##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048264_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2042748 Sequences flagged as poor quality 0 Sequence length 101 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.844428681364516 31.0 28.0 33.0 16.0 34.0 2 30.657078112425026 31.0 30.0 33.0 27.0 34.0 3 28.540722595249143 31.0 28.0 33.0 16.0 34.0 4 33.01366443633772 35.0 33.0 37.0 25.0 37.0 5 34.26950705618118 35.0 35.0 37.0 32.0 37.0 6 34.90061573919054 35.0 35.0 37.0 32.0 37.0 7 35.11385349538954 35.0 35.0 37.0 33.0 37.0 8 35.2724472132637 35.0 35.0 37.0 33.0 37.0 9 37.05106944175199 39.0 37.0 39.0 34.0 39.0 10-11 37.05169923064421 39.0 37.0 39.0 34.0 39.0 12-13 37.00463224049173 39.0 37.0 39.0 34.0 39.0 14-15 38.0145801146299 39.0 37.0 40.0 34.0 41.0 16-17 37.95598624989475 39.0 37.0 40.0 34.0 41.0 18-19 38.13068180705598 39.0 37.0 40.0 34.0 41.0 20-21 38.169295723212066 39.0 37.0 40.0 34.0 41.0 22-23 38.09331155874342 39.0 37.0 40.0 34.0 41.0 24-25 38.018274402912155 39.0 37.0 40.0 34.0 41.0 26-27 38.26761230460145 39.0 37.5 40.0 34.5 41.0 28-29 38.45495332757638 39.0 38.0 40.0 35.0 41.0 30-31 38.40828090395878 39.0 38.0 40.0 35.0 41.0 32-33 38.37814111187479 40.0 38.0 40.0 35.0 41.0 34-35 38.350510684626784 40.0 38.0 40.0 35.0 41.0 36-37 38.3217900592731 40.0 38.0 40.0 35.0 41.0 38-39 38.214397958044756 39.5 37.5 40.0 34.5 41.0 40-41 38.083644433870454 39.0 37.0 40.0 34.0 41.0 42-43 37.97706912453225 39.0 37.0 40.0 34.0 41.0 44-45 37.83375825114013 39.0 37.0 40.0 34.0 41.0 46-47 37.68080962507368 39.0 36.5 40.0 33.0 41.0 48-49 37.54475099229078 39.0 36.0 40.0 33.0 41.0 50-51 37.36050114845297 39.0 36.0 40.0 33.0 41.0 52-53 37.167051687237 38.0 35.0 40.0 33.0 41.0 54-55 36.93226795473548 38.0 35.0 40.0 33.0 41.0 56-57 36.64719289897726 38.0 35.0 40.0 32.5 40.5 58-59 36.34468103750438 37.5 35.0 39.5 32.0 40.0 60-61 35.970353416084606 37.0 34.5 39.0 31.5 40.0 62-63 35.65084019174171 36.0 34.0 39.0 31.0 40.0 64-65 35.320167979603944 36.0 34.0 38.5 31.0 40.0 66-67 34.96354983580941 35.0 34.0 38.0 31.0 40.0 68-69 34.60085629749729 35.0 33.5 37.0 30.5 39.0 70-71 34.23961521440726 35.0 33.0 36.5 30.0 39.0 72-73 33.891973459281324 35.0 33.0 36.0 30.0 38.5 74-75 33.54098278397531 34.0 33.0 35.5 30.0 37.5 76-77 33.213982830970835 34.0 33.0 35.0 29.0 37.0 78-79 32.89774876783627 34.0 32.0 35.0 29.0 36.0 80-81 32.53707407864308 34.0 32.0 35.0 29.0 36.0 82-83 32.33244457955656 34.0 32.0 35.0 29.0 35.0 84-85 32.06023577063837 34.0 31.0 35.0 27.5 35.0 86-87 31.851430034443798 34.0 31.0 34.5 27.0 35.0 88-89 31.589402608642867 33.5 31.0 34.0 27.0 35.0 90-91 31.345536258021056 33.0 31.0 34.0 27.0 35.0 92-93 31.07798881702491 33.0 30.0 34.0 26.0 35.0 94-95 30.773146761127656 33.0 30.0 34.0 25.0 35.0 96-97 30.41133194109112 33.0 30.0 34.0 24.5 35.0 98-99 29.911428624578264 32.5 29.5 34.0 24.0 35.0 100-101 27.795873255046633 30.5 26.5 33.0 19.5 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.8962724234923627 1101 2 1.4790462945817104 1101 3 2.621958722560901 1101 4 1.7498868668324192 1101 5 0.5737880505610562 1101 6 0.6570350325594134 1101 7 0.18714813550872123 1101 8 0.4505307935352221 1101 9 0.6560652971540293 1101 10-11 0.571671237214872 1101 12-13 0.38067812997502415 1101 14-15 0.3607931197136267 1101 16-17 0.1592451292726409 1101 18-19 0.2847050729828524 1101 20-21 0.2141006929251148 1101 22-23 0.17967444786982867 1101 24-25 0.1433546386388116 1101 26-27 0.15485514315547988 1101 28-29 0.12973844907752863 1101 30-31 0.14008148123476616 1101 32-33 0.15874846511662355 1101 34-35 0.11185256071399152 1101 36-37 0.054644513095425395 1101 38-39 0.07825555407951867 1101 40-41 0.11891278029489882 1101 42-43 0.1802013554067443 1101 44-45 0.17391108523792553 1101 46-47 0.16697610737571011 1101 48-49 0.14549749853910043 1101 50-51 0.019349448865241925 1101 52-53 0.09126350743486 1101 54-55 0.04138314157304279 1101 56-57 0.08334735188732623 1101 58-59 0.0852479499753045 1101 60-61 0.038758486412149296 1101 62-63 -0.009239489029397419 1101 64-65 0.04189308547655202 1101 66-67 -0.026116341337150573 1101 68-69 -0.00104197765756453 1101 70-71 0.02837220155619491 1101 72-73 0.0373915273894454 1101 74-75 0.08400649585479414 1101 76-77 -0.05195784568829964 1101 78-79 0.00786592262872432 1101 80-81 0.011940556832662708 1101 82-83 0.1928436257383197 1101 84-85 0.1271443675682562 1101 86-87 0.07735112986398462 1101 88-89 0.09372087005840868 1101 90-91 0.09726793358743535 1101 92-93 0.10786653690686876 1101 94-95 0.14393489472371712 1101 96-97 0.0709050997699805 1101 98-99 0.09752983997632114 1101 100-101 0.16916725789175402 1102 1 0.2723100263850675 1102 2 0.6906992582869371 1102 3 -1.0189939971612425 1102 4 1.0777018060005261 1102 5 1.0381563985034958 1102 6 0.7652362766707057 1102 7 0.893800650963712 1102 8 0.6291491609982316 1102 9 0.7096638789481275 1102 10-11 0.49728917185130683 1102 12-13 0.3199492847351024 1102 14-15 0.14608974087084903 1102 16-17 0.01254680553571319 1102 18-19 -0.023490580050257392 1102 20-21 7.148805751953091E-4 1102 22-23 -0.015153901210105403 1102 24-25 -0.0889130159135334 1102 26-27 -0.08805908102940663 1102 28-29 -0.13879428813119432 1102 30-31 -0.11247446087636348 1102 32-33 -0.037111148694116025 1102 34-35 -0.05198540598438939 1102 36-37 -0.10244116632178901 1102 38-39 0.02705409885682286 1102 40-41 -0.07947322967071102 1102 42-43 -0.023166594412366237 1102 44-45 -0.077460897869301 1102 46-47 -0.06889451507516497 1102 48-49 -0.054708696568319226 1102 50-51 -0.10001766036418047 1102 52-53 -0.1937688870793366 1102 54-55 -0.035987065884881986 1102 56-57 -0.07821851631553756 1102 58-59 -0.03307160327826608 1102 60-61 0.013854571018661943 1102 62-63 -0.04372053575634993 1102 64-65 -0.10167943996526674 1102 66-67 -0.19407714980705038 1102 68-69 -0.12354616373635707 1102 70-71 -0.20760359100323456 1102 72-73 -0.1302621573720728 1102 74-75 -0.07090868845317289 1102 76-77 -0.02699425999490046 1102 78-79 0.025502111171448405 1102 80-81 -0.1288591794226832 1102 82-83 -0.051704639244078976 1102 84-85 -0.03150305422170874 1102 86-87 -0.05469480951908423 1102 88-89 -0.10011515904218626 1102 90-91 -0.08209105893883617 1102 92-93 -0.09597810052617461 1102 94-95 -0.16107403654168806 1102 96-97 -0.22681663226892468 1102 98-99 -0.17022670134415563 1102 100-101 -0.24043349092712774 1103 1 0.0761369425410301 1103 2 0.5203241414001454 1103 3 1.1024581132896962 1103 4 1.9682436185551637 1103 5 1.2945541700427228 1103 6 0.8419045457169503 1103 7 0.7405427688826691 1103 8 0.5311466966726925 1103 9 0.5371065310567218 1103 10-11 0.40094528226163106 1103 12-13 0.17602126204224078 1103 14-15 0.11083429213486085 1103 16-17 0.04422641774264946 1103 18-19 -0.04515506173209616 1103 20-21 4.09980959709344E-4 1103 22-23 0.0624948141582351 1103 24-25 -0.036154195037710224 1103 26-27 -0.016288482577635932 1103 28-29 -0.02170500460828606 1103 30-31 0.021108403059578507 1103 32-33 0.07643577443590033 1103 34-35 0.040142297643889435 1103 36-37 -0.03390088592429663 1103 38-39 -0.05383474505298125 1103 40-41 -0.018523684662177686 1103 42-43 -0.06994695602003986 1103 44-45 -0.0399819108141557 1103 46-47 -0.03393200404482144 1103 48-49 -0.011787298708142657 1103 50-51 -0.04115637221967461 1103 52-53 -0.0333305889338007 1103 54-55 -0.08655332892745093 1103 56-57 -0.06697905419873251 1103 58-59 -0.06918400667724 1103 60-61 -0.149125331768019 1103 62-63 -0.026018888506705196 1103 64-65 -0.16367199466604632 1103 66-67 -0.18173297519690124 1103 68-69 -0.10511759380763408 1103 70-71 -0.1234728974138406 1103 72-73 -0.13154320046437817 1103 74-75 -0.11175077936746902 1103 76-77 -0.042195093576509635 1103 78-79 -0.04464992900542342 1103 80-81 -0.19737811770765745 1103 82-83 -0.21746409401507094 1103 84-85 -0.15383871091937706 1103 86-87 -0.15306697430979455 1103 88-89 -0.3058014895396006 1103 90-91 -0.29822839818669067 1103 92-93 -0.3748621111359576 1103 94-95 -0.3482820562191158 1103 96-97 -0.48333152725626505 1103 98-99 -0.3966859639844955 1103 100-101 -0.6674871830351456 1104 1 -0.20157586123315596 1104 2 -0.3970697752925858 1104 3 0.6154540388424543 1104 4 -0.2141193761360327 1104 5 -0.09563085401482851 1104 6 0.18039065794549458 1104 7 0.38256418916880364 1104 8 0.31581497493145605 1104 9 0.17269898829420782 1104 10-11 0.13488353096862227 1104 12-13 0.10781778406523301 1104 14-15 0.09022211092115384 1104 16-17 0.06901130642930298 1104 18-19 0.10093117069983037 1104 20-21 0.03970497069145296 1104 22-23 0.05514110379333914 1104 24-25 0.04536196785989688 1104 26-27 0.042073520719789315 1104 28-29 0.02041537744950972 1104 30-31 0.0398387654440171 1104 32-33 0.06595727331245627 1104 34-35 0.12258174261256727 1104 36-37 0.07596222110235828 1104 38-39 0.07409869800500957 1104 40-41 0.1179142634475383 1104 42-43 0.06792058297369152 1104 44-45 0.18283437212699738 1104 46-47 0.1271981226926684 1104 48-49 0.19620132459543527 1104 50-51 0.18471823263034537 1104 52-53 0.13248654633603252 1104 54-55 0.1508152363595272 1104 56-57 0.2492460319710048 1104 58-59 0.2655702148717367 1104 60-61 0.14709875803499983 1104 62-63 0.2333430069841924 1104 64-65 0.27071294957560355 1104 66-67 0.2416315048089004 1104 68-69 0.17475995432034352 1104 70-71 0.2275498526045041 1104 72-73 0.2004311845633353 1104 74-75 0.13887647877515974 1104 76-77 0.1392869779498298 1104 78-79 0.16473751503331613 1104 80-81 0.16519618132348057 1104 82-83 0.14627263286163839 1104 84-85 0.1258618533509832 1104 86-87 0.13125785935823586 1104 88-89 0.13120424551884824 1104 90-91 0.17883705138516603 1104 92-93 0.1671661824821271 1104 94-95 0.1912917297098069 1104 96-97 0.056780980485118704 1104 98-99 0.04194255231533717 1104 100-101 -0.2218328604819284 1105 1 -1.0467616540616937 1105 2 -0.6822864777548503 1105 3 -1.3584735899533023 1105 4 -0.11801999270313956 1105 5 -0.1971683664395414 1105 6 -0.2954668405548162 1105 7 -0.42783557833492836 1105 8 -0.20432089106758866 1105 9 -0.35106626183950596 1105 10-11 -0.09324718950418287 1105 12-13 -0.1846680942841843 1105 14-15 -0.24310910784856077 1105 16-17 -0.17740430999308643 1105 18-19 -0.16164361051221476 1105 20-21 -0.14381314907029719 1105 22-23 -0.10261905394254 1105 24-25 0.04559202577382848 1105 26-27 -0.014824331187547557 1105 28-29 0.06660418709953575 1105 30-31 0.0509380438095306 1105 32-33 0.1285428387989711 1105 34-35 0.1133793269350889 1105 36-37 0.11218905721429451 1105 38-39 0.11406702316357098 1105 40-41 0.13739732400878069 1105 42-43 0.16065414280321733 1105 44-45 0.16159728104230453 1105 46-47 0.16918056970005324 1105 48-49 0.09331062361363252 1105 50-51 0.03812634089811695 1105 52-53 0.12315249907315717 1105 54-55 0.1556259346769835 1105 56-57 0.1751938487699718 1105 58-59 0.14110095382053345 1105 60-61 0.18374427774385538 1105 62-63 0.17354083498374706 1105 64-65 0.31670223032396905 1105 66-67 0.15277225505342784 1105 68-69 0.11398006096570867 1105 70-71 0.15221673945010394 1105 72-73 0.18991275039241629 1105 74-75 0.2616716992798729 1105 76-77 0.29917078532207597 1105 78-79 0.29116786864253896 1105 80-81 0.150119012306547 1105 82-83 0.13049468424473787 1105 84-85 0.25688702154800325 1105 86-87 0.1573116950907405 1105 88-89 0.18576236581980865 1105 90-91 0.08050567997038982 1105 92-93 0.13737541723080327 1105 94-95 0.15511425544239543 1105 96-97 0.18417012349609152 1105 98-99 0.27440214796055074 1105 100-101 0.06296119357662278 1106 1 0.21911235356573044 1106 2 0.4175627444656449 1106 3 1.2504733242385306 1106 4 1.0242366458106815 1106 5 0.9881375921550273 1106 6 0.7095644470171365 1106 7 0.6653991558372923 1106 8 0.48913524516901674 1106 9 0.4885099654179541 1106 10-11 0.408494628385192 1106 12-13 0.24565682473442507 1106 14-15 0.004403667037038872 1106 16-17 0.0134438640143415 1106 18-19 -0.1019335823368479 1106 20-21 -0.023011289725481276 1106 22-23 -0.09518248570156373 1106 24-25 -0.0015679656025753275 1106 26-27 -0.12514879064143258 1106 28-29 -0.07572037237726903 1106 30-31 -0.04287330892599073 1106 32-33 -0.028555374913501907 1106 34-35 -0.039446016167154596 1106 36-37 -0.07227995291707145 1106 38-39 -0.12202320290847979 1106 40-41 -0.11575329630222342 1106 42-43 -0.13130347451588165 1106 44-45 -0.11761802570701718 1106 46-47 -0.18107810441516392 1106 48-49 -0.1111695242040085 1106 50-51 -0.04109842747788406 1106 52-53 -0.18474728337701407 1106 54-55 -0.16580417667960745 1106 56-57 -0.17746287462816213 1106 58-59 -0.25046346532682406 1106 60-61 -0.22632262271510228 1106 62-63 -0.1965139951428867 1106 64-65 -0.4314027072259137 1106 66-67 -0.41894140097995347 1106 68-69 -0.2925938096708691 1106 70-71 -0.2738180076980612 1106 72-73 -0.2119276035805555 1106 74-75 -0.1339506088028628 1106 76-77 -0.4145343809288491 1106 78-79 -0.3187879252859389 1106 80-81 -0.27040771645091866 1106 82-83 -0.26157248519474763 1106 84-85 -0.2526511767284987 1106 86-87 -0.41541678798748194 1106 88-89 -0.2636411799923728 1106 90-91 -0.28427679385300664 1106 92-93 -0.25036501790940946 1106 94-95 -0.2835988011293473 1106 96-97 -0.33303361438710866 1106 98-99 -0.2772336659184411 1106 100-101 -0.37534568887820896 1107 1 -2.299502021508637 1107 2 -0.6166142205254594 1107 3 -1.8376030402095616 1107 4 -3.6841280815553716 1107 5 -1.9871523294016846 1107 6 -0.4706852891943214 1107 7 -0.3382667428187389 1107 8 0.025283863585734423 1107 9 0.2959181704463063 1107 10-11 0.4080394272027874 1107 12-13 0.20811428176369162 1107 14-15 0.06066118365841788 1107 16-17 0.02672223689383202 1107 18-19 0.06878746262963631 1107 20-21 -0.10027935493854301 1107 22-23 -0.04316653153439631 1107 24-25 -0.14938183955246132 1107 26-27 -0.07055695240158144 1107 28-29 -0.058119014148978465 1107 30-31 0.06213493661713443 1107 32-33 0.12745162863547677 1107 34-35 0.1200517481450376 1107 36-37 0.12850251323293094 1107 38-39 0.0810969263454524 1107 40-41 0.14212992477961706 1107 42-43 0.10176946289370647 1107 44-45 0.14500055621689967 1107 46-47 0.10072260026655755 1107 48-49 0.10562645539306459 1107 50-51 0.030419145805183234 1107 52-53 0.07951170436333399 1107 54-55 -0.0013025919816058718 1107 56-57 0.05141407944756082 1107 58-59 0.10812183154714461 1107 60-61 0.06316256431662737 1107 62-63 0.08976329701484786 1107 64-65 0.06758476761214638 1107 66-67 -0.01540840454859449 1107 68-69 0.01713475117946217 1107 70-71 0.05898922359900638 1107 72-73 0.012025920467216622 1107 74-75 -0.025476530882720283 1107 76-77 -0.016071729748261987 1107 78-79 -0.039155203509345426 1107 80-81 -0.0072482819663548526 1107 82-83 0.03186559652905885 1107 84-85 0.015537104864911555 1107 86-87 0.011070286128003204 1107 88-89 0.08483548418637454 1107 90-91 0.12134118919962589 1107 92-93 0.02609122761012017 1107 94-95 -0.144962015091604 1107 96-97 -0.07016122510700384 1107 98-99 -0.1046994864950257 1107 100-101 -0.0036031700983336634 1108 1 -1.5504954418363788 1108 2 -0.37355426352734256 1108 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2214 34-35 0.06202369553093945 2214 36-37 0.05789674051162308 2214 38-39 0.14033953665972376 2214 40-41 0.22137392536661338 2214 42-43 0.2513628081215984 2214 44-45 0.188060005823381 2214 46-47 0.22061122397379762 2214 48-49 0.10918362263515036 2214 50-51 0.02675152911881895 2214 52-53 0.18636652127214148 2214 54-55 0.11849102975607906 2214 56-57 0.17752610453425888 2214 58-59 0.18193179671308712 2214 60-61 0.24083208104238452 2214 62-63 0.23121891255809857 2214 64-65 0.32848064390876885 2214 66-67 0.1884507608243453 2214 68-69 0.012286561237317528 2214 70-71 0.02215008587705114 2214 72-73 0.08802479280156916 2214 74-75 0.13082951448651414 2214 76-77 0.06771266984701896 2214 78-79 0.17309425775997767 2214 80-81 0.2837972031229654 2214 82-83 0.20868125408944138 2214 84-85 0.23600076081875443 2214 86-87 0.23846290686933003 2214 88-89 0.22594082824025108 2214 90-91 0.178063544012673 2214 92-93 0.16109945339041332 2214 94-95 0.10240573521541663 2214 96-97 -0.05400200798502297 2214 98-99 0.12662142706863122 2214 100-101 0.519191895978242 2215 1 -1.2948117471969667 2215 2 0.06354038982796695 2215 3 -0.46603098030887935 2215 4 -4.0328314898457975 2215 5 -1.3672475845192835 2215 6 -0.3195175713059655 2215 7 0.16511456216171894 2215 8 0.09600341280454217 2215 9 0.21030437581797656 2215 10-11 0.19844806076115162 2215 12-13 0.11929950992451666 2215 14-15 -0.04589946557252489 2215 16-17 -0.026800622199772306 2215 18-19 -0.13043864131243055 2215 20-21 -0.09732959406398578 2215 22-23 -0.2108460285537319 2215 24-25 -0.11929123599923486 2215 26-27 -0.042742907289692766 2215 28-29 0.060640442306166165 2215 30-31 0.004042074824987196 2215 32-33 0.016763432872160422 2215 34-35 0.058127239848843715 2215 36-37 -0.005940122623812272 2215 38-39 -0.008898578204323826 2215 40-41 -0.03201285721907965 2215 42-43 -0.01919940698960687 2215 44-45 0.07981023554889077 2215 46-47 0.03637680934290444 2215 48-49 0.11768651218675785 2215 50-51 0.0961529985844436 2215 52-53 -0.1670502001527936 2215 54-55 -0.10706753696411653 2215 56-57 -0.13177878301365098 2215 58-59 -0.09092378532516676 2215 60-61 0.04183872590942883 2215 62-63 -0.05428641227830866 2215 64-65 0.015241844728393517 2215 66-67 0.06356329068218258 2215 68-69 -0.006855373485642247 2215 70-71 -0.13735808768526425 2215 72-73 -0.09249305839333033 2215 74-75 0.04636401349049635 2215 76-77 0.05640434257272631 2215 78-79 -0.10458160699942454 2215 80-81 0.09468923796607243 2215 82-83 -0.08006394565187236 2215 84-85 -0.07921788991163936 2215 86-87 0.09906585694663761 2215 88-89 0.046600182572245075 2215 90-91 0.046519023921899105 2215 92-93 0.031140006909005535 2215 94-95 0.18686553357538926 2215 96-97 0.1764678244222786 2215 98-99 0.1807299819299324 2215 100-101 0.13358755264326305 2216 1 1.5271864210093398 2216 2 0.6345662360044351 2216 3 0.684202193663122 2216 4 2.083292358651306 2216 5 1.4735635685689559 2216 6 1.0007968617172267 2216 7 0.8572778779275083 2216 8 0.6831337381462745 2216 9 0.6642582752563229 2216 10-11 0.4932213091241877 2216 12-13 0.40203438392638446 2216 14-15 0.29698581609218166 2216 16-17 0.32483921918703373 2216 18-19 0.024326393755565334 2216 20-21 0.04134633509411145 2216 22-23 0.07225896748268923 2216 24-25 0.17020098392114846 2216 26-27 0.09584926914911307 2216 28-29 0.04008285966860825 2216 30-31 0.055232105428920875 2216 32-33 0.05404411059121372 2216 34-35 0.026184524962985734 2216 36-37 -0.07292401504089696 2216 38-39 -0.010381976106948798 2216 40-41 -0.025276219124044985 2216 42-43 -0.01275452127400456 2216 44-45 -0.05780353260282567 2216 46-47 0.04993228637792413 2216 48-49 0.05819938532403057 2216 50-51 -0.05872008573577858 2216 52-53 -0.0692695550715996 2216 54-55 -0.007834095002806407 2216 56-57 -0.06638808361006454 2216 58-59 0.06540107745762214 2216 60-61 0.09702895515327725 2216 62-63 0.023464384439442654 2216 64-65 -0.003128128520202722 2216 66-67 0.02799903449992769 2216 68-69 0.1018568200664518 2216 70-71 0.06601936317293422 2216 72-73 0.0366007740408989 2216 74-75 0.01069330842644689 2216 76-77 0.028875837308042662 2216 78-79 -0.06830892017242007 2216 80-81 0.016096646400882264 2216 82-83 0.14457238956579488 2216 84-85 0.19507357517705515 2216 86-87 0.17821261314610837 2216 88-89 0.2399546589004906 2216 90-91 0.29388658429684256 2216 92-93 0.1836000235935913 2216 94-95 0.17848870977141829 2216 96-97 0.3092018206452103 2216 98-99 0.34609941095558483 2216 100-101 0.5260172476427236 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 7.0 13 59.0 14 174.0 15 396.0 16 718.0 17 1152.0 18 2061.0 19 3228.0 20 3338.0 21 3430.0 22 3640.0 23 4224.0 24 5080.0 25 6640.0 26 8975.0 27 12090.0 28 16844.0 29 22910.0 30 33087.0 31 48390.0 32 74765.0 33 124214.0 34 226657.0 35 431328.0 36 635429.0 37 350753.0 38 23135.0 39 22.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 30.075358843472443 31.774091956792827 12.803901458491904 25.34664774124283 2 27.155496310598053 26.73209503104404 24.72856350505179 21.38384515330612 3 25.89676915853395 24.432415441883876 21.39570157911667 28.275113820465503 4 26.197964041251808 25.305016855357245 22.21392155576465 26.283097547626298 5 25.608445094549108 26.324049760420763 23.71149059991737 24.356014545112757 6 25.23762108688884 24.381715218910998 24.227364315128444 26.153299379071722 7 26.66099783233174 24.767812769857077 23.287796634729297 25.283392763081885 8 25.668119611425393 23.880968185992593 24.820291098069855 25.63062110451216 9 24.34460834131278 23.3642622584871 25.652209670502675 26.638919729697445 10-11 25.748379144172457 24.474115260423705 24.199118050782573 25.57838754462127 12-13 25.769869802834222 24.484836112922398 24.200635614378278 25.544658469865105 14-15 25.495974050641586 24.56793495820336 24.36396951557412 25.572121475580932 16-17 25.689341024933082 24.648390305607936 24.43211301638773 25.230155653071257 18-19 24.979145738975145 24.049540129276835 25.038355196039845 25.93295893570817 20-21 24.967225521699202 24.697246062656774 24.79314629117248 25.542382124471548 22-23 25.08481222353418 24.768620505319305 25.176845112564056 24.969722158582456 24-25 25.018798207120994 24.161619543869335 25.739910160235134 25.079672088774537 26-27 24.851939642089967 24.229591706857626 26.064546385555143 24.85392226549726 28-29 25.017598842343748 24.401394591990787 25.829642227039262 24.751364338626203 30-31 24.892718044516503 24.304368429194398 25.884519284806544 24.918394241482552 32-33 24.843886764299857 24.32103715191497 25.98612261522224 24.848953468562936 34-35 25.001958146575102 24.375106474220022 25.791703137146627 24.831232242058245 36-37 25.09438266492 24.259331057967014 25.618676410404024 25.02760986670896 38-39 25.064569883314046 24.34421671199776 25.63216314494005 24.959050259748146 40-41 25.3690127220783 24.57407864308275 25.03416965773556 25.022738977103394 42-43 25.445551776332664 24.432308831045237 25.00839555344076 25.11374383918134 44-45 25.33161212249382 24.551413096475923 25.04148823056001 25.07548655047025 46-47 25.269245154076764 24.84234472387196 24.745073792753683 25.143336329297593 48-49 25.42797741082111 24.721380219194927 24.56191365748492 25.288728712499047 50-51 25.2803086822261 24.808738033276743 24.728160301711224 25.182792982785934 52-53 25.376967692539658 24.987198616765262 24.49345195785285 25.142381732842228 54-55 25.48921844495748 24.775963554975945 24.480209991638713 25.254608008427866 56-57 25.320646501673238 24.832260269010177 24.63664142615731 25.210451803159273 58-59 25.363211712849555 24.915726266773973 24.5285272583794 25.192534761997077 60-61 25.501848490366896 24.79706258432269 24.413902253239264 25.28718667207115 62-63 25.317611374481825 24.849565389367655 24.532859657676816 25.299963578473704 64-65 25.359882863671878 25.031183484208526 24.465793137479512 25.143140514640084 66-67 25.487627450865208 24.793023907011534 24.441365258955095 25.277983383168163 68-69 25.413438172500964 24.925394615488546 24.37207134702861 25.289095864981874 70-71 25.487015530060486 25.04601644451494 24.25211039247132 25.214857632953258 72-73 25.50786979108534 24.938098091394533 24.17415168195 25.379880435570122 74-75 25.366907714510063 24.941524847900965 24.46846111218809 25.223106325400877 76-77 25.47866893028411 25.091102769406703 24.211478851037917 25.21874944927128 78-79 25.571485077944022 24.871814829827272 24.23864813476748 25.318051957461225 80-81 25.346518513296797 24.933178248124584 24.39872661728221 25.32157662129641 82-83 25.451817845372997 25.167372578506992 24.22707059314218 25.15373898297783 84-85 25.48657494708109 25.04246730384756 24.195250711296744 25.275707037774602 86-87 25.295875947498175 25.119838570396347 24.271324705739524 25.312960776365955 88-89 25.44626160446614 25.240582783583683 24.073429517493103 25.239726094457076 90-91 25.45453477374595 25.099326985022135 24.06677181913775 25.379366422094158 92-93 25.274042613185767 25.197454605267023 24.18269409638389 25.34580868516332 94-95 25.48691762273173 25.343728154427268 24.021685494245986 25.14766872859501 96-97 25.388202558514315 25.283588577739398 23.992019573633165 25.33618929011312 98-99 25.34233297499251 25.303170043490436 24.07098183427422 25.28351514724283 100-101 25.357679948774887 25.392094374832336 23.864935860908933 25.385289815483848 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 306.0 1 236.5 2 146.5 3 100.5 4 70.0 5 64.5 6 53.5 7 43.5 8 40.5 9 40.5 10 44.5 11 43.0 12 38.5 13 37.5 14 43.5 15 55.0 16 76.5 17 95.0 18 100.0 19 123.5 20 153.0 21 227.5 22 358.5 23 566.5 24 854.5 25 1261.0 26 1824.0 27 2618.0 28 3573.0 29 4768.5 30 6320.0 31 8210.0 32 10366.0 33 13047.5 34 16037.0 35 18938.5 36 22807.5 37 26971.0 38 30651.0 39 34683.0 40 38785.0 41 42390.0 42 46752.5 43 52474.0 44 58768.5 45 64922.5 46 70828.5 47 77185.0 48 83185.0 49 86941.0 50 89024.5 51 91102.5 52 93842.0 53 96556.5 54 96435.0 55 94317.5 56 89020.0 57 81361.0 58 78159.5 59 74925.0 60 64724.5 61 54026.0 62 45174.0 63 35201.5 64 27257.5 65 21808.0 66 16328.5 67 11367.0 68 8597.5 69 7070.5 70 6300.0 71 6210.0 72 6045.5 73 4991.0 74 3837.0 75 3410.0 76 2805.5 77 1926.5 78 1279.0 79 727.0 80 286.5 81 248.0 82 211.0 83 81.5 84 15.5 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05140134759647299 2 2.447683218879666E-4 3 0.013560165032593349 4 0.003622571163941906 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2042748.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.58397771243865 #Duplication Level Percentage of deduplicated Percentage of total 1 89.03700889127259 63.73623260055059 2 5.791068752794989 8.29095473062553 3 1.8472982345205329 3.9671086694443534 4 1.0008100066365042 2.865678448378124 5 0.6132633104678024 2.1949913574191755 6 0.40291138663029974 1.7305199832378677 7 0.27781119615132055 1.392078133149344 8 0.1864695655420716 1.0678586579049418 9 0.14003403815336593 0.9021774119537997 >10 0.603123470803837 7.506178183146418 >50 0.0768797152764435 3.8735644362778108 >100 0.023045752282521145 2.3012655754237925 >500 2.0675960077291738E-4 0.12042815609780988 >1k 6.891986692430576E-5 0.05096365639042915 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 4.895366437759332E-5 6 0.0 0.0 0.0 0.0 9.790732875518664E-5 7 0.0 0.0 0.0 0.0 9.790732875518664E-5 8 4.895366437759332E-5 0.0 0.0 0.0 9.790732875518664E-5 9 2.9372198626555993E-4 0.0 0.0 4.895366437759332E-5 9.790732875518664E-5 10-11 5.629671403423232E-4 0.0 0.0 4.895366437759332E-5 9.790732875518664E-5 12-13 0.0010280269519294598 0.0 0.0 4.895366437759332E-5 2.6924515407676327E-4 14-15 0.0013462257703838164 0.0 0.0 4.895366437759332E-5 2.9372198626555993E-4 16-17 0.0023987295545020728 0.0 0.0 4.895366437759332E-5 2.9372198626555993E-4 18-19 0.005189088424024892 0.0 0.0 4.895366437759332E-5 3.4267565064315325E-4 20-21 0.008615844930456425 0.0 0.0 4.895366437759332E-5 4.405829793983399E-4 22-23 0.015077728628298741 0.0 0.0 4.895366437759332E-5 4.6505981158713656E-4 24-25 0.021955718473350604 0.0 0.0 9.790732875518664E-5 4.895366437759332E-4 26-27 0.02543142864415973 0.0 0.0 1.7133782532157663E-4 5.384903081535265E-4 28-29 0.02800149602398338 0.0 0.0 1.958146575103733E-4 5.384903081535265E-4 30-31 0.03027784141754147 0.0 0.0 1.958146575103733E-4 5.384903081535265E-4 32-33 0.03238284898577798 0.0 0.0 1.958146575103733E-4 5.384903081535265E-4 34-35 0.03483053220465765 0.0 0.0 2.447683218879666E-4 6.119208047199165E-4 36-37 0.03874682535486511 0.0 0.0 2.447683218879666E-4 6.363976369087132E-4 38-39 0.04393591377889 0.0 0.0 2.9372198626555993E-4 6.608744690975098E-4 40-41 0.05047122797329871 0.0 0.0 3.181988184543566E-4 6.853513012863065E-4 42-43 0.058597536259979204 0.0 0.0 3.4267565064315325E-4 7.098281334751032E-4 44-45 0.06679727504322608 0.0 0.0 3.916293150207466E-4 7.587817978526965E-4 46-47 0.07891330697668043 0.0 0.0 3.916293150207466E-4 7.832586300414932E-4 48-49 0.0953862150397406 0.0 0.0 3.916293150207466E-4 7.832586300414932E-4 50-51 0.11594675407832977 0.0 0.0 3.916293150207466E-4 7.832586300414932E-4 52-53 0.1407907387499584 0.0 0.0 3.916293150207466E-4 7.832586300414932E-4 54-55 0.16881671160613057 0.0 0.0 3.916293150207466E-4 8.322122944190865E-4 56-57 0.2072453381425413 0.0 0.0 4.6505981158713656E-4 8.811659587966798E-4 58-59 0.24878252236692927 0.0 0.0 4.895366437759332E-4 8.811659587966798E-4 60-61 0.30104055909001015 0.0 0.0 4.895366437759332E-4 8.811659587966798E-4 62-63 0.3608374601272404 0.0 0.0 4.895366437759332E-4 8.811659587966798E-4 64-65 0.4330685919163793 0.0 0.0 4.895366437759332E-4 9.056427909854765E-4 66-67 0.5127162038587236 0.0 0.0 4.895366437759332E-4 9.301196231742731E-4 68-69 0.6074904980937443 0.0 0.0 4.895366437759332E-4 9.545964553630698E-4 70-71 0.7087511528587961 0.0 0.0 4.895366437759332E-4 9.790732875518664E-4 72-73 0.8238167409783292 0.0 0.0 4.895366437759332E-4 9.790732875518664E-4 74-75 0.9541558723836714 0.0 0.0 5.384903081535265E-4 0.001003550119740663 76-77 1.1032687340778207 0.0 0.0 5.384903081535265E-4 0.0010280269519294598 78-79 1.2707392199135674 0.0 0.0 5.384903081535265E-4 0.0010280269519294598 80-81 1.4536790636926336 0.0 0.0 5.384903081535265E-4 0.0010525037841182564 82-83 1.6585501491128616 0.0 0.0 5.629671403423232E-4 0.001076980616307053 84-85 1.8842020650613782 0.0 0.0 5.874439725311199E-4 0.001076980616307053 86-87 2.1250785706313264 0.0 0.0 5.874439725311199E-4 0.001076980616307053 88-89 2.394005525889635 0.0 0.0 5.874439725311199E-4 0.0011014574484958497 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 1355 0.0 89.80014 1 TCGACGG 1470 0.0 83.37672 2 CGACGGC 1985 0.0 62.70226 3 ACGGCGC 2090 0.0 62.499374 5 GACGGCG 2175 0.0 59.841408 4 CGGCGCG 2515 0.0 52.12671 6 CGCGCCG 4480 0.0 37.321053 9 ATCTCGA 1105 0.0 33.98139 1 GGCGCGC 5105 0.0 32.751873 7 GCGCGCC 5220 0.0 32.03033 8 TCTCGAG 1440 0.0 26.72175 2 CTCGAGG 1785 0.0 22.621588 3 TCCATAT 3010 0.0 22.013899 18-19 ATCCATA 2995 0.0 21.965555 16-17 CCATATT 3075 0.0 21.6258 18-19 GGGCGCG 1950 0.0 21.192093 8 GCCGGAT 4280 0.0 20.753296 12-13 GTTGACG 350 3.6379788E-12 20.370401 1 CGGATCC 4385 0.0 20.039705 14-15 CGCCGGA 4255 0.0 19.982172 10-11 >>END_MODULE