897418342927 0.284383044715608 0.0 0.0 1.378626355999651E-5 38-39 0.4321511106834306 0.2846105180643479 0.0 0.0 1.378626355999651E-5 40-41 0.44091917430758837 0.2847208081728279 0.0 0.0 1.378626355999651E-5 42-43 0.45163110109370563 0.2847828463588479 0.0 0.0 1.378626355999651E-5 44-45 0.4630599135849428 0.2848655639402079 0.0 0.0 1.378626355999651E-5 46-47 0.47575016919191954 0.28493449525800785 0.0 0.0 1.378626355999651E-5 48-49 0.4902877841159359 0.28497585404868786 0.0 0.0 1.378626355999651E-5 50-51 0.5067141171476717 0.2849896403122478 0.0 0.0 1.378626355999651E-5 52-53 0.525015382023567 0.2850241059711478 0.0 0.0 1.378626355999651E-5 54-55 0.5448124564957221 0.28503099910292784 0.0 0.0 1.378626355999651E-5 56-57 0.5670083408273164 0.2850378922347078 0.0 0.0 1.378626355999651E-5 58-59 0.5918718671577701 0.28505857163004783 0.0 0.0 1.378626355999651E-5 60-61 0.619954486029483 0.2850723578936078 0.0 0.0 1.378626355999651E-5 62-63 0.6513733806827151 0.2850999304207278 0.0 0.0 1.378626355999651E-5 64-65 0.6852393371178465 0.2851068235525078 0.0 0.0 1.378626355999651E-5 66-67 0.7245301882638366 0.2851137166842878 0.0 0.0 1.378626355999651E-5 68-69 0.7648619023086063 0.2851137166842878 0.0 0.0 1.378626355999651E-5 70-71 0.8086953272976152 0.2851137166842878 0.0 0.0 1.378626355999651E-5 72-73 0.8576158835402629 0.2851137166842878 0.0 0.0 1.378626355999651E-5 74-75 0.9109480441221094 0.2851206098160678 0.0 0.0 1.378626355999651E-5 76-77 0.9683885112448347 0.2851412892114078 0.0 0.0 1.378626355999651E-5 78-79 1.0322602703182988 0.2851550754749678 0.0 0.0 1.378626355999651E-5 80-81 1.1005987787852014 0.2851550754749678 0.0 0.0 1.378626355999651E-5 82-83 1.173024914397643 0.2851550754749678 0.0 0.0 1.378626355999651E-5 84-85 1.2521029221777829 0.2851550754749678 0.0 0.0 1.378626355999651E-5 86-87 1.338597939753201 0.2851826480020878 0.0 0.0 1.378626355999651E-5 88-89 1.4317379363645375 0.2851964342656478 0.0 0.0 1.378626355999651E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 2620 0.0 76.93779 1 TCGACGG 3845 0.0 57.8214 2 TATCTCG 10755 0.0 56.405254 2 ATATCTC 11855 0.0 51.5734 1 ATCTCGA 21290 0.0 48.706154 1 TCTCGAG 22050 0.0 45.458275 2 CATATGT 18840 0.0 43.61315 4 ACATATG 20215 0.0 40.97557 3 CGACGGC 5445 0.0 40.735332 3 GACGGCG 5935 0.0 37.372177 4 TACATAT 22555 0.0 37.1952 2 CTCGAGG 27235 0.0 36.74425 3 ACGGCGC 6135 0.0 36.54094 5 TCGAGGG 27570 0.0 36.366688 4 ATACATA 23745 0.0 34.799862 1 GTATACA 13455 0.0 34.213226 1 ATATGTC 3030 0.0 34.171665 5 TATACAT 14010 0.0 33.094112 2 CGGCGCG 7030 0.0 31.956417 6 CGAGGGC 32195 0.0 31.186657 5 >>END_MODULE