##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048264_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2042748 Sequences flagged as poor quality 0 Sequence length 101 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.25803770215416 31.0 30.0 34.0 28.0 34.0 2 30.73844057123052 31.0 30.0 34.0 27.0 34.0 3 31.374429934578323 31.0 31.0 34.0 28.0 34.0 4 34.33934288517233 37.0 35.0 37.0 30.0 37.0 5 34.33429429376507 35.0 35.0 37.0 30.0 37.0 6 34.102141331187205 35.0 35.0 37.0 30.0 37.0 7 34.209864359186746 35.0 35.0 37.0 30.0 37.0 8 33.95124900379293 35.0 35.0 37.0 29.0 37.0 9 36.18963278877277 39.0 35.0 39.0 31.0 39.0 10-11 35.3284259732478 38.0 34.5 39.0 28.0 39.0 12-13 35.05494755104399 37.0 34.0 39.0 27.0 39.0 14-15 36.026561279218 39.0 34.0 40.0 27.0 41.0 16-17 35.92570106542755 38.5 34.0 40.0 27.0 41.0 18-19 35.97267112732456 39.0 34.0 40.0 27.0 41.0 20-21 36.30349215860204 39.0 34.5 40.5 28.5 41.0 22-23 35.95573536236481 39.0 34.0 40.0 27.0 41.0 24-25 35.65187213498679 38.5 34.0 40.0 26.5 41.0 26-27 35.1853143412697 38.0 33.0 40.0 25.0 41.0 28-29 35.01026509388333 38.0 33.0 40.0 25.0 41.0 30-31 34.80047514426645 38.0 33.0 40.0 25.0 41.0 32-33 34.73098419384085 38.0 33.0 40.0 25.0 41.0 34-35 34.578409329001914 38.0 33.0 40.0 24.5 41.0 36-37 34.37199253162896 37.0 32.5 40.0 24.0 41.0 38-39 34.54504495904536 38.0 32.5 40.0 24.0 41.0 40-41 34.313321320104095 38.0 32.0 40.0 23.0 41.0 42-43 34.056112648256175 37.0 31.5 40.0 22.5 41.0 44-45 33.77902927820759 37.0 31.0 40.0 22.0 41.0 46-47 33.65081277768966 37.0 31.0 40.0 22.0 41.0 48-49 33.56850771607658 37.0 31.0 40.0 22.0 41.0 50-51 33.44573290489086 36.0 30.5 40.0 22.0 41.0 52-53 33.22496754372052 36.0 30.0 39.5 22.0 41.0 54-55 33.004480973668805 35.5 30.0 39.0 21.0 41.0 56-57 33.39333902174913 36.0 31.0 39.5 22.0 41.0 58-59 33.3455210823851 35.5 31.0 39.0 22.0 41.0 60-61 33.242144160709 35.0 31.0 39.0 23.0 41.0 62-63 33.05003040022558 35.0 31.0 39.0 22.5 41.0 64-65 32.77684325232481 35.0 31.0 39.0 22.5 40.0 66-67 32.46111463577495 35.0 31.0 38.0 22.0 40.0 68-69 32.21712394284562 35.0 31.0 37.0 22.0 40.0 70-71 31.96204646877637 35.0 30.0 37.0 22.5 39.0 72-73 31.708886754509123 34.5 30.0 36.0 22.5 39.0 74-75 31.41912634353332 34.0 30.0 36.0 21.0 38.5 76-77 31.221440676970435 34.0 30.0 35.0 20.5 37.0 78-79 31.106283545498513 34.0 30.0 35.0 22.5 37.0 80-81 30.948697049268926 34.0 30.0 35.0 22.0 36.5 82-83 30.871764407552963 34.0 30.0 35.0 23.0 36.0 84-85 30.831727653141748 34.0 30.0 35.0 23.0 36.0 86-87 30.721824228930835 34.0 30.0 35.0 23.0 35.5 88-89 30.646030738984937 34.0 30.0 35.0 23.0 35.0 90-91 30.641931114361633 34.0 30.0 35.0 23.0 35.0 92-93 30.631738716669897 34.0 30.0 35.0 24.0 35.0 94-95 30.545856855569067 34.0 30.0 35.0 23.0 35.0 96-97 30.45182494365433 34.0 30.0 35.0 23.0 35.0 98-99 30.31184047175667 34.0 30.0 35.0 21.5 35.0 100-101 29.395492493445104 33.5 29.0 35.0 10.5 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.20136044615228954 1101 2 0.41106606198865947 1101 3 0.36305062106834995 1101 4 -0.01226737699202829 1101 5 -0.05009134694230255 1101 6 0.1391935471771646 1101 7 -0.24636958958038235 1101 8 0.06921339046335362 1101 9 0.805547375475399 1101 10-11 0.43184738223251884 1101 12-13 0.2625028332081101 1101 14-15 0.07378590815409325 1101 16-17 -0.004551480444220601 1101 18-19 0.07417416755264128 1101 20-21 0.9876611237259212 1101 22-23 0.6354849337503623 1101 24-25 0.3712562294614301 1101 26-27 1.140518769184439 1101 28-29 1.2212308726095458 1101 30-31 1.0939865702245442 1101 32-33 0.9299209440044365 1101 34-35 0.8448926681710702 1101 36-37 1.047729085878494 1101 38-39 0.9719902547234582 1101 40-41 0.9741849755332268 1101 42-43 0.7997268349730149 1101 44-45 1.0271557811748693 1101 46-47 0.8923151959531133 1101 48-49 0.8547852477219458 1101 50-51 0.5617914374166375 1101 52-53 0.43508349245417577 1101 54-55 0.6497724714534634 1101 56-57 0.9009713334248417 1101 58-59 0.7818145509473631 1101 60-61 0.8996041126096372 1101 62-63 0.835698207774282 1101 64-65 0.824304981738198 1101 66-67 1.4622767425354866 1101 68-69 1.5123218550441102 1101 70-71 2.0199706621163713 1101 72-73 1.4430566786850392 1101 74-75 1.1324419941774266 1101 76-77 1.3081378238465788 1101 78-79 1.5101792476943743 1101 80-81 2.25840846818479 1101 82-83 1.978388961733625 1101 84-85 1.9366544376007369 1101 86-87 1.8012521163621358 1101 88-89 1.8102364236041666 1101 90-91 1.683521668222884 1101 92-93 1.5357662373258236 1101 94-95 1.557349917553136 1101 96-97 1.692112796327347 1101 98-99 1.7205535011304747 1101 100-101 1.7636903134571043 1102 1 1.2931044480339757 1102 2 2.4594009328684905 1102 3 1.4096426179219215 1102 4 2.2038864046493742 1102 5 2.108439047797461 1102 6 2.55391967592427 1102 7 2.275472535723395 1102 8 2.6321293112512762 1102 9 2.1887342322001615 1102 10-11 2.9729084531939023 1102 12-13 3.0853379532765146 1102 14-15 3.5598836922555677 1102 16-17 3.294926428531099 1102 18-19 3.3449601092685413 1102 20-21 3.4092262978639027 1102 22-23 3.5866161149041815 1102 24-25 3.6075358682006566 1102 26-27 4.394305134087119 1102 28-29 4.368078131101477 1102 30-31 4.357106565366088 1102 32-33 4.287810581905006 1102 34-35 4.210684138306519 1102 36-37 4.529012662471935 1102 38-39 4.749523181836032 1102 40-41 4.6178423311267665 1102 42-43 4.6576250522866545 1102 44-45 4.718089655107626 1102 46-47 4.843026467855918 1102 48-49 4.619475082659829 1102 50-51 4.599201245131745 1102 52-53 4.551977706363047 1102 54-55 4.608254570845478 1102 56-57 4.702190863253726 1102 58-59 4.701102131909554 1102 60-61 4.5023195450458005 1102 62-63 4.341049929970961 1102 64-65 4.354198934408757 1102 66-67 4.373884592023664 1102 68-69 4.237120985920644 1102 70-71 4.121842034542915 1102 72-73 3.954534391008643 1102 74-75 4.044102573316735 1102 76-77 3.8294057068619445 1102 78-79 3.6554986958742433 1102 80-81 3.4577095834120968 1102 82-83 3.249399992545282 1102 84-85 3.0622305355487143 1102 86-87 2.951936493250926 1102 88-89 2.935494408483194 1102 90-91 2.8275828346181946 1102 92-93 2.625096821391054 1102 94-95 2.5763677771920115 1102 96-97 2.6239854122867925 1102 98-99 2.6049078328182986 1102 100-101 2.633669790344257 1103 1 1.2154239776675873 1103 2 2.298516687839701 1103 3 1.3345101204076748 1103 4 2.190325729625897 1103 5 1.9444331745857824 1103 6 2.349860681506094 1103 7 2.1645045659314306 1103 8 2.5953947164269735 1103 9 2.0426237203319673 1103 10-11 2.8918907820968585 1103 12-13 3.056875062132818 1103 14-15 3.3026406697924813 1103 16-17 3.1709861547772036 1103 18-19 3.2254037463844725 1103 20-21 3.3490759633349043 1103 22-23 3.5039455488270264 1103 24-25 3.4302460789729494 1103 26-27 4.31111641938314 1103 28-29 4.4035052633635345 1103 30-31 4.363523312452934 1103 32-33 4.311950920752352 1103 34-35 4.294171470471888 1103 36-37 4.351993177983779 1103 38-39 4.405078266947179 1103 40-41 4.312482483316678 1103 42-43 4.497991041978068 1103 44-45 4.44904711218804 1103 46-47 4.557840424014056 1103 48-49 4.336410857875055 1103 50-51 4.156877959974793 1103 52-53 4.237866990023647 1103 54-55 4.25589491631921 1103 56-57 4.346390309425907 1103 58-59 4.3667846518071975 1103 60-61 4.170016915267254 1103 62-63 4.104039488342405 1103 64-65 4.0708315311466166 1103 66-67 3.9612363955784673 1103 68-69 3.8649088639105464 1103 70-71 3.6804599537592324 1103 72-73 3.5182320772310547 1103 74-75 3.6098910981103245 1103 76-77 3.4088199656994256 1103 78-79 3.255264208018673 1103 80-81 3.135015981622523 1103 82-83 2.9428630583845 1103 84-85 2.8436813654729036 1103 86-87 2.739951571797647 1103 88-89 2.746763870037185 1103 90-91 2.595785203971328 1103 92-93 2.3143832874453096 1103 94-95 2.3160613870709312 1103 96-97 2.3906175873458118 1103 98-99 2.5131775399786953 1103 100-101 2.6180719762584985 1104 1 -0.2074509328414571 1104 2 -0.22757488874102805 1104 3 -0.16578878960975274 1104 4 -0.6310306007749915 1104 5 -0.5207295999456605 1104 6 -1.1244260415787295 1104 7 -0.8389185132253729 1104 8 -0.3830345656011076 1104 9 0.16806300527810691 1104 10-11 -0.5601624437780615 1104 12-13 -1.1356395952031662 1104 14-15 -1.352060538297735 1104 16-17 -1.2557516304505398 1104 18-19 -1.1003233659973404 1104 20-21 -0.5149693086563403 1104 22-23 -1.2212114854954095 1104 24-25 -1.3139611851976056 1104 26-27 -1.28635608595701 1104 28-29 -0.9698262441854908 1104 30-31 -1.395514231035989 1104 32-33 -1.312118226743138 1104 34-35 -1.2184051729515488 1104 36-37 -1.163683480234198 1104 38-39 -0.980414820026045 1104 40-41 -1.1872837434745023 1104 42-43 -1.0134201543067078 1104 44-45 -1.03406256256838 1104 46-47 -1.3278576196793814 1104 48-49 -1.5000756830931117 1104 50-51 -1.6141614956146952 1104 52-53 -1.584673125281384 1104 54-55 -1.5507924893389813 1104 56-57 -1.3823482725399998 1104 58-59 -1.1269730040988861 1104 60-61 -0.8908507904980176 1104 62-63 -1.1299996846767613 1104 64-65 -1.1636865550407052 1104 66-67 -0.7707409110593417 1104 68-69 -0.6869986899523113 1104 70-71 -0.9151205204367372 1104 72-73 -0.8993786957488297 1104 74-75 -0.8318202940555324 1104 76-77 -0.7036577159523425 1104 78-79 -0.8717394703683539 1104 80-81 -0.7959300765748836 1104 82-83 -0.939233945464391 1104 84-85 -0.875207749566961 1104 86-87 -0.8343295211935597 1104 88-89 -0.7645563478421096 1104 90-91 -0.7337847137103068 1104 92-93 -0.4670254418993949 1104 94-95 -0.3799179979061691 1104 96-97 -0.16965886978723788 1104 98-99 -0.1685353298647314 1104 100-101 9.56438390083747E-4 1105 1 -0.812691773047451 1105 2 -1.594405156551833 1105 3 -0.8922351610384034 1105 4 -1.6186280904179284 1105 5 -1.384861222015516 1105 6 -2.0702647984373463 1105 7 -1.5220624543464076 1105 8 -1.814266379527968 1105 9 -1.576931525270922 1105 10-11 -2.279377503224392 1105 12-13 -2.331362877064059 1105 14-15 -2.3571864387549155 1105 16-17 -2.711785788846946 1105 18-19 -2.2835920002918897 1105 20-21 -2.2659117432300917 1105 22-23 -2.556623236627658 1105 24-25 -2.638447514742044 1105 26-27 -3.515990855885221 1105 28-29 -3.377574309260659 1105 30-31 -3.0193992778068335 1105 32-33 -2.8978405213671365 1105 34-35 -3.1819118027032225 1105 36-37 -3.5176464632120954 1105 38-39 -3.307316014265499 1105 40-41 -3.2496379624848757 1105 42-43 -3.22679683751587 1105 44-45 -3.3284711739701365 1105 46-47 -3.4796031655163127 1105 48-49 -3.6814796687547826 1105 50-51 -3.5570906790111927 1105 52-53 -3.438196411186375 1105 54-55 -3.023749830793296 1105 56-57 -2.92608633599356 1105 58-59 -2.989249790432673 1105 60-61 -3.0071385736199154 1105 62-63 -3.190332942546604 1105 64-65 -3.30008780300329 1105 66-67 -2.857233270281938 1105 68-69 -2.8067296104099597 1105 70-71 -2.602176635536665 1105 72-73 -2.5000019237279965 1105 74-75 -2.3233804098437503 1105 76-77 -2.591056905783727 1105 78-79 -2.470135765494298 1105 80-81 -2.61383036644191 1105 82-83 -2.7331197970910104 1105 84-85 -2.648144949802788 1105 86-87 -2.396702870396691 1105 88-89 -2.486902117849219 1105 90-91 -2.596250423087593 1105 92-93 -2.5257635588778484 1105 94-95 -2.398524836167031 1105 96-97 -2.2805973355893947 1105 98-99 -2.249151548065491 1105 100-101 -2.196158292413415 1106 1 0.9617064168081981 1106 2 1.9406755139530993 1106 3 1.0319646387806074 1106 4 1.7552899894665899 1106 5 1.554543411883735 1106 6 1.9239334672464778 1106 7 1.8423287136305788 1106 8 2.381097017898874 1106 9 1.9190564071000722 1106 10-11 2.568345387356622 1106 12-13 2.6621832122090936 1106 14-15 2.5679337070082013 1106 16-17 2.435978327393194 1106 18-19 2.6821050073335044 1106 20-21 3.0096290056306216 1106 22-23 2.8694266491231986 1106 24-25 2.895740045787022 1106 26-27 3.7469391665996596 1106 28-29 3.9043107982065237 1106 30-31 3.7541049910763817 1106 32-33 3.8764631873877704 1106 34-35 3.931641707188575 1106 36-37 4.090174584987459 1106 38-39 4.041269844252092 1106 40-41 3.9348729491361 1106 42-43 4.138046934131829 1106 44-45 4.08394268298764 1106 46-47 3.9628971774676565 1106 48-49 3.8516317669051396 1106 50-51 3.8313870871798485 1106 52-53 3.760344403229901 1106 54-55 3.843870928285984 1106 56-57 3.9503621883643874 1106 58-59 3.9979532918949694 1106 60-61 3.8959738284775938 1106 62-63 3.7937037388283805 1106 64-65 3.6921090583048084 1106 66-67 3.7335603642059567 1106 68-69 3.6145068079318072 1106 70-71 3.513877176789272 1106 72-73 3.2526821032214173 1106 74-75 3.356453754873712 1106 76-77 3.258889399484847 1106 78-79 3.029321069551031 1106 80-81 2.8385860316384566 1106 82-83 2.781476992308729 1106 84-85 2.650489872632896 1106 86-87 2.5835144226184 1106 88-89 2.530601283267657 1106 90-91 2.372976379538148 1106 92-93 2.2792216370555423 1106 94-95 2.316094052889312 1106 96-97 2.332172413697773 1106 98-99 2.3607184153923555 1106 100-101 2.381091091060231 1107 1 -0.8757825325906197 1107 2 -2.3191672651243636 1107 3 -0.9385885943868288 1107 4 -1.845651782346735 1107 5 -2.1037215527690947 1107 6 -2.146295451058233 1107 7 -2.33580815746852 1107 8 -2.1273248431649527 1107 9 -0.37210566871172546 1107 10-11 -1.7459668989451487 1107 12-13 -2.0285127470322593 1107 14-15 -2.6699589150745098 1107 16-17 -3.10220871515617 1107 18-19 -2.825725652713132 1107 20-21 -1.8255645655008266 1107 22-23 -2.4813978068528755 1107 24-25 -2.878871941669132 1107 26-27 -3.167929066966046 1107 28-29 -2.78419716658496 1107 30-31 -3.0798031701920863 1107 32-33 -3.047694287243779 1107 34-35 -3.470773346999941 1107 36-37 -3.0517112498873047 1107 38-39 -3.3507611849509935 1107 40-41 -3.5581321555287104 1107 42-43 -3.8166006610901846 1107 44-45 -4.16701447853945 1107 46-47 -4.083607292432919 1107 48-49 -4.032380778907527 1107 50-51 -3.8915665479885497 1107 52-53 -4.255116899752757 1107 54-55 -4.3399735107171615 1107 56-57 -4.335585921257582 1107 58-59 -4.0745329483242045 1107 60-61 -3.840557811888889 1107 62-63 -3.7934547496558793 1107 64-65 -3.766384686981173 1107 66-67 -3.6780241567904284 1107 68-69 -3.5762411223237756 1107 70-71 -3.6570822858196266 1107 72-73 -3.5415781727313913 1107 74-75 -3.5089037535857166 1107 76-77 -3.5869990393491555 1107 78-79 -3.2796649499019637 1107 80-81 -3.0792923414377 1107 82-83 -3.0495281182562906 1107 84-85 -2.9097791965991853 1107 86-87 -2.7711891262594293 1107 88-89 -2.495192270359837 1107 90-91 -2.198598868295246 1107 92-93 -1.8985569438063408 1107 94-95 -1.1911318356576928 1107 96-97 -0.9184247366975882 1107 98-99 -0.32270302773994786 1107 100-101 0.1708022561254836 1108 1 -0.20194079852502256 1108 2 -0.8401357374859977 1108 3 -0.34647950061058097 1108 4 -1.1966935722116716 1108 5 -0.18753302175750264 1108 6 -1.2902923719859132 1108 7 -0.49280698184110605 1108 8 -1.2593666037150193 1108 9 0.1005230867506981 1108 10-11 -0.5933375066424347 1108 12-13 0.2534812757666245 1108 14-15 -1.7092159498854187 1108 16-17 -0.41710008839613977 1108 18-19 -1.3705600944518466 1108 20-21 -0.21606859427388514 1108 22-23 -0.9597245140145034 1108 24-25 -0.9548932363957832 1108 26-27 -1.1676728145589408 1108 28-29 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1.2142732078288816 2216 94-95 1.171244379455814 2216 96-97 1.1949342529121587 2216 98-99 1.145164827022711 2216 100-101 1.3104406733359184 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 48.0 11 544.0 12 2614.0 13 5570.0 14 7842.0 15 8263.0 16 8309.0 17 8588.0 18 9481.0 19 13649.0 20 15792.0 21 19309.0 22 26382.0 23 35300.0 24 44586.0 25 52917.0 26 59265.0 27 61786.0 28 63478.0 29 64739.0 30 67371.0 31 72638.0 32 83838.0 33 100728.0 34 131334.0 35 187499.0 36 294420.0 37 366926.0 38 209510.0 39 20018.0 40 4.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.7391028490525 32.70148885464133 13.572555961782864 23.986852334523295 2 26.198925976170557 24.798210515132926 27.285776601281015 21.717086907415503 3 23.857764149077614 26.32272801148257 21.75730927162822 28.062198567811595 4 27.219118559900686 22.017253229473237 23.46393191915988 27.2996962914662 5 24.502141233279875 25.770971260282714 23.235073538194627 26.491813968242777 6 23.75065353141944 20.68796542696407 24.749063516400458 30.81231752521603 7 27.833536001503855 22.137140753533966 22.9216232251849 27.10770001977728 8 27.547597647874333 20.53503417944847 24.544045569987098 27.373322602690102 9 22.974933765692096 23.444644175395105 25.151462637584277 28.428959421328525 10-11 26.701262221282313 20.76012312825664 23.718221728769283 28.820392921691763 12-13 26.083124301186437 20.878566519218232 23.765241723403964 29.273067456191367 14-15 25.993514618543255 20.850222347543603 23.86562121221022 29.290641821702923 16-17 26.152271352119794 22.233163366210615 23.886499950067265 27.72806533160233 18-19 24.24714159553699 20.473107794010815 25.68444565849532 29.595304951956873 20-21 24.40562167306383 24.08577562605207 23.997950752961213 27.510651947922888 22-23 24.068322259197203 22.483217451647292 26.02215969520322 27.426300593952284 24-25 23.96276975916755 20.7541018275382 27.743265444391575 27.539862968902675 26-27 23.886701611432642 21.040950974116967 28.4259312519046 26.646416162545787 28-29 24.037546481504325 21.09966574437963 28.257278920356306 26.60550885375974 30-31 23.968374953738785 21.07239855332131 28.310883182849768 26.648343310090134 32-33 23.914574876587814 21.168665934319847 28.36595605527456 26.55080313381778 34-35 24.055732767820604 21.2791054011557 28.18397080795086 26.481191023072842 36-37 24.111417561050114 21.14783615012718 28.028493969887624 26.712252318935082 38-39 24.28380789015581 21.219798036762246 27.779001619387216 26.71739245369473 40-41 25.071741595145365 21.535696032990852 25.926423621513766 27.466138750350016 42-43 25.147570821266257 21.506299357532107 25.90742959973526 27.438700221466377 44-45 25.117671998911163 21.772967214382863 25.81295757933007 27.296403207375906 46-47 24.91569453579185 22.157730340494453 25.592316716848927 27.334258406864762 48-49 24.946872801664824 22.21289650807078 25.28164391045552 27.558586779808874 50-51 24.80655276575757 22.45987681744206 25.371700419118337 27.361869997682035 52-53 25.02650840926047 22.657640590028727 25.109900976527697 27.20595002418311 54-55 25.08875913445005 22.433560682365293 25.14206968800598 27.335610495178674 56-57 24.981238498942844 22.76077262627237 25.331795861161467 26.926193013623323 58-59 25.007067738923233 22.9283569924109 25.148153270190786 26.91642199847508 60-61 25.114625005140134 23.00562771325685 25.00403867731115 26.875708604291866 62-63 25.091457907333425 23.086041660649432 24.990539681203167 26.831960750813977 64-65 25.14967582883449 23.32901562013523 24.924905078844773 26.59640347218551 66-67 25.310967759624507 23.148496086448926 24.798373721421946 26.742162432504617 68-69 25.259712746357195 23.310493347128872 24.648435310408164 26.78135859610577 70-71 25.26982128424504 23.31362615231202 24.558375241893124 26.85817732154982 72-73 25.313207691260832 23.220693443199163 24.484269611217123 26.981829254322882 74-75 25.30835913191446 23.232478993982618 24.693819306150342 26.765342567952583 76-77 25.364637577576275 23.321727232563006 24.498475704902344 26.81515948495837 78-79 25.49281653928923 23.23443714055772 24.580687387773725 26.692058932379325 80-81 25.417232081490226 23.294723578238727 24.681948042538775 26.606096297732268 82-83 25.57896303360132 23.65111783299645 24.54753329707497 26.222385836327263 84-85 25.669955373839553 23.659820007166815 24.480356852631846 26.189867766361786 86-87 25.585697576327593 23.748175623837504 24.512394007478168 26.153732792356738 88-89 25.682273077637635 23.742978265515006 24.485925444349824 26.08882321249753 90-91 25.742613517871778 23.860635682458373 24.415313322739603 25.981437476930246 92-93 25.65139256654913 24.06937450158831 24.47933877015125 25.79989416171131 94-95 25.945100369796343 24.219506487835492 24.30079412712104 25.53459901524712 96-97 25.910607028926037 24.21516738877525 24.281377333288624 25.592848249010093 98-99 26.006807593212773 24.30211889153623 24.38634486681722 25.304728648433777 100-101 26.115360289179083 24.441169444297582 24.090881498843714 25.352588767679617 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 407.0 1 369.5 2 298.0 3 193.0 4 100.0 5 72.5 6 54.5 7 42.0 8 34.0 9 30.5 10 32.5 11 29.5 12 32.5 13 39.0 14 48.0 15 82.5 16 422.5 17 448.0 18 173.5 19 192.0 20 221.5 21 286.5 22 413.0 23 603.0 24 831.0 25 1139.0 26 1595.0 27 2360.0 28 3278.0 29 4364.0 30 5874.0 31 7670.5 32 9657.5 33 12171.5 34 15014.0 35 18076.5 36 21708.0 37 25045.5 38 28519.0 39 32230.0 40 35476.0 41 38408.0 42 42420.0 43 47532.0 44 53029.5 45 58987.0 46 64112.5 47 69335.0 48 74599.0 49 77970.0 50 80133.5 51 82184.0 52 84707.5 53 87259.5 54 87730.5 55 86929.0 56 83383.0 57 77725.0 58 75528.5 59 73315.0 60 65343.5 61 56942.5 62 49759.5 63 41384.5 64 34866.5 65 30130.0 66 25373.0 67 21207.5 68 18450.5 69 16404.5 70 14998.5 71 14128.0 72 13231.0 73 11783.0 74 10107.0 75 8989.5 76 7829.0 77 6298.0 78 5079.0 79 4078.5 80 3155.5 81 2459.5 82 1839.5 83 1304.5 84 911.0 85 652.5 86 461.5 87 311.5 88 205.0 89 131.0 90 90.5 91 55.5 92 27.5 93 14.5 94 9.5 95 5.0 96 3.5 97 2.5 98 1.5 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0050911810952697055 2 0.0072940959922614046 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0022763453935580897 22-23 0.0036960016605082956 24-25 0.0 26-27 2.447683218879666E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.010084454861784223 46-47 0.0013462257703838164 48-49 4.4058297939833984E-4 50-51 3.671524828319499E-4 52-53 0.0 54-55 2.447683218879666E-5 56-57 4.895366437759332E-5 58-59 7.587817978526965E-4 60-61 0.0 62-63 2.447683218879666E-4 64-65 0.0 66-67 0.01436790049482364 68-69 0.0028637893660892096 70-71 3.4267565064315325E-4 72-73 6.853513012863065E-4 74-75 0.0 76-77 2.447683218879666E-5 78-79 0.0 80-81 0.0 82-83 4.895366437759332E-5 84-85 0.0 86-87 0.013168535717572604 88-89 0.003549140667375516 90-91 0.0014686099313277997 92-93 4.4058297939833984E-4 94-95 9.790732875518664E-5 96-97 1.7133782532157663E-4 98-99 0.0014196562669502063 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2042748.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.4258817251168 #Duplication Level Percentage of deduplicated Percentage of total 1 90.09048923457253 67.05064096330203 2 5.368141352890438 7.990573068278644 3 1.6765159273229686 3.7432852835164137 4 0.8794923694344071 2.6182798026267164 5 0.5520383202016218 2.0542969363529036 6 0.3537822916343004 1.5798335396169132 7 0.2389074840066543 1.244663010354714 8 0.16119704468278534 0.9597785745599481 9 0.12032657962127616 0.8059870604952853 >10 0.47711700408665697 6.36963836564061 >50 0.06679019470268008 3.3848614968836044 >100 0.0148677436391861 1.6148931485229265 >500 2.0067192272351416E-4 0.09946833067590571 >1k 6.689064090783762E-5 0.057351109578900265 >5k 6.689064090783762E-5 0.42644930959449623 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 8566 0.41933708905846434 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 9.790732875518664E-5 3 0.0 0.0 0.0 4.895366437759332E-5 9.790732875518664E-5 4 0.0 0.0 0.0 4.895366437759332E-5 9.790732875518664E-5 5 0.0 0.0 0.0 4.895366437759332E-5 9.790732875518664E-5 6 0.0 0.0 0.0 4.895366437759332E-5 1.4686099313277997E-4 7 0.0 0.0 0.0 9.790732875518664E-5 1.4686099313277997E-4 8 9.790732875518664E-5 0.0 0.0 9.790732875518664E-5 1.4686099313277997E-4 9 2.9372198626555993E-4 0.0 0.0 9.790732875518664E-5 1.4686099313277997E-4 10-11 4.895366437759332E-4 4.895366437759332E-5 0.0 9.790732875518664E-5 2.2029148969916995E-4 12-13 9.301196231742731E-4 4.895366437759332E-5 0.0 9.790732875518664E-5 3.4267565064315325E-4 14-15 0.0012727952738174264 4.895366437759332E-5 0.0 9.790732875518664E-5 4.6505981158713656E-4 16-17 0.002300822225746886 4.895366437759332E-5 0.0 9.790732875518664E-5 4.895366437759332E-4 18-19 0.004919843269948129 4.895366437759332E-5 0.0 9.790732875518664E-5 5.384903081535265E-4 20-21 0.008199738783246882 4.895366437759332E-5 0.0 9.790732875518664E-5 5.874439725311199E-4 22-23 0.014147609005124469 4.895366437759332E-5 0.0 9.790732875518664E-5 5.874439725311199E-4 24-25 0.020389201213267618 4.895366437759332E-5 0.0 9.790732875518664E-5 6.363976369087132E-4 26-27 0.023497758901244795 4.895366437759332E-5 0.0 9.790732875518664E-5 6.853513012863065E-4 28-29 0.025945442120124458 4.895366437759332E-5 0.0 9.790732875518664E-5 7.098281334751032E-4 30-31 0.027952542359605786 9.790732875518664E-5 0.0 9.790732875518664E-5 7.343049656638998E-4 32-33 0.029641443780632756 9.790732875518664E-5 0.0 1.4686099313277997E-4 8.811659587966798E-4 34-35 0.03140377569822611 9.790732875518664E-5 0.0 1.4686099313277997E-4 9.301196231742731E-4 36-37 0.03485500903684645 9.790732875518664E-5 0.0 1.4686099313277997E-4 9.545964553630698E-4 38-39 0.039358746159585026 9.790732875518664E-5 0.0 1.4686099313277997E-4 0.001003550119740663 40-41 0.04506184805957465 9.790732875518664E-5 0.0 2.2029148969916995E-4 0.0010280269519294598 42-43 0.05213565256213688 9.790732875518664E-5 0.0 2.447683218879666E-4 0.001076980616307053 44-45 0.059062596071566345 9.790732875518664E-5 0.0 2.9372198626555993E-4 0.001150411112873443 46-47 0.06968554124150408 9.790732875518664E-5 0.0 2.9372198626555993E-4 0.0012483184416286297 48-49 0.08393105757538374 9.790732875518664E-5 0.0 2.9372198626555993E-4 0.0012727952738174264 50-51 0.10197048289852689 9.790732875518664E-5 0.0 2.9372198626555993E-4 0.001370702602572613 52-53 0.1239017245396887 9.790732875518664E-5 0.0 2.9372198626555993E-4 0.0014196562669502063 54-55 0.14901495436539408 9.790732875518664E-5 0.0 3.4267565064315325E-4 0.0014930867635165963 56-57 0.1831356584365766 9.790732875518664E-5 0.0 3.4267565064315325E-4 0.001517563595705393 58-59 0.22019358237041475 9.790732875518664E-5 0.0 3.4267565064315325E-4 0.0015420404278941896 60-61 0.2674583453269811 9.790732875518664E-5 0.0 3.4267565064315325E-4 0.0016154709244605796 62-63 0.3217479591217321 1.4686099313277997E-4 0.0 3.4267565064315325E-4 0.0016399477566493763 64-65 0.3868563327439312 1.4686099313277997E-4 0.0 3.4267565064315325E-4 0.0017133782532157663 66-67 0.45754542410517596 1.4686099313277997E-4 0.0 3.4267565064315325E-4 0.001811285581970953 68-69 0.5420884024852797 1.4686099313277997E-4 0.0 3.4267565064315325E-4 0.0018357624141597496 70-71 0.6321876217722402 1.4686099313277997E-4 0.0 3.4267565064315325E-4 0.001958146575103733 72-73 0.7372911391909329 1.4686099313277997E-4 0.0 3.4267565064315325E-4 0.0020560539038589195 74-75 0.8558079606490865 1.4686099313277997E-4 0.0 3.4267565064315325E-4 0.0021294844004253093 76-77 0.9916054256325304 1.4686099313277997E-4 0.0 3.4267565064315325E-4 0.0022029148969916995 78-79 1.1451485939528516 1.4686099313277997E-4 0.0 3.4267565064315325E-4 0.0022029148969916995 80-81 1.3136715835727166 1.4686099313277997E-4 0.0 3.671524828319499E-4 0.0022029148969916995 82-83 1.504174768498121 1.4686099313277997E-4 0.0 4.1610614720954324E-4 0.002251868561369293 84-85 1.7158993669312124 1.4686099313277997E-4 0.0 4.405829793983399E-4 0.0023497758901244795 86-87 1.9444395490780066 1.4686099313277997E-4 0.0 4.6505981158713656E-4 0.0023987295545020728 88-89 2.201128088241917 1.4686099313277997E-4 0.0 4.895366437759332E-4 0.0024232063866908692 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 6425 0.0 93.89918 1 TCGACGG 6575 0.0 91.7525 2 ACGGCGC 7010 0.0 85.847176 5 TATCTCG 2535 0.0 85.447 2 CGACGGC 7140 0.0 84.41718 3 GACGGCG 7155 0.0 84.2402 4 ATCTCGA 2635 0.0 81.29491 3 CGGCGCG 8220 0.0 77.775116 6 ATATCTC 2875 0.0 75.34565 1 TCTCGAG 2925 0.0 71.12392 4 CTCGAGG 3045 0.0 68.00903 5 AGGGCGC 3390 0.0 62.06853 9 CGAGGGC 3445 0.0 60.80185 7 TCGAGGG 3540 0.0 58.901817 6 CGCGCCG 11185 0.0 57.964767 9 GAGGGCG 3630 0.0 57.441444 8 GGCGCGC 11695 0.0 55.43702 7 GCGCGCC 12320 0.0 52.817432 8 TCGCCGG 935 0.0 41.65932 2 TCCATAT 7570 0.0 39.751183 18-19 >>END_MODULE