FastQCFastQC Report
Tue 31 May 2016
SRR2048266_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048266_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4442212
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG24400.086.507651
TCGACGG27650.077.476692
ACGGCGC37350.056.8468785
CGACGGC38000.056.1244933
GACGGCG39500.053.6324274
CGGCGCG45900.046.257756
ATCTCGA20700.037.664731
CGCGCCG85600.035.3472339
GGCGCGC97200.031.226577
GCGCGCC100850.030.0964098
TCTCGAG29400.027.4657382
TATCTCG10100.021.6334692
CTCGAGG41700.021.5470141
ATCCATA52750.021.20596916-17
GGGCGCG42750.020.88876
GCCGGAT80250.020.50916112-13
TCCATAT52750.020.3955518-19
CCATATT55200.020.30778718-19
CGGATCC82900.019.96815714-15
CGCCGGA79100.019.93661110-11