FastQCFastQC Report
Tue 31 May 2016
SRR2048265_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048265_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6806677
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT149830.22012209481954265No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGC102490.15057273909133634TruSeq Adapter, Index 23 (97% over 38bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT101830.14960310295317378No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101080.14850124370526177No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG57000.090.087611
TCGACGG62300.082.965872
CGACGGC81250.063.726073
ACGGCGC81450.063.3363045
GACGGCG84000.061.583254
TATCTCG44950.057.8117752
ATCTCGA108750.052.158771
CGGCGCG101550.050.8449256
ATATCTC51400.050.598821
TCTCGAG133700.042.390422
CTCGAGG192350.029.8324383
CGCGCCG356300.029.0082829
GGCGCGC374800.027.539237
GCGCGCC377400.027.4871468
TCGAGGG220850.025.875114
CGAGGGC226600.025.239495
ATCCATA108250.024.74742916-17
TCCATAT107900.024.34347518-19
CCATATT111050.024.27315318-19
GGGCGCG251750.022.659618
Produced by FastQC (version 0.11.5)