##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048263_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1923274 Sequences flagged as poor quality 0 Sequence length 101 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.81062864677628 31.0 30.0 33.0 28.0 34.0 2 30.256257298752026 31.0 30.0 34.0 27.0 34.0 3 31.063955005890996 31.0 30.0 34.0 28.0 34.0 4 34.06690362371664 35.0 35.0 37.0 30.0 37.0 5 34.148548256774646 35.0 35.0 37.0 30.0 37.0 6 33.87595267237013 35.0 35.0 37.0 29.0 37.0 7 33.976973119794685 35.0 35.0 37.0 29.0 37.0 8 33.777804930550715 35.0 33.0 37.0 28.0 37.0 9 35.96270266223117 38.0 35.0 39.0 31.0 39.0 10-11 35.13852654379979 37.5 34.5 39.0 27.5 39.0 12-13 34.86752979554655 37.0 34.0 39.0 27.0 39.0 14-15 35.80011376434143 38.0 34.0 40.0 27.0 41.0 16-17 35.727073729484204 38.0 34.0 40.0 27.0 41.0 18-19 35.76606453370658 38.5 34.0 40.0 27.0 41.0 20-21 36.11751809674544 39.0 34.5 40.0 28.5 41.0 22-23 35.75892748511133 38.5 34.0 40.0 27.0 41.0 24-25 35.421247050602254 38.0 34.0 40.0 26.0 41.0 26-27 34.98582625252564 38.0 33.0 40.0 25.0 41.0 28-29 34.77966322011321 38.0 33.0 40.0 25.0 41.0 30-31 34.6027776073508 38.0 32.5 40.0 24.5 41.0 32-33 34.58736456687919 38.0 33.0 40.0 25.0 41.0 34-35 34.34083625110099 37.0 32.0 40.0 24.0 41.0 36-37 34.16033414895641 37.0 32.0 40.0 23.5 41.0 38-39 34.40399287880978 37.5 32.5 40.0 24.0 41.0 40-41 34.214069862120525 37.5 32.0 40.0 23.0 41.0 42-43 33.93479998169788 37.0 31.0 40.0 22.5 41.0 44-45 33.647960197039005 37.0 31.0 40.0 22.0 41.0 46-47 33.52681001250992 37.0 31.0 40.0 22.0 41.0 48-49 33.44312458859216 36.5 30.0 40.0 22.0 41.0 50-51 33.3224179186117 36.0 30.0 39.5 22.0 41.0 52-53 33.10534068468664 36.0 30.0 39.0 21.5 41.0 54-55 32.889338180623255 35.0 30.0 39.0 21.0 40.0 56-57 33.30056091851707 36.0 31.0 39.0 22.0 41.0 58-59 33.25971780411943 35.0 31.0 39.0 22.0 41.0 60-61 33.15690042084487 35.0 31.0 39.0 22.5 41.0 62-63 32.9720676825039 35.0 31.0 39.0 22.5 41.0 64-65 32.70096148546697 35.0 31.0 39.0 22.5 40.0 66-67 32.38505459960463 35.0 30.5 38.0 22.0 40.0 68-69 32.135511112821156 35.0 30.0 37.0 22.0 40.0 70-71 31.865262307918684 35.0 30.0 37.0 22.0 39.0 72-73 31.60608732817061 34.0 30.0 36.0 22.0 39.0 74-75 31.31606260990374 34.0 30.0 36.0 20.5 38.5 76-77 31.10779743291907 34.0 30.0 35.0 20.0 37.0 78-79 30.986761896640832 34.0 30.0 35.0 21.0 37.0 80-81 30.82343805406822 34.0 30.0 35.0 20.5 36.5 82-83 30.746037226105067 34.0 30.0 35.0 22.0 36.0 84-85 30.70218154043574 34.0 30.0 35.0 23.0 36.0 86-87 30.58894780462898 34.0 30.0 35.0 23.0 35.0 88-89 30.512021688017413 34.0 30.0 35.0 23.0 35.0 90-91 30.50711443091312 34.0 30.0 35.0 23.0 35.0 92-93 30.494814311429366 34.0 30.0 35.0 23.0 35.0 94-95 30.409152570044622 34.0 30.0 35.0 23.0 35.0 96-97 30.314120088973283 34.0 30.0 35.0 23.0 35.0 98-99 30.177329127311033 34.0 30.0 35.0 20.0 35.0 100-101 29.252734919725427 33.5 29.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.18549700760991783 1101 2 0.2336643021263214 1101 3 0.2940073943223318 1101 4 -0.10108172089315559 1101 5 -0.15550733863800303 1101 6 0.09188226711754766 1101 7 -0.10480914528957896 1101 8 0.03967693316859311 1101 9 0.8440231176971196 1101 10-11 0.21657615424785348 1101 12-13 0.22344418029000224 1101 14-15 0.01364962674391279 1101 16-17 -0.2780095498857236 1101 18-19 -0.005079012848632658 1101 20-21 0.9876036151151197 1101 22-23 0.33084288467301803 1101 24-25 0.18686824761395826 1101 26-27 0.8671474803155874 1101 28-29 0.8568743387475308 1101 30-31 0.7047080808277926 1101 32-33 0.8921273483352437 1101 34-35 0.6629860607424263 1101 36-37 0.7667108011451234 1101 38-39 0.7485883491778296 1101 40-41 0.5684146342451797 1101 42-43 0.7352682173156211 1101 44-45 0.6696336024287959 1101 46-47 0.6224812891788254 1101 48-49 0.675755181668201 1101 50-51 0.3158529358523694 1101 52-53 0.3716453989960442 1101 54-55 0.4958875161352161 1101 56-57 0.5471484186300444 1101 58-59 0.6294830194134242 1101 60-61 0.7896884963595383 1101 62-63 0.6750340561138408 1101 64-65 0.8081805396434341 1101 66-67 1.5561630986728616 1101 68-69 1.488425136385409 1101 70-71 2.1136502117654636 1101 72-73 1.4330117904176447 1101 74-75 1.0111617689220047 1101 76-77 1.3229191172384738 1101 78-79 1.710309544551901 1101 80-81 2.244376845542263 1101 82-83 2.081193928923671 1101 84-85 1.9834543725678948 1101 86-87 1.891450293981876 1101 88-89 1.9799295061259272 1101 90-91 1.785246184589493 1101 92-93 1.6609209644646619 1101 94-95 1.678351413767544 1101 96-97 1.7391496257058776 1101 98-99 1.7201253415280995 1101 100-101 1.8705627158552218 1102 1 1.1767113650654224 1102 2 2.1581641949573367 1102 3 1.1948325364614725 1102 4 2.2657032449810544 1102 5 2.0559823870152982 1102 6 2.625831216891015 1102 7 2.32294818279572 1102 8 2.699962321518882 1102 9 2.2085348020688116 1102 10-11 2.963199814561733 1102 12-13 3.0450540975838862 1102 14-15 3.4506913907631187 1102 16-17 3.3241512041824137 1102 18-19 3.3178056396670073 1102 20-21 3.3571881862059527 1102 22-23 3.5021266746406994 1102 24-25 3.51931500116131 1102 26-27 4.265929695223939 1102 28-29 4.330827477854058 1102 30-31 4.315711309477983 1102 32-33 4.303415268589703 1102 34-35 4.1678141268529885 1102 36-37 4.525600881784612 1102 38-39 4.651884651641183 1102 40-41 4.708547455883519 1102 42-43 4.624990150703155 1102 44-45 4.720469343373914 1102 46-47 4.795476609171242 1102 48-49 4.5968768896170715 1102 50-51 4.521694516473858 1102 52-53 4.561947476775341 1102 54-55 4.585796759061619 1102 56-57 4.496532846474615 1102 58-59 4.557021285826661 1102 60-61 4.478058063438176 1102 62-63 4.437117432993805 1102 64-65 4.375688290942897 1102 66-67 4.304695429754759 1102 68-69 4.091177386491246 1102 70-71 3.9717499137589307 1102 72-73 3.866139007974226 1102 74-75 3.8224538667002683 1102 76-77 3.602477090935718 1102 78-79 3.3902160093511746 1102 80-81 3.242446770500358 1102 82-83 3.124895699189981 1102 84-85 2.9753334434393643 1102 86-87 2.7939708022285785 1102 88-89 2.780847764237258 1102 90-91 2.6234134886606704 1102 92-93 2.477688644542507 1102 94-95 2.4127973707806767 1102 96-97 2.423804296531763 1102 98-99 2.403798441876649 1102 100-101 2.348735227807662 1103 1 1.154848979377661 1103 2 2.0658469582887697 1103 3 1.1692923577765022 1103 4 2.2569433942364086 1103 5 1.9993185785330283 1103 6 2.3676390669671363 1103 7 2.2370427862771365 1103 8 2.730688106580992 1103 9 2.091195129863806 1103 10-11 2.907228146920133 1103 12-13 3.1367718421857305 1103 14-15 3.3199262753881413 1103 16-17 3.217958905293841 1103 18-19 3.2053418362713373 1103 20-21 3.391307459694225 1103 22-23 3.4707950392626756 1103 24-25 3.583415690395519 1103 26-27 4.3565288421285615 1103 28-29 4.36647681859445 1103 30-31 4.3060653912923 1103 32-33 4.261661554463856 1103 34-35 4.236110806393597 1103 36-37 4.429273406518206 1103 38-39 4.438194341976278 1103 40-41 4.409592116225845 1103 42-43 4.454724866380417 1103 44-45 4.474929655182443 1103 46-47 4.441936608517249 1103 48-49 4.237047218029723 1103 50-51 4.152376066288014 1103 52-53 4.28833293927438 1103 54-55 4.267457842579947 1103 56-57 4.315593424239637 1103 58-59 4.3912817124470145 1103 60-61 4.193223032755078 1103 62-63 4.1613179285739115 1103 64-65 4.142083490646414 1103 66-67 4.109793746453704 1103 68-69 3.894131610730348 1103 70-71 3.8956607456722487 1103 72-73 3.6640733944758423 1103 74-75 3.735430326645119 1103 76-77 3.5497172474576466 1103 78-79 3.345568383521421 1103 80-81 3.2230200484139786 1103 82-83 3.0245816016798663 1103 84-85 2.9008774370738486 1103 86-87 2.876534938796503 1103 88-89 2.881094191809538 1103 90-91 2.7169982877218324 1103 92-93 2.695918744130857 1103 94-95 2.541007378301579 1103 96-97 2.6053109654268454 1103 98-99 2.6508877262284543 1103 100-101 2.717861113875106 1104 1 -0.17994660510330007 1104 2 -0.3525858233770869 1104 3 -0.209024675052234 1104 4 -0.6884195426002222 1104 5 -0.50248335726085 1104 6 -1.0805640639065217 1104 7 -0.8560269931757318 1104 8 -0.43212802895727265 1104 9 0.29497731546074135 1104 10-11 -0.26436987184941785 1104 12-13 -1.0200366148995954 1104 14-15 -1.2159449135789373 1104 16-17 -1.1500932225430844 1104 18-19 -1.0852282782353413 1104 20-21 -0.3683549613229573 1104 22-23 -1.0538708467013507 1104 24-25 -1.316224249327476 1104 26-27 -1.203593910150623 1104 28-29 -0.8458713039600667 1104 30-31 -1.1307399849322763 1104 32-33 -1.1585455823459725 1104 34-35 -1.070640160359254 1104 36-37 -1.1212218602336712 1104 38-39 -1.3298570912368675 1104 40-41 -1.1615373583770747 1104 42-43 -1.1011947991541469 1104 44-45 -1.1832715225725963 1104 46-47 -1.2988085511808443 1104 48-49 -1.5212905145875197 1104 50-51 -1.531731309472395 1104 52-53 -1.644998909470882 1104 54-55 -1.6263339778573531 1104 56-57 -1.2906639918906713 1104 58-59 -1.1393536476622934 1104 60-61 -0.9408085064331431 1104 62-63 -1.003701390139092 1104 64-65 -0.9768177126054027 1104 66-67 -0.5898010835841205 1104 68-69 -0.6207656176390728 1104 70-71 -0.8536858993766323 1104 72-73 -0.6837837728974279 1104 74-75 -0.7082277174250855 1104 76-77 -0.663371262321018 1104 78-79 -0.9361673423685986 1104 80-81 -0.712455327581786 1104 82-83 -0.7618338454453699 1104 84-85 -0.7466428786285668 1104 86-87 -0.7578185789417375 1104 88-89 -0.7204926966355174 1104 90-91 -0.5422005740758244 1104 92-93 -0.20746337903165823 1104 94-95 -0.27744187003377974 1104 96-97 -0.1227148157388811 1104 98-99 -0.003924504706986909 1104 100-101 0.2567506474475003 1105 1 -0.9415049022010855 1105 2 -1.637344140699362 1105 3 -0.8572171110922788 1105 4 -1.9355350244487397 1105 5 -1.6037777247398672 1105 6 -2.2386437484044492 1105 7 -1.5317070417145082 1105 8 -1.7388804811769099 1105 9 -1.5380088307623296 1105 10-11 -2.4006235375385643 1105 12-13 -2.4649855480576477 1105 14-15 -2.6205485135384876 1105 16-17 -2.532184435001369 1105 18-19 -2.3234272872352406 1105 20-21 -2.280880241937787 1105 22-23 -2.956508878858287 1105 24-25 -2.8498702195974737 1105 26-27 -3.750485658557942 1105 28-29 -3.2973805566650967 1105 30-31 -3.190461645363058 1105 32-33 -3.351023335404925 1105 34-35 -3.5274848868230038 1105 36-37 -3.46015274303274 1105 38-39 -3.263729323009546 1105 40-41 -3.2683707085641487 1105 42-43 -3.4958530052417203 1105 44-45 -3.3192268770068125 1105 46-47 -3.645302129904678 1105 48-49 -3.741217959222972 1105 50-51 -3.4438318757581676 1105 52-53 -3.2516919522456824 1105 54-55 -2.8554104769978146 1105 56-57 -2.353832664152783 1105 58-59 -2.544126627741168 1105 60-61 -2.6306949964725845 1105 62-63 -2.8337195921817653 1105 64-65 -2.556909228431067 1105 66-67 -2.6469833062557733 1105 68-69 -2.673109130633307 1105 70-71 -2.390173410752908 1105 72-73 -2.3041055951241454 1105 74-75 -2.02054822505983 1105 76-77 -2.3678560433908977 1105 78-79 -2.3768413235598196 1105 80-81 -2.3294502208975914 1105 82-83 -2.4836914650226802 1105 84-85 -2.274086250051365 1105 86-87 -2.4606917208165484 1105 88-89 -2.3280730889906636 1105 90-91 -2.411514963801782 1105 92-93 -2.4442580498780515 1105 94-95 -2.315576413465873 1105 96-97 -2.2962662365094353 1105 98-99 -2.4898798591412046 1105 100-101 -2.543310026028081 1106 1 0.8478079213274157 1106 2 1.7522588429201313 1106 3 0.832416049176679 1106 4 1.776927931779639 1106 5 1.5598957793881425 1106 6 1.9631142348668504 1106 7 1.8722515156868909 1106 8 2.379073077733331 1106 9 1.9392689259519713 1106 10-11 2.4733939803933325 1106 12-13 2.4977188213664405 1106 14-15 2.431519747590116 1106 16-17 2.3199851887190235 1106 18-19 2.4576535010716896 1106 20-21 2.759680269981992 1106 22-23 2.7242295205412717 1106 24-25 2.6335542884844543 1106 26-27 3.4973799238127015 1106 28-29 3.5883779991887863 1106 30-31 3.5646509754402587 1106 32-33 3.4899948556983773 1106 34-35 3.6559767617505727 1106 36-37 3.83817477420277 1106 38-39 3.950161994626775 1106 40-41 3.679676320583397 1106 42-43 3.726598808839171 1106 44-45 3.8354234220515195 1106 46-47 3.889616896085929 1106 48-49 3.659485388837602 1106 50-51 3.758919615664695 1106 52-53 3.796873883144567 1106 54-55 3.7996638426734926 1106 56-57 3.7590744304548807 1106 58-59 3.8889558783135065 1106 60-61 3.7098508140025857 1106 62-63 3.755152961768662 1106 64-65 3.6839015949272067 1106 66-67 3.5573447216614937 1106 68-69 3.4010503723031107 1106 70-71 3.390978950376468 1106 72-73 3.1504858981557433 1106 74-75 3.1730504564527173 1106 76-77 3.1776747174475553 1106 78-79 3.0803546466968363 1106 80-81 2.8222783594844927 1106 82-83 2.700098644773899 1106 84-85 2.5534151233730675 1106 86-87 2.4468996409241015 1106 88-89 2.4562679380986836 1106 90-91 2.3357710721471214 1106 92-93 2.318238951801007 1106 94-95 2.2516409125734818 1106 96-97 2.2151525302714887 1106 98-99 2.2583060599698968 1106 100-101 2.248698191305696 1107 1 -0.6670791986546902 1107 2 -1.999205141972073 1107 3 -0.7555803986665985 1107 4 -1.8094187440270773 1107 5 -1.9436533726902354 1107 6 -1.8439342144587165 1107 7 -1.9182284372180973 1107 8 -1.7651282378555422 1107 9 -0.2118641029849755 1107 10-11 -1.5654822716807146 1107 12-13 -2.159567439955225 1107 14-15 -2.6665955060964137 1107 16-17 -2.9072622014139498 1107 18-19 -2.640451792776908 1107 20-21 -1.546392894208445 1107 22-23 -2.4548972743161173 1107 24-25 -2.6150646325368143 1107 26-27 -3.029402581147739 1107 28-29 -3.0342253243924375 1107 30-31 -3.2243825196616136 1107 32-33 -3.060685417924521 1107 34-35 -3.2334329815304343 1107 36-37 -3.0465393228071633 1107 38-39 -3.373570129133398 1107 40-41 -3.746065631700631 1107 42-43 -3.965751868708015 1107 44-45 -4.116441015095226 1107 46-47 -4.315163269703639 1107 48-49 -4.057787307073525 1107 50-51 -4.011721119070877 1107 52-53 -4.185698292295477 1107 54-55 -4.2679520503344754 1107 56-57 -3.988509001271261 1107 58-59 -3.8718832074290113 1107 60-61 -3.5777756175154636 1107 62-63 -3.589480243552 1107 64-65 -3.658044401607434 1107 66-67 -3.7639830749664043 1107 68-69 -3.674217959084622 1107 70-71 -3.7296804799207983 1107 72-73 -3.7152339533035637 1107 74-75 -3.688385273659044 1107 76-77 -3.525758533276626 1107 78-79 -3.4776119995308328 1107 80-81 -3.28438020948154 1107 82-83 -3.209473427172856 1107 84-85 -3.0231857675594718 1107 86-87 -2.8138364523044324 1107 88-89 -2.5063505644981063 1107 90-91 -2.335716860869354 1107 92-93 -2.0171118514960895 1107 94-95 -1.3907990140241857 1107 96-97 -0.7585988454658974 1107 98-99 -0.1752846340916996 1107 100-101 0.1759896495483808 1108 1 -0.1908422791684785 1108 2 -0.8818081398508291 1108 3 -0.3726936359502169 1108 4 -1.0571441760516294 1108 5 -0.16985166117441253 1108 6 -0.968485649907656 1108 7 -0.340923070376121 1108 8 -0.7838858139812146 1108 9 0.35327129761780895 1108 10-11 -0.3059388604629447 1108 12-13 0.3832823217472878 1108 14-15 -1.1593802660178056 1108 16-17 -0.0937881457689258 1108 18-19 -1.0812053247239746 1108 20-21 0.07139430222039067 1108 22-23 -0.8670046823439606 1108 24-25 -0.9618135406283628 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1.4860733642258879 2216 88-89 1.4477375874186187 2216 90-91 1.372800499030479 2216 92-93 1.3700355602918464 2216 94-95 1.2690668780613557 2216 96-97 1.2461290137341692 2216 98-99 1.3165408236013931 2216 100-101 1.3702720354089841 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 36.0 11 575.0 12 2708.0 13 5906.0 14 7738.0 15 8347.0 16 8484.0 17 8395.0 18 9430.0 19 13108.0 20 15188.0 21 18457.0 22 25518.0 23 33932.0 24 42968.0 25 50906.0 26 56942.0 27 58944.0 28 60962.0 29 62865.0 30 65003.0 31 69942.0 32 81095.0 33 97993.0 34 128968.0 35 185641.0 36 282784.0 37 333583.0 38 173024.0 39 13827.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.571547421397643 34.839457090370026 12.64324485374063 23.945750634491702 2 26.23391439452398 24.22829652216054 28.200221719805235 21.337567363510246 3 23.808516103269735 26.174273660435276 21.979707519573395 28.03750271672159 4 26.330725627237722 22.17011200692153 23.79380161121088 27.705360754629865 5 24.533425814522527 25.108642866279062 23.482613501768338 26.875317817430073 6 23.969231633142236 20.69309937117644 25.09684007582903 30.24082891985229 7 27.222070282237475 22.30680599852127 23.10705598890226 27.36406773033899 8 27.05490741308831 20.714833143899412 24.778632685722368 27.451626757289915 9 23.14314029098298 23.73478765896071 25.266030737170052 27.856041312886255 10-11 26.540680111102215 20.89268091805952 23.879592819327875 28.687046151510394 12-13 26.075769755115495 20.960611956486698 23.82299661930645 29.14062166909135 14-15 25.854246456823105 21.00361154988837 23.929533701386283 29.21260829190225 16-17 26.001859329455918 22.256241180403833 23.93010564277373 27.811793847366523 18-19 24.452704086885177 20.704304222903236 25.38463058305785 29.458361107153742 20-21 24.570012944393184 24.013656217819378 24.042565969218916 27.37376486856852 22-23 24.096212012098515 22.355425379742524 25.934569841790626 27.613792766368327 24-25 24.042472378473473 20.946604838053453 27.290320383450407 27.72060240002267 26-27 23.966657896898727 21.15361617741414 28.161588000461713 26.71813792522542 28-29 24.10940926773824 21.23358398231349 27.995231048722125 26.661775701226137 30-31 24.066513663679746 21.14597296069099 27.93559316041292 26.851920215216346 32-33 23.964526115363697 21.323508766821575 28.06196620970283 26.6499989081119 34-35 24.196318361294335 21.364090607994495 27.86459443636216 26.57499659434901 36-37 24.2176613604316 21.331226160418286 27.65602057327305 26.795091905877065 38-39 24.37307424735113 21.33226986898383 27.51651610742931 26.778139776235733 40-41 25.06140570714313 21.663761897680725 25.93200448818005 27.342827906996092 42-43 25.160975503230432 21.57908857500283 25.846655234771543 27.413280686995193 44-45 25.075964647676653 21.829381074344344 25.796208566539885 27.29844571143912 46-47 24.893656847375933 22.18475969125259 25.585608772627655 27.335974688743825 48-49 24.99951254779956 22.241910958211438 25.257355263742838 27.501221230246166 50-51 24.842664123780594 22.49791241393582 25.327670420335323 27.33175304194826 52-53 25.001247872118064 22.70524116688522 25.13305436458872 27.160456596408 54-55 25.155373158152795 22.49737816732206 25.083620474056467 27.26362820046868 56-57 24.957006894755217 22.805102862385407 25.30154967559216 26.936340567267216 58-59 24.970213327946645 22.969859241563604 25.17533385380392 26.884593576685827 60-61 25.108954834308577 23.031819699117236 25.01214075581534 26.84708471075884 62-63 25.05118886063839 23.10562219553157 25.02940304793399 26.813785895896054 64-65 25.093622929864107 23.378864212500197 24.92414625376994 26.60336660386575 66-67 25.246046306523308 23.154041680447133 24.82283001874143 26.777081994288128 68-69 25.200262892167412 23.29658250581576 24.753122624429214 26.750031977587614 70-71 25.183691391702606 23.405596676279654 24.61458149455627 26.796130437461475 72-73 25.32455209003851 23.214729527792827 24.54665657272577 26.914061809442895 74-75 25.28587327803352 23.2200464468522 24.73327376475577 26.76080651035851 76-77 25.31462633889564 23.37868223110234 24.554282061287694 26.752409368714332 78-79 25.390954536627262 23.308775376980964 24.58885852688138 26.71141155951039 80-81 25.32622115367367 23.30030024330913 24.76543247749215 26.608046125525046 82-83 25.434396096031065 23.633872145589276 24.637317573397645 26.294414184982013 84-85 25.541810475262494 23.610520393870036 24.563712710721404 26.283956420146065 86-87 25.39876263785122 23.713074464032907 24.593138217174086 26.295024680941786 88-89 25.50269912199241 23.742186273214998 24.60547894206481 26.149635662727782 90-91 25.61012704036455 23.881201946499854 24.482866735265933 26.025804277869664 92-93 25.48473559285902 24.015463287690466 24.65146508671452 25.848336032735997 94-95 25.72779617235628 24.198137340948133 24.498562865782894 25.57550362091269 96-97 25.736733340421953 24.174680576653156 24.411776710556314 25.676809372368574 98-99 25.79188037776592 24.27957402413461 24.542227770687656 25.38631782741182 100-101 25.86673190266491 24.399337899679896 24.247019469669812 25.486910727985386 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 396.0 1 379.0 2 313.5 3 195.5 4 102.0 5 66.0 6 55.0 7 48.0 8 41.0 9 38.0 10 38.5 11 39.5 12 32.5 13 34.5 14 41.5 15 67.5 16 517.0 17 553.5 18 167.5 19 172.5 20 194.0 21 270.5 22 353.0 23 488.5 24 701.5 25 961.5 26 1351.0 27 1983.5 28 2745.5 29 3699.5 30 4892.5 31 6357.0 32 8267.5 33 10435.0 34 12931.0 35 15971.0 36 19328.5 37 22713.5 38 26380.0 39 30192.0 40 33669.5 41 37095.0 42 41268.5 43 46072.0 44 51397.0 45 56978.5 46 61903.0 47 66655.0 48 71677.5 49 75124.0 50 77091.0 51 79051.5 52 81251.5 53 83278.5 54 83719.5 55 82636.0 56 78932.5 57 73510.5 58 70502.5 59 67780.5 60 60472.5 61 52272.5 62 45589.0 63 38411.0 64 32571.5 65 28368.5 66 23996.0 67 19844.5 68 17143.0 69 15244.0 70 13721.0 71 12649.5 72 11666.0 73 10385.0 74 8995.5 75 7969.0 76 6947.0 77 5624.0 78 4422.0 79 3527.5 80 2795.5 81 2191.5 82 1668.0 83 1214.0 84 843.0 85 581.5 86 405.5 87 296.0 88 198.0 89 119.5 90 81.0 91 51.0 92 33.0 93 25.0 94 15.0 95 5.5 96 2.0 97 3.0 98 3.5 99 3.5 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0042115683984705245 2 0.006551328619843038 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0024697469003376535 22-23 0.003275664309921519 24-25 2.599733579302793E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 1.0398934317211172E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.009125064863352805 46-47 9.878987601350613E-4 48-49 4.419547084814748E-4 50-51 0.0 52-53 0.0 54-55 5.199467158605586E-5 56-57 2.599733579302793E-5 58-59 7.5392273799781E-4 60-61 0.0 62-63 2.0797868634422345E-4 64-65 2.599733579302793E-5 66-67 0.013622603955546635 68-69 0.0027037229224749046 70-71 3.8996003689541897E-4 72-73 4.159573726884469E-4 74-75 2.599733579302793E-5 76-77 2.599733579302793E-5 78-79 2.599733579302793E-5 80-81 2.599733579302793E-5 82-83 2.599733579302793E-5 84-85 0.0 86-87 0.01203676647217193 88-89 0.002911701608819128 90-91 0.0014038561328235083 92-93 4.6795204427450273E-4 94-95 1.0398934317211172E-4 96-97 1.819813505511955E-4 98-99 0.0010138960959280893 100-101 2.599733579302793E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1923274.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.03523041950385 #Duplication Level Percentage of deduplicated Percentage of total 1 90.89602826694063 70.02196481761507 2 4.994452368820728 7.69497578102683 3 1.6365807536939372 3.782231263828131 4 0.8011055548119098 2.468534040211197 5 0.4912773367582472 1.8922831418525883 6 0.2976175545056289 1.3756222132938218 7 0.19418090053052078 1.0471139290804798 8 0.13369237964072017 0.823921861676373 9 0.10190588744108837 0.7065309168115439 >10 0.38899327786043986 5.678469701676291 >50 0.05414615751282562 2.8168830178516724 >100 0.009745299400053526 1.1070203640600695 >500 2.0569656249234334E-4 0.12196047247139065 >1k 0.0 0.0 >5k 6.856552083078095E-5 0.46248847854460573 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 8756 0.4552653444075051 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 5.199467158605586E-5 0.0 4 5.199467158605586E-5 0.0 0.0 5.199467158605586E-5 0.0 5 5.199467158605586E-5 0.0 0.0 5.199467158605586E-5 5.199467158605586E-5 6 5.199467158605586E-5 0.0 0.0 5.199467158605586E-5 5.199467158605586E-5 7 1.0398934317211172E-4 0.0 0.0 5.199467158605586E-5 1.0398934317211172E-4 8 1.0398934317211172E-4 0.0 0.0 5.199467158605586E-5 1.0398934317211172E-4 9 2.0797868634422345E-4 0.0 0.0 5.199467158605586E-5 1.5598401475816758E-4 10-11 2.599733579302793E-4 0.0 0.0 5.199467158605586E-5 1.819813505511955E-4 12-13 3.63962701102391E-4 0.0 0.0 5.199467158605586E-5 2.0797868634422345E-4 14-15 0.0010398934317211173 0.0 0.0 5.199467158605586E-5 2.339760221372514E-4 16-17 0.0018718081770980111 0.0 0.0 1.0398934317211172E-4 2.599733579302793E-4 18-19 0.002911701608819128 5.199467158605586E-5 0.0 1.0398934317211172E-4 3.6396270110239105E-4 20-21 0.004627525771158972 5.199467158605586E-5 5.199467158605586E-5 1.0398934317211172E-4 3.8996003689541897E-4 22-23 0.006993283328324514 5.199467158605586E-5 5.199467158605586E-5 1.0398934317211172E-4 4.419547084814748E-4 24-25 0.009229054206524916 5.199467158605586E-5 5.199467158605586E-5 1.0398934317211172E-4 4.939493800675307E-4 26-27 0.010710902346727507 5.199467158605586E-5 5.199467158605586E-5 1.0398934317211172E-4 5.719413874466145E-4 28-29 0.011906779793206791 5.199467158605586E-5 5.199467158605586E-5 1.5598401475816758E-4 6.239360590326703E-4 30-31 0.013362630597616356 5.199467158605586E-5 5.199467158605586E-5 1.5598401475816758E-4 6.239360590326703E-4 32-33 0.014688494723060781 5.199467158605586E-5 5.199467158605586E-5 1.5598401475816758E-4 6.499333948256983E-4 34-35 0.015598401475816759 5.199467158605586E-5 5.199467158605586E-5 1.5598401475816758E-4 7.279254022047821E-4 36-37 0.017158241623398435 5.199467158605586E-5 5.199467158605586E-5 1.5598401475816758E-4 7.279254022047821E-4 38-39 0.01960199118794306 5.199467158605586E-5 5.199467158605586E-5 1.5598401475816758E-4 7.5392273799781E-4 40-41 0.022331711446210992 5.199467158605586E-5 5.199467158605586E-5 1.5598401475816758E-4 8.839094169629496E-4 42-43 0.02649128517309546 5.199467158605586E-5 5.199467158605586E-5 1.5598401475816758E-4 9.878987601350613E-4 44-45 0.03150877098114985 7.799200737908379E-5 5.199467158605586E-5 1.5598401475816758E-4 9.878987601350613E-4 46-47 0.038398064966302256 1.0398934317211172E-4 5.199467158605586E-5 1.5598401475816758E-4 0.0010398934317211173 48-49 0.047107172456966606 1.0398934317211172E-4 5.199467158605586E-5 1.5598401475816758E-4 0.001169880110686257 50-51 0.05675218403617997 1.0398934317211172E-4 5.199467158605586E-5 1.5598401475816758E-4 0.0012218747822723129 52-53 0.06962086525372879 1.0398934317211172E-4 5.199467158605586E-5 1.5598401475816758E-4 0.0012478721180653406 54-55 0.08636314950443878 1.5598401475816758E-4 5.199467158605586E-5 1.5598401475816758E-4 0.0012478721180653406 56-57 0.10645909007244937 1.5598401475816758E-4 5.199467158605586E-5 1.5598401475816758E-4 0.0012478721180653406 58-59 0.13040263633782812 1.5598401475816758E-4 5.199467158605586E-5 1.819813505511955E-4 0.0012998667896513966 60-61 0.15840176698691918 1.5598401475816758E-4 5.199467158605586E-5 2.0797868634422345E-4 0.0013778587970304803 62-63 0.19206831683889036 1.5598401475816758E-4 5.199467158605586E-5 2.0797868634422345E-4 0.0014038561328235083 64-65 0.23096033118526013 1.5598401475816758E-4 5.199467158605586E-5 2.0797868634422345E-4 0.0014818481402025922 66-67 0.27406391393010043 1.5598401475816758E-4 5.199467158605586E-5 2.0797868634422345E-4 0.001533842811788648 68-69 0.32525266810657244 1.5598401475816758E-4 5.199467158605586E-5 2.0797868634422345E-4 0.0016118348191677316 70-71 0.38780225802459767 1.5598401475816758E-4 5.199467158605586E-5 2.0797868634422345E-4 0.0016378321549607596 72-73 0.4559412751381238 1.5598401475816758E-4 5.199467158605586E-5 2.0797868634422345E-4 0.0016638294907537876 74-75 0.531515530288456 1.5598401475816758E-4 5.199467158605586E-5 2.0797868634422345E-4 0.0016638294907537876 76-77 0.6214923094681257 1.5598401475816758E-4 5.199467158605586E-5 2.0797868634422345E-4 0.0016638294907537876 78-79 0.7229079163967277 1.5598401475816758E-4 5.199467158605586E-5 2.0797868634422345E-4 0.0016638294907537876 80-81 0.8369062338491551 1.5598401475816758E-4 5.199467158605586E-5 2.0797868634422345E-4 0.0016898268265468155 82-83 0.9658530193825736 1.5598401475816758E-4 5.199467158605586E-5 2.0797868634422345E-4 0.0017158241623398435 84-85 1.1080064514988504 1.5598401475816758E-4 5.199467158605586E-5 2.0797868634422345E-4 0.0017158241623398435 86-87 1.262144655415713 1.5598401475816758E-4 5.199467158605586E-5 2.0797868634422345E-4 0.0017158241623398435 88-89 1.4396804615463008 1.5598401475816758E-4 5.199467158605586E-5 2.0797868634422345E-4 0.0017418214981328715 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 5130 0.0 92.685486 1 TCGACGG 5390 0.0 88.38853 2 TATCTCG 2535 0.0 85.8166 2 CGACGGC 5790 0.0 82.1139 3 ACGGCGC 5840 0.0 81.492195 5 GACGGCG 5865 0.0 81.06385 4 ATCTCGA 2750 0.0 78.930466 3 ATATCTC 2790 0.0 77.80492 1 CGGCGCG 6695 0.0 74.703156 6 TCTCGAG 2775 0.0 73.25579 4 CTCGAGG 2875 0.0 70.54256 5 TCGAGGG 3120 0.0 65.15539 6 AGGGCGC 3175 0.0 63.877117 9 CGAGGGC 3205 0.0 63.131012 7 GAGGGCG 3415 0.0 59.527035 8 CGCGCCG 9610 0.0 52.784817 9 GGCGCGC 9950 0.0 51.124325 7 GCGCGCC 10345 0.0 49.264084 8 ATCCATA 6155 0.0 37.703068 16-17 TCCATAT 6300 0.0 37.476242 18-19 >>END_MODULE