FastQCFastQC Report
Tue 31 May 2016
SRR2048266_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048266_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4442212
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT74470.16764170642913936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG317850.094.2668841
TCGACGG321300.093.210322
ACGGCGC329950.090.616735
CGACGGC330900.090.4651953
GACGGCG332950.089.873594
CGGCGCG336500.088.8105246
CGCGCCG370600.081.164269
GGCGCGC383900.078.352377
GCGCGCC385800.078.028068
TATCTCG29650.074.9859162
ATCTCGA33400.065.9889763
ATATCTC34850.063.524561
TCTCGAG39550.055.243594
CTCGAGG43700.049.6701555
AGGGCGC48500.045.048149
ATCCATA344050.043.45371616-17
TCCATAT345550.043.26533518-19
CCATATT349250.042.75257518-19
CGAGGGC51400.042.2292987
CATATTT356050.041.94940620-21
Produced by FastQC (version 0.11.5)