##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048262_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2450952 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18570702323016 33.0 31.0 34.0 31.0 34.0 2 32.39775972764868 34.0 31.0 34.0 31.0 34.0 3 32.47457763350731 34.0 31.0 34.0 31.0 34.0 4 36.08054176499581 37.0 35.0 37.0 35.0 37.0 5 35.89215088667587 37.0 35.0 37.0 35.0 37.0 6 35.82502146104861 37.0 35.0 37.0 35.0 37.0 7 35.7573954936694 37.0 35.0 37.0 33.0 37.0 8 35.7097291175021 37.0 35.0 37.0 33.0 37.0 9 37.26965562769079 39.0 37.0 39.0 33.0 39.0 10-11 37.27136353547519 39.0 37.0 39.0 33.5 39.0 12-13 37.24484894033012 39.0 37.0 39.0 33.0 39.0 14-15 38.5428311121556 40.0 38.0 41.0 34.0 41.0 16-17 38.54693665971426 40.0 38.0 41.0 34.0 41.0 18-19 38.56513367050844 40.0 38.0 41.0 34.0 41.0 20-21 38.60637642026445 40.0 38.0 41.0 34.0 41.0 22-23 38.57460346020648 40.0 38.0 41.0 34.0 41.0 24-25 38.56448473899122 40.0 38.0 41.0 34.0 41.0 26-27 38.421341788823284 40.0 38.0 41.0 34.0 41.0 28-29 38.355095285423786 40.0 38.0 41.0 34.0 41.0 30-31 38.189889479679735 40.0 37.5 41.0 34.0 41.0 32-33 38.04837875241947 40.0 37.0 41.0 33.5 41.0 34-35 37.97160776710437 40.0 37.0 41.0 33.0 41.0 36-37 37.95116775032722 40.0 37.0 41.0 33.0 41.0 38-39 37.90791945333895 40.0 37.0 41.0 33.0 41.0 40-41 37.778195982622265 40.0 37.0 41.0 33.0 41.0 42-43 37.79724551929209 40.0 37.0 41.0 33.0 41.0 44-45 37.69076444581534 40.0 37.0 41.0 33.0 41.0 46-47 37.705539112965084 40.0 37.0 41.0 33.0 41.0 48-49 37.66273941717341 40.0 37.0 41.0 33.0 41.0 50-51 37.5769802917397 40.0 36.0 41.0 32.0 41.0 52-53 37.40684211685908 39.0 36.0 41.0 32.0 41.0 54-55 37.25026132702721 39.0 36.0 41.0 32.0 41.0 56-57 36.94281834160767 39.0 35.0 41.0 31.0 41.0 58-59 36.77096246682922 39.0 35.0 40.0 31.0 41.0 60-61 36.51693525617801 38.5 35.0 40.0 31.0 41.0 62-63 36.19219429837875 38.0 35.0 40.0 30.5 41.0 64-65 35.96552217260884 37.5 35.0 40.0 30.0 41.0 66-67 35.79252510861086 37.0 35.0 40.0 30.5 41.0 68-69 35.462250994715525 37.0 34.0 39.0 30.0 41.0 70-71 35.16635454305103 36.0 34.0 39.0 30.0 41.0 72-73 34.84924000959627 36.0 34.0 39.0 30.0 40.0 74-75 34.50477977536892 35.0 34.0 37.5 30.0 39.5 76-77 34.12078061912269 35.0 34.0 37.0 29.5 39.0 78-79 33.81891342629313 35.0 34.0 36.5 29.0 39.0 80-81 33.55836507610104 35.0 34.0 36.0 29.0 38.0 82-83 33.31093836190998 35.0 33.0 36.0 29.0 37.0 84-85 33.06116704855909 35.0 33.0 35.0 29.0 37.0 86-87 32.83516527455454 35.0 33.0 35.0 29.0 36.0 88-89 32.69046333832731 35.0 33.0 35.0 29.0 36.0 90-91 32.546896063244 35.0 33.0 35.0 29.0 36.0 92-93 32.44899512515953 35.0 33.0 35.0 29.0 35.5 94-95 32.360760839053555 35.0 33.0 35.0 29.0 35.0 96-97 32.22881109054767 35.0 33.0 35.0 29.0 35.0 98-99 32.072127279522405 35.0 33.0 35.0 29.0 35.0 100-101 31.45803324585712 34.5 32.0 35.0 27.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4511700919349195 1101 2 0.46144409478211657 1101 3 0.48532670791285426 1101 4 0.33729059903966174 1101 5 0.40661076479965885 1101 6 0.4393169883644745 1101 7 0.49086453016568043 1101 8 0.47380336615637475 1101 9 0.7061076720185255 1101 10-11 0.6115353098311758 1101 12-13 0.6273044875808509 1101 14-15 0.787328288420035 1101 16-17 0.8262090691710569 1101 18-19 0.795824945346574 1101 20-21 0.7654396097188041 1101 22-23 0.7710241886050895 1101 24-25 0.8048316244127847 1101 26-27 0.9732600509993503 1101 28-29 0.9801477455175345 1101 30-31 0.9844757575462566 1101 32-33 1.0356911668465543 1101 34-35 1.0785042617161338 1101 36-37 0.9440909061924003 1101 38-39 0.8996089984412592 1101 40-41 0.8927008767149829 1101 42-43 0.8247437682080374 1101 44-45 0.9811305396675252 1101 46-47 0.8294880972050294 1101 48-49 0.778910365159085 1101 50-51 0.8323507722335464 1101 52-53 0.7297419493588535 1101 54-55 0.78266673205475 1101 56-57 0.8780407999484652 1101 58-59 0.7539640876918554 1101 60-61 0.7801466202121645 1101 62-63 0.758744856094772 1101 64-65 0.7683193481719357 1101 66-67 0.7143340931348305 1101 68-69 0.8225673646945992 1101 70-71 0.7100130872754278 1101 72-73 0.6525055319801041 1101 74-75 0.6556492415464419 1101 76-77 0.6455228320047723 1101 78-79 0.6294578855962598 1101 80-81 0.5708415784320593 1101 82-83 0.5750160983075574 1101 84-85 0.6167661611117694 1101 86-87 0.6967497821717004 1101 88-89 0.6685421859249701 1101 90-91 0.6568513936854359 1101 92-93 0.6504684219246926 1101 94-95 0.7113890351290237 1101 96-97 0.7043160835420323 1101 98-99 0.6861358874543413 1101 100-101 0.801422583372986 1102 1 0.4801776357771672 1102 2 0.497276400009639 1102 3 0.4588012443309353 1102 4 0.3361545291430019 1102 5 0.3872527521812046 1102 6 0.4318613489241869 1102 7 0.4885911668782441 1102 8 0.4212525144042658 1102 9 0.6341769515940427 1102 10-11 0.6419465351004661 1102 12-13 0.6184808426895714 1102 14-15 0.7276189950701877 1102 16-17 0.7641795304639345 1102 18-19 0.8206555080960243 1102 20-21 0.7429986143907712 1102 22-23 0.8046754487372922 1102 24-25 0.752634056585606 1102 26-27 0.8519597303549133 1102 28-29 0.8393648661153392 1102 30-31 0.9585847868883164 1102 32-33 1.000274571550385 1102 34-35 0.9772103499563087 1102 36-37 0.8560052251221677 1102 38-39 0.8693295490492048 1102 40-41 0.9760333387722042 1102 42-43 0.9065513395010356 1102 44-45 1.0488436534333445 1102 46-47 0.931685114459853 1102 48-49 0.8443625174187019 1102 50-51 0.9375020550559938 1102 52-53 0.9110817677300389 1102 54-55 0.8234469865059424 1102 56-57 0.9441176849858479 1102 58-59 0.8833359102220371 1102 60-61 0.7911048452291922 1102 62-63 0.797539824508597 1102 64-65 0.8678634130909799 1102 66-67 0.6802180442733743 1102 68-69 0.7863466835064656 1102 70-71 0.7301989605009709 1102 72-73 0.6610219408157789 1102 74-75 0.6378245729242593 1102 76-77 0.685824422191196 1102 78-79 0.6750880985153813 1102 80-81 0.641097613483602 1102 82-83 0.658600544538551 1102 84-85 0.6208677304765473 1102 86-87 0.6653226674955945 1102 88-89 0.7242522663152045 1102 90-91 0.651642673375747 1102 92-93 0.6522411258600229 1102 94-95 0.6398662109575852 1102 96-97 0.7653724439095413 1102 98-99 0.759480911459292 1102 100-101 0.8384662868051258 1103 1 0.34991415286746985 1103 2 0.3615406781590309 1103 3 0.373845605934477 1103 4 0.2603200184561345 1103 5 0.3069891514332781 1103 6 0.2922600843067755 1103 7 0.2908197346578376 1103 8 0.3242929202346332 1103 9 0.42226897225142324 1103 10-11 0.49175893664241954 1103 12-13 0.5045061379556941 1103 14-15 0.6536236866028204 1103 16-17 0.6687920385316417 1103 18-19 0.6493935246497173 1103 20-21 0.6242212163014784 1103 22-23 0.6802612309828007 1103 24-25 0.651548244656091 1103 26-27 0.7700655852201947 1103 28-29 0.7284314538042125 1103 30-31 0.7459955061941699 1103 32-33 0.8324263334956115 1103 34-35 0.8386444063936409 1103 36-37 0.7417372995990732 1103 38-39 0.7294331838422679 1103 40-41 0.7779738762559276 1103 42-43 0.7535650270284009 1103 44-45 0.8504061266041063 1103 46-47 0.8210759536711691 1103 48-49 0.741723878219041 1103 50-51 0.7244854424471541 1103 52-53 0.7658959531183029 1103 54-55 0.7205405459061183 1103 56-57 0.9268347730214401 1103 58-59 0.7627713551303685 1103 60-61 0.6970541718735035 1103 62-63 0.7689236807387729 1103 64-65 0.8472533948889023 1103 66-67 0.6524025832021252 1103 68-69 0.7041528925934486 1103 70-71 0.640802251823672 1103 72-73 0.6313282225696781 1103 74-75 0.6171749947458309 1103 76-77 0.6107717400865269 1103 78-79 0.5543255190882022 1103 80-81 0.5864705046983758 1103 82-83 0.5704046915687826 1103 84-85 0.5424126659522557 1103 86-87 0.5923627081396887 1103 88-89 0.5800314345591318 1103 90-91 0.5615790845014246 1103 92-93 0.5790743604149995 1103 94-95 0.5987542490420026 1103 96-97 0.6311320330514079 1103 98-99 0.6659304052655557 1103 100-101 0.7719727001787184 1104 1 0.3288346656741936 1104 2 0.4014132338747274 1104 3 0.386538047386388 1104 4 0.23655603127003388 1104 5 0.3139321019990433 1104 6 0.3391718337223679 1104 7 0.3317762860926763 1104 8 0.3368338823881132 1104 9 0.4526307119866857 1104 10-11 0.44012757630454047 1104 12-13 0.48091771886176815 1104 14-15 0.6233752762391234 1104 16-17 0.5354533944595943 1104 18-19 0.6361997001649229 1104 20-21 0.6244124140993819 1104 22-23 0.6237668473690903 1104 24-25 0.6746979115809069 1104 26-27 0.717079874468574 1104 28-29 0.7680756141428091 1104 30-31 0.7510059752959819 1104 32-33 0.8352707158544561 1104 34-35 0.8195646812254864 1104 36-37 0.7931560623269149 1104 38-39 0.7496979656224525 1104 40-41 0.797490224816066 1104 42-43 0.6307953132077557 1104 44-45 0.8612600328877136 1104 46-47 0.7989110142270306 1104 48-49 0.7547750746366049 1104 50-51 0.7982065627844577 1104 52-53 0.8248841991314393 1104 54-55 0.7491314791978567 1104 56-57 0.8639866059209496 1104 58-59 0.8215713724294815 1104 60-61 0.8128797026784795 1104 62-63 0.7770215489141421 1104 64-65 0.7875765454969681 1104 66-67 0.7741433133861193 1104 68-69 0.8443365634836155 1104 70-71 0.7820052148125285 1104 72-73 0.6892889974983234 1104 74-75 0.6530719471436939 1104 76-77 0.6726402775673463 1104 78-79 0.6604127348628523 1104 80-81 0.6464117145967023 1104 82-83 0.6031104950185693 1104 84-85 0.5964080893055268 1104 86-87 0.6336044036680946 1104 88-89 0.6897315212339663 1104 90-91 0.6613817020457802 1104 92-93 0.6481791715409244 1104 94-95 0.6548027098689175 1104 96-97 0.6202598641009018 1104 98-99 0.6598541470910533 1104 100-101 0.6914357396780595 1105 1 0.2665445068346628 1105 2 0.284028382586726 1105 3 0.2413171134106804 1105 4 0.18046491827206523 1105 5 0.20793208029718357 1105 6 0.25212443053074907 1105 7 0.2843763723575705 1105 8 0.31343136533406835 1105 9 0.35497076023715124 1105 10-11 0.3660890817263791 1105 12-13 0.39295363815255513 1105 14-15 0.48538979320093745 1105 16-17 0.4235389942809107 1105 18-19 0.5099764273616998 1105 20-21 0.48998654471383674 1105 22-23 0.46739488743795476 1105 24-25 0.3948658794549118 1105 26-27 0.5922151720010689 1105 28-29 0.6092391084948972 1105 30-31 0.5605784848267135 1105 32-33 0.6261769696974966 1105 34-35 0.6222524170071537 1105 36-37 0.5058976707150435 1105 38-39 0.43707727728466494 1105 40-41 0.5430336857292133 1105 42-43 0.49981317293416794 1105 44-45 0.6144709756436342 1105 46-47 0.5260635335416879 1105 48-49 0.44657532077186346 1105 50-51 0.4755867142634358 1105 52-53 0.4297960477591545 1105 54-55 0.49905806587508295 1105 56-57 0.6386497241074665 1105 58-59 0.6135993694111121 1105 60-61 0.46992843284839836 1105 62-63 0.5457319430199163 1105 64-65 0.5298416692066112 1105 66-67 0.49187682991932036 1105 68-69 0.5180733456945958 1105 70-71 0.4659787473598911 1105 72-73 0.39303430899235536 1105 74-75 0.40671460997094044 1105 76-77 0.4672183843694242 1105 78-79 0.4410119333860365 1105 80-81 0.4031441514623779 1105 82-83 0.42932383809061037 1105 84-85 0.46552518982853996 1105 86-87 0.4583309715370376 1105 88-89 0.5665207095934477 1105 90-91 0.3765052590981597 1105 92-93 0.4069870748157385 1105 94-95 0.41168118216649674 1105 96-97 0.43732807734156864 1105 98-99 0.34632593670779244 1105 100-101 0.369912485004928 1106 1 0.1328983681753968 1106 2 0.19880305470204718 1106 3 0.18958338168172162 1106 4 0.13624864190014563 1106 5 0.23053864360558407 1106 6 0.16462126854680292 1106 7 0.18139599508738513 1106 8 0.21488522918945563 1106 9 0.27470562781159913 1106 10-11 0.2546041876561489 1106 12-13 0.22500291607196488 1106 14-15 0.3281894146470137 1106 16-17 0.3288298675235666 1106 18-19 0.35301442479214984 1106 20-21 0.31288380960203455 1106 22-23 0.339593266320513 1106 24-25 0.37080331081129714 1106 26-27 0.4086909254546214 1106 28-29 0.4107392294626706 1106 30-31 0.45513055442056327 1106 32-33 0.5174909840122268 1106 34-35 0.489521316025872 1106 36-37 0.47635899943610127 1106 38-39 0.4262458101390436 1106 40-41 0.5931157509103429 1106 42-43 0.5347831078205445 1106 44-45 0.6252485039074358 1106 46-47 0.5122159384694527 1106 48-49 0.5060001227366513 1106 50-51 0.45633547091336624 1106 52-53 0.4211763618298505 1106 54-55 0.4317275289814049 1106 56-57 0.5547803318709299 1106 58-59 0.41323390549825945 1106 60-61 0.3972464354827068 1106 62-63 0.4871736653162557 1106 64-65 0.4962311721203463 1106 66-67 0.449400348018159 1106 68-69 0.4297705161050587 1106 70-71 0.33861483782840196 1106 72-73 0.37702198210631366 1106 74-75 0.2830905107560042 1106 76-77 0.3238097099867687 1106 78-79 0.3632051681240327 1106 80-81 0.27514698395696513 1106 82-83 0.28494934482617396 1106 84-85 0.39128393105636405 1106 86-87 0.3848031470543347 1106 88-89 0.41112514497134356 1106 90-91 0.3989414513242764 1106 92-93 0.33377771651446864 1106 94-95 0.263366021557232 1106 96-97 0.2657117790840644 1106 98-99 0.25019017102081875 1106 100-101 0.2897947013182751 1107 1 0.12584985659811565 1107 2 0.1285487838357824 1107 3 0.09739701960551628 1107 4 0.050228152574881335 1107 5 0.07493410489210817 1107 6 0.1017934213860272 1107 7 0.13954271259763118 1107 8 0.08465967705448207 1107 9 0.16694050205381927 1107 10-11 0.09771828603340538 1107 12-13 0.1332839840225617 1107 14-15 0.15944140532373297 1107 16-17 0.17064479361021512 1107 18-19 0.25000175452122164 1107 20-21 0.2544995944246651 1107 22-23 0.2047225565416042 1107 24-25 0.21814202993704157 1107 26-27 0.29204336339702053 1107 28-29 0.28656212590980346 1107 30-31 0.35868280479044046 1107 32-33 0.4309047451004062 1107 34-35 0.37541563839510417 1107 36-37 0.34690160565802586 1107 38-39 0.39000598577192847 1107 40-41 0.35718133535536367 1107 42-43 0.3262483518561652 1107 44-45 0.39452031510091246 1107 46-47 0.2752273252874531 1107 48-49 0.23347219733235391 1107 50-51 0.423153708661836 1107 52-53 0.36766529381449686 1107 54-55 0.3213119551631394 1107 56-57 0.38272672475000036 1107 58-59 0.24280189406258046 1107 60-61 0.2824682402883596 1107 62-63 0.28121997441764535 1107 64-65 0.3080763241123776 1107 66-67 0.20250430240729855 1107 68-69 0.22448966657697156 1107 70-71 0.25772091874598857 1107 72-73 0.197241340697758 1107 74-75 0.17704653659697556 1107 76-77 0.2746531387371789 1107 78-79 0.19414783486841003 1107 80-81 0.11095343689928683 1107 82-83 0.2027857493210803 1107 84-85 0.25426214238218847 1107 86-87 0.2772334534993277 1107 88-89 0.29344837729457396 1107 90-91 0.2886711490711136 1107 92-93 0.2196765427437768 1107 94-95 0.2618590829633902 1107 96-97 0.20790193853249406 1107 98-99 0.25039525855761013 1107 100-101 0.24626583569638072 1108 1 0.07682428981305378 1108 2 0.10241177331819529 1108 3 0.08251574786817173 1108 4 0.06798874154514323 1108 5 0.150659329699522 1108 6 0.09032634878382595 1108 7 0.12143996410609503 1108 8 0.09805821711727702 1108 9 0.18188039679914425 1108 10-11 0.13860356013992003 1108 12-13 0.20301447580715148 1108 14-15 0.23155513153427876 1108 16-17 0.218591775859025 1108 18-19 0.17114013816303952 1108 20-21 0.1653099317161164 1108 22-23 0.1868402354099814 1108 24-25 0.08931796989059393 1108 26-27 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-0.7569636206656085 2215 34-35 -0.8095173708746941 2215 36-37 -0.9211353437331553 2215 38-39 -0.902799538962114 2215 40-41 -0.837397673063144 2215 42-43 -0.839865306329088 2215 44-45 -0.7361063789856459 2215 46-47 -0.6272102381907629 2215 48-49 -1.0083987701066093 2215 50-51 -0.8077959105008929 2215 52-53 -0.7578097975365026 2215 54-55 -0.7078077410038119 2215 56-57 -0.5474630983285564 2215 58-59 -0.56229664205879 2215 60-61 -0.5576144292142189 2215 62-63 -0.5410463158161676 2215 64-65 -0.4430223426476445 2215 66-67 -0.5232277970027326 2215 68-69 -0.3820718516319701 2215 70-71 -0.33761630613905425 2215 72-73 -0.47575936500709304 2215 74-75 -0.5338644374336852 2215 76-77 -0.4797161476897642 2215 78-79 -0.45766422137109686 2215 80-81 -0.5568011451659061 2215 82-83 -0.5468925850276207 2215 84-85 -0.46871531732794836 2215 86-87 -0.4065928414989628 2215 88-89 -0.4401756199150526 2215 90-91 -0.5297982099755529 2215 92-93 -0.6672870863466329 2215 94-95 -0.6310188053354437 2215 96-97 -0.5478481898648084 2215 98-99 -0.5683300839312437 2215 100-101 -0.8250200883308416 2216 1 -0.5713853655201042 2216 2 -0.5020255547020405 2216 3 -0.5668926540289014 2216 4 -0.4289483995548693 2216 5 -0.447946965071381 2216 6 -0.5358862193455209 2216 7 -0.6293318496588824 2216 8 -1.1427294243146378 2216 9 -0.8207898418865653 2216 10-11 -0.7918299999899432 2216 12-13 -0.8542863997517216 2216 14-15 -0.9856528315127377 2216 16-17 -0.997986011560215 2216 18-19 -0.9705432811184949 2216 20-21 -1.0540392770233993 2216 22-23 -0.9486749921816298 2216 24-25 -0.93119323321217 2216 26-27 -0.910399629107232 2216 28-29 -0.942097548427256 2216 30-31 -0.9383476960739898 2216 32-33 -0.8269290524701773 2216 34-35 -0.9551603049491604 2216 36-37 -1.0083040394438 2216 38-39 -1.008304716899083 2216 40-41 -1.025068811462745 2216 42-43 -0.8947866268519533 2216 44-45 -0.7052197062560239 2216 46-47 -0.8686980919839087 2216 48-49 -1.0580514319244045 2216 50-51 -1.115909795697597 2216 52-53 -1.0357417102547757 2216 54-55 -1.1544744216783727 2216 56-57 -0.941309025132675 2216 58-59 -1.053269149417261 2216 60-61 -0.9725335076494659 2216 62-63 -0.9655953088914089 2216 64-65 -0.8406127658929776 2216 66-67 -0.8711776093422259 2216 68-69 -0.5659216149520532 2216 70-71 -0.6812021433634925 2216 72-73 -0.6306944791891738 2216 74-75 -0.6948851385520314 2216 76-77 -0.628754472556146 2216 78-79 -0.6216474074443497 2216 80-81 -0.6062818806398624 2216 82-83 -0.6631820313704111 2216 84-85 -0.5476578133424965 2216 86-87 -0.5499635915749153 2216 88-89 -0.6568705454552486 2216 90-91 -0.6127610756156727 2216 92-93 -0.7071045972239745 2216 94-95 -0.7001166332790731 2216 96-97 -0.7464998720361606 2216 98-99 -0.8149740594516999 2216 100-101 -0.9734422716989961 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 14.0 13 91.0 14 297.0 15 747.0 16 1383.0 17 2283.0 18 3992.0 19 7559.0 20 8704.0 21 8496.0 22 8264.0 23 8750.0 24 9479.0 25 11449.0 26 14460.0 27 19492.0 28 26017.0 29 34271.0 30 45775.0 31 60641.0 32 81067.0 33 108431.0 34 149576.0 35 210875.0 36 325584.0 37 553808.0 38 620065.0 39 129270.0 40 111.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 23.71249498735302 40.6541800371283 14.388277389062262 21.24504758645642 2 22.822193172285708 29.223868929297677 29.82090224533161 18.133035653085006 3 21.931192450933352 28.51667433715552 24.474204309182717 25.07792890272841 4 22.283912536842827 27.58776997672741 26.27268914283103 23.85562834359873 5 23.45202141575401 27.54320239127898 25.57344100508139 23.431335187885622 6 23.452243639305724 26.367683401259345 26.370417034041072 23.80965592539386 7 24.046615355992284 27.206203956666634 25.016768994252033 23.730411693089053 8 24.081703762456385 26.141270820481182 26.42238607692031 23.354639340142118 9 23.04765658405387 26.123889819139666 26.788039912654348 24.040413684152117 10-11 24.190865426985106 26.29441539450793 25.9586071045047 23.556112074002264 12-13 23.83051565269332 26.510698699933737 25.715171084541844 23.943614562831094 14-15 23.604623835962517 26.39780379215913 25.977171319552568 24.02040105232579 16-17 23.717967548936087 26.60125534894196 25.89856920902572 23.78220789309623 18-19 23.316348096576352 26.491094072833736 26.491094072833736 23.70146375775617 20-21 22.865319271858446 26.93300399191824 26.627979658516367 23.57369707770695 22-23 22.563660977448762 26.79236476275341 27.232561061987344 23.411413197810482 24-25 22.51472081052587 26.326076561270884 28.07590683130473 23.08329579689851 26-27 22.45312824835923 26.186914122231236 28.695792503253585 22.664165126155943 28-29 22.448705645806204 26.306737137242997 28.7275515799575 22.517005636993296 30-31 22.499211519606742 26.266740162967572 28.569837330272712 22.664210987152973 32-33 22.412495226344703 26.296965424047475 28.65588554977821 22.634653799829618 34-35 22.535421023103993 26.380109144693222 28.61253633906258 22.471933493140206 36-37 22.556034197854352 26.373344801163974 28.459913322132067 22.610707678849604 38-39 22.55693350113211 26.38713985160457 28.473696847938445 22.582229799324875 40-41 22.64984430488824 26.602015386724464 28.092196312930856 22.655943995456447 42-43 22.643344300500377 26.575734653310224 27.99793712810369 22.782983918085705 44-45 22.579287558467076 26.613658692622295 28.00195597465801 22.805097774252616 46-47 22.64293629577405 26.865662811838014 27.839264090035222 22.652136802352718 48-49 22.760625259083 26.849424223730207 27.65372394073813 22.73622657644866 50-51 22.72102840039299 26.775146963302422 27.749054245044373 22.754770391260212 52-53 22.67319257291919 26.941599571921003 27.710995269384377 22.67421258577543 54-55 22.759369730361804 26.882055169605596 27.530966551350883 22.827608548681717 56-57 22.73434975470756 26.89518195378775 27.534688561832297 22.83577972967239 58-59 22.77527262875813 27.064157111195975 27.40174022175873 22.758830038287165 60-61 22.85955533178754 27.00396703157264 27.22857372505611 22.90790391158371 62-63 22.818166350241572 26.936833717082916 27.323561897930364 22.921438034745147 64-65 22.86121685609635 27.13838530382843 27.158195699369188 22.84220214070604 66-67 22.97121878144908 27.068593343673808 27.04564246447153 22.91454541040558 68-69 22.88145827952751 27.092653725525235 27.064052489184775 22.961835505762476 70-71 22.939244683481398 27.23303573395742 26.955000232563265 22.87271934999792 72-73 22.92218408283152 27.15433723481212 26.878423926059718 23.045054756296636 74-75 22.90650393235597 27.113590333209842 27.003224874619942 22.976680859814245 76-77 22.913221998250066 27.32726643286612 26.79382010930866 22.965691459575158 78-79 22.969735112104832 27.216774771337963 26.78108523557774 23.03240488097946 80-81 22.948286691809344 27.140872777464857 26.882360297281195 23.028480233444604 82-83 22.975113343712973 27.30049384892075 26.776024173464023 22.948368633902255 84-85 22.96499951141424 27.25453767649013 26.688349402262755 23.092113409832876 86-87 22.91487612055971 27.223998516494515 26.804732774189944 23.056392588755838 88-89 22.960139570256782 27.369875052632608 26.66029363284144 23.009691744269166 90-91 23.005172594592544 27.287568431708653 26.61823624194702 23.089022731751783 92-93 22.97643280630395 27.210089978934327 26.772435673813817 23.0410415409479 94-95 22.997893453548876 27.395467273456497 26.609806640823425 22.996832632171206 96-97 23.002678755577172 27.34317672136719 26.57906531402192 23.075079209033717 98-99 22.992433144345544 27.28486726790243 26.670595752181192 23.05210383557083 100-101 23.013840593975583 27.404947280484954 26.483342632477097 23.097869493062362 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 191.0 1 154.5 2 89.0 3 59.0 4 50.0 5 37.0 6 28.5 7 31.0 8 32.5 9 28.5 10 34.5 11 41.5 12 56.5 13 84.5 14 109.5 15 151.0 16 580.5 17 903.0 18 941.5 19 1223.5 20 1664.0 21 2300.5 22 3131.0 23 4162.0 24 5379.5 25 6948.5 26 8933.0 27 11447.5 28 14445.0 29 17813.5 30 21954.0 31 26438.0 32 31199.0 33 36885.0 34 42479.5 35 48522.0 36 55497.0 37 62466.5 38 69695.0 39 76739.5 40 83108.5 41 87835.5 42 91882.0 43 96389.5 44 99847.0 45 102363.0 46 104381.5 47 104746.0 48 103244.0 49 100942.5 50 97813.0 51 94838.0 52 92681.5 53 89244.5 54 83278.5 55 75630.5 56 67176.0 57 58508.5 58 51380.5 59 44505.5 60 36260.5 61 28764.0 62 23133.0 63 18596.5 64 14619.5 65 11253.5 66 8413.5 67 6195.5 68 4717.5 69 3770.0 70 3058.0 71 2499.0 72 2037.5 73 1606.0 74 1218.5 75 848.5 76 548.0 77 362.5 78 217.5 79 110.0 80 45.0 81 21.0 82 13.0 83 4.5 84 4.0 85 2.5 86 2.0 87 1.5 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0875578142697205 2 0.0 3 0.0 4 0.0 5 0.001876821741103049 6 4.080047263267498E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.007976492399687958 28-29 0.0 30-31 0.0014280165421436243 32-33 0.0 34-35 0.0031416363927159736 36-37 0.0015504179600416492 38-39 2.040023631633749E-5 40-41 3.264037810613998E-4 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.0200118158168745E-4 54-55 4.080047263267498E-5 56-57 0.0 58-59 0.0 60-61 4.080047263267498E-5 62-63 0.005508063805411121 64-65 0.008914903270239482 66-67 0.0026724309574402113 68-69 3.672042536940748E-4 70-71 2.4480283579604985E-4 72-73 4.080047263267498E-5 74-75 1.632018905306999E-4 76-77 1.0200118158168745E-4 78-79 3.876044900104123E-4 80-81 2.6520307211238734E-4 82-83 0.0 84-85 2.040023631633749E-5 86-87 2.040023631633749E-5 88-89 0.0 90-91 0.006160871367533922 92-93 0.001836021268470374 94-95 8.568099252861744E-4 96-97 2.040023631633749E-5 98-99 0.0 100-101 3.876044900104123E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2450952.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.89817095282947 #Duplication Level Percentage of deduplicated Percentage of total 1 86.46371338913727 65.62437700025191 2 8.33653905264865 12.654561323457326 3 2.2848088954582884 5.2023844842611595 4 1.0260287913182886 3.1149483442400188 5 0.540334032719881 2.050518239350264 6 0.331027712505496 1.5074638748319733 7 0.23990100261731506 1.2745633115882917 8 0.17402133627769364 1.0566320904195372 9 0.12782941164651004 0.8731816684151785 >10 0.47304694567946015 5.360394250182783 >50 0.001617772645571614 0.07927985328932995 >100 7.549844081575766E-4 0.12136614025938681 >500 1.0762083925739156E-4 0.06301785476426428 >1k 1.6143125888490317E-4 0.2501518862145274 >5k 5.381041962830106E-5 0.3095348809175352 >10k+ 5.381041962830106E-5 0.45762479755653546 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 11205 0.45716929584912314 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7579 0.30922678208304366 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 3111 0.12693027036025187 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.080047263267498E-5 0.0 0.0 0.0 0.0 2 4.080047263267498E-5 0.0 0.0 0.0 0.0 3 4.080047263267498E-5 0.0 0.0 0.0 0.0 4 4.080047263267498E-5 0.0 0.0 0.0 0.0 5 4.080047263267498E-5 0.0 0.0 0.0 0.0 6 4.080047263267498E-5 0.0 0.0 0.0 0.0 7 4.080047263267498E-5 0.0 0.0 0.0 0.0 8 4.080047263267498E-5 0.0 0.0 0.0 0.0 9 8.160094526534995E-5 0.0 0.0 0.0 0.0 10-11 2.4480283579604985E-4 0.0 0.0 0.0 0.0 12-13 3.672042536940748E-4 0.0 0.0 0.0 0.0 14-15 4.6920543527576226E-4 0.0 0.0 4.080047263267498E-5 0.0 16-17 8.36409688969837E-4 0.0 0.0 4.080047263267498E-5 0.0 18-19 0.0011424132337148995 0.0 0.0 4.080047263267498E-5 0.0 20-21 0.0014484167784599615 0.0 0.0 8.160094526534995E-5 0.0 22-23 0.001999223159001074 0.0 0.0 8.160094526534995E-5 0.0 24-25 0.0025500295395421858 0.0 0.0 8.160094526534995E-5 0.0 26-27 0.0031008359200832983 0.0 0.0 8.160094526534995E-5 0.0 28-29 0.0037740437185224355 0.0 0.0 8.160094526534995E-5 0.0 30-31 0.004365650571696222 2.040023631633749E-5 0.0 8.160094526534995E-5 0.0 32-33 0.004936857188553672 4.080047263267498E-5 0.0 8.160094526534995E-5 0.0 34-35 0.006201671840166597 4.080047263267498E-5 0.0 8.160094526534995E-5 0.0 36-37 0.007752089800208245 4.080047263267498E-5 0.0 8.160094526534995E-5 0.0 38-39 0.00999611579500537 4.080047263267498E-5 0.0 8.160094526534995E-5 0.0 40-41 0.012974550297190642 4.080047263267498E-5 0.0 1.0200118158168743E-4 0.0 42-43 0.016626192597815052 4.080047263267498E-5 0.0 1.2240141789802492E-4 0.0 44-45 0.021746651913215762 6.120070894901246E-5 0.0 1.2240141789802492E-4 0.0 46-47 0.028091125407596722 8.160094526534995E-5 0.0 1.2240141789802492E-4 0.0 48-49 0.0363532211157134 8.160094526534995E-5 0.0 1.2240141789802492E-4 0.0 50-51 0.04720614683600495 8.160094526534995E-5 0.0 1.2240141789802492E-4 0.0 52-53 0.05848747751893958 8.160094526534995E-5 0.0 1.2240141789802492E-4 0.0 54-55 0.07370605381092735 8.160094526534995E-5 0.0 1.2240141789802492E-4 0.0 56-57 0.09188266436878405 8.160094526534995E-5 0.0 1.2240141789802492E-4 0.0 58-59 0.1130785099014587 8.160094526534995E-5 0.0 1.2240141789802492E-4 0.0 60-61 0.13833400246108452 1.0200118158168743E-4 0.0 1.2240141789802492E-4 0.0 62-63 0.16713913613975306 1.2240141789802492E-4 0.0 1.2240141789802492E-4 0.0 64-65 0.19967751306431136 1.2240141789802492E-4 0.0 1.2240141789802492E-4 0.0 66-67 0.23921317104537337 1.2240141789802492E-4 0.0 1.4280165421436242E-4 0.0 68-69 0.28556250795609217 1.4280165421436242E-4 0.0 1.632018905306999E-4 0.0 70-71 0.33627749543850716 1.632018905306999E-4 0.0 1.632018905306999E-4 0.0 72-73 0.3954585809922022 1.632018905306999E-4 0.0 1.836021268470374E-4 0.0 74-75 0.4639217740698308 1.632018905306999E-4 0.0 2.040023631633749E-4 0.0 76-77 0.5370770215002171 1.632018905306999E-4 0.0 2.040023631633749E-4 0.0 78-79 0.6192695736187408 1.632018905306999E-4 0.0 2.040023631633749E-4 0.0 80-81 0.7085206075027173 1.632018905306999E-4 0.0 2.040023631633749E-4 0.0 82-83 0.8089305706517305 1.632018905306999E-4 0.0 2.040023631633749E-4 0.0 84-85 0.9190102458146876 1.632018905306999E-4 0.0 2.040023631633749E-4 0.0 86-87 1.042105271747468 1.632018905306999E-4 0.0 2.040023631633749E-4 0.0 88-89 1.1793376614474702 1.632018905306999E-4 0.0 2.040023631633749E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 1795 0.0 76.5472 1 TCGACGG 2110 0.0 66.40777 2 TATCTCG 2670 0.0 62.97546 2 ATATCTC 2760 0.0 61.497166 1 CGACGGC 2475 0.0 56.422386 3 ACGGCGC 2770 0.0 50.584976 5 GACGGCG 2800 0.0 49.873363 4 CGGCGCG 2855 0.0 49.245316 6 ATCTCGA 4120 0.0 40.92705 3 TCTCGAG 4115 0.0 39.476223 4 CTCGAGG 4450 0.0 36.50442 5 TCGAGGG 4450 0.0 36.397682 6 CGAGGGC 4755 0.0 34.063023 7 AGGGCGC 4845 0.0 33.52831 9 GAGGGCG 5130 0.0 31.573036 8 AGTCGAC 415 0.0 26.3497 1 GGGCGCG 4515 0.0 25.037937 8 CGCGCCG 7335 0.0 23.117851 9 GGCGCGC 7425 0.0 22.837633 7 GCGCGCC 7520 0.0 22.61229 8 >>END_MODULE