FastQCFastQC Report
Tue 31 May 2016
SRR2048264_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048264_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2042748
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT85660.41933708905846434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG64250.093.899181
TCGACGG65750.091.75252
ACGGCGC70100.085.8471765
TATCTCG25350.085.4472
CGACGGC71400.084.417183
GACGGCG71550.084.24024
ATCTCGA26350.081.294913
CGGCGCG82200.077.7751166
ATATCTC28750.075.345651
TCTCGAG29250.071.123924
CTCGAGG30450.068.009035
AGGGCGC33900.062.068539
CGAGGGC34450.060.801857
TCGAGGG35400.058.9018176
CGCGCCG111850.057.9647679
GAGGGCG36300.057.4414448
GGCGCGC116950.055.437027
GCGCGCC123200.052.8174328
TCGCCGG9350.041.659322
TCCATAT75700.039.75118318-19
Produced by FastQC (version 0.11.5)