##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048262_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2450952 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.96118610237981 31.0 31.0 34.0 28.0 34.0 2 31.11923856525954 31.0 31.0 34.0 27.0 34.0 3 31.319869177364552 31.0 31.0 34.0 28.0 34.0 4 34.98501929046346 37.0 35.0 37.0 32.0 37.0 5 34.765681661656366 37.0 35.0 37.0 32.0 37.0 6 34.97271753995998 37.0 35.0 37.0 32.0 37.0 7 34.87009700720373 37.0 35.0 37.0 32.0 37.0 8 34.91655079332439 37.0 35.0 37.0 32.0 37.0 9 36.38835113865959 39.0 35.0 39.0 32.0 39.0 10-11 36.35070760259687 39.0 35.0 39.0 32.0 39.0 12-13 36.3611427314774 39.0 35.0 39.0 31.5 39.0 14-15 37.43241585310524 40.0 36.5 41.0 31.5 41.0 16-17 37.36206074211164 40.0 36.5 41.0 31.0 41.0 18-19 37.365241954962805 40.0 36.5 41.0 31.0 41.0 20-21 37.417233793236264 40.0 37.0 41.0 31.0 41.0 22-23 37.28490500833962 40.0 36.5 41.0 31.0 41.0 24-25 37.33219806018233 40.0 36.5 41.0 31.0 41.0 26-27 37.30330296146151 40.0 36.0 41.0 31.0 41.0 28-29 37.08615509402061 39.5 36.0 41.0 30.5 41.0 30-31 36.89710406405348 39.0 35.5 40.5 30.0 41.0 32-33 36.87404649295458 39.0 35.0 40.5 30.0 41.0 34-35 36.63585068169429 39.0 35.0 40.0 30.0 41.0 36-37 36.4800752931922 38.0 35.0 40.0 30.0 41.0 38-39 36.353425526081296 38.5 35.0 40.0 29.5 41.0 40-41 36.0290342283325 38.0 35.0 40.0 28.0 41.0 42-43 35.83290390835887 38.0 34.0 40.0 27.5 41.0 44-45 35.806776917703814 38.0 34.0 40.0 27.5 41.0 46-47 35.72765745718398 38.0 34.0 40.0 27.0 41.0 48-49 35.737446306578015 38.0 34.0 40.0 27.0 41.0 50-51 35.81447168283997 38.0 34.0 40.0 28.0 41.0 52-53 35.72187888624502 38.0 34.0 40.0 28.0 41.0 54-55 35.42916446344114 38.0 33.5 40.0 27.0 41.0 56-57 35.23758237615424 38.0 33.0 40.0 27.0 41.0 58-59 34.99874905750909 38.0 33.0 40.0 26.5 41.0 60-61 34.80283742806877 37.0 33.0 40.0 26.0 41.0 62-63 34.689999232951116 37.0 33.0 40.0 26.0 41.0 64-65 34.43407112827995 36.5 33.0 40.0 26.0 41.0 66-67 34.1883306976228 36.0 33.0 39.0 26.0 41.0 68-69 34.01642769829846 36.0 33.0 39.0 26.0 41.0 70-71 33.69044130607209 35.5 32.5 39.0 26.0 40.0 72-73 33.35597902366101 35.0 32.0 38.0 26.0 40.0 74-75 33.01969398013507 35.0 32.0 37.0 26.0 39.0 76-77 32.66657200957016 35.0 32.0 37.0 26.0 39.0 78-79 32.34447247436914 35.0 32.0 36.0 25.0 39.0 80-81 32.029314119574764 35.0 32.0 36.0 25.0 37.5 82-83 31.763793211780566 35.0 32.0 35.5 25.0 37.0 84-85 31.522281138104702 35.0 31.5 35.0 25.0 37.0 86-87 31.298894470393545 34.0 31.0 35.0 24.0 36.0 88-89 31.083243164288817 34.0 31.0 35.0 24.0 36.0 90-91 30.897074279708455 34.0 31.0 35.0 23.0 36.0 92-93 30.71037723300987 34.0 31.0 35.0 23.0 35.0 94-95 30.525536403813703 34.0 31.0 35.0 20.0 35.0 96-97 30.339454832244776 34.0 31.0 35.0 19.5 35.0 98-99 30.081440599407905 34.0 31.0 35.0 16.5 35.0 100-101 29.328518265555587 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8573873810231127 1101 2 0.9193283579684106 1101 3 0.8685355007392381 1101 4 0.7486710182435417 1101 5 0.8763227186853655 1101 6 0.6348373016198536 1101 7 0.6532456324025517 1101 8 0.7421423902689028 1101 9 0.8796303659314901 1101 10-11 0.8864152670771901 1101 12-13 0.8835666302158103 1101 14-15 1.0233729424661462 1101 16-17 1.0202594285294495 1101 18-19 1.115306852000728 1101 20-21 1.1396752869716167 1101 22-23 1.1633040058197395 1101 24-25 1.0121977896367795 1101 26-27 0.9569065087508193 1101 28-29 1.194213393545418 1101 30-31 1.1651555448345405 1101 32-33 1.0447466743737053 1101 34-35 1.1132432542743373 1101 36-37 1.1798215914825008 1101 38-39 1.1753284982768335 1101 40-41 1.1464725442311945 1101 42-43 1.047043239172794 1101 44-45 1.0579483894075423 1101 46-47 1.2025559646356498 1101 48-49 1.1582826327000646 1101 50-51 0.978641834929519 1101 52-53 1.00144282010055 1101 54-55 1.0270137221892242 1101 56-57 0.8212278537634106 1101 58-59 0.817180462436454 1101 60-61 0.7359374277281603 1101 62-63 0.6934192800412049 1101 64-65 0.6444749011831234 1101 66-67 0.6941902349365279 1101 68-69 0.6699367533036948 1101 70-71 0.5760974147179567 1101 72-73 0.6561036076024749 1101 74-75 0.5102709031488288 1101 76-77 0.5201250552148196 1101 78-79 0.434018943948729 1101 80-81 0.46230323894071645 1101 82-83 0.4735043797355942 1101 84-85 0.5020650598366743 1101 86-87 0.575606502280106 1101 88-89 0.60546336728423 1101 90-91 0.5897782494637482 1101 92-93 0.5573134301863902 1101 94-95 0.6164668959198671 1101 96-97 0.6446148867541837 1101 98-99 0.6766093776520492 1101 100-101 0.7717487333609974 1102 1 0.7267170975595505 1102 2 0.8193282356190004 1102 3 0.765280661597572 1102 4 0.7274557971759492 1102 5 0.7600988419530665 1102 6 0.6626917022612346 1102 7 0.7234581221863721 1102 8 0.7227220350640948 1102 9 0.870517837396271 1102 10-11 0.8935353354504869 1102 12-13 0.8993197008523524 1102 14-15 0.982782440829105 1102 16-17 1.0071862270991687 1102 18-19 1.0431793714776418 1102 20-21 0.9558677643959257 1102 22-23 1.1893190777100244 1102 24-25 1.05163817900155 1102 26-27 1.1356081957489934 1102 28-29 1.1361034294538612 1102 30-31 1.1156424621232262 1102 32-33 1.0864453558029794 1102 34-35 1.2161663874781397 1102 36-37 1.2330629110142226 1102 38-39 1.2068241008165401 1102 40-41 1.1614381295663492 1102 42-43 1.2399992443265972 1102 44-45 1.1361063889902212 1102 46-47 1.1408353581918789 1102 48-49 1.0311229709450203 1102 50-51 0.9611271830313939 1102 52-53 1.0025162971506916 1102 54-55 0.9989078935077629 1102 56-57 0.9724885142900632 1102 58-59 0.8890865461385715 1102 60-61 0.806771285680945 1102 62-63 0.6563753541555855 1102 64-65 0.6827266660177642 1102 66-67 0.7709190265559016 1102 68-69 0.8038017745413342 1102 70-71 0.7519670465263104 1102 72-73 0.6245543659686135 1102 74-75 0.6460972132552101 1102 76-77 0.6764686386110697 1102 78-79 0.7301950020820556 1102 80-81 0.7013936756213432 1102 82-83 0.7236338476580286 1102 84-85 0.7801278159640432 1102 86-87 0.6955340903372011 1102 88-89 0.7830527869075645 1102 90-91 0.8147970300983722 1102 92-93 0.7810935620657062 1102 94-95 0.8073144870379245 1102 96-97 0.8285112238797545 1102 98-99 0.9300271528414576 1102 100-101 0.923029498120453 1103 1 0.5590599538302428 1103 2 0.6154515487226888 1103 3 0.6460315739179627 1103 4 0.5280124088488307 1103 5 0.6008224651845211 1103 6 0.45158749185647906 1103 7 0.4428926535678741 1103 8 0.5090411515332676 1103 9 0.6681117715781255 1103 10-11 0.6704369891248447 1103 12-13 0.5745065389732602 1103 14-15 0.7434872020882963 1103 16-17 0.6703564558658144 1103 18-19 0.7120930073918643 1103 20-21 0.6712154977102855 1103 22-23 0.7651246058042886 1103 24-25 0.7324184330405714 1103 26-27 0.8603700250516795 1103 28-29 0.920133033334487 1103 30-31 0.9086594192686093 1103 32-33 0.7760304541378531 1103 34-35 0.8545308276719368 1103 36-37 0.9564511113226075 1103 38-39 0.968068196419928 1103 40-41 0.90012105843212 1103 42-43 0.9156123201501174 1103 44-45 0.7697074432944504 1103 46-47 0.8876790612705179 1103 48-49 0.7699126275767725 1103 50-51 0.7590958988898677 1103 52-53 0.6645240518038662 1103 54-55 0.6508980521079195 1103 56-57 0.6019868623664522 1103 58-59 0.6527181283995844 1103 60-61 0.5869450824768876 1103 62-63 0.5601176269138222 1103 64-65 0.5659045127452487 1103 66-67 0.5801317819043277 1103 68-69 0.5160707877673829 1103 70-71 0.43473545591561447 1103 72-73 0.4447340726322295 1103 74-75 0.5104653573850513 1103 76-77 0.5466273251794576 1103 78-79 0.4448512213985225 1103 80-81 0.49028999829428344 1103 82-83 0.48559894487288346 1103 84-85 0.5772578603239609 1103 86-87 0.5222987935459109 1103 88-89 0.6060725351355281 1103 90-91 0.6415769400497666 1103 92-93 0.5928400637573574 1103 94-95 0.5957570203084259 1103 96-97 0.6068882121613584 1103 98-99 0.766816831020769 1103 100-101 0.836012195765182 1104 1 0.5429356995744818 1104 2 0.5785853865089763 1104 3 0.5588510921600154 1104 4 0.49011009238078884 1104 5 0.5327810342519115 1104 6 0.4273727641248328 1104 7 0.5201835090584126 1104 8 0.568389440982422 1104 9 0.6777943462601073 1104 10-11 0.6387953501546875 1104 12-13 0.5629580911430949 1104 14-15 0.6984197579474127 1104 16-17 0.6999182795869316 1104 18-19 0.7545720266126992 1104 20-21 0.712165550772859 1104 22-23 0.8588392568999552 1104 24-25 0.7445922371608091 1104 26-27 0.6688811448870382 1104 28-29 0.7433652071789396 1104 30-31 0.7761808571746442 1104 32-33 0.745162905754114 1104 34-35 0.743210181402084 1104 36-37 0.8897277295517512 1104 38-39 0.892643830302525 1104 40-41 0.8467737356718672 1104 42-43 0.8123499916175092 1104 44-45 0.7511286529955754 1104 46-47 0.8990721643911712 1104 48-49 0.8822218667383837 1104 50-51 0.7079577425990848 1104 52-53 0.5969637245010233 1104 54-55 0.7206217211516446 1104 56-57 0.6331266701401219 1104 58-59 0.6494440131641781 1104 60-61 0.6481828296839254 1104 62-63 0.5738312651597397 1104 64-65 0.4535512415094587 1104 66-67 0.4192547727868856 1104 68-69 0.43261180056335036 1104 70-71 0.42021607552868545 1104 72-73 0.42461371157369854 1104 74-75 0.5199329255776277 1104 76-77 0.5313201291957199 1104 78-79 0.44248615001502856 1104 80-81 0.431918481421814 1104 82-83 0.4237851393722991 1104 84-85 0.4090788095524047 1104 86-87 0.46620574044031926 1104 88-89 0.40384587908137703 1104 90-91 0.4455525881839719 1104 92-93 0.41304024275390105 1104 94-95 0.3551419023159106 1104 96-97 0.37779173911929576 1104 98-99 0.42321192398782514 1104 100-101 0.5295992098662019 1105 1 0.369870714259406 1105 2 0.4064829691026368 1105 3 0.4207674138575328 1105 4 0.3624982961540155 1105 5 0.4575831291143615 1105 6 0.36738226773096017 1105 7 0.32253376396484157 1105 8 0.41372311159882713 1105 9 0.5182275786797916 1105 10-11 0.5324913611267803 1105 12-13 0.4699011660344041 1105 14-15 0.5396198459303676 1105 16-17 -1.6935539874707288 1105 18-19 0.08693914680302584 1105 20-21 0.448370175800882 1105 22-23 0.6091775289888304 1105 24-25 0.5564957185912505 1105 26-27 0.59366984563286 1105 28-29 0.6250518666839895 1105 30-31 0.6382118765923863 1105 32-33 0.5558433686999962 1105 34-35 0.6535185139668158 1105 36-37 0.6191911578488103 1105 38-39 0.6672895700465844 1105 40-41 0.7037082654558589 1105 42-43 0.5589445233365851 1105 44-45 0.5579454501898908 1105 46-47 0.629336413922772 1105 48-49 0.5678460687429094 1105 50-51 0.5417074803682809 1105 52-53 0.5849264827531613 1105 54-55 0.4709592585421376 1105 56-57 0.3874700656764105 1105 58-59 0.31066693218188135 1105 60-61 0.36645391937047833 1105 62-63 0.36660808933670097 1105 64-65 0.3522535155026816 1105 66-67 0.3613270551262744 1105 68-69 0.3730607385737912 1105 70-71 0.35491868318799646 1105 72-73 0.2777406387440706 1105 74-75 0.19316615682179616 1105 76-77 0.23016384393019962 1105 78-79 0.1888077412858138 1105 80-81 0.18839603446478392 1105 82-83 0.1037350114669664 1105 84-85 0.01711612160525533 1105 86-87 0.053192672303712385 1105 88-89 0.1646665168266992 1105 90-91 0.10394816177316812 1105 92-93 0.11473616963129274 1105 94-95 0.20368784379684612 1105 96-97 0.20661015310216513 1105 98-99 0.18513579612408648 1105 100-101 0.19932411505619996 1106 1 0.30203713055091086 1106 2 0.38852255582114736 1106 3 0.30487908037550326 1106 4 0.3410525526982511 1106 5 0.30600809654147554 1106 6 0.3444152124172817 1106 7 0.39460104753845116 1106 8 0.32899399846197497 1106 9 0.452280276996035 1106 10-11 0.45080119731678536 1106 12-13 0.43308232454857887 1106 14-15 0.3567854085005635 1106 16-17 0.4694307405056435 1106 18-19 0.4339946005665283 1106 20-21 0.504328249185555 1106 22-23 0.5620037139706611 1106 24-25 0.4145620243180943 1106 26-27 0.4514313493122444 1106 28-29 0.5655904356541654 1106 30-31 0.5773604356979689 1106 32-33 0.47334314565310365 1106 34-35 0.5238608097089639 1106 36-37 0.6442709294074476 1106 38-39 0.698071620105317 1106 40-41 0.6094695178989014 1106 42-43 0.4978339231951878 1106 44-45 0.4989735453717259 1106 46-47 0.604305299311811 1106 48-49 0.5061809516349669 1106 50-51 0.4481874203287646 1106 52-53 0.20258466605946523 1106 54-55 0.4151920524407302 1106 56-57 0.3593641190338204 1106 58-59 0.21411415205307094 1106 60-61 0.3911106194867173 1106 62-63 0.39020369481342243 1106 64-65 0.283743040451256 1106 66-67 0.33445777002113175 1106 68-69 0.26617999741279874 1106 70-71 0.16784865128626336 1106 72-73 0.2299419192456611 1106 74-75 0.2094057791129771 1106 76-77 0.314458431865269 1106 78-79 0.3192711127374892 1106 80-81 0.30508466708800697 1106 82-83 0.24992037566378755 1106 84-85 0.2275217959641438 1106 86-87 0.1687971305317113 1106 88-89 0.1817138444493409 1106 90-91 0.1269705625706976 1106 92-93 0.23843143142634204 1106 94-95 0.3437895394010475 1106 96-97 0.35197742033622603 1106 98-99 0.3581678823812844 1106 100-101 0.3296852638585044 1107 1 0.1839867779840354 1107 2 0.3515719457983195 1107 3 0.27182104180950617 1107 4 0.30463257392820964 1107 5 0.2617297356168464 1107 6 0.18905329340786636 1107 7 0.21635616354081577 1107 8 0.2516227455705291 1107 9 0.2213292207083839 1107 10-11 0.28038143167058394 1107 12-13 0.24114414576829546 1107 14-15 0.260996547105826 1107 16-17 0.24618867362832475 1107 18-19 0.34775080220522625 1107 20-21 0.3422309693404131 1107 22-23 0.3996346129234709 1107 24-25 0.3190797492152555 1107 26-27 0.3056927663031601 1107 28-29 0.4359728163180634 1107 30-31 0.4862466988070011 1107 32-33 0.3212302171019843 1107 34-35 0.3518630445655475 1107 36-37 0.5694402657150945 1107 38-39 0.5711837815283687 1107 40-41 0.324991085501253 1107 42-43 0.35295740255367036 1107 44-45 0.23216396209947732 1107 46-47 0.3893375593134749 1107 48-49 0.45780563820457587 1107 50-51 0.369001936063718 1107 52-53 0.3208459641249277 1107 54-55 0.45075138181890395 1107 56-57 0.3547862837950291 1107 58-59 0.3779310233362736 1107 60-61 0.32675198976701125 1107 62-63 0.2945268706176307 1107 64-65 0.36011372991865187 1107 66-67 0.3994986609494191 1107 68-69 0.43194135876779427 1107 70-71 0.28589694326355897 1107 72-73 0.20386930877892695 1107 74-75 0.1849735745706127 1107 76-77 0.2746908487072375 1107 78-79 0.3482663162736941 1107 80-81 0.29682102583782566 1107 82-83 0.31010157213628986 1107 84-85 0.3902013395494386 1107 86-87 0.35649402722493306 1107 88-89 0.34187905663413787 1107 90-91 0.380993392725717 1107 92-93 0.42186128866280725 1107 94-95 0.37458022294571336 1107 96-97 0.37803952179612565 1107 98-99 0.41729602926952936 1107 100-101 0.38947954048264677 1108 1 -0.005633433434660162 1108 2 0.067425074248181 1108 3 0.04089701104425103 1108 4 0.02173648821130314 1108 5 0.21046624914227152 1108 6 0.06585125257545599 1108 7 0.044873316681453446 1108 8 0.1431655734062076 1108 9 0.10632979360127592 1108 10-11 0.07818770657549834 1108 12-13 -0.10971349610756675 1108 14-15 -0.021321886768696174 1108 16-17 0.09053958598298095 1108 18-19 0.21421025745380717 1108 20-21 0.02175147314667214 1108 22-23 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2215 32-33 -0.95924485503744 2215 34-35 -1.8519252200707896 2215 36-37 -0.9852071188009788 2215 38-39 -0.844495118921138 2215 40-41 -0.801627307481148 2215 42-43 -0.988333076749278 2215 44-45 -0.9258776791830243 2215 46-47 -0.9251651236884371 2215 48-49 -0.5474481273690657 2215 50-51 -0.758220212395571 2215 52-53 -1.116847384275971 2215 54-55 -0.9770466013082171 2215 56-57 -0.9811225235979251 2215 58-59 -0.9058419488936167 2215 60-61 -0.7520601719226434 2215 62-63 -0.843180523572542 2215 64-65 -0.7480152386038412 2215 66-67 -0.8432998835823469 2215 68-69 -0.8530965456516739 2215 70-71 -0.7136397591522723 2215 72-73 -0.6560232601446998 2215 74-75 -0.6334516405669035 2215 76-77 -0.6376546316011265 2215 78-79 -0.6317085293827205 2215 80-81 -0.48656549507139957 2215 82-83 -0.5702884141124969 2215 84-85 -0.6854233245149857 2215 86-87 -0.6819145891196676 2215 88-89 -0.7369048000151359 2215 90-91 -0.6711760840238057 2215 92-93 -0.6505797627819234 2215 94-95 -0.851116569875483 2215 96-97 -0.8724559444877897 2215 98-99 -0.9524203525180752 2215 100-101 -0.7559841178520337 2216 1 -1.4198247674272153 2216 2 -4.546955882846522 2216 3 -2.082440846986948 2216 4 -1.3879224253908333 2216 5 -1.2810758303723588 2216 6 -1.0847955615844214 2216 7 -1.0756918036899066 2216 8 -1.159847369897463 2216 9 -3.701046552092663 2216 10-11 -1.7191117964799858 2216 12-13 -1.3400972283970987 2216 14-15 -1.632185584545553 2216 16-17 -1.498091926343747 2216 18-19 -1.3269478194829105 2216 20-21 -1.2653320063003548 2216 22-23 -4.201855645033987 2216 24-25 -1.92638797378995 2216 26-27 -1.4693673705651449 2216 28-29 -1.4549353479828895 2216 30-31 -1.6917272894492825 2216 32-33 -1.5758250064562276 2216 34-35 -1.7743932369504734 2216 36-37 -1.7607261917484252 2216 38-39 -1.6926159924107864 2216 40-41 -1.5577251925275633 2216 42-43 -1.769332101058012 2216 44-45 -1.546990209486438 2216 46-47 -1.3373825112633142 2216 48-49 -1.5053365554530558 2216 50-51 -1.8347438122835484 2216 52-53 -1.7979583770066085 2216 54-55 -1.620019671289782 2216 56-57 -1.4854501605784733 2216 58-59 -1.571790607806335 2216 60-61 -1.5303095489214016 2216 62-63 -1.2930579997877487 2216 64-65 -1.3941638165838697 2216 66-67 -1.3976249620615988 2216 68-69 -1.3365496547498807 2216 70-71 -1.12995754676799 2216 72-73 -1.3713043356150507 2216 74-75 -1.1948189629926986 2216 76-77 -1.2799610080538173 2216 78-79 -1.1384312696636911 2216 80-81 -1.127896983647016 2216 82-83 -1.1278890540591817 2216 84-85 -1.1111170306264953 2216 86-87 -1.2244459035688742 2216 88-89 -1.234000962831729 2216 90-91 -1.243723315564715 2216 92-93 -1.1991725623434917 2216 94-95 -1.390422071493763 2216 96-97 -1.376938327537708 2216 98-99 -1.4267562978299253 2216 100-101 -1.5548430880688535 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 41.0 11 486.0 12 1951.0 13 3747.0 14 5661.0 15 6754.0 16 7867.0 17 9403.0 18 12198.0 19 20465.0 20 20346.0 21 17996.0 22 19543.0 23 22616.0 24 25915.0 25 29742.0 26 34417.0 27 40994.0 28 48801.0 29 56207.0 30 65209.0 31 77506.0 32 95317.0 33 119582.0 34 155744.0 35 211329.0 36 318698.0 37 483447.0 38 452870.0 39 86008.0 40 92.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.567126569594183 40.53649357474157 13.338898517800429 20.557481337863816 2 23.04047570087052 28.24608560265562 31.889812611589292 16.82362608488457 3 22.472573922296316 29.335743825256472 22.92276633732525 25.26891591512196 4 24.431119010082615 26.475630693705952 26.37942317923811 22.713827116973324 5 22.924172259736018 28.444603113078603 24.48315959117893 24.148065036006447 6 22.369022322754585 25.28017684556858 27.261855801337603 25.088945030339232 7 25.14488247831863 25.763703246738412 24.221894186422254 24.869520088520705 8 26.611210664264334 25.018237811266808 25.739794169775664 22.630757354693195 9 21.7443262862757 26.933493597589838 26.05558982795257 25.266590288181902 10-11 25.59744132076026 25.436095851734347 25.186315358277113 23.780147469228282 12-13 24.650401150246925 25.595584899255474 24.97180687341082 24.78220707708678 14-15 24.48740230587612 25.512954716686277 25.173089638572797 24.826553338864805 16-17 24.58934257275144 26.685837480028134 25.12586966349347 23.59895028372695 18-19 23.03368081764214 25.34655949733777 26.677247597870213 24.942512087149883 20-21 23.037542766554132 27.904917743170355 25.44760677638868 23.60993271388683 22-23 21.841621272447338 26.921135458744477 27.967871951371293 23.26937131743689 24-25 21.784983957254163 25.78636790928586 29.14051356370912 23.288134569750856 26-27 21.716391253107723 25.63260661527028 30.674630107788797 21.9763720238332 28-29 21.719327123488004 25.417813942936235 31.000023666398448 21.862835267177317 30-31 21.806896481376643 25.382499196719554 30.884383718142917 21.926220603760882 32-33 21.79174977218022 25.377970709730775 30.93520248572952 21.895077032359485 34-35 21.845713887031316 25.472627295713007 30.848552404865586 21.833106412390087 36-37 21.80342339357028 25.43508619289481 30.745349599295473 22.01614081423944 38-39 21.93550927266114 25.505688324262326 30.503012821807456 22.055789581269075 40-41 22.413657685130577 25.939752299003814 29.13347900780061 22.513111008065 42-43 22.38430666620698 26.09741563633552 29.036641483929813 22.481636213527683 44-45 22.452296087397876 26.34772121200252 28.731570426511823 22.468412274087783 46-47 22.420002513309115 26.685141120674743 28.48209593659933 22.412760429416814 48-49 22.530658662403386 26.805896615407715 28.039383945935313 22.624060776253586 50-51 22.530161700867165 26.9049165117755 27.91327482048027 22.651646966877056 52-53 22.567843025893612 27.05538500957995 27.76433402204531 22.612437942481126 54-55 22.68359396675251 26.92386468604852 27.552457167663828 22.840084179535136 56-57 22.72108960110194 26.90219963508057 27.587565974364246 22.789144789453243 58-59 22.720436793539818 26.99602032189941 27.461635315583493 22.821907568977277 60-61 22.758768837578213 26.953302227053 27.333725833880056 22.954203101488726 62-63 22.7834735237573 26.94291850676798 27.397802976149677 22.875804993325044 64-65 22.805383377561046 27.190964980138332 27.235661897907427 22.767989744393198 66-67 22.862871243500486 27.053712190202013 27.11913574806851 22.964280818229 68-69 22.819090556722905 27.049960219506726 27.218486709235197 22.912462514535182 70-71 22.840895157158887 27.21369083092095 27.140610281751837 22.80480373016833 72-73 22.898780994606067 27.151518712490706 26.990694299169316 22.959005993733907 74-75 22.933068456665 27.094737065434167 27.103611168231772 22.86858330966906 76-77 22.940578743054477 27.307682186026273 26.932745860328822 22.818993210590428 78-79 23.00392115422003 27.216004984217257 26.86619991990817 22.913873941654547 80-81 22.89479761333555 27.21915810672751 26.987941828318142 22.898102451618797 82-83 22.973889329533996 27.308449941084117 26.904076456821674 22.813584272560213 84-85 23.02773333920861 27.22390388376094 26.835564509910242 22.912798267120206 86-87 23.034641233284045 27.207978777226156 26.896507969148313 22.860872020341482 88-89 23.080623365941072 27.25965257581544 26.83037040301075 22.829353655232744 90-91 23.111856127741383 27.219668112635414 26.76233561489576 22.906140144727434 92-93 23.096666136251436 27.18510675626604 26.871794557939705 22.846432549542822 94-95 23.19961809246161 27.305817765636892 26.743646604960308 22.75091753694119 96-97 23.24748699635882 27.2287351500621 26.690044931313682 22.8337329222654 98-99 23.26154579408972 27.2088470997641 26.773935153314827 22.755671952831356 100-101 23.337422767044718 27.357699338466755 26.560300577459056 22.74457731702947 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 165.0 1 139.5 2 90.5 3 62.0 4 51.5 5 44.0 6 37.5 7 39.0 8 41.0 9 39.0 10 52.5 11 64.0 12 61.0 13 72.0 14 99.0 15 138.5 16 338.5 17 494.0 18 604.5 19 927.0 20 1359.5 21 1934.0 22 2685.5 23 3760.5 24 5059.0 25 6745.0 26 9001.5 27 11602.5 28 14769.5 29 18615.0 30 23338.0 31 28333.0 32 33201.0 33 38508.5 34 44304.5 35 50640.5 36 57448.5 37 64278.5 38 71283.5 39 78063.5 40 84215.5 41 88563.0 42 92355.0 43 96322.5 44 99276.5 45 101956.0 46 103465.0 47 103761.0 48 102601.0 49 99968.0 50 96633.0 51 93465.0 52 90823.0 53 86994.5 54 81453.5 55 74699.5 56 66593.5 57 58079.0 58 50537.5 59 43448.5 60 36003.5 61 28903.5 62 23075.5 63 18408.0 64 14314.0 65 10978.0 66 8427.0 67 6299.0 68 4756.5 69 3738.5 70 2995.5 71 2430.5 72 1946.0 73 1562.0 74 1226.5 75 846.0 76 528.0 77 360.5 78 232.0 79 137.5 80 77.0 81 32.5 82 14.5 83 8.5 84 4.0 85 2.0 86 3.0 87 3.5 88 1.5 89 0.0 90 0.5 91 1.0 92 0.5 93 1.0 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 6.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 8.160094526534995E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.005161259788033385 16-17 1.632018905306999E-4 18-19 8.160094526534995E-5 20-21 2.040023631633749E-5 22-23 0.015626581018314514 24-25 0.0 26-27 6.120070894901246E-5 28-29 0.008976103979188495 30-31 0.002631630484807536 32-33 6.120070894901246E-5 34-35 0.0010200118158168745 36-37 0.021134644823725637 38-39 4.284049626430872E-4 40-41 0.0021828252858481115 42-43 2.040023631633749E-5 44-45 0.0 46-47 0.0 48-49 0.010526521939230144 50-51 0.0015300177237253115 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 8.160094526534995E-5 70-71 0.009445309414464257 72-73 0.0058140673501561844 74-75 0.0 76-77 1.0200118158168745E-4 78-79 6.324073258064621E-4 80-81 0.0 82-83 0.0 84-85 1.2240141789802492E-4 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0017136198505723489 94-95 0.003957645845369472 96-97 0.004080047263267498 98-99 0.013912961167742167 100-101 0.029988347385016108 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2450952.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.59465394172832 #Duplication Level Percentage of deduplicated Percentage of total 1 87.59872510691362 67.97192760407552 2 7.750677562426593 12.028222865408198 3 2.0835397043769417 4.850146270049392 4 0.8870966575735533 2.75335832629135 5 0.5019427554288051 1.9474037203027852 6 0.30697648954327866 1.4291840684614376 7 0.1958050323063742 1.0635396605303429 8 0.14693462103042307 0.912107285673174 9 0.10940395953225247 0.7640246141783983 >10 0.41431628380987584 4.819384870867427 >50 0.0022613565870887352 0.11092632892161756 >100 0.0017414524513079683 0.27144467189778826 >500 2.6319000903312934E-4 0.1598644936949152 >1k 2.6319000903144167E-4 0.24674026734966148 >5k 0.0 0.0 >10k+ 5.263800180628833E-5 0.6717249522980521 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 16446 0.6710045729169727 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 1.2240141789802492E-4 0.0 0.0 0.0 0.0 10-11 3.264037810613998E-4 0.0 0.0 0.0 0.0 12-13 6.324073258064621E-4 0.0 0.0 0.0 0.0 14-15 8.77210161602512E-4 0.0 0.0 0.0 0.0 16-17 0.0012648146516129242 0.0 0.0 0.0 0.0 18-19 0.0016524191416233366 0.0 0.0 4.080047263267498E-5 0.0 20-21 0.0019584226863683988 0.0 0.0 4.080047263267498E-5 0.0 22-23 0.002509229066909511 0.0 0.0 4.080047263267498E-5 0.0 24-25 0.0031008359200832983 0.0 0.0 4.080047263267498E-5 0.0 26-27 0.003672042536940748 0.0 0.0 4.080047263267498E-5 0.0 28-29 0.004447251516961573 0.0 0.0 4.080047263267498E-5 0.0 30-31 0.005161259788033385 0.0 0.0 4.080047263267498E-5 0.0 32-33 0.005854867822788859 0.0 0.0 4.080047263267498E-5 0.0 34-35 0.007119682474401784 0.0 0.0 4.080047263267498E-5 0.0 36-37 0.008710900907076108 0.0 0.0 4.080047263267498E-5 0.0 38-39 0.011199729737669282 0.0 0.0 4.080047263267498E-5 0.0 40-41 0.014239364948803568 0.0 0.0 4.080047263267498E-5 0.0 42-43 0.018054209139958678 0.0 0.0 4.080047263267498E-5 0.0 44-45 0.0233990710548391 0.0 0.0 4.080047263267498E-5 0.0 46-47 0.029927146676067096 0.0 0.0 4.080047263267498E-5 0.0 48-49 0.03839324474734715 0.0 0.0 1.0200118158168743E-4 0.0 50-51 0.04971537590291446 0.0 0.0 1.2240141789802492E-4 0.0 52-53 0.06126190965796148 0.0 0.0 1.2240141789802492E-4 0.0 54-55 0.07705169256680669 0.0 0.0 1.2240141789802492E-4 0.0 56-57 0.0957179087962555 0.0 0.0 1.2240141789802492E-4 0.0 58-59 0.11750536118210393 0.0 0.0 1.2240141789802492E-4 0.0 60-61 0.14341366130385252 0.0 0.0 1.2240141789802492E-4 0.0 62-63 0.17303480443517458 0.0 0.0 1.2240141789802492E-4 0.0 64-65 0.2065115922302844 0.0 0.0 1.2240141789802492E-4 0.0 66-67 0.24704686179084698 0.0 0.0 1.4280165421436242E-4 0.0 68-69 0.29480381500739306 0.0 0.0 1.632018905306999E-4 0.0 70-71 0.347028419977217 0.0 0.0 1.632018905306999E-4 2.040023631633749E-5 72-73 0.4072703178193616 0.0 0.0 1.632018905306999E-4 4.080047263267498E-5 74-75 0.47761033263809327 0.0 0.0 1.632018905306999E-4 4.080047263267498E-5 76-77 0.5528668044090623 0.0 0.0 1.632018905306999E-4 4.080047263267498E-5 78-79 0.6369361782686891 0.0 0.0 1.632018905306999E-4 4.080047263267498E-5 80-81 0.7292472476001162 2.040023631633749E-5 0.0 1.632018905306999E-4 4.080047263267498E-5 82-83 0.832594844778682 4.080047263267498E-5 0.0 1.632018905306999E-4 4.080047263267498E-5 84-85 0.9471421716949169 4.080047263267498E-5 0.0 1.632018905306999E-4 4.080047263267498E-5 86-87 1.0740112413462197 4.080047263267498E-5 0.0 1.632018905306999E-4 4.080047263267498E-5 88-89 1.2144668683842033 4.080047263267498E-5 0.0 1.632018905306999E-4 8.160094526534995E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 6915 0.0 88.87709 1 TATCTCG 6335 0.0 87.71764 2 ATCTCGA 6750 0.0 82.53573 3 ATATCTC 6930 0.0 80.52901 1 CTCGACG 1670 0.0 80.485504 5 TCGAGGG 5355 0.0 78.4931 6 TCTCGAG 5505 0.0 76.69943 4 CTCGAGG 5485 0.0 76.632744 5 AGGGCGC 5520 0.0 76.49725 9 CGAGGGC 5735 0.0 73.623436 7 TCTCGAC 1820 0.0 73.59112 4 TCGACGG 8465 0.0 72.71529 2 GAGGGCG 5945 0.0 70.785995 8 CGACGGC 8840 0.0 69.79183 3 ACGGCGC 9130 0.0 67.575005 5 GACGGCG 9145 0.0 67.41223 4 CGGCGCG 9270 0.0 66.70816 6 CGCGCCG 14420 0.0 43.216717 9 GGCGCGC 14515 0.0 42.93036 7 GCGCGCC 14525 0.0 42.86985 8 >>END_MODULE