##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048263_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1923274 Sequences flagged as poor quality 0 Sequence length 101 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.83138907924716 31.0 28.0 33.0 16.0 34.0 2 30.66644170305427 31.0 30.0 33.0 27.0 34.0 3 28.531440657961372 31.0 28.0 33.0 16.0 34.0 4 33.00427448195109 35.0 33.0 37.0 25.0 37.0 5 34.25908476899287 35.0 35.0 37.0 32.0 37.0 6 34.900366250466654 35.0 35.0 37.0 32.0 37.0 7 35.11678523184944 35.0 35.0 37.0 33.0 37.0 8 35.270383211128525 35.0 35.0 37.0 33.0 37.0 9 37.04702606076929 39.0 37.0 39.0 34.0 39.0 10-11 37.04615177036657 39.0 37.0 39.0 34.0 39.0 12-13 36.99697417008705 39.0 37.0 39.0 34.0 39.0 14-15 38.005590987035646 39.0 37.0 40.0 34.0 41.0 16-17 37.93574888445432 39.0 37.0 40.0 34.0 41.0 18-19 38.106112285612966 39.0 37.0 40.0 34.0 41.0 20-21 38.14631820531032 39.0 37.0 40.0 34.0 41.0 22-23 38.07706260262448 39.0 37.0 40.0 34.0 41.0 24-25 38.01320066719562 39.0 37.0 40.0 34.0 41.0 26-27 38.273859834844124 39.0 37.5 40.0 34.5 41.0 28-29 38.465113915125976 39.0 38.0 40.0 35.0 41.0 30-31 38.41296065979158 39.0 38.0 40.0 35.0 41.0 32-33 38.383101419766504 40.0 38.0 40.0 35.0 41.0 34-35 38.35242482350409 40.0 38.0 40.0 35.0 41.0 36-37 38.32221019989872 40.0 38.0 40.0 35.0 41.0 38-39 38.22036693679632 39.0 37.5 40.0 34.5 41.0 40-41 38.083006113533486 39.0 37.0 40.0 34.0 41.0 42-43 37.97736723940531 39.0 37.0 40.0 34.0 41.0 44-45 37.836656919398905 39.0 37.0 40.0 34.0 41.0 46-47 37.683110674818046 39.0 37.0 40.0 33.0 41.0 48-49 37.54913938419591 39.0 36.0 40.0 33.0 41.0 50-51 37.36788465918012 39.0 36.0 40.0 33.0 41.0 52-53 37.17755296437221 38.0 35.5 40.0 33.0 41.0 54-55 36.95346684871734 38.0 35.0 40.0 33.0 41.0 56-57 36.67161829255738 38.0 35.0 40.0 32.5 40.5 58-59 36.371427576102 37.5 35.0 39.5 32.0 40.0 60-61 35.990294934575104 37.0 34.5 39.0 31.5 40.0 62-63 35.670607776115105 36.0 34.0 39.0 31.0 40.0 64-65 35.34007712889583 36.0 34.0 38.5 31.0 40.0 66-67 34.979319899296726 35.0 34.0 38.0 31.0 40.0 68-69 34.614306125908215 35.0 33.5 37.0 30.5 39.0 70-71 34.24386722848642 35.0 33.0 37.0 30.0 39.0 72-73 33.88937197715978 35.0 33.0 36.0 30.0 38.5 74-75 33.52924232324671 34.0 33.0 35.5 29.5 37.5 76-77 33.19433424462662 34.0 33.0 35.0 29.0 37.0 78-79 32.87580474752947 34.0 32.0 35.0 29.0 36.0 80-81 32.51344634201887 34.0 32.0 35.0 29.0 36.0 82-83 32.310713658064316 34.0 32.0 35.0 28.5 35.0 84-85 32.03251122824933 34.0 31.0 35.0 27.5 35.0 86-87 31.822205000431556 34.0 31.0 34.5 27.0 35.0 88-89 31.56667744689524 33.0 31.0 34.0 27.0 35.0 90-91 31.334087082755758 33.0 31.0 34.0 27.0 35.0 92-93 31.064350165395048 33.0 30.0 34.0 26.0 35.0 94-95 30.76231571788523 33.0 30.0 34.0 25.0 35.0 96-97 30.396372539742128 33.0 30.0 34.0 24.5 35.0 98-99 29.89139716961806 32.0 29.5 34.0 24.0 35.0 100-101 27.76876201726847 30.5 26.5 33.0 19.5 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.9458641939897916 1101 2 1.4613109909152229 1101 3 2.5711276149178737 1101 4 1.6779788785648577 1101 5 0.5969227535681938 1101 6 0.5921972794804944 1101 7 0.00913105470952047 1101 8 0.3098500191958351 1101 9 0.46754505507377786 1101 10-11 0.43996225976549397 1101 12-13 0.3136200614398277 1101 14-15 0.22748482614274224 1101 16-17 0.18346438775090235 1101 18-19 0.09633252192318054 1101 20-21 0.08562575014399698 1101 22-23 0.08775639088869269 1101 24-25 0.05716604746809395 1101 26-27 0.06162028620596516 1101 28-29 0.03692709635446079 1101 30-31 0.06154131343414093 1101 32-33 0.055218800820497904 1101 34-35 0.0014415404971401813 1101 36-37 -0.019572112344327763 1101 38-39 -1.7540246007285987E-4 1101 40-41 0.0053979367552514645 1101 42-43 -0.019618513709851015 1101 44-45 0.05114435096649572 1101 46-47 0.12510418027356707 1101 48-49 0.036345586181752765 1101 50-51 0.010488293383566827 1101 52-53 0.04319895905442195 1101 54-55 -0.024956494661054762 1101 56-57 -0.04280638734323361 1101 58-59 -0.029652850800005126 1101 60-61 0.012131869339306434 1101 62-63 0.058789587705120994 1101 64-65 0.020524628970164827 1101 66-67 0.010543502900823398 1101 68-69 0.034467644187763824 1101 70-71 -0.001696992895205085 1101 72-73 -0.0417847579256545 1101 74-75 -0.05220982811918873 1101 76-77 -0.02571959042842309 1101 78-79 -0.0972604460139408 1101 80-81 -0.08474983541091063 1101 82-83 -0.04287442893890159 1101 84-85 -0.08493418452879808 1101 86-87 -0.05791490795765242 1101 88-89 -0.018566024960815497 1101 90-91 -0.12567068720258945 1101 92-93 0.024743165096872133 1101 94-95 -0.024576811359022344 1101 96-97 -0.04492312825059486 1101 98-99 -0.12753364377633858 1101 100-101 -0.06519500811075218 1102 1 0.5501159980010399 1102 2 0.7355995276127771 1102 3 -0.9673835451229174 1102 4 0.9941195610334859 1102 5 1.0732144941165132 1102 6 0.7759870700256499 1102 7 0.7969183074458712 1102 8 0.5338312790897746 1102 9 0.6119909855160941 1102 10-11 0.6114640780954659 1102 12-13 0.4174387610401382 1102 14-15 0.153050135158459 1102 16-17 0.027157020606495053 1102 18-19 0.09608851320086131 1102 20-21 0.0792357625023854 1102 22-23 -2.556244467655233E-5 1102 24-25 0.016437257036898245 1102 26-27 -0.02519345586928523 1102 28-29 -0.014225354036327076 1102 30-31 -0.008497234925052055 1102 32-33 -0.02793576992196023 1102 34-35 -0.015565778878837477 1102 36-37 -0.004542326452018131 1102 38-39 0.0985309232261784 1102 40-41 -0.010768416080480847 1102 42-43 0.0026344765410115656 1102 44-45 0.005211222051357822 1102 46-47 -0.010915478002033296 1102 48-49 -0.09174578143030487 1102 50-51 -0.1314876576599815 1102 52-53 -0.1355993548202079 1102 54-55 -0.11370820921720792 1102 56-57 -0.03817494532106025 1102 58-59 0.04266997643001247 1102 60-61 0.006609123829335317 1102 62-63 -0.027305856461609324 1102 64-65 -0.07084719013331409 1102 66-67 -0.11610942369816257 1102 68-69 -0.1419574523676559 1102 70-71 -0.20867897846784444 1102 72-73 -0.11878338569027136 1102 74-75 -0.13642243426224354 1102 76-77 -0.1561767945755932 1102 78-79 -0.05522245209925103 1102 80-81 -0.1845028300902527 1102 82-83 -0.11846081747400916 1102 84-85 0.03686742648971375 1102 86-87 -0.05427492666350986 1102 88-89 -0.011715181160056432 1102 90-91 -0.030839273168353998 1102 92-93 -0.0821100700779347 1102 94-95 -0.07350476723020094 1102 96-97 -0.15350413117515416 1102 98-99 -0.07458633430832862 1102 100-101 -0.15802231783856868 1103 1 0.1345798885955567 1103 2 0.6310926189208672 1103 3 1.0779271552654812 1103 4 1.9150774403575497 1103 5 1.3519982882109716 1103 6 0.8492095760478264 1103 7 0.6971392111892456 1103 8 0.515379639933137 1103 9 0.5295662762697404 1103 10-11 0.42001265330265625 1103 12-13 0.16970547687726167 1103 14-15 0.018005918076930527 1103 16-17 -0.007820616522778323 1103 18-19 -0.0664514983529827 1103 20-21 0.01021696751777057 1103 22-23 0.03583042139447201 1103 24-25 -0.03187181200642897 1103 26-27 0.017106613526465253 1103 28-29 -0.04267851917278165 1103 30-31 -0.02589840048295855 1103 32-33 -0.010034119320465607 1103 34-35 -0.05575354706070357 1103 36-37 -0.03370261973080346 1103 38-39 -0.10320724043879181 1103 40-41 -0.02916737260095914 1103 42-43 -0.08091518865175118 1103 44-45 -0.049825328798277724 1103 46-47 -0.13533324404026104 1103 48-49 -0.039285229824933765 1103 50-51 -0.06674932686389212 1103 52-53 -0.13587003457154623 1103 54-55 -0.2888570830804369 1103 56-57 -0.14814313590867556 1103 58-59 -0.11641069340245735 1103 60-61 -0.24474018748144744 1103 62-63 -0.09000818503412944 1103 64-65 -0.26688382091523977 1103 66-67 -0.2503297933485982 1103 68-69 -0.22742412969196124 1103 70-71 -0.17617379195314697 1103 72-73 -0.12153014934143158 1103 74-75 -0.14647119295034372 1103 76-77 -0.13025602926038715 1103 78-79 -0.09003477474951183 1103 80-81 -0.14989715053874164 1103 82-83 -0.24419701189836474 1103 84-85 -0.18147030917425155 1103 86-87 -0.20390587571395002 1103 88-89 -0.38177800066207723 1103 90-91 -0.2795807939888917 1103 92-93 -0.3691196219848685 1103 94-95 -0.2846286150246158 1103 96-97 -0.2974344556638613 1103 98-99 -0.2238049054825737 1103 100-101 -0.38639427904961465 1104 1 0.0653875971216138 1104 2 -0.22342537755534764 1104 3 0.9345510875878773 1104 4 0.002745907053196106 1104 5 0.010552702585926 1104 6 0.2379439224967399 1104 7 0.21920005369433682 1104 8 0.17182648405302103 1104 9 0.2312690851648469 1104 10-11 0.1465074019469128 1104 12-13 0.05909031806175591 1104 14-15 -0.004719720241460834 1104 16-17 -0.04164572986903181 1104 18-19 0.01319488094101473 1104 20-21 0.03351975647308336 1104 22-23 0.00849145970175158 1104 24-25 -0.12759914030360164 1104 26-27 -0.04600097797592184 1104 28-29 0.05327926077694656 1104 30-31 0.04241784782951186 1104 32-33 -0.0027577309718438414 1104 34-35 -0.0018703549947787224 1104 36-37 -0.06341045279881996 1104 38-39 0.06261232572371966 1104 40-41 0.07181294584727027 1104 42-43 0.07903460230735249 1104 44-45 -0.01997959248073755 1104 46-47 -0.049742519637554494 1104 48-49 0.029817782063751963 1104 50-51 0.05572367270135459 1104 52-53 0.09453217711055117 1104 54-55 0.09209890719155567 1104 56-57 0.026690195245450354 1104 58-59 0.051296615020298475 1104 60-61 -0.05868970385985506 1104 62-63 -0.01843823723714877 1104 64-65 -0.01356372356983826 1104 66-67 -0.11882750190734725 1104 68-69 -0.13143856479121752 1104 70-71 -0.07697579639219754 1104 72-73 0.0010372373368241483 1104 74-75 -0.06199676981495372 1104 76-77 -0.07539035315446085 1104 78-79 -0.017147901994782444 1104 80-81 -0.16409076588388416 1104 82-83 -0.06335007755910738 1104 84-85 -0.07904435606871374 1104 86-87 -0.03758475032537234 1104 88-89 -0.1927612692183409 1104 90-91 -0.052177518306606885 1104 92-93 0.020543681613123255 1104 94-95 -0.1654753042025412 1104 96-97 -0.17265979978865076 1104 98-99 -0.10720883165959094 1104 100-101 -0.4930602974007563 1105 1 -1.1224145062255424 1105 2 -0.6965621962427768 1105 3 -1.5945570123211112 1105 4 -0.2817545479978705 1105 5 -0.3571614242216441 1105 6 -0.452990449027169 1105 7 -0.4691278864411643 1105 8 -0.2928590250985579 1105 9 -0.629912530772053 1105 10-11 -0.27018791608322346 1105 12-13 -0.344562730780396 1105 14-15 -0.3369550322042443 1105 16-17 -0.3194430298993751 1105 18-19 -0.23112962103087398 1105 20-21 -0.050559776341145835 1105 22-23 -0.08711350541100416 1105 24-25 -0.11916047107516192 1105 26-27 -0.16815058865444854 1105 28-29 -0.1112621488701393 1105 30-31 -0.031755476065718824 1105 32-33 -0.02895603211452169 1105 34-35 -0.05469672773216416 1105 36-37 -0.1111731183868443 1105 38-39 -0.03586451723310802 1105 40-41 0.02315787455594176 1105 42-43 0.05649693419126578 1105 44-45 0.07703603093922595 1105 46-47 0.08274559847347263 1105 48-49 0.06781581017579441 1105 50-51 0.0528126099043007 1105 52-53 0.06164968140825522 1105 54-55 0.1004427741143985 1105 56-57 0.11337110332513589 1105 58-59 0.11964149316774098 1105 60-61 0.16632040838607054 1105 62-63 0.09317062109028029 1105 64-65 0.21701191602971193 1105 66-67 0.20700043822815672 1105 68-69 0.11239802131455434 1105 70-71 0.18683753154793692 1105 72-73 0.18310948933399374 1105 74-75 0.231051061108289 1105 76-77 0.22877968426084294 1105 78-79 0.18935174407661037 1105 80-81 0.08269353991043005 1105 82-83 0.0879569302273211 1105 84-85 0.23065834390094864 1105 86-87 0.18295322115809398 1105 88-89 -0.005187955435349778 1105 90-91 0.08916513514672175 1105 92-93 0.09427333071818467 1105 94-95 -0.02529429500039626 1105 96-97 0.0332849367873429 1105 98-99 0.17931028048521824 1105 100-101 0.04512101516876399 1106 1 0.0859343667926673 1106 2 0.29697767949132725 1106 3 1.0938636656476142 1106 4 0.9028863111855046 1106 5 0.8434405612471423 1106 6 0.6937825838808536 1106 7 0.5747002149833307 1106 8 0.3980779211792651 1106 9 0.4689608743146536 1106 10-11 0.3995313637007385 1106 12-13 0.21086635796216768 1106 14-15 0.029031241289047216 1106 16-17 -0.035152297225792495 1106 18-19 -0.10989570557191541 1106 20-21 -0.14572493546494059 1106 22-23 -0.12302720019354041 1106 24-25 -0.1416486518874578 1106 26-27 -0.0823653380721936 1106 28-29 -0.11111474754390827 1106 30-31 -0.07950422063972695 1106 32-33 -0.10187829242295265 1106 34-35 -0.06181609572479374 1106 36-37 -0.03763378428491393 1106 38-39 -0.04336767030608968 1106 40-41 -0.10708880020941791 1106 42-43 -0.12408013619770486 1106 44-45 -0.20856622534563485 1106 46-47 -0.16932072825452593 1106 48-49 -0.19651771143919206 1106 50-51 -0.12867130748904998 1106 52-53 -0.30862515822993686 1106 54-55 -0.22173048627912806 1106 56-57 -0.144991697377435 1106 58-59 -0.17634655997411386 1106 60-61 -0.28084646848546413 1106 62-63 -0.25911738794722794 1106 64-65 -0.328225202796645 1106 66-67 -0.37531411078568766 1106 68-69 -0.34794173680798934 1106 70-71 -0.3976257496597526 1106 72-73 -0.37996860628729934 1106 74-75 -0.41866999832423346 1106 76-77 -0.561400911379792 1106 78-79 -0.39546011612038967 1106 80-81 -0.3687073530088867 1106 82-83 -0.30739044943361904 1106 84-85 -0.3477638042122351 1106 86-87 -0.5311435298226428 1106 88-89 -0.40779784873381075 1106 90-91 -0.3089182607322307 1106 92-93 -0.46903611921565513 1106 94-95 -0.5345801916724504 1106 96-97 -0.5262992444582579 1106 98-99 -0.5318303194884741 1106 100-101 -0.668373363463072 1107 1 -2.370178358541711 1107 2 -0.6500131696989193 1107 3 -1.7002706018702938 1107 4 -3.404301798437256 1107 5 -1.7771757468468117 1107 6 -0.31443500574934546 1107 7 -0.23663630624002963 1107 8 0.06565013556839716 1107 9 0.3529997610829767 1107 10-11 0.3251729386237585 1107 12-13 0.19622261413142184 1107 14-15 0.05407539286108687 1107 16-17 0.029839540158420164 1107 18-19 -0.16190236252727885 1107 20-21 -0.1413932962314135 1107 22-23 -0.11211316527957393 1107 24-25 -0.13197575744727885 1107 26-27 -0.11062879859841246 1107 28-29 -0.09196420487184298 1107 30-31 0.0021692324416449082 1107 32-33 -4.848053779653583E-4 1107 34-35 -0.05986474647278328 1107 36-37 -0.06249437437470107 1107 38-39 -0.08782905451212741 1107 40-41 -0.07336573489402554 1107 42-43 -0.0747665860329434 1107 44-45 -0.08242042667235694 1107 46-47 -0.022538312320563136 1107 48-49 0.02781845701671415 1107 50-51 -0.11025517767063064 1107 52-53 -0.1897127614032783 1107 54-55 -0.15526036412366295 1107 56-57 -0.12947655119039325 1107 58-59 -0.15478111827477647 1107 60-61 -0.1706768619046244 1107 62-63 -0.11190302663992213 1107 64-65 -0.12762705610548153 1107 66-67 -0.08540832306266566 1107 68-69 -0.16221073478152448 1107 70-71 -0.24084360080523481 1107 72-73 -0.24997775375855014 1107 74-75 -0.18262395382657104 1107 76-77 -0.15700851157075135 1107 78-79 -0.12132219851662995 1107 80-81 -0.015174392622334665 1107 82-83 -0.08109469677589942 1107 84-85 -0.08500291488217471 1107 86-87 -0.1045439186458168 1107 88-89 -0.30091231787444883 1107 90-91 -0.26448336190526334 1107 92-93 -0.290468744528777 1107 94-95 -0.3498885182082425 1107 96-97 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34-35 -0.08638219706498518 2214 36-37 -0.1467622870542371 2214 38-39 -0.1806069013225553 2214 40-41 -0.10745654894434864 2214 42-43 -0.03190432688887057 2214 44-45 -0.012047692154823153 2214 46-47 -1.974203098100702E-4 2214 48-49 0.17652768908551764 2214 50-51 0.14976811487634478 2214 52-53 0.01663626477827762 2214 54-55 0.11306063692417467 2214 56-57 0.03821201449292033 2214 58-59 -0.056018538566952714 2214 60-61 0.04100991516915542 2214 62-63 -0.02107239852374221 2214 64-65 0.07849704973909866 2214 66-67 0.04970831291765876 2214 68-69 -0.04497302534873171 2214 70-71 -0.0751342146077647 2214 72-73 -0.020113201801059688 2214 74-75 0.021044248949422695 2214 76-77 -0.14401674849401047 2214 78-79 -0.013322651344289227 2214 80-81 0.10297300457443015 2214 82-83 -0.011572656972468565 2214 84-85 -0.02621238292316974 2214 86-87 9.186286830384915E-4 2214 88-89 0.06597875138428577 2214 90-91 0.25418054557929537 2214 92-93 0.28950731176044897 2214 94-95 0.43741017360967405 2214 96-97 0.13130276470556623 2214 98-99 0.24586258041958686 2214 100-101 0.3808096662782354 2215 1 -1.089994379662798 2215 2 0.08798788864686813 2215 3 -0.3274478905430591 2215 4 -3.512106454156225 2215 5 -1.352770314482484 2215 6 -0.20304446970937562 2215 7 -0.010183604916235822 2215 8 0.09773365895762254 2215 9 0.009360505190286972 2215 10-11 0.11549274451254377 2215 12-13 0.08483995481923756 2215 14-15 -0.054507895300403675 2215 16-17 -0.0697980229538615 2215 18-19 -0.19337302279040358 2215 20-21 -0.24028427086190618 2215 22-23 -0.16308973631083035 2215 24-25 -0.25754216578113187 2215 26-27 -0.2538119561494341 2215 28-29 -0.15499574973446784 2215 30-31 -0.13128858302667368 2215 32-33 -0.15434961137058423 2215 34-35 -0.20070305586921933 2215 36-37 -0.20538904677085412 2215 38-39 -0.12534206006378668 2215 40-41 -0.1379356583966782 2215 42-43 -0.13217029840635774 2215 44-45 -0.07894097168463077 2215 46-47 -0.00518077905392289 2215 48-49 -0.06151742026100493 2215 50-51 -0.1413763081386037 2215 52-53 -0.37791660968604646 2215 54-55 -0.38619868290057013 2215 56-57 -0.36069896921692646 2215 58-59 -0.3351239027266857 2215 60-61 -0.1678168041467245 2215 62-63 -0.18215350300293665 2215 64-65 -0.17075625807122208 2215 66-67 -0.14844407481398036 2215 68-69 -0.2804558141538749 2215 70-71 -0.27420583934262766 2215 72-73 -0.28450897030484157 2215 74-75 -0.1937560078838345 2215 76-77 -0.025194694501308845 2215 78-79 -0.23560966972181774 2215 80-81 -0.16693217393429194 2215 82-83 -0.3434406619937036 2215 84-85 -0.366312639275467 2215 86-87 -0.1657927272907358 2215 88-89 -0.06869698460651819 2215 90-91 -0.12605712137434466 2215 92-93 -0.25069502859652104 2215 94-95 -0.15008207089173808 2215 96-97 -0.14880720706201345 2215 98-99 -0.2060809835987456 2215 100-101 -0.2812333605064943 2216 1 1.3671166033920343 2216 2 0.6452999544654006 2216 3 0.7721608945364693 2216 4 2.2435214397323904 2216 5 1.5407259450325412 2216 6 1.0735678613023367 2216 7 0.8566783326141376 2216 8 0.6333766503223472 2216 9 0.6204258017744664 2216 10-11 0.5340147873213468 2216 12-13 0.41821704570075013 2216 14-15 0.3671775938439126 2216 16-17 0.24510945563748265 2216 18-19 0.055474751207022166 2216 20-21 0.0790285269418689 2216 22-23 0.05418926675309166 2216 24-25 0.1773687618025903 2216 26-27 0.06928839404559284 2216 28-29 0.05566599005666717 2216 30-31 0.0437478367172659 2216 32-33 0.059066553879461026 2216 34-35 0.0741710345908757 2216 36-37 -0.0032784855836709426 2216 38-39 0.006936390981842067 2216 40-41 0.01026201307345076 2216 42-43 -6.133981102820485E-4 2216 44-45 0.006362629404499387 2216 46-47 0.06642424108128608 2216 48-49 0.07354339375505958 2216 50-51 -0.02893006821349786 2216 52-53 -0.08321928401275613 2216 54-55 -0.05550639809682423 2216 56-57 -0.09622879683013963 2216 58-59 0.030641235028802782 2216 60-61 0.06733575486200749 2216 62-63 0.06580060722210135 2216 64-65 0.11240657790258979 2216 66-67 0.10789981598153986 2216 68-69 0.12262660217832178 2216 70-71 0.0957891386372296 2216 72-73 0.06857068593586035 2216 74-75 0.07446816944059265 2216 76-77 0.10279703150732189 2216 78-79 -0.044136007502267205 2216 80-81 0.06976457772972111 2216 82-83 0.14310967018219145 2216 84-85 0.20432096329020766 2216 86-87 0.2062975079543321 2216 88-89 0.3410517819916201 2216 90-91 0.4095460955010779 2216 92-93 0.2923824824023278 2216 94-95 0.2024947101414547 2216 96-97 0.30893057482074227 2216 98-99 0.2086471114848365 2216 100-101 0.4928243150835705 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 10.0 13 50.0 14 186.0 15 395.0 16 659.0 17 1137.0 18 1983.0 19 3246.0 20 3219.0 21 3201.0 22 3458.0 23 4122.0 24 4719.0 25 6158.0 26 8391.0 27 11368.0 28 15319.0 29 21670.0 30 30595.0 31 44980.0 32 69655.0 33 116666.0 34 214300.0 35 409448.0 36 603938.0 37 323897.0 38 20485.0 39 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.776442864165674 33.52901854804519 12.22001711486991 25.474521472919232 2 27.006126541709413 26.20207968611808 26.412034497478516 20.379759274694 3 25.636082727806464 24.246261403518808 21.684197607776674 28.433458260898053 4 25.79181379474223 25.27372191610801 22.509282652813447 26.425181636336315 5 25.369396144283137 26.211865807991998 23.881464627504972 24.537273420219897 6 25.26183230357833 24.133339364718374 24.43891220851122 26.16591612319208 7 26.422028270542835 24.788823641353233 23.406441307894767 25.382706780209162 8 25.479728837388745 23.88427233977062 24.956818425247782 25.679180397592855 9 24.33880975877592 23.517969878446856 25.541966459277255 26.601253903499973 10-11 25.807503247067242 24.412330224398605 24.252446609271484 25.527719919262676 12-13 25.78270178872069 24.489204346338585 24.1617679020254 25.56632596291532 14-15 25.45552531776544 24.52461271768869 24.31983170364701 25.700030260898863 16-17 25.680818229747814 24.80210827994347 24.281381644009123 25.23569184629959 18-19 24.98146389957957 24.161637915346436 24.955206590428613 25.90169159464538 20-21 24.886573623935018 24.744628170505088 24.75627497694036 25.612523228619533 22-23 24.884233863713646 24.870845235780237 25.397265288253262 24.847655612252854 24-25 24.840220374216052 24.17172488163413 25.953400295537715 25.034654448612102 26-27 24.70441029203327 24.154150682638043 26.36410620639597 24.777332818932717 28-29 24.840480347573983 24.38690482999302 26.140373134561166 24.63224168787183 30-31 24.82568786350775 24.25930990592084 26.07436589898267 24.84063633158874 32-33 24.73794685520628 24.247117155433912 26.206068402110148 24.808867587249658 34-35 24.90656557515986 24.358281763284896 26.016521826843185 24.71863083471206 36-37 25.001403856132825 24.28634713514559 25.78173988729635 24.93050912142524 38-39 25.005979387232397 24.33363628895311 25.80339566801194 24.856988655802553 40-41 25.265536787789987 24.562438841267547 25.186660870993943 24.985363499948527 42-43 25.418219140902444 24.44945441991105 25.015884372169538 25.11644206701697 44-45 25.32348484927265 24.545332594315735 25.101285620249637 25.029896936161983 46-47 25.25209616518499 24.89026524561763 24.80260222932354 25.05503635987384 48-49 25.423288621382078 24.757834817087947 24.612561704676505 25.206314856853467 50-51 25.208160667694774 24.842066185057355 24.786327897117104 25.163445250130767 52-53 25.32351084660844 25.008917086177007 24.59420758560663 25.073364481607925 54-55 25.45534333641488 24.82415402069596 24.476725625157933 25.243777017731222 56-57 25.226358802749893 24.92229396331464 24.692399522896892 25.15894771103857 58-59 25.311552072143645 25.00535545117336 24.52167501874408 25.16141745793891 60-61 25.410523929507704 24.84185820637101 24.460269311600946 25.28734855252034 62-63 25.26795454001874 24.848747500356165 24.61131383255844 25.27198412706666 64-65 25.30996623466027 25.04913496464882 24.541017036574093 25.099881764116816 66-67 25.46381846788341 24.84123427031198 24.451456214767113 25.243491047037498 68-69 25.33177799939062 24.954114702325306 24.478025491947587 25.236081806336486 70-71 25.392949730511617 25.07588622317985 24.35872371799338 25.172440328315155 72-73 25.501696586133853 24.873652948045883 24.242229656304822 25.382420809515445 74-75 25.362740826320117 24.87952834593511 24.52349483224959 25.23423599549518 76-77 25.463090542481208 25.065019336818363 24.275324264769345 25.196565855931084 78-79 25.49241553725574 24.841806211699424 24.321781503831488 25.343996747213343 80-81 25.33131004734635 24.867257603440798 24.472072102050983 25.32936024716187 82-83 25.411225857574117 25.10242950302453 24.36714685479032 25.119197784611032 84-85 25.493637412038012 24.965189567373137 24.230842823227476 25.310330197361374 86-87 25.299281329649336 25.03821608361575 24.346218999476935 25.316283587257978 88-89 25.391493879707205 25.105757162006036 24.328982765846156 25.1737661924406 90-91 25.456019267145503 24.959834116199772 24.218572080733168 25.36557453592156 92-93 25.279107397073947 25.06361548068554 24.30301142738892 25.35426569485159 94-95 25.457553109957292 25.207510734299948 24.192548747604345 25.14238740813841 96-97 25.43272565427495 25.13916373850008 24.07693859533275 25.35117201189222 98-99 25.355617556312833 25.097827974589165 24.245089363242055 25.301465105855954 100-101 25.316569557951702 25.234106008816216 24.12794536815867 25.321379065073412 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 456.0 1 307.0 2 147.0 3 121.0 4 94.0 5 75.5 6 66.5 7 48.0 8 36.0 9 35.0 10 35.0 11 31.0 12 25.0 13 34.5 14 39.5 15 52.5 16 85.0 17 79.0 18 77.0 19 116.5 20 157.0 21 230.5 22 345.5 23 521.0 24 736.0 25 1053.5 26 1526.5 27 2180.0 28 2955.0 29 3953.0 30 5260.0 31 6888.0 32 8783.0 33 11144.5 34 13881.5 35 16949.5 36 20785.0 37 24664.5 38 28495.5 39 32766.5 40 37135.5 41 41165.5 42 45773.0 43 51169.5 44 57269.0 45 63205.5 46 68998.0 47 75172.5 48 80341.0 49 83842.5 50 85570.5 51 87279.0 52 89915.0 53 92014.0 54 91391.0 55 89070.5 56 83864.5 57 76414.0 58 72787.0 59 69303.0 60 59580.5 61 49350.0 62 40992.0 63 31936.5 64 25032.5 65 20089.5 66 14944.5 67 10471.0 68 7824.0 69 6149.0 70 5296.0 71 5020.5 72 4814.0 73 4013.0 74 2986.0 75 2543.5 76 2082.5 77 1436.0 78 931.0 79 521.0 80 219.5 81 178.0 82 127.5 83 42.0 84 4.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05038283676688814 2 1.5598401475816758E-4 3 0.015234438774714367 4 0.003275664309921519 5 0.0 6 5.199467158605586E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1923274.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.49818572337234 #Duplication Level Percentage of deduplicated Percentage of total 1 89.88392422638893 66.9618928056305 2 5.510286358860841 8.210126731027604 3 1.7165276528565094 3.83634587645426 4 0.9034023029917724 2.692073302048134 5 0.5382606931955229 2.004972254463561 6 0.35162488397457115 1.5717249546778111 7 0.23635671544889802 1.2325702539134793 8 0.17323522526197158 1.0324567988317284 9 0.11992610474101048 0.8040849501668763 >10 0.48547317303068777 6.519807696093531 >50 0.0652491535850498 3.359404372683138 >100 0.015452810337944153 1.5633329395367004 >500 7.017483157069777E-5 0.028701158018676582 >1k 2.1052449471209124E-4 0.18250590645391435 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 5.199467158605586E-5 0.0 0.0 0.0 0.0 5 5.199467158605586E-5 0.0 0.0 0.0 0.0 6 5.199467158605586E-5 0.0 0.0 0.0 0.0 7 5.199467158605586E-5 0.0 0.0 0.0 0.0 8 5.199467158605586E-5 0.0 0.0 0.0 0.0 9 2.0797868634422345E-4 0.0 0.0 0.0 5.199467158605586E-5 10-11 2.599733579302793E-4 0.0 0.0 0.0 5.199467158605586E-5 12-13 3.63962701102391E-4 2.599733579302793E-5 0.0 0.0 5.199467158605586E-5 14-15 0.0010658907675141452 5.199467158605586E-5 0.0 0.0 1.0398934317211172E-4 16-17 0.0019498001844770949 5.199467158605586E-5 0.0 0.0 1.819813505511955E-4 18-19 0.003197672302542435 5.199467158605586E-5 0.0 0.0 2.339760221372514E-4 20-21 0.005251461830191642 5.199467158605586E-5 0.0 0.0 2.599733579302793E-4 22-23 0.007877192745287462 5.199467158605586E-5 0.0 0.0 3.1196802951633516E-4 24-25 0.010372936981418144 5.199467158605586E-5 0.0 0.0 3.1196802951633516E-4 26-27 0.012114758479551015 5.199467158605586E-5 0.0 0.0 3.3796536530936313E-4 28-29 0.013622603955546635 5.199467158605586E-5 0.0 0.0 3.6396270110239105E-4 30-31 0.015494412132644648 5.199467158605586E-5 0.0 0.0 3.8996003689541897E-4 32-33 0.017054252280226324 5.199467158605586E-5 0.0 5.199467158605586E-5 4.159573726884469E-4 34-35 0.018198135055119552 7.799200737908379E-5 0.0 5.199467158605586E-5 4.159573726884469E-4 36-37 0.020121937903803618 1.2998667896513966E-4 0.0 5.199467158605586E-5 4.159573726884469E-4 38-39 0.022799663490485497 1.5598401475816758E-4 0.0 5.199467158605586E-5 4.419547084814748E-4 40-41 0.02599733579302793 1.5598401475816758E-4 0.0 5.199467158605586E-5 4.6795204427450273E-4 42-43 0.030728850907359013 1.5598401475816758E-4 0.0 5.199467158605586E-5 4.6795204427450273E-4 44-45 0.03631827810286002 1.5598401475816758E-4 0.0 5.199467158605586E-5 4.6795204427450273E-4 46-47 0.044065484169182344 1.819813505511955E-4 0.0 5.199467158605586E-5 4.939493800675307E-4 48-49 0.054048461113705065 2.0797868634422345E-4 0.0 5.199467158605586E-5 5.199467158605586E-4 50-51 0.06540929685525827 2.0797868634422345E-4 0.0 5.199467158605586E-5 5.199467158605586E-4 52-53 0.07996780489935391 2.0797868634422345E-4 0.0 5.199467158605586E-5 5.199467158605586E-4 54-55 0.09876387867771311 2.0797868634422345E-4 0.0 5.199467158605586E-5 5.199467158605586E-4 56-57 0.12239545691357549 2.0797868634422345E-4 0.0 5.199467158605586E-5 5.199467158605586E-4 58-59 0.14927670212356636 2.0797868634422345E-4 0.0 5.199467158605586E-5 5.979387232396423E-4 60-61 0.1809674544552674 2.0797868634422345E-4 0.0 5.199467158605586E-5 6.499333948256983E-4 62-63 0.21855960201198582 2.0797868634422345E-4 0.0 5.199467158605586E-5 6.759307306187262E-4 64-65 0.26205314479372155 2.0797868634422345E-4 0.0 5.199467158605586E-5 6.759307306187262E-4 66-67 0.310850144077235 2.0797868634422345E-4 0.0 5.199467158605586E-5 6.759307306187262E-4 68-69 0.36843424285879184 2.0797868634422345E-4 0.0 5.199467158605586E-5 7.019280664117541E-4 70-71 0.4368592306660414 2.0797868634422345E-4 0.0 5.199467158605586E-5 7.279254022047821E-4 72-73 0.512797448517476 2.0797868634422345E-4 0.0 5.199467158605586E-5 7.279254022047821E-4 74-75 0.5964568750994399 2.0797868634422345E-4 0.0 5.199467158605586E-5 7.799200737908379E-4 76-77 0.6951167644339808 2.0797868634422345E-4 0.0 5.199467158605586E-5 7.799200737908379E-4 78-79 0.8053194708606262 2.0797868634422345E-4 0.0 5.199467158605586E-5 7.799200737908379E-4 80-81 0.9296387306228857 2.0797868634422345E-4 0.0 5.199467158605586E-5 7.799200737908379E-4 82-83 1.0690884398166878 2.0797868634422345E-4 0.0 5.199467158605586E-5 8.319147453768938E-4 84-85 1.2220047689512779 2.0797868634422345E-4 0.0 5.199467158605586E-5 8.319147453768938E-4 86-87 1.3855280110894235 2.0797868634422345E-4 0.0 5.199467158605586E-5 8.319147453768938E-4 88-89 1.5723188687623293 2.0797868634422345E-4 0.0 5.199467158605586E-5 8.839094169629496E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 1560 0.0 90.820595 1 TCGACGG 1660 0.0 85.584656 2 TATCTCG 665 0.0 67.87889 2 ACGGCGC 2320 0.0 67.56347 5 CGACGGC 2165 0.0 66.93831 3 GACGGCG 2400 0.0 64.720985 4 CGGCGCG 2705 0.0 57.77163 6 ATATCTC 975 0.0 44.37409 1 CGCGCCG 4845 0.0 39.215 9 ATCTCGA 1165 0.0 38.746323 3 GGCGCGC 5320 0.0 35.624374 7 GCGCGCC 5460 0.0 34.971912 8 TCTCGAG 1545 0.0 31.667759 4 GTTGACG 350 0.0 31.24298 1 CCATATT 3150 0.0 25.709866 18-19 ATCCATA 3155 0.0 25.368017 16-17 TCCATAT 3135 0.0 25.22683 18-19 TTGACGG 465 0.0 24.523989 2 CTCGAGG 2035 0.0 24.27476 5 TGACGGC 490 0.0 23.272764 3 >>END_MODULE