##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048265_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6806677 Sequences flagged as poor quality 0 Sequence length 101 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.051780038923546 31.0 31.0 34.0 26.0 34.0 2 30.358121297661107 31.0 31.0 34.0 26.0 34.0 3 30.59957274305803 31.0 31.0 34.0 27.0 34.0 4 34.09213071224035 37.0 35.0 37.0 30.0 37.0 5 34.00134794114661 37.0 35.0 37.0 30.0 37.0 6 34.03836571061033 37.0 35.0 37.0 30.0 37.0 7 33.864504809027956 37.0 35.0 37.0 29.0 37.0 8 33.91547828698203 37.0 35.0 37.0 29.0 37.0 9 35.356594708401765 39.0 35.0 39.0 29.0 39.0 10-11 35.314227779575845 39.0 35.0 39.0 29.0 39.0 12-13 35.280668746291326 39.0 35.0 39.0 29.0 39.0 14-15 36.459973860960346 40.0 36.5 41.0 28.5 41.0 16-17 36.34395094698925 40.0 36.5 41.0 28.0 41.0 18-19 36.31766903292164 40.0 36.5 41.0 27.0 41.0 20-21 36.412028512591384 40.0 37.0 41.0 28.5 41.0 22-23 36.40933887416723 40.0 36.5 41.0 29.0 41.0 24-25 36.4188383700299 40.0 36.0 41.0 29.0 41.0 26-27 36.40389260427665 40.0 36.0 41.0 29.0 41.0 28-29 36.21292151809172 39.5 36.0 41.0 29.0 41.0 30-31 35.79301845819921 39.0 35.5 41.0 28.5 41.0 32-33 35.53620018990177 39.0 35.0 41.0 28.0 41.0 34-35 35.05722094643245 38.0 35.0 40.5 26.0 41.0 36-37 34.980405196250686 38.0 35.0 40.0 25.5 41.0 38-39 34.64548523457188 38.0 34.5 40.5 24.0 41.0 40-41 33.650766299032554 38.0 33.0 40.0 15.5 41.0 42-43 33.18137073347244 38.0 32.5 40.0 12.5 41.0 44-45 32.847129957834056 38.0 32.0 40.0 9.0 41.0 46-47 32.41882382548783 38.0 31.5 40.0 6.0 41.0 48-49 31.92194539273716 37.0 31.0 40.0 2.0 41.0 50-51 31.87003437947768 37.0 31.0 40.0 2.0 41.0 52-53 31.77868466507225 37.0 31.0 40.0 2.0 41.0 54-55 31.72207032888442 37.0 31.0 40.0 2.0 41.0 56-57 31.497019764563532 36.0 31.0 40.0 2.0 41.0 58-59 31.228677958422296 36.0 30.0 40.0 2.0 41.0 60-61 30.94405214174259 35.0 30.0 39.0 2.0 41.0 62-63 30.640044327062974 35.0 30.0 39.0 2.0 41.0 64-65 30.358352173902183 35.0 29.0 39.0 2.0 40.5 66-67 30.056511349076793 35.0 29.0 38.0 2.0 40.0 68-69 29.71736759361433 35.0 29.0 37.0 2.0 40.0 70-71 29.396748442742325 35.0 29.0 37.0 2.0 39.0 72-73 29.090031156172095 34.0 29.0 36.0 2.0 39.0 74-75 28.79082877004447 34.0 28.5 36.0 2.0 38.5 76-77 28.534734423272916 34.0 28.0 35.0 2.0 37.0 78-79 28.301952039152145 34.0 28.0 35.0 2.0 37.0 80-81 28.086736743935404 34.0 27.5 35.0 2.0 36.5 82-83 27.891242745909643 34.0 27.0 35.0 2.0 36.0 84-85 27.804809747840245 34.0 27.0 35.0 2.0 36.0 86-87 27.961685856402468 34.0 29.0 35.0 2.0 35.5 88-89 27.89913279269752 34.0 29.0 35.0 2.0 35.0 90-91 27.776450461803904 34.0 29.0 35.0 2.0 35.0 92-93 27.657693908496025 34.0 28.5 35.0 2.0 35.0 94-95 27.533402716185886 34.0 28.0 35.0 2.0 35.0 96-97 27.345734857111626 34.0 27.0 35.0 2.0 35.0 98-99 27.156333993812254 34.0 27.0 35.0 2.0 35.0 100-101 26.51347103733584 33.5 25.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7220315248221958 1101 2 0.7316062633097786 1101 3 0.7274892783104434 1101 4 0.7814064319364675 1101 5 0.7630025284476076 1101 6 0.681230396726832 1101 7 0.7693668027252372 1101 8 0.7698533087553159 1101 9 0.8485427494799538 1101 10-11 0.8355659699616496 1101 12-13 0.8147383138704924 1101 14-15 0.9543893398275571 1101 16-17 0.9595280517270126 1101 18-19 0.964596380643556 1101 20-21 0.9244853896468612 1101 22-23 0.9583415892640943 1101 24-25 0.9539634861959385 1101 26-27 0.9609461937871302 1101 28-29 0.9289552572640005 1101 30-31 0.8951362855881158 1101 32-33 0.8245186342254982 1101 34-35 0.9217473563900711 1101 36-37 0.9048340800866512 1101 38-39 0.8458972811975229 1101 40-41 0.5753211083116199 1101 42-43 0.5455430920072075 1101 44-45 0.5581039481037067 1101 46-47 0.4796204800995483 1101 48-49 0.38015208435937 1101 50-51 0.30574979961584603 1101 52-53 0.21747019266937428 1101 54-55 0.16929385939828023 1101 56-57 0.16607865485580575 1101 58-59 0.15578935120284498 1101 60-61 0.10829658854416735 1101 62-63 0.12055704429162617 1101 64-65 0.10878771361113593 1101 66-67 0.06511324241424177 1101 68-69 0.014209858743388537 1101 70-71 0.027078921881170004 1101 72-73 0.0479361725804921 1101 74-75 0.0013367962043311366 1101 76-77 0.06712351330077126 1101 78-79 -0.08234228883351591 1101 80-81 -0.091824565060449 1101 82-83 -0.07615827145128051 1101 84-85 -0.0741234794195762 1101 86-87 -0.03171308498932035 1101 88-89 -0.028250833574333 1101 90-91 -0.03467566191212512 1101 92-93 -0.01980686250395891 1101 94-95 -0.08892834391384596 1101 96-97 -0.02369537711049574 1101 98-99 0.019308468574120496 1101 100-101 -0.06826313043887211 1102 1 0.6515342484683551 1102 2 0.6427338547312793 1102 3 0.6262473306458389 1102 4 0.71545493485457 1102 5 0.7525853117261363 1102 6 0.6945373775318444 1102 7 0.7120896410930797 1102 8 0.7404027838681628 1102 9 0.8525795721730987 1102 10-11 0.7801236098710334 1102 12-13 0.7590936215101465 1102 14-15 0.892453064285263 1102 16-17 0.8623805939245841 1102 18-19 0.9613202899275848 1102 20-21 0.931857338151552 1102 22-23 0.9057302548201989 1102 24-25 0.8978096284969155 1102 26-27 0.9709806987182574 1102 28-29 0.9015773553671167 1102 30-31 0.9061517641660686 1102 32-33 0.8200443057681142 1102 34-35 0.8787884248855278 1102 36-37 0.8493341077638163 1102 38-39 0.7877206227008315 1102 40-41 0.5292146602002816 1102 42-43 0.561763256457688 1102 44-45 0.5035882676110077 1102 46-47 0.4189730715534665 1102 48-49 0.3836449996754361 1102 50-51 0.30081382616008767 1102 52-53 0.2769087826445187 1102 54-55 0.23116717186309899 1102 56-57 0.20644572262051142 1102 58-59 0.2591831187624365 1102 60-61 0.2245992153433214 1102 62-63 0.26708393699161803 1102 64-65 0.2654593913938257 1102 66-67 0.19247359419792787 1102 68-69 0.24042952678486174 1102 70-71 0.23902950626488817 1102 72-73 0.19734280339627475 1102 74-75 0.15481668929906078 1102 76-77 0.17109733665814275 1102 78-79 0.1700646576052236 1102 80-81 0.12961047494200528 1102 82-83 0.12020553144080992 1102 84-85 0.09430651050567107 1102 86-87 0.14298165698006216 1102 88-89 0.18573133600289538 1102 90-91 0.1506161049565371 1102 92-93 0.1703246175426969 1102 94-95 0.12676446452156753 1102 96-97 0.14054039616213032 1102 98-99 0.15733866399819263 1102 100-101 0.19902613179471018 1103 1 0.5344596315729859 1103 2 0.5060141656729513 1103 3 0.5079107010650006 1103 4 0.5658474783824801 1103 5 0.6248782685287253 1103 6 0.5323531235743388 1103 7 0.613192727766652 1103 8 0.6147938415044365 1103 9 0.6691256266381558 1103 10-11 0.6161074460554019 1103 12-13 0.5995963102978195 1103 14-15 0.7123748192457455 1103 16-17 0.7389152798595546 1103 18-19 0.8035274939022585 1103 20-21 0.8181162309642929 1103 22-23 0.7520599794496192 1103 24-25 0.8003670744112839 1103 26-27 0.8012200515246448 1103 28-29 0.7952169001995841 1103 30-31 0.7403190613488562 1103 32-33 0.6902059899948227 1103 34-35 0.7804167984577717 1103 36-37 0.804309258159698 1103 38-39 0.7473027920054207 1103 40-41 0.5462654680816428 1103 42-43 0.538667722188066 1103 44-45 0.46327255085009966 1103 46-47 0.4393142864358168 1103 48-49 0.33593497845363984 1103 50-51 0.2865760800615149 1103 52-53 0.3052022862194761 1103 54-55 0.19899497285301493 1103 56-57 0.1847489995511289 1103 58-59 0.20114735567094044 1103 60-61 0.19185613344378183 1103 62-63 0.18076325713083108 1103 64-65 0.2612544184283472 1103 66-67 0.21377055375921472 1103 68-69 0.1804635457163819 1103 70-71 0.10681483287624616 1103 72-73 0.20312215584696958 1103 74-75 0.14910102364149935 1103 76-77 0.1590716497429412 1103 78-79 0.13717581085792574 1103 80-81 0.1352300727461504 1103 82-83 0.1739321255032671 1103 84-85 0.12714688845121103 1103 86-87 0.131126451629477 1103 88-89 0.10577221404737003 1103 90-91 0.124662113219415 1103 92-93 0.09511597582364573 1103 94-95 0.09764221559784048 1103 96-97 0.15795935081716195 1103 98-99 0.1868920981295581 1103 100-101 0.1647953713182133 1104 1 0.4986212960301408 1104 2 0.42559544110049785 1104 3 0.5171136005518058 1104 4 0.5218463206886099 1104 5 0.5504220153698753 1104 6 0.46094954903721685 1104 7 0.484213710194183 1104 8 0.5250172229896037 1104 9 0.5613185563154985 1104 10-11 0.5091996005166735 1104 12-13 0.5334202176392537 1104 14-15 0.6025366711084175 1104 16-17 0.6317123662308433 1104 18-19 0.6820368975955375 1104 20-21 0.6942094529774465 1104 22-23 0.6859947763108352 1104 24-25 0.6578430213647408 1104 26-27 0.6772673983350828 1104 28-29 0.6963040302184709 1104 30-31 0.6068291103094765 1104 32-33 0.5822786882009723 1104 34-35 0.575163048852886 1104 36-37 0.6064829871174027 1104 38-39 0.6734339741049951 1104 40-41 0.5098364197576188 1104 42-43 0.42828537882625994 1104 44-45 0.3821581518419066 1104 46-47 0.3870242231230776 1104 48-49 0.2991348316044373 1104 50-51 0.2171048833715048 1104 52-53 0.1947956902008876 1104 54-55 0.15876581898263709 1104 56-57 0.13999298486948675 1104 58-59 0.1742252021268662 1104 60-61 0.17816583492427895 1104 62-63 0.165449785700023 1104 64-65 0.1998995313905425 1104 66-67 0.14607917837117412 1104 68-69 0.12779767496846262 1104 70-71 0.11862165843674433 1104 72-73 0.1314351868657191 1104 74-75 0.1329294127929863 1104 76-77 0.08561942622750252 1104 78-79 0.1256903646117209 1104 80-81 0.10435936918775468 1104 82-83 0.1540524478144505 1104 84-85 0.10531749947319824 1104 86-87 0.15931245942154604 1104 88-89 0.14809363121502628 1104 90-91 0.12633198577202975 1104 92-93 0.07050645851868609 1104 94-95 0.07702362849723343 1104 96-97 0.06471467319293467 1104 98-99 0.06486556615801575 1104 100-101 0.10169690792911723 1105 1 0.2044733843167812 1105 2 0.19168899754764723 1105 3 0.1698235852203176 1105 4 0.19624589849241403 1105 5 0.27494084317680745 1105 6 0.1983265480631431 1105 7 0.19483567048109052 1105 8 0.17418961837830693 1105 9 0.25998042986047665 1105 10-11 0.16657182759104217 1105 12-13 0.1579943942891191 1105 14-15 0.18320906094340472 1105 16-17 0.16367253595809927 1105 18-19 0.24062828841658757 1105 20-21 0.16975892248294855 1105 22-23 0.14162656173436972 1105 24-25 0.12228748801745581 1105 26-27 0.1349212216042801 1105 28-29 0.09155047833591112 1105 30-31 0.13633891671527465 1105 32-33 0.06312698227461055 1105 34-35 0.10241663027802872 1105 36-37 0.12443865480235416 1105 38-39 0.15141261709376863 1105 40-41 0.11603919888078451 1105 42-43 0.013193814430870532 1105 44-45 -0.010948418290162465 1105 46-47 0.059455131906247516 1105 48-49 -0.030324141857892783 1105 50-51 -0.1337141812422189 1105 52-53 -0.16199309389751448 1105 54-55 -0.2242177051099965 1105 56-57 -0.2868000763238392 1105 58-59 -0.31357988830106365 1105 60-61 -0.25952231174338536 1105 62-63 -0.22662020711484843 1105 64-65 -0.311890780396638 1105 66-67 -0.24735119712784126 1105 68-69 -0.25606651851096274 1105 70-71 -0.2582878133213846 1105 72-73 -0.22935814648397468 1105 74-75 -0.20017710057003057 1105 76-77 -0.21797110118155416 1105 78-79 -0.20170083052166632 1105 80-81 -0.22995923248713268 1105 82-83 -0.17978076858547354 1105 84-85 -0.23473809320071126 1105 86-87 -0.1978695292278161 1105 88-89 -0.2379224790345802 1105 90-91 -0.26150970295596565 1105 92-93 -0.2838384473343254 1105 94-95 -0.17547447355852697 1105 96-97 -0.16961472938599087 1105 98-99 -0.16866014528119067 1105 100-101 -0.25145510366312607 1106 1 0.32461193123421594 1106 2 0.2948410886513315 1106 3 0.33719013738321735 1106 4 0.34232305347922676 1106 5 0.3708382164886359 1106 6 0.27862212605828773 1106 7 0.3538145878661112 1106 8 0.33224505859610787 1106 9 0.3900146807596556 1106 10-11 0.3222689912048864 1106 12-13 0.36260729728162033 1106 14-15 0.3980838648889957 1106 16-17 0.4223236647453348 1106 18-19 0.47878396171377346 1106 20-21 0.4734517946781338 1106 22-23 0.48641788379963913 1106 24-25 0.4500888376618448 1106 26-27 0.396011338112082 1106 28-29 0.42966398651703486 1106 30-31 0.3896108088000645 1106 32-33 0.3305751909092436 1106 34-35 0.308007591407474 1106 36-37 0.3143509583087294 1106 38-39 0.2716830818631806 1106 40-41 0.26503301642918586 1106 42-43 0.2167391590248613 1106 44-45 0.17693654459714736 1106 46-47 0.1799374568313752 1106 48-49 0.06306485599677458 1106 50-51 0.12213310115701148 1106 52-53 0.04288743666007022 1106 54-55 0.021364487186357195 1106 56-57 0.04252535945971658 1106 58-59 0.07343323317097372 1106 60-61 0.02629708913420359 1106 62-63 0.051345618183869846 1106 64-65 0.07050732811863014 1106 66-67 0.13537878703252915 1106 68-69 0.06501612304737492 1106 70-71 0.04832696075288467 1106 72-73 0.12828481327709795 1106 74-75 0.05476316769858869 1106 76-77 0.10558938636882687 1106 78-79 0.02462519470409319 1106 80-81 -0.018326303703148028 1106 82-83 0.010471269542257744 1106 84-85 -0.0150938682963222 1106 86-87 0.013006356143282716 1106 88-89 0.020735146696182483 1106 90-91 0.027100422287073656 1106 92-93 -0.008078877754126523 1106 94-95 0.014814443859332727 1106 96-97 0.05733837665322383 1106 98-99 0.04332109370995241 1106 100-101 0.0556238578061965 1107 1 0.16079780972286173 1107 2 0.18621944043177763 1107 3 0.18558792983582606 1107 4 0.2091923452687965 1107 5 0.23753203593696526 1107 6 0.14833911495217222 1107 7 0.1667615526030275 1107 8 0.23230394116310293 1107 9 0.2581219257585232 1107 10-11 0.1419120969104597 1107 12-13 0.19174968457625852 1107 14-15 0.13932269444357814 1107 16-17 0.17042372156916485 1107 18-19 0.2576227070120609 1107 20-21 0.23739604292948968 1107 22-23 0.25625674604252424 1107 24-25 0.2716841678762165 1107 26-27 0.2472127147170866 1107 28-29 0.2821394919087936 1107 30-31 0.2874926146227921 1107 32-33 0.18317115219569757 1107 34-35 0.21591859789759837 1107 36-37 0.2160660539863315 1107 38-39 0.23886499532373762 1107 40-41 0.08151791568052147 1107 42-43 0.09521302164807821 1107 44-45 0.137632865418027 1107 46-47 0.16511167028099294 1107 48-49 0.2728116672420562 1107 50-51 0.23294854173249036 1107 52-53 0.1303760887094505 1107 54-55 0.1571083842047365 1107 56-57 0.08260264158147024 1107 58-59 0.04331228310772417 1107 60-61 0.1260284159386984 1107 62-63 0.11566414090006205 1107 64-65 0.17379540258587411 1107 66-67 0.14532675666507444 1107 68-69 0.1361256748560855 1107 70-71 0.09738218957732059 1107 72-73 0.15016526248333406 1107 74-75 0.1155106480857917 1107 76-77 0.06489851470798413 1107 78-79 0.10190144889611474 1107 80-81 0.15328333596185928 1107 82-83 0.18852174207753336 1107 84-85 0.1749156125694462 1107 86-87 0.20231049179074034 1107 88-89 0.18018326871892398 1107 90-91 0.18178507548165257 1107 92-93 0.08651475935081265 1107 94-95 0.18126382913509076 1107 96-97 0.11989312051239054 1107 98-99 0.07522408136357583 1107 100-101 0.029526694585019442 1108 1 0.08046473309598667 1108 2 0.06230152945186873 1108 3 0.12088898946303317 1108 4 0.1308077419515783 1108 5 0.18143989258066995 1108 6 0.06813795692082891 1108 7 0.09912118408664838 1108 8 0.0872754895308816 1108 9 0.10475051430085358 1108 10-11 0.10106992146585725 1108 12-13 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-1.2270143211755737 2215 34-35 -1.0514484693710884 2215 36-37 -1.0696830755064326 2215 38-39 -1.137351099770271 2215 40-41 -0.9627336953134602 2215 42-43 -0.9211662809651742 2215 44-45 -0.8224592266269113 2215 46-47 -0.9553144195642282 2215 48-49 -0.8136262323813845 2215 50-51 -0.7269639272464872 2215 52-53 -0.732239114226136 2215 54-55 -0.7091312205225151 2215 56-57 -0.7060601341131054 2215 58-59 -0.7517621502663232 2215 60-61 -0.7242510915305118 2215 62-63 -0.7757353493401844 2215 64-65 -0.7132962221705235 2215 66-67 -0.7659816760434559 2215 68-69 -0.725742314850816 2215 70-71 -0.6732232178091557 2215 72-73 -0.8049382353244567 2215 74-75 -0.7138754826432354 2215 76-77 -0.6845411554475405 2215 78-79 -0.6218218783454468 2215 80-81 -0.704141679816189 2215 82-83 -0.5970069496982049 2215 84-85 -0.6320406272913637 2215 86-87 -0.8127870730886642 2215 88-89 -1.0172913593433464 2215 90-91 -0.8924827279931655 2215 92-93 -0.8310198356549989 2215 94-95 -0.8252740217456598 2215 96-97 -0.9609841186099999 2215 98-99 -0.9860746665403362 2215 100-101 -0.965114352816741 2216 1 -0.9200910906947826 2216 2 -0.9884755604472275 2216 3 -0.9942575248171082 2216 4 -1.0114133448730698 2216 5 -1.2431782305473078 2216 6 -0.9496086210914712 2216 7 -1.16831182827827 2216 8 -1.0299299847553272 2216 9 -1.2339336184227179 2216 10-11 -1.1341206062349798 2216 12-13 -1.221721883185353 2216 14-15 -1.3217722986451435 2216 16-17 -1.4772724563257924 2216 18-19 -1.1951236088556385 2216 20-21 -1.3686163565818035 2216 22-23 -1.4868922636487412 2216 24-25 -1.4484880119450878 2216 26-27 -1.849593347998571 2216 28-29 -1.6058982716383028 2216 30-31 -1.4189325752670214 2216 32-33 -1.4758248168185375 2216 34-35 -1.3235267761597314 2216 36-37 -1.5981253705800995 2216 38-39 -1.4556472033516599 2216 40-41 -1.3265360855491082 2216 42-43 -1.1485454579459287 2216 44-45 -0.9160985595215081 2216 46-47 -1.617574699761665 2216 48-49 -1.341134834815417 2216 50-51 -1.1510605135168603 2216 52-53 -1.2938623089854282 2216 54-55 -1.1332935476715527 2216 56-57 -1.1504867550296396 2216 58-59 -1.116173484035361 2216 60-61 -1.081369361968509 2216 62-63 -0.9629506860111405 2216 64-65 -0.8955695686180434 2216 66-67 -1.2218892820805642 2216 68-69 -1.0774898063250014 2216 70-71 -1.2784640533477507 2216 72-73 -1.1345093671072988 2216 74-75 -0.8591052835987192 2216 76-77 -1.042384519800791 2216 78-79 -1.0531346298934494 2216 80-81 -1.034410878955967 2216 82-83 -1.1243255907503098 2216 84-85 -1.0021039512892536 2216 86-87 -0.9348225807768848 2216 88-89 -0.8865928861427896 2216 90-91 -0.8743766221556335 2216 92-93 -0.9571370660454335 2216 94-95 -1.000813503054058 2216 96-97 -1.0441702918880686 2216 98-99 -1.041393719921846 2216 100-101 -1.1566401509690039 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 187301.0 3 29958.0 4 17403.0 5 13907.0 6 13572.0 7 17193.0 8 23057.0 9 25069.0 10 27919.0 11 28291.0 12 32298.0 13 44784.0 14 61429.0 15 99813.0 16 67855.0 17 76426.0 18 107892.0 19 48492.0 20 38887.0 21 36635.0 22 38926.0 23 44811.0 24 55289.0 25 69104.0 26 85592.0 27 104516.0 28 124851.0 29 145565.0 30 165858.0 31 194379.0 32 234347.0 33 289124.0 34 377181.0 35 537222.0 36 880891.0 37 1315242.0 38 1003247.0 39 142316.0 40 35.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.327425346680656 30.63496381948974 13.282645761846695 22.75496507198291 2 24.565522435039433 25.503137346037647 31.10152763600314 18.82981258291978 3 22.771784298295056 26.28861770496839 19.95495437839823 30.984643618338325 4 31.349249196150463 22.96741323882591 22.85780608580809 22.825531479215535 5 23.467612164750975 31.28705058819774 21.204965777278478 24.040371469772808 6 22.920817093863704 21.933099391805968 23.761020712724857 31.385062801605475 7 31.67686485651555 22.338529886617312 20.91690295721762 25.06770229964952 8 32.750931555495406 21.670518420011266 22.366511370640666 23.21203865385266 9 21.948082020603717 23.404783054261518 23.099946538492432 31.547188386642333 10-11 29.264396414480544 22.355987665497583 21.552308591810974 26.827307328210903 12-13 28.079322273256395 22.319074590154106 21.711542087595593 27.890061048993907 14-15 27.825038697951044 22.33207391667253 21.811108980330047 28.031778405046374 16-17 27.94152345631286 25.96605757952069 21.979843607909455 24.112575356256993 18-19 23.574414461998742 21.796641751929847 26.25676830324647 28.37217548282494 20-21 23.67069744911345 27.315014484359878 22.286240733636838 26.728047332889833 22-23 22.747181388432384 26.044034144196456 27.509600352016584 23.699184115354576 24-25 22.513426383689996 21.88562339619088 31.811075699790475 23.78987452032865 26-27 22.429297215540334 21.94908423757451 33.088828804419215 22.53278974246594 28-29 22.580265717603414 21.998461793669225 32.96622603541111 22.455046453316246 30-31 22.555010079561466 21.834691365718953 32.98960963028756 22.620688924432024 32-33 22.504940308013914 21.889025770229736 33.046757949993236 22.55927597176311 34-35 22.64938024590392 21.91711426623241 32.82225748641304 22.611248001450633 36-37 22.654285261156755 21.803292894025788 32.74147286331306 22.800948981504394 38-39 23.055511315925273 21.913844413245798 31.97290401316179 23.057740257667135 40-41 25.157206887198804 22.744917328587498 27.60350561482332 24.494370169390383 42-43 25.37573643811622 22.951814975018657 27.298941108075436 24.373507478789683 44-45 25.508861760711348 23.57409464207132 26.725127622801775 24.191915974415558 46-47 25.035153029371358 24.36528066938079 26.194754111292855 24.404812189954992 48-49 24.902125290972148 24.840068913224425 25.313948622122258 24.943857173681174 50-51 24.98068963295334 25.145168870180417 24.978056317496637 24.896085179369607 52-53 25.169797149868238 25.145465787765186 24.739741980141723 24.944995082224846 54-55 25.26777410945673 24.660332815634515 24.9106413966065 25.16125167830226 56-57 25.147555521212222 24.62525228551821 25.263670333430845 24.96352185983872 58-59 25.13060679423928 24.604286783106808 25.00899436024729 25.256112062406622 60-61 25.17418134876464 24.637791542834872 24.80238057287226 25.385646535528227 62-63 25.201583444832387 24.731914610457746 24.73561713723721 25.330884807472653 64-65 25.230030508868147 24.81440181031351 24.681509518699173 25.27405816211918 66-67 25.361654120181104 24.697064874298974 24.62441842003306 25.316862585486867 68-69 25.32146803065704 24.979607170124137 24.328015146205573 25.37090965301325 70-71 25.29084513110779 24.978002319976117 24.136942907879522 25.59420964103657 72-73 25.390369497295044 24.846074683656834 24.188035861154596 25.575519957893523 74-75 25.364460770985335 24.896293834173303 24.278578680442106 25.460666714399256 76-77 25.34861966721379 24.866173327936437 24.154846975396907 25.63036002945287 78-79 25.554700793383628 24.655692109024486 24.32366667218522 25.465940425406668 80-81 25.57676245480225 24.71423072146295 24.32467510299172 25.38433172074308 82-83 25.780820637683462 24.89101589848925 24.26425935879932 25.063904105027966 84-85 25.942425910944518 24.792600967793483 24.09303571854237 25.171937402719628 86-87 25.723665420810754 24.879508649847978 24.14784235324481 25.248983576096457 88-89 25.7108914608862 24.721648835310546 24.29636242100736 25.271097282795896 90-91 25.679165474289807 24.714405352307168 24.054572387841088 25.551856785561938 92-93 25.566704565796712 24.932359507424618 24.052593485808355 25.448342440970322 94-95 25.864891509085812 24.908762138710394 23.931366316545322 25.294980035658465 96-97 25.905835853034166 24.906447115495347 23.91165002085739 25.276067010613097 98-99 26.086018454307514 25.06294586037881 23.892464863935686 24.958570821377986 100-101 26.250745850108753 25.069531143044394 23.55431977329835 25.1254032335485 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 623.0 1 837.5 2 1162.0 3 1204.5 4 1080.0 5 973.0 6 877.5 7 811.0 8 743.0 9 683.5 10 631.5 11 530.0 12 459.5 13 453.5 14 458.5 15 478.5 16 590.5 17 684.5 18 768.0 19 962.0 20 1287.5 21 1754.0 22 2452.5 23 3472.5 24 4908.0 25 6770.5 26 9187.5 27 12739.0 28 17219.0 29 22474.5 30 29120.0 31 36656.0 32 44909.0 33 54315.5 34 64214.5 35 74845.0 36 87053.5 37 98733.5 38 110929.0 39 123883.5 40 135502.5 41 146258.0 42 159633.0 43 177590.5 44 199044.0 45 220061.0 46 237659.5 47 256145.0 48 275231.5 49 286450.0 50 289736.5 51 291669.0 52 296644.5 53 302883.5 54 300684.0 55 294903.5 56 280713.0 57 257955.5 58 248058.0 59 238790.5 60 206066.0 61 171300.0 62 144196.5 63 112950.0 64 89006.0 65 72866.0 66 55830.5 67 40141.5 68 30487.5 69 24914.0 70 22310.0 71 21688.5 72 21386.0 73 18279.5 74 13906.0 75 11987.0 76 10027.5 77 7400.0 78 5230.5 79 3385.5 80 1882.5 81 1438.0 82 1179.0 83 635.5 84 262.5 85 144.0 86 101.5 87 78.0 88 57.0 89 40.5 90 32.5 91 25.0 92 25.0 93 23.5 94 16.0 95 14.5 96 15.5 97 10.0 98 10.5 99 14.5 100 41.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5315515926493941 2 0.11660609134236867 3 0.14056785712029526 4 0.12874123452603967 5 0.12793320441090417 6 0.12755122653829468 7 0.1307833469988366 8 0.13085680428203073 9 0.13608696284545307 10-11 0.15600123231938287 12-13 0.15059477627629458 14-15 0.13931173757767557 16-17 0.12686072807626983 18-19 0.14440967303134847 20-21 0.15758056390805675 22-23 0.13618980304192485 24-25 0.15215941640832964 26-27 0.1760550706313815 28-29 0.14396892933218366 30-31 0.16881218250843988 32-33 0.14744345882726623 34-35 0.425295632509079 36-37 0.1394953807856609 38-39 0.1429405273674658 40-41 0.14662808298381133 42-43 0.2557782600819754 44-45 0.1452985061579975 46-47 0.177751933873166 48-49 0.28355245885767755 50-51 0.13461047145325097 52-53 0.16044539795262797 54-55 0.17429944156304172 56-57 0.18422352052256924 58-59 0.17837632078031615 60-61 0.17891255894763333 62-63 0.20595218489139414 64-65 0.1608420672818763 66-67 0.1579331588673886 68-69 0.15842532266478931 70-71 0.1793900312883952 72-73 0.18324653865608725 74-75 0.1745124676843047 76-77 0.16768094034725023 78-79 0.16769563180388905 80-81 0.1613856511775129 82-83 0.16945860660054826 84-85 0.15571474891492573 86-87 0.15493610171306793 88-89 0.15155706668613775 90-91 0.17299924765050553 92-93 0.1617015174952477 94-95 0.17537926362599549 96-97 0.17223529190528652 98-99 0.17554821537734197 100-101 0.14548214936598286 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6806677.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.9130748974812 #Duplication Level Percentage of deduplicated Percentage of total 1 87.97534914155385 56.2277507881619 2 6.78569942096638 8.67389830648038 3 1.868788351914332 3.5832002971032346 4 0.8487265576981363 2.1697889619856956 5 0.5320749422827762 1.7003272818596027 6 0.3474499500827318 1.3323956809658244 7 0.264806792801661 1.1847231467186041 8 0.201038868717087 1.027920981889606 9 0.16459133713253676 0.9467584611865559 >10 0.9039475284264661 9.928869269597204 >50 0.04160872582252382 1.8743443172105463 >100 0.06246922981749456 8.45560942161376 >500 0.002731580258947445 1.1218068993042358 >1k 6.018350213661651E-4 0.8451084225428735 >5k 9.259000292368521E-5 0.44302949327959357 >10k+ 2.3147500730921303E-5 0.4844682701004091 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 32747 0.4811011305516627 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA 7953 0.1168411546485899 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC 7947 0.11675300590875694 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC 7329 0.10767368570596195 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.4074369916480536E-5 0.0 0.0 0.0 0.0 2 4.4074369916480536E-5 0.0 0.0 1.4691456638826846E-5 2.9382913277653692E-5 3 4.4074369916480536E-5 0.0 0.0 1.4691456638826846E-5 2.9382913277653692E-5 4 4.4074369916480536E-5 1.4691456638826846E-5 0.0 1.4691456638826846E-5 2.9382913277653692E-5 5 7.345728319413422E-5 1.4691456638826846E-5 0.0 1.4691456638826846E-5 2.9382913277653692E-5 6 1.1753165311061477E-4 1.4691456638826846E-5 0.0 1.4691456638826846E-5 5.8765826555307384E-5 7 1.4691456638826845E-4 1.4691456638826846E-5 0.0 1.4691456638826846E-5 7.345728319413422E-5 8 1.90988936304749E-4 1.4691456638826846E-5 0.0 1.4691456638826846E-5 7.345728319413422E-5 9 3.3790350269301744E-4 1.4691456638826846E-5 0.0 1.4691456638826846E-5 7.345728319413422E-5 10-11 5.068552540395261E-4 1.4691456638826846E-5 0.0 4.4074369916480536E-5 7.345728319413422E-5 12-13 6.684612770666215E-4 1.4691456638826846E-5 0.0 4.4074369916480536E-5 8.814873983296107E-5 14-15 8.521044850519572E-4 1.4691456638826846E-5 0.0 4.4074369916480536E-5 1.1018592479120134E-4 16-17 0.0011238964328702536 1.4691456638826846E-5 0.0 7.345728319413422E-5 1.3956883806885503E-4 18-19 0.0016821717851456738 1.4691456638826846E-5 0.0 7.345728319413422E-5 1.5426029470768187E-4 20-21 0.0025930420967529384 2.9382913277653692E-5 0.0 9.54944681523745E-5 1.616060230270953E-4 22-23 0.004054842032316209 4.4074369916480536E-5 0.0 1.0284019647178792E-4 1.90988936304749E-4 24-25 0.005692939447545403 4.4074369916480536E-5 0.0 1.0284019647178792E-4 2.0568039294357584E-4 26-27 0.007037207729998059 4.4074369916480536E-5 0.0 1.0284019647178792E-4 2.3506330622122956E-4 28-29 0.00828598154429834 4.4074369916480536E-5 0.0 1.0284019647178792E-4 2.497547628600564E-4 30-31 0.010048956340957562 5.8765826555307384E-5 0.0 1.0284019647178792E-4 2.5710049117946983E-4 32-33 0.012406935131489272 5.8765826555307384E-5 0.0 1.0284019647178792E-4 2.7913767613771005E-4 34-35 0.01575658724514179 6.61115548747208E-5 0.0 1.1018592479120134E-4 3.1586631773477717E-4 36-37 0.019928960930568618 7.345728319413422E-5 0.0 1.1753165311061477E-4 3.232120460541906E-4 38-39 0.02529868833205983 8.080301151354765E-5 0.0 1.322231097494416E-4 3.4524923101243087E-4 40-41 0.03333491511349811 8.814873983296107E-5 0.0 1.322231097494416E-4 3.599406876512577E-4 42-43 0.04305331368008207 8.814873983296107E-5 1.4691456638826846E-5 1.322231097494416E-4 3.81977872609498E-4 44-45 0.05512969103719774 8.814873983296107E-5 1.4691456638826846E-5 1.322231097494416E-4 4.0401505756773826E-4 46-47 0.07179714859394679 8.814873983296107E-5 1.4691456638826846E-5 1.4691456638826845E-4 4.3339797084539195E-4 48-49 0.09341562703798051 8.814873983296107E-5 1.4691456638826846E-5 1.5426029470768187E-4 4.554351558036322E-4 50-51 0.11887592139306742 8.814873983296107E-5 1.4691456638826846E-5 1.7629747966592214E-4 4.7012661244245907E-4 52-53 0.14898606177434304 8.814873983296107E-5 2.9382913277653692E-5 1.8364320798533557E-4 4.7012661244245907E-4 54-55 0.18774212438756827 8.814873983296107E-5 2.9382913277653692E-5 1.90988936304749E-4 4.995095257201128E-4 56-57 0.23370434648213806 8.814873983296107E-5 2.9382913277653692E-5 1.90988936304749E-4 5.21546710678353E-4 58-59 0.2874310034103278 8.814873983296107E-5 2.9382913277653692E-5 1.90988936304749E-4 5.288924389977664E-4 60-61 0.3515004458122517 9.54944681523745E-5 2.9382913277653692E-5 1.90988936304749E-4 5.362381673171798E-4 62-63 0.42398074713990397 1.4691456638826845E-4 2.9382913277653692E-5 1.90988936304749E-4 6.023497221919006E-4 64-65 0.5078395816343276 1.4691456638826845E-4 2.9382913277653692E-5 1.90988936304749E-4 6.170411788307275E-4 66-67 0.6028565774459402 1.616060230270953E-4 2.9382913277653692E-5 1.90988936304749E-4 6.317326354695543E-4 68-69 0.7108755123829146 1.616060230270953E-4 2.9382913277653692E-5 1.90988936304749E-4 6.390783637889678E-4 70-71 0.829839582515815 1.616060230270953E-4 2.9382913277653692E-5 1.90988936304749E-4 6.464240921083812E-4 72-73 0.9648026489283978 1.6895175134650872E-4 2.9382913277653692E-5 2.203718495824027E-4 6.464240921083812E-4 74-75 1.109719177213786 1.7629747966592214E-4 2.9382913277653692E-5 2.277175779018161E-4 6.684612770666215E-4 76-77 1.2732644725172062 1.7629747966592214E-4 2.9382913277653692E-5 2.4240903454064299E-4 6.904984620248617E-4 78-79 1.45405019218629 1.7629747966592214E-4 2.9382913277653692E-5 2.5710049117946983E-4 6.978441903442752E-4 80-81 1.6506879935686678 1.7629747966592214E-4 2.9382913277653692E-5 2.644462194988832E-4 7.63955745218996E-4 82-83 1.8639491781378785 1.8364320798533557E-4 2.9382913277653692E-5 2.644462194988832E-4 7.933386584966497E-4 84-85 2.0939365860903933 1.90988936304749E-4 2.9382913277653692E-5 2.644462194988832E-4 8.153758434548899E-4 86-87 2.3428318987370784 1.90988936304749E-4 2.9382913277653692E-5 2.644462194988832E-4 8.447587567325436E-4 88-89 2.610796722100961 1.90988936304749E-4 2.9382913277653692E-5 2.644462194988832E-4 8.741416700101973E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 53605 0.0 94.59815 1 TCGACGG 54195 0.0 93.218765 2 CGACGGC 56300 0.0 89.80484 3 ACGGCGC 56130 0.0 89.677986 5 GACGGCG 56520 0.0 89.19739 4 TATCTCG 17165 0.0 88.63874 2 CGGCGCG 57915 0.0 86.84502 6 ATATCTC 17880 0.0 85.29062 1 ATCTCGA 18325 0.0 83.05735 3 TCTCGAG 19235 0.0 78.06148 4 CTCGAGG 20750 0.0 72.365776 5 CGAGGGC 22540 0.0 66.41418 7 AGGGCGC 22555 0.0 66.3705 9 CGCGCCG 78975 0.0 65.61599 9 GGCGCGC 80130 0.0 64.11294 7 TCGAGGG 23380 0.0 64.109245 6 GAGGGCG 23420 0.0 63.919155 8 GCGCGCC 81410 0.0 63.58343 8 ATCCATA 58535 0.0 43.125484 16-17 TCCATAT 59085 0.0 42.800495 18-19 >>END_MODULE