FastQCFastQC Report
Tue 31 May 2016
SRR2048263_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048263_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1923274
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT87560.4552653444075051No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG51300.092.6854861
TCGACGG53900.088.388532
TATCTCG25350.085.81662
CGACGGC57900.082.11393
ACGGCGC58400.081.4921955
GACGGCG58650.081.063854
ATCTCGA27500.078.9304663
ATATCTC27900.077.804921
CGGCGCG66950.074.7031566
TCTCGAG27750.073.255794
CTCGAGG28750.070.542565
TCGAGGG31200.065.155396
AGGGCGC31750.063.8771179
CGAGGGC32050.063.1310127
GAGGGCG34150.059.5270358
CGCGCCG96100.052.7848179
GGCGCGC99500.051.1243257
GCGCGCC103450.049.2640848
ATCCATA61550.037.70306816-17
TCCATAT63000.037.47624218-19
Produced by FastQC (version 0.11.5)