FastQCFastQC Report
Tue 31 May 2016
SRR2048264_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048264_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2042748
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG13550.089.800141
TCGACGG14700.083.376722
CGACGGC19850.062.702263
ACGGCGC20900.062.4993745
GACGGCG21750.059.8414084
CGGCGCG25150.052.126716
CGCGCCG44800.037.3210539
ATCTCGA11050.033.981391
GGCGCGC51050.032.7518737
GCGCGCC52200.032.030338
TCTCGAG14400.026.721752
CTCGAGG17850.022.6215883
TCCATAT30100.022.01389918-19
ATCCATA29950.021.96555516-17
CCATATT30750.021.625818-19
GGGCGCG19500.021.1920938
GCCGGAT42800.020.75329612-13
GTTGACG3503.6379788E-1220.3704011
CGGATCC43850.020.03970514-15
CGCCGGA42550.019.98217210-11
Produced by FastQC (version 0.11.5)