FastQCFastQC Report
Tue 31 May 2016
SRR2048263_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048263_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1923274
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG15600.090.8205951
TCGACGG16600.085.5846562
TATCTCG6650.067.878892
ACGGCGC23200.067.563475
CGACGGC21650.066.938313
GACGGCG24000.064.7209854
CGGCGCG27050.057.771636
ATATCTC9750.044.374091
CGCGCCG48450.039.2159
ATCTCGA11650.038.7463233
GGCGCGC53200.035.6243747
GCGCGCC54600.034.9719128
TCTCGAG15450.031.6677594
GTTGACG3500.031.242981
CCATATT31500.025.70986618-19
ATCCATA31550.025.36801716-17
TCCATAT31350.025.2268318-19
TTGACGG4650.024.5239892
CTCGAGG20350.024.274765
TGACGGC4900.023.2727643
Produced by FastQC (version 0.11.5)