##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048265_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6806677 Sequences flagged as poor quality 0 Sequence length 101 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16820469077643 33.0 31.0 34.0 30.0 34.0 2 32.30768317638695 34.0 31.0 34.0 30.0 34.0 3 32.360949109234944 34.0 31.0 34.0 30.0 34.0 4 35.9411073861739 37.0 35.0 37.0 35.0 37.0 5 35.734654957183956 37.0 35.0 37.0 33.0 37.0 6 35.71412820088275 37.0 35.0 37.0 33.0 37.0 7 35.64775175904483 37.0 35.0 37.0 33.0 37.0 8 35.62259352103824 37.0 35.0 37.0 33.0 37.0 9 37.169962817392395 39.0 37.0 39.0 33.0 39.0 10-11 37.20256066212632 39.0 37.0 39.0 33.0 39.0 12-13 37.211078548313665 39.0 37.0 39.0 33.0 39.0 14-15 38.56578364156255 40.0 38.0 41.0 33.5 41.0 16-17 38.61462428729908 40.0 38.0 41.0 34.0 41.0 18-19 38.69453905334424 40.0 38.0 41.0 34.0 41.0 20-21 38.74586688923244 40.0 38.0 41.0 34.0 41.0 22-23 38.74016234353415 40.0 38.0 41.0 34.0 41.0 24-25 38.68999777718261 40.0 38.0 41.0 34.0 41.0 26-27 38.533095958571266 40.0 38.0 41.0 34.0 41.0 28-29 38.325959274988364 40.0 38.0 41.0 34.0 41.0 30-31 38.141969936284625 40.0 37.0 41.0 33.5 41.0 32-33 38.004491839410036 40.0 37.0 41.0 33.0 41.0 34-35 37.897065557833876 40.0 37.0 41.0 33.0 41.0 36-37 37.74274017997328 40.0 36.5 41.0 33.0 41.0 38-39 37.56243949874513 40.0 36.0 41.0 33.0 41.0 40-41 37.2803724930682 40.0 36.0 41.0 32.0 41.0 42-43 36.995912102190246 40.0 35.5 41.0 31.0 41.0 44-45 36.682135644162344 39.0 35.0 41.0 31.0 41.0 46-47 36.31104296560569 39.0 35.0 41.0 30.0 41.0 48-49 36.17264650577661 39.0 35.0 41.0 30.0 41.0 50-51 36.10421730015983 39.0 35.0 41.0 30.0 41.0 52-53 35.914285561074806 39.0 35.0 41.0 29.5 41.0 54-55 35.64869010238036 38.5 35.0 41.0 29.0 41.0 56-57 35.407177393609246 38.0 35.0 40.0 28.5 41.0 58-59 35.139197438045024 37.5 34.5 40.0 28.0 41.0 60-61 34.836865771653336 37.0 34.0 40.0 28.0 41.0 62-63 34.524307382295355 36.5 34.0 40.0 28.0 41.0 64-65 34.2008403660112 36.0 34.0 39.0 27.0 41.0 66-67 33.85598589443865 35.0 34.0 39.0 26.5 41.0 68-69 33.51352708524291 35.0 33.0 38.5 26.0 40.0 70-71 33.17319626008403 35.0 33.0 37.0 26.0 39.5 72-73 32.84260307929993 35.0 33.0 37.0 26.0 39.0 74-75 32.616277149628225 35.0 33.0 36.0 26.0 39.0 76-77 32.48427683581871 35.0 33.0 36.0 26.0 38.0 78-79 32.3113519269388 35.0 33.0 35.5 26.0 37.0 80-81 32.11974411302314 35.0 33.0 35.0 26.0 37.0 82-83 31.947297484514102 35.0 33.0 35.0 26.0 36.0 84-85 31.818679144022845 35.0 33.0 35.0 26.0 36.0 86-87 31.69310355111606 35.0 33.0 35.0 25.0 36.0 88-89 31.591307623382157 35.0 33.0 35.0 25.0 35.5 90-91 31.489527782793278 35.0 33.0 35.0 25.0 35.0 92-93 31.3658805904849 35.0 33.0 35.0 25.0 35.0 94-95 31.267067983393368 35.0 33.0 35.0 24.5 35.0 96-97 31.16347117690468 35.0 33.0 35.0 24.0 35.0 98-99 31.03081973773693 35.0 33.0 35.0 23.5 35.0 100-101 30.495908723155218 34.5 32.0 35.0 20.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3831787060622105 1101 2 0.4175449171431467 1101 3 0.38487270940287743 1101 4 0.25021082501123004 1101 5 0.2750576981898618 1101 6 0.20005181762968505 1101 7 0.22656586881571172 1101 8 0.26183527521125427 1101 9 0.3343109905215371 1101 10-11 0.30377374816144 1101 12-13 0.3046387974331495 1101 14-15 0.41673091227098524 1101 16-17 0.49929995177787134 1101 18-19 0.5044470817663793 1101 20-21 0.5071778626407166 1101 22-23 0.506102442611521 1101 24-25 0.5326270283595136 1101 26-27 0.5297432054204094 1101 28-29 0.5727488996027148 1101 30-31 0.5465350356103897 1101 32-33 0.5222424500921221 1101 34-35 0.5983306526065064 1101 36-37 0.6338085676791323 1101 38-39 0.6381891878064891 1101 40-41 0.6234425546845017 1101 42-43 0.6649106584652031 1101 44-45 0.6341107405643598 1101 46-47 0.6089761359025587 1101 48-49 0.5950520241482309 1101 50-51 0.5572006173972923 1101 52-53 0.5092810238351007 1101 54-55 0.5417195756055904 1101 56-57 0.5351352784105572 1101 58-59 0.47742250008076326 1101 60-61 0.47820781103016685 1101 62-63 0.4081407282741196 1101 64-65 0.44686097178301054 1101 66-67 0.4383788208634911 1101 68-69 0.421964089126007 1101 70-71 0.4386522026725004 1101 72-73 0.4269063583838104 1101 74-75 0.38094480325138136 1101 76-77 0.4279066908335736 1101 78-79 0.40588004118283294 1101 80-81 0.41257091929389134 1101 82-83 0.4179563885915272 1101 84-85 0.44433622194116396 1101 86-87 0.4397938953593936 1101 88-89 0.4828363548439647 1101 90-91 0.4139752805457455 1101 92-93 0.4384564220555127 1101 94-95 0.4705112043337145 1101 96-97 0.4858525601457018 1101 98-99 0.4693788614592833 1101 100-101 0.5756446594475371 1102 1 0.3493500031218275 1102 2 0.37085491375925983 1102 3 0.36861783038241214 1102 4 0.23689142848724742 1102 5 0.26507926176661556 1102 6 0.26444752623861234 1102 7 0.24496059150667548 1102 8 0.26094221144713003 1102 9 0.3529126381995127 1102 10-11 0.299921057173016 1102 12-13 0.2778215641339372 1102 14-15 0.3928900893914147 1102 16-17 0.413596276402032 1102 18-19 0.4113684363613146 1102 20-21 0.4267557022656234 1102 22-23 0.3926111740812459 1102 24-25 0.4089017134183237 1102 26-27 0.3925674641706749 1102 28-29 0.45911100472763877 1102 30-31 0.44210907681637934 1102 32-33 0.4389166980385113 1102 34-35 0.49730334855066616 1102 36-37 0.5659863788379766 1102 38-39 0.5001267829309484 1102 40-41 0.4764431614725524 1102 42-43 0.5330034012049794 1102 44-45 0.48002561962569956 1102 46-47 0.49993388094780755 1102 48-49 0.42220038467231547 1102 50-51 0.3576896659750304 1102 52-53 0.3236534502241213 1102 54-55 0.3631813280823408 1102 56-57 0.37670085969946854 1102 58-59 0.3934064230651586 1102 60-61 0.3927834124195684 1102 62-63 0.36509736988059416 1102 64-65 0.37315016675196944 1102 66-67 0.2871900509318408 1102 68-69 0.2467359876469999 1102 70-71 0.25727539766593566 1102 72-73 0.3041482883807305 1102 74-75 0.31966633007980505 1102 76-77 0.33190331406565576 1102 78-79 0.31268158958340564 1102 80-81 0.29543842803636977 1102 82-83 0.32778926774027894 1102 84-85 0.28942803772117287 1102 86-87 0.3241944871914555 1102 88-89 0.32364486294722283 1102 90-91 0.2708470865333723 1102 92-93 0.3317286329142384 1102 94-95 0.2988005584436202 1102 96-97 0.35050961524395774 1102 98-99 0.39672858956272705 1102 100-101 0.45923107677404573 1103 1 0.2700592604078338 1103 2 0.3033017387396342 1103 3 0.24201677197137883 1103 4 0.16219261847091815 1103 5 0.20488928578109977 1103 6 0.2038921181540161 1103 7 0.2353976099438242 1103 8 0.16736012494275343 1103 9 0.1870063914212139 1103 10-11 0.22989445515418794 1103 12-13 0.20797557495074415 1103 14-15 0.3569681657099508 1103 16-17 0.392950696420975 1103 18-19 0.39964189077293355 1103 20-21 0.3910640936853511 1103 22-23 0.4089309016447302 1103 24-25 0.4002396980909282 1103 26-27 0.38583189790309547 1103 28-29 0.39450952438764375 1103 30-31 0.3813676687166705 1103 32-33 0.4563573911751604 1103 34-35 0.4397563428530944 1103 36-37 0.49873057401766374 1103 38-39 0.476320362359715 1103 40-41 0.4274216835891522 1103 42-43 0.41223030636913904 1103 44-45 0.43997773318752564 1103 46-47 0.5090912584987777 1103 48-49 0.4285008017802241 1103 50-51 0.3776249711151607 1103 52-53 0.2930544446763719 1103 54-55 0.38662447775175934 1103 56-57 0.35789105337552485 1103 58-59 0.3826036417076111 1103 60-61 0.3917414412415283 1103 62-63 0.4022621243041158 1103 64-65 0.33611834177302313 1103 66-67 0.3002763322199229 1103 68-69 0.327285638302115 1103 70-71 0.3291927520914584 1103 72-73 0.3165503188055254 1103 74-75 0.3403731299264052 1103 76-77 0.36240973603165827 1103 78-79 0.3873815961650351 1103 80-81 0.39666504347359677 1103 82-83 0.3449212048579611 1103 84-85 0.3671659022269864 1103 86-87 0.39075931508092054 1103 88-89 0.38704905582381244 1103 90-91 0.33657937907206303 1103 92-93 0.3550257120055491 1103 94-95 0.38000398708079786 1103 96-97 0.39267612334727175 1103 98-99 0.4139834724479243 1103 100-101 0.4573199036889335 1104 1 0.19766817028869355 1104 2 0.22414693416794762 1104 3 0.2209729819306574 1104 4 0.1657999267761241 1104 5 0.1453261971654527 1104 6 0.211244825699886 1104 7 0.23054049319414815 1104 8 0.18690683565581878 1104 9 0.2648449671336408 1104 10-11 0.2470919963768452 1104 12-13 0.22183191944930059 1104 14-15 0.3719886579657512 1104 16-17 0.3542902671094055 1104 18-19 0.3065268422082852 1104 20-21 0.3369173086757016 1104 22-23 0.30567421645172743 1104 24-25 0.3029090926815883 1104 26-27 0.3299844275806407 1104 28-29 0.28648180293835424 1104 30-31 0.2915572482195046 1104 32-33 0.34689969868814785 1104 34-35 0.346094231231568 1104 36-37 0.32166890674932347 1104 38-39 0.3756930546207897 1104 40-41 0.3174984311341902 1104 42-43 0.3962133106332928 1104 44-45 0.4191648314741343 1104 46-47 0.4254277257069745 1104 48-49 0.31859655693406097 1104 50-51 0.3356881823698359 1104 52-53 0.3332943111905564 1104 54-55 0.3603711563390348 1104 56-57 0.346066904488751 1104 58-59 0.33169566118350247 1104 60-61 0.3432295878023126 1104 62-63 0.3742533657517413 1104 64-65 0.34834217646675114 1104 66-67 0.2939192986576131 1104 68-69 0.3589758692277698 1104 70-71 0.31017485045438775 1104 72-73 0.3319552270516226 1104 74-75 0.3473258431330777 1104 76-77 0.3152694398797635 1104 78-79 0.31177916092921976 1104 80-81 0.32279269515514386 1104 82-83 0.3331495418563648 1104 84-85 0.3250124581003675 1104 86-87 0.34703790002342316 1104 88-89 0.3175798924117963 1104 90-91 0.3590079578808414 1104 92-93 0.3940692473099183 1104 94-95 0.34989633937496123 1104 96-97 0.40255487533822887 1104 98-99 0.3944348397307742 1104 100-101 0.45850281627550515 1105 1 0.1712876299085977 1105 2 0.1956286446420421 1105 3 0.2077075715887986 1105 4 0.09306814298765431 1105 5 0.11927062020158985 1105 6 0.12225908578093936 1105 7 0.15721980930720036 1105 8 0.15437857129447252 1105 9 0.16793750969138443 1105 10-11 0.1547992323291183 1105 12-13 0.12935036379900566 1105 14-15 0.20459122276997022 1105 16-17 0.18749521959050242 1105 18-19 0.17278424144107163 1105 20-21 0.21396189275559152 1105 22-23 0.19255191537801153 1105 24-25 0.1611554222640592 1105 26-27 0.2188671582754651 1105 28-29 0.18991095839547256 1105 30-31 0.16670575572211987 1105 32-33 0.24903395298900932 1105 34-35 0.24447133503385743 1105 36-37 0.18520149483309467 1105 38-39 0.21136745113345512 1105 40-41 0.25158442357785304 1105 42-43 0.30687547444948393 1105 44-45 0.12256333854062973 1105 46-47 0.22157737737215655 1105 48-49 0.14308488221531235 1105 50-51 0.1157148508160617 1105 52-53 0.11856995880646082 1105 54-55 0.14204165851423767 1105 56-57 0.1122148399741576 1105 58-59 0.10799145945836841 1105 60-61 0.12507859218889195 1105 62-63 0.1282072294028609 1105 64-65 0.1041929051542354 1105 66-67 0.08632024126440996 1105 68-69 0.12479956569372774 1105 70-71 0.0868535638890151 1105 72-73 0.08910650916151042 1105 74-75 0.12418093979525935 1105 76-77 0.12348226438574983 1105 78-79 0.09739456049727124 1105 80-81 0.056128698408087985 1105 82-83 0.08433948678732861 1105 84-85 0.0384522381423551 1105 86-87 0.044927872113845524 1105 88-89 0.039852449534155454 1105 90-91 0.035809600958632615 1105 92-93 0.04248969418704718 1105 94-95 0.0525112068021194 1105 96-97 0.06366589166998438 1105 98-99 0.08040613396538987 1105 100-101 0.09339652789929431 1106 1 0.14351578274715848 1106 2 0.1665829789215465 1106 3 0.15006332857459626 1106 4 0.09545105482453664 1106 5 0.11911252949089857 1106 6 0.1149194473845867 1106 7 0.1403067039384709 1106 8 0.13474476048277495 1106 9 0.1517358409953431 1106 10-11 0.1478618918898249 1106 12-13 0.1337033207112981 1106 14-15 0.19555993973533248 1106 16-17 0.1628789459704052 1106 18-19 0.1545873087083791 1106 20-21 0.19268073896220272 1106 22-23 0.17105175431645847 1106 24-25 0.18340603962555235 1106 26-27 0.18803882426662 1106 28-29 0.15439183115642408 1106 30-31 0.1405446854723067 1106 32-33 0.14691737217596312 1106 34-35 0.18272403726405884 1106 36-37 0.15680514855088035 1106 38-39 0.2001674513308842 1106 40-41 0.14888219242398293 1106 42-43 0.22789008176160053 1106 44-45 0.1809930425702575 1106 46-47 0.25752139712498945 1106 48-49 0.19771133055201773 1106 50-51 0.16360049006888744 1106 52-53 0.13710401599327326 1106 54-55 0.1377492622275298 1106 56-57 0.16292032831299963 1106 58-59 0.13965912833434402 1106 60-61 0.20326211302810293 1106 62-63 0.16634474352932926 1106 64-65 0.09701165645137166 1106 66-67 0.16118572700789002 1106 68-69 0.1582396330364233 1106 70-71 0.12556642531436069 1106 72-73 0.11653456407798757 1106 74-75 0.13096258138619277 1106 76-77 0.1050631542181506 1106 78-79 0.1187688498033097 1106 80-81 0.15132251775229122 1106 82-83 0.09591207609809516 1106 84-85 0.12628051673972607 1106 86-87 0.15700993854318668 1106 88-89 0.2290034052245815 1106 90-91 0.2405688156889667 1106 92-93 0.25893744660241325 1106 94-95 0.2690050927368972 1106 96-97 0.22612231587717702 1106 98-99 0.24056073236723918 1106 100-101 0.2966367439436901 1107 1 0.05330788230411798 1107 2 0.08917877499234095 1107 3 0.12416133790695483 1107 4 0.08259638909352418 1107 5 -0.0286516109925401 1107 6 0.04784229433064269 1107 7 0.0983450669417536 1107 8 0.1138715908930692 1107 9 0.12257875061542478 1107 10-11 0.06546218722298391 1107 12-13 0.11832676907932438 1107 14-15 0.19484189841397637 1107 16-17 0.16778793025037686 1107 18-19 0.11651635275724459 1107 20-21 0.15719180445686476 1107 22-23 0.16893643016073412 1107 24-25 0.15260107084022678 1107 26-27 0.1395167301274043 1107 28-29 0.08927055250989469 1107 30-31 0.11519844962106873 1107 32-33 0.13448298996094366 1107 34-35 0.15463788258065136 1107 36-37 0.19602368806954473 1107 38-39 0.21448503338965708 1107 40-41 0.14156963439984338 1107 42-43 0.1809612099509863 1107 44-45 0.20738083936430485 1107 46-47 0.21332157761293757 1107 48-49 0.23382073858244468 1107 50-51 0.1278978747899373 1107 52-53 0.206450093873201 1107 54-55 0.21576559573637866 1107 56-57 0.24671151293740223 1107 58-59 0.20167025906516756 1107 60-61 0.23200189525241655 1107 62-63 0.18188425620446935 1107 64-65 0.23448308282732455 1107 66-67 0.16261756837602093 1107 68-69 0.173130591067725 1107 70-71 0.14009687500478663 1107 72-73 0.10222221200052672 1107 74-75 0.11161412691404138 1107 76-77 0.11298312929460508 1107 78-79 0.13569814223009047 1107 80-81 0.0875790896950619 1107 82-83 0.0520117977595973 1107 84-85 0.05418717402913842 1107 86-87 0.016344139375799926 1107 88-89 0.04030869176361662 1107 90-91 0.04334111974817745 1107 92-93 0.09606490653244393 1107 94-95 -0.004754850972979341 1107 96-97 0.037818612821247655 1107 98-99 0.06328752191345899 1107 100-101 0.10316364789159138 1108 1 0.04649268242962279 1108 2 0.06694336091056385 1108 3 0.09317116405964043 1108 4 0.030058917081660752 1108 5 -0.03924075230943913 1108 6 0.020866724228390865 1108 7 0.07129997217428752 1108 8 0.06027342206579078 1108 9 0.046862824832111016 1108 10-11 0.032683981601117296 1108 12-13 0.04677162710639493 1108 14-15 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-0.6491942963082522 2214 46-47 -0.572066936436137 2214 48-49 -0.4706941700917824 2214 50-51 -0.5156257455870445 2214 52-53 -0.5093678070170569 2214 54-55 -0.4954308927689155 2214 56-57 -0.41468494776119513 2214 58-59 -0.3575752011006159 2214 60-61 -0.33706416373770054 2214 62-63 -0.3623459731850147 2214 64-65 -0.3095603706396872 2214 66-67 -0.2724512055583119 2214 68-69 -0.24467582419369194 2214 70-71 -0.2537411979199504 2214 72-73 -0.22054063132688384 2214 74-75 -0.23543903920289466 2214 76-77 -0.19819783285402792 2214 78-79 -0.2508103596978444 2214 80-81 -0.26388305929361167 2214 82-83 -0.2202268081290839 2214 84-85 -0.19405848858067287 2214 86-87 -0.2598331251665087 2214 88-89 -0.2623251540532827 2214 90-91 -0.269474219792297 2214 92-93 -0.27993820883760634 2214 94-95 -0.25629456548260876 2214 96-97 -0.3273986375707807 2214 98-99 -0.4217379441297098 2214 100-101 -0.3867702597019367 2215 1 -0.41086293990917966 2215 2 -0.4164942438614574 2215 3 -0.34766709144277286 2215 4 -0.23273439379841676 2215 5 -0.2560602930256479 2215 6 -0.2923521218801426 2215 7 -0.31058081102373336 2215 8 -0.3675442283285477 2215 9 -0.4461122866071108 2215 10-11 -0.4173757837946752 2215 12-13 -0.43785257976999503 2215 14-15 -0.5564205674456915 2215 16-17 -0.550981847751757 2215 18-19 -0.6046557569634459 2215 20-21 -0.5554344836048912 2215 22-23 -0.5580040557950312 2215 24-25 -0.563809424038503 2215 26-27 -0.5573486266464442 2215 28-29 -0.5812003114880113 2215 30-31 -0.6137194628554283 2215 32-33 -0.6288330579015664 2215 34-35 -0.591123631503855 2215 36-37 -0.5684217992106539 2215 38-39 -0.6445316593303829 2215 40-41 -0.6057004736182563 2215 42-43 -0.5675518661942789 2215 44-45 -0.7229861751438804 2215 46-47 -0.6040419342351981 2215 48-49 -0.5014815807410002 2215 50-51 -0.5125504809073078 2215 52-53 -0.580096664531041 2215 54-55 -0.5882328077163521 2215 56-57 -0.5997234794377846 2215 58-59 -0.5664968558522361 2215 60-61 -0.49292325694406003 2215 62-63 -0.5237871233285958 2215 64-65 -0.5150032168043026 2215 66-67 -0.5129000463223079 2215 68-69 -0.5125663717243256 2215 70-71 -0.48168645906278584 2215 72-73 -0.46277676449171423 2215 74-75 -0.405196893605158 2215 76-77 -0.4181127111113838 2215 78-79 -0.41555706413263493 2215 80-81 -0.43119248367536755 2215 82-83 -0.4008713978133045 2215 84-85 -0.40803096341538847 2215 86-87 -0.4380518387758343 2215 88-89 -0.516928766340488 2215 90-91 -0.5041952801530414 2215 92-93 -0.5534870053508172 2215 94-95 -0.5639052427821305 2215 96-97 -0.6449315641941809 2215 98-99 -0.635924818971823 2215 100-101 -0.713547330534194 2216 1 -0.8307210798644107 2216 2 -0.7074543491365048 2216 3 -1.7512112082585922 2216 4 -0.7557029425799087 2216 5 -0.6250879032077208 2216 6 -0.692199900467358 2216 7 -0.6239698132789684 2216 8 -0.5765129761210943 2216 9 -0.8060656741795285 2216 10-11 -0.743525384462437 2216 12-13 -0.6130395507545217 2216 14-15 -0.9343166214175369 2216 16-17 -0.9190557513931168 2216 18-19 -0.8994540453734317 2216 20-21 -1.0971612003232352 2216 22-23 -0.8411690211650793 2216 24-25 -0.8516489747677554 2216 26-27 -0.7535582082411096 2216 28-29 -0.7989199019218205 2216 30-31 -0.8023545138363772 2216 32-33 -0.9497392539493319 2216 34-35 -0.9620588514185968 2216 36-37 -0.9009532852809556 2216 38-39 -0.8962078198362917 2216 40-41 -0.9333902319114031 2216 42-43 -0.9404454467017374 2216 44-45 -0.8802233075245312 2216 46-47 -0.6287608348387295 2216 48-49 -0.6872464513014123 2216 50-51 -0.8040050007789787 2216 52-53 -0.9591502373511389 2216 54-55 -0.9022000944644333 2216 56-57 -0.8927136896124637 2216 58-59 -0.9295217724466909 2216 60-61 -0.6748530982100718 2216 62-63 -0.6017744464776342 2216 64-65 -0.7373012895101567 2216 66-67 -0.7627886767916863 2216 68-69 -0.570781173897231 2216 70-71 -0.6739994150019157 2216 72-73 -0.7160444456991684 2216 74-75 -0.8418155551824604 2216 76-77 -0.7935806788997866 2216 78-79 -0.6531341029029889 2216 80-81 -0.9630293352514059 2216 82-83 -0.7607144566224804 2216 84-85 -0.788864912619573 2216 86-87 -0.7981624508652665 2216 88-89 -0.6716985824716595 2216 90-91 -0.7284140825682499 2216 92-93 -0.6754131897943729 2216 94-95 -0.8034908030515524 2216 96-97 -1.0177697781031867 2216 98-99 -0.8145573422052195 2216 100-101 -0.9936937625346403 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 102.0 3 52.0 4 96.0 5 213.0 6 462.0 7 1054.0 8 2371.0 9 4075.0 10 6744.0 11 8184.0 12 9896.0 13 15223.0 14 25036.0 15 41729.0 16 48476.0 17 56929.0 18 41956.0 19 26700.0 20 23226.0 21 21140.0 22 20610.0 23 22365.0 24 27308.0 25 35243.0 26 46799.0 27 62190.0 28 81943.0 29 101474.0 30 130138.0 31 168275.0 32 220140.0 33 291571.0 34 395518.0 35 581963.0 36 945929.0 37 1617233.0 38 1479556.0 39 244611.0 40 147.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.588352787701343 31.2265985891221 14.663281414500965 24.521767208675595 2 26.796872509032315 27.097952214267778 24.913298713060946 21.19187656363896 3 24.911979137972196 25.49820211925881 22.32087121003434 27.26894753273465 4 27.317382622974474 24.479713081728427 23.40766279933659 24.79524149596051 5 26.260272553705242 26.10146531959423 22.70636382268656 24.931898304013963 6 26.340837331810878 24.014974401859153 23.482781068644332 26.161407197685644 7 27.345910678205758 24.910112848191503 22.555959820825137 25.188016652777602 8 27.24476275280875 23.286678653915853 23.910257530950858 25.55830106232454 9 25.005167719872706 22.885058303780244 24.888120884831174 27.221653091515876 10-11 26.61007713455479 23.808166598767652 23.302964133599993 26.278792133077566 12-13 26.365229719356364 24.011907429104518 23.59475074100056 26.028112110538554 14-15 25.740673459310614 24.143330144797527 23.787892388606068 26.328104007285784 16-17 26.006206846600772 24.144013297531234 24.129630361481823 25.720149494386174 18-19 24.695765643059016 23.68586022224942 25.681246517206564 25.937127617485007 20-21 24.485780653320262 24.132296860861768 25.73133703861664 25.65058544720133 22-23 24.37711529429118 24.091888009376675 26.76452841819878 24.76646827813337 24-25 24.21764687820503 23.32403168242007 27.92236211590472 24.535959323470173 26-27 24.177073986151814 23.33453197640571 28.16650584770083 24.321888189741646 28-29 24.3770859113779 23.57675411952117 27.976375256237368 24.06978471286356 30-31 24.162580785630684 23.411883473442195 28.142301270158253 24.283234470768864 32-33 24.159417289817043 23.4859535717649 28.10094411707798 24.253685021340075 34-35 24.348018864417984 23.4586715367866 27.926137820260898 24.267171778534518 36-37 24.38259284925876 23.419578879540026 27.771462059682506 24.426366211518705 38-39 24.53265374572644 23.52064010088917 27.636576555637944 24.31012959774645 40-41 24.803259562800985 23.75551251535835 26.879683810772466 24.561544111068194 42-43 24.955967476645704 23.69740531143484 26.604466404123105 24.742160807796353 44-45 25.104239557716635 23.85652352829435 26.135565122305643 24.903671791683372 46-47 25.181168216647738 24.31215725763354 25.437956180250943 25.06871834546778 48-49 25.497551889122988 24.221841289075417 24.966161902496623 25.314444919304968 50-51 25.311607700411166 24.389659827326867 24.793021203963665 25.505711268298302 52-53 25.565029749465122 24.476517689909482 24.57168160028748 25.386770960337916 54-55 25.56057089237524 24.365024225477423 24.52865766952068 25.54574721262666 56-57 25.428024570579744 24.39300410464607 24.56648082463734 25.612490500136854 58-59 25.584091914454 24.44221313865782 24.355511507303785 25.618183439584396 60-61 25.598526270601646 24.314206476963722 24.2999704554807 25.787296796953935 62-63 25.599767698687625 24.434448703824202 24.289980264966296 25.675803332521873 64-65 25.625110461389607 24.678613367433183 24.182181701878903 25.51409446929831 66-67 25.699060446305634 24.54414339183115 24.16584345042103 25.59095271144219 68-69 25.668935076543224 24.662085478714506 24.058171116390568 25.610808328351702 70-71 25.72883681922555 24.7556195224068 23.908462760060914 25.607080898306737 72-73 25.703636297124135 24.705645647648623 23.923545953480676 25.667172101746566 74-75 25.606345887478703 24.724586567657987 24.151259733826024 25.51780781103728 76-77 25.689435522359048 24.828802204189756 23.979811871393565 25.501950402057627 78-79 25.710959988258587 24.702670627679264 23.99441019457806 25.591959189484093 80-81 25.5703482243533 24.791906474159557 24.05838386583758 25.579361435649567 82-83 25.7097017598347 24.960994096665885 23.946693695088722 25.38261044841069 84-85 25.667976711553735 24.906756449624094 23.935013722635716 25.49025311618645 86-87 25.47834412627968 24.992823148570668 24.010307045831652 25.518525679318003 88-89 25.615568106499232 24.99413993016692 23.885115745836853 25.505176217496995 90-91 25.601637628491574 24.89931937369687 23.848343213834898 25.65069978397666 92-93 25.445877834681458 25.012410695514625 23.91127825535959 25.63043321444433 94-95 25.623916823186242 25.121533604641282 23.772787711558532 25.481761860613943 96-97 25.52326627312474 25.066793190095353 23.81181358891301 25.598126947866895 98-99 25.53884426234867 25.124493612850152 23.83166699098069 25.504995133820486 100-101 25.537842933820155 25.15400416711109 23.657780042235267 25.6503728568335 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 100.0 1 70.0 2 37.0 3 28.5 4 23.5 5 21.5 6 21.5 7 25.0 8 23.5 9 24.5 10 31.5 11 42.5 12 50.5 13 74.0 14 113.0 15 157.0 16 526.0 17 695.0 18 543.5 19 683.0 20 922.5 21 1333.5 22 1944.5 23 2817.0 24 4063.0 25 5669.0 26 7783.5 27 10742.0 28 14448.5 29 19000.0 30 24594.5 31 30794.0 32 38035.0 33 46393.0 34 54839.0 35 64247.0 36 75943.5 37 87871.0 38 99755.5 39 113283.5 40 126511.5 41 138702.5 42 153615.5 43 172098.0 44 193592.0 45 214152.5 46 233872.0 47 253767.0 48 272693.5 49 286158.5 50 292694.0 51 298088.0 52 305196.0 53 313675.0 54 313616.0 55 308420.0 56 293008.0 57 267580.5 58 259439.0 59 254029.0 60 219986.5 61 181912.0 62 151996.5 63 118373.0 64 93167.5 65 77476.0 66 60115.0 67 42177.5 68 31452.5 69 25670.5 70 23092.0 71 23091.0 72 22953.0 73 19147.0 74 14205.5 75 12164.0 76 10248.0 77 7738.5 78 5475.5 79 3208.5 80 1434.0 81 1217.5 82 1086.0 83 466.5 84 99.0 85 25.5 86 22.0 87 16.5 88 9.0 89 4.0 90 3.0 91 3.0 92 1.5 93 2.0 94 2.0 95 0.5 96 0.5 97 1.0 98 2.0 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08528390578838985 2 0.012311440663336897 3 7.63955745218996E-4 4 0.0 5 0.0045837344713139765 6 0.0018805064497698365 7 0.005362381673171799 8 0.0 9 0.0 10-11 0.0 12-13 2.9382913277653695E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0054284932280465195 28-29 0.0 30-31 8.080301151354766E-5 32-33 0.0 34-35 0.0 36-37 3.81977872609498E-4 38-39 0.0 40-41 0.0017629747966592213 42-43 8.888331266490242E-4 44-45 0.0 46-47 0.0013001939125361758 48-49 0.0 50-51 2.2037184958240268E-5 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 6.023497221919008E-4 68-69 0.0 70-71 3.893236009289114E-4 72-73 0.0 74-75 1.4691456638826848E-5 76-77 5.142009823589396E-5 78-79 0.0 80-81 2.9382913277653695E-5 82-83 2.2037184958240266E-4 84-85 5.876582655530738E-4 86-87 0.001043093421356706 88-89 2.571004911794698E-4 90-91 7.345728319413422E-5 92-93 7.051899186636886E-4 94-95 3.011748610959504E-4 96-97 4.7747234076187244E-4 98-99 3.672864159706711E-5 100-101 6.317326354695543E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6806677.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.22206255366388 #Duplication Level Percentage of deduplicated Percentage of total 1 87.32908437964088 53.46466666644565 2 7.085313964313903 8.67555069471148 3 1.9376385801242826 3.5587869107628385 4 0.8423957246873559 2.062928150069933 5 0.5588496277383819 1.7106963433745497 6 0.37135944860362585 1.3641234835383187 7 0.29024963796948633 1.2438777044362332 8 0.23086267353460144 1.1307111232353162 9 0.18464602590394275 1.017396949335896 >10 1.048883488415355 11.158048414784872 >50 0.04452650882695683 1.878180802739538 >100 0.07102890408838204 9.79988256048691 >500 0.004584947591388743 1.8192286010674692 >1k 4.8007380193531706E-4 0.44693588777580645 >5k 0.0 0.0 >10k+ 9.60147596588319E-5 0.6689857072352959 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14983 0.22012209481954265 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGC 10249 0.15057273909133634 TruSeq Adapter, Index 23 (97% over 38bp) ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 10183 0.14960310295317378 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10108 0.14850124370526177 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.4074369916480536E-5 0.0 0.0 0.0 1.4691456638826846E-5 2 4.4074369916480536E-5 0.0 0.0 0.0 1.4691456638826846E-5 3 4.4074369916480536E-5 0.0 0.0 0.0 1.4691456638826846E-5 4 4.4074369916480536E-5 0.0 0.0 0.0 2.9382913277653692E-5 5 7.345728319413422E-5 0.0 0.0 1.4691456638826846E-5 2.9382913277653692E-5 6 1.322231097494416E-4 0.0 0.0 1.4691456638826846E-5 7.345728319413422E-5 7 1.616060230270953E-4 0.0 0.0 1.4691456638826846E-5 7.345728319413422E-5 8 2.3506330622122953E-4 0.0 0.0 2.9382913277653692E-5 8.814873983296107E-5 9 3.6728641597067114E-4 0.0 0.0 2.9382913277653692E-5 8.814873983296107E-5 10-11 5.729668089142471E-4 0.0 0.0 3.672864159706711E-5 1.0284019647178792E-4 12-13 8.006843868160631E-4 1.4691456638826846E-5 0.0 5.142009823589396E-5 1.1018592479120134E-4 14-15 0.0010210562363984657 1.4691456638826846E-5 0.0 6.61115548747208E-5 1.1753165311061477E-4 16-17 0.0012928481842167624 1.4691456638826846E-5 0.0 7.345728319413422E-5 1.322231097494416E-4 18-19 0.0019025436347280766 1.4691456638826846E-5 0.0 7.345728319413422E-5 1.5426029470768187E-4 20-21 0.0028207596746547543 1.4691456638826846E-5 0.0 7.345728319413422E-5 1.616060230270953E-4 22-23 0.004172373685426825 1.4691456638826846E-5 0.0 7.345728319413422E-5 1.6895175134650872E-4 24-25 0.005678247990906576 1.4691456638826846E-5 0.0 7.345728319413422E-5 1.90988936304749E-4 26-27 0.006919676076887444 1.4691456638826846E-5 0.0 7.345728319413422E-5 1.90988936304749E-4 28-29 0.00810968406463242 1.4691456638826846E-5 0.0 7.345728319413422E-5 1.90988936304749E-4 30-31 0.00982858449137516 1.4691456638826846E-5 0.0 8.814873983296107E-5 2.0568039294357584E-4 32-33 0.012201254738545695 1.4691456638826846E-5 0.0 8.814873983296107E-5 2.644462194988832E-4 34-35 0.01553621539555939 1.4691456638826846E-5 0.0 1.0284019647178792E-4 2.644462194988832E-4 36-37 0.019760009179222108 1.4691456638826846E-5 0.0 1.0284019647178792E-4 2.7913767613771005E-4 38-39 0.025151773765671563 1.4691456638826846E-5 0.0 1.0284019647178792E-4 2.864834044571235E-4 40-41 0.03323207491702633 1.4691456638826846E-5 0.0 1.0284019647178792E-4 2.938291327765369E-4 42-43 0.04315615387655386 2.2037184958240268E-5 0.0 1.0284019647178792E-4 3.1586631773477717E-4 44-45 0.05556308900804313 2.9382913277653692E-5 0.0 1.0284019647178792E-4 3.3790350269301744E-4 46-47 0.07253172142588814 2.9382913277653692E-5 0.0 1.0284019647178792E-4 3.599406876512577E-4 48-49 0.09433384307790718 2.9382913277653692E-5 0.0 1.1753165311061477E-4 3.7463214429008456E-4 50-51 0.1201687695772842 2.9382913277653692E-5 0.0 1.1753165311061477E-4 4.0401505756773826E-4 52-53 0.150940025507307 2.9382913277653692E-5 0.0 1.1753165311061477E-4 4.113607858871517E-4 54-55 0.19008541172146115 4.4074369916480536E-5 0.0 1.1753165311061477E-4 4.407436991648054E-4 56-57 0.23621658556737746 4.4074369916480536E-5 0.0 1.1753165311061477E-4 4.848180690812859E-4 58-59 0.2898257108424566 4.4074369916480536E-5 0.0 1.1753165311061477E-4 4.995095257201127E-4 60-61 0.3541816366488376 4.4074369916480536E-5 0.0 1.2487738143002818E-4 5.21546710678353E-4 62-63 0.4269631128375858 4.4074369916480536E-5 0.0 1.4691456638826845E-4 5.656210805948335E-4 64-65 0.5106823784939406 4.4074369916480536E-5 0.0 1.4691456638826845E-4 5.876582655530738E-4 66-67 0.6058756717852192 4.4074369916480536E-5 0.0 1.616060230270953E-4 5.950039938724872E-4 68-69 0.7139166439071517 4.4074369916480536E-5 0.0 1.7629747966592214E-4 6.096954505113141E-4 70-71 0.8329541713232462 4.4074369916480536E-5 0.0 1.90988936304749E-4 6.243869071501409E-4 72-73 0.9677629774411214 4.4074369916480536E-5 0.0 2.0568039294357584E-4 6.831527337054483E-4 74-75 1.1130247549575218 4.4074369916480536E-5 0.0 2.0568039294357584E-4 7.198813753025154E-4 76-77 1.276812459295483 4.4074369916480536E-5 0.0 2.0568039294357584E-4 7.198813753025154E-4 78-79 1.4575173759530533 4.4074369916480536E-5 0.0 2.0568039294357584E-4 7.198813753025154E-4 80-81 1.654030299954001 4.4074369916480536E-5 0.0 2.0568039294357584E-4 7.492642885801691E-4 82-83 1.8678277226905289 5.8765826555307384E-5 0.0 2.203718495824027E-4 7.786472018578228E-4 84-85 2.0977637105448075 7.345728319413422E-5 0.0 2.203718495824027E-4 7.786472018578228E-4 86-87 2.3462623538622447 7.345728319413422E-5 0.0 2.3506330622122953E-4 7.786472018578228E-4 88-89 2.6136248275039344 7.345728319413422E-5 0.0 2.3506330622122953E-4 7.933386584966497E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 5700 0.0 90.08761 1 TCGACGG 6230 0.0 82.96587 2 CGACGGC 8125 0.0 63.72607 3 ACGGCGC 8145 0.0 63.336304 5 GACGGCG 8400 0.0 61.58325 4 TATCTCG 4495 0.0 57.811775 2 ATCTCGA 10875 0.0 52.15877 1 CGGCGCG 10155 0.0 50.844925 6 ATATCTC 5140 0.0 50.59882 1 TCTCGAG 13370 0.0 42.39042 2 CTCGAGG 19235 0.0 29.832438 3 CGCGCCG 35630 0.0 29.008282 9 GGCGCGC 37480 0.0 27.53923 7 GCGCGCC 37740 0.0 27.487146 8 TCGAGGG 22085 0.0 25.87511 4 CGAGGGC 22660 0.0 25.23949 5 ATCCATA 10825 0.0 24.747429 16-17 TCCATAT 10790 0.0 24.343475 18-19 CCATATT 11105 0.0 24.273153 18-19 GGGCGCG 25175 0.0 22.65961 8 >>END_MODULE