FastQCFastQC Report
Tue 31 May 2016
SRR2048262_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048262_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2450952
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT112050.45716929584912314No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT75790.30922678208304366No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT31110.12693027036025187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG17950.076.54721
TCGACGG21100.066.407772
TATCTCG26700.062.975462
ATATCTC27600.061.4971661
CGACGGC24750.056.4223863
ACGGCGC27700.050.5849765
GACGGCG28000.049.8733634
CGGCGCG28550.049.2453166
ATCTCGA41200.040.927053
TCTCGAG41150.039.4762234
CTCGAGG44500.036.504425
TCGAGGG44500.036.3976826
CGAGGGC47550.034.0630237
AGGGCGC48450.033.528319
GAGGGCG51300.031.5730368
AGTCGAC4150.026.34971
GGGCGCG45150.025.0379378
CGCGCCG73350.023.1178519
GGCGCGC74250.022.8376337
GCGCGCC75200.022.612298
Produced by FastQC (version 0.11.5)