FastQCFastQC Report
Tue 31 May 2016
SRR2048262_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048262_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2450952
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT164460.6710045729169727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG69150.088.877091
TATCTCG63350.087.717642
ATCTCGA67500.082.535733
ATATCTC69300.080.529011
CTCGACG16700.080.4855045
TCGAGGG53550.078.49316
TCTCGAG55050.076.699434
CTCGAGG54850.076.6327445
AGGGCGC55200.076.497259
CGAGGGC57350.073.6234367
TCTCGAC18200.073.591124
TCGACGG84650.072.715292
GAGGGCG59450.070.7859958
CGACGGC88400.069.791833
ACGGCGC91300.067.5750055
GACGGCG91450.067.412234
CGGCGCG92700.066.708166
CGCGCCG144200.043.2167179
GGCGCGC145150.042.930367
GCGCGCC145250.042.869858
Produced by FastQC (version 0.11.5)