FastQCFastQC Report
Tue 31 May 2016
SRR2048265_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048265_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6806677
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT327470.4811011305516627No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA79530.1168411546485899No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC79470.11675300590875694No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC73290.10767368570596195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG536050.094.598151
TCGACGG541950.093.2187652
CGACGGC563000.089.804843
ACGGCGC561300.089.6779865
GACGGCG565200.089.197394
TATCTCG171650.088.638742
CGGCGCG579150.086.845026
ATATCTC178800.085.290621
ATCTCGA183250.083.057353
TCTCGAG192350.078.061484
CTCGAGG207500.072.3657765
CGAGGGC225400.066.414187
AGGGCGC225550.066.37059
CGCGCCG789750.065.615999
GGCGCGC801300.064.112947
TCGAGGG233800.064.1092456
GAGGGCG234200.063.9191558
GCGCGCC814100.063.583438
ATCCATA585350.043.12548416-17
TCCATAT590850.042.80049518-19
Produced by FastQC (version 0.11.5)