FastQCFastQC Report
Tue 31 May 2016
SRR2048261_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048261_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2564456
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT163290.6367432313129958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG73000.090.301021
TATCTCG61200.086.216162
ATCTCGA64300.082.1334153
ATATCTC67350.078.6961
TCGAGGG53650.077.9002766
TCTCGAG54000.077.5712664
TCGACGG85200.077.314852
AGGGCGC54300.077.24079
CTCGAGG54600.076.631855
CTCGACG14350.075.1276865
CGAGGGC56200.074.534677
CGACGGC88850.074.192173
GAGGGCG57750.072.7043158
ACGGCGC91000.072.073955
GACGGCG91650.071.666434
CGGCGCG92050.071.303416
TCTCGAC15950.068.484644
CGCGCCG143400.046.2735259
GCGCGCC144300.046.015148
GGCGCGC144050.045.9595267
Produced by FastQC (version 0.11.5)