##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048261_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2564456 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.40114238653344 31.0 31.0 34.0 28.0 34.0 2 31.55736421291689 31.0 31.0 34.0 28.0 34.0 3 31.579798990507147 31.0 31.0 34.0 28.0 34.0 4 35.27440829556054 37.0 35.0 37.0 32.0 37.0 5 35.15252903539776 37.0 35.0 37.0 32.0 37.0 6 35.22656812984898 37.0 35.0 37.0 32.0 37.0 7 35.16801614065517 37.0 35.0 37.0 32.0 37.0 8 35.206153663778984 37.0 35.0 37.0 32.0 37.0 9 36.72838449948059 39.0 37.0 39.0 32.0 39.0 10-11 36.680634801298986 39.0 37.0 39.0 32.0 39.0 12-13 36.714752953452894 39.0 37.0 39.0 32.0 39.0 14-15 37.86174182750649 40.0 37.0 41.0 32.0 41.0 16-17 37.79167336074396 40.0 37.0 41.0 32.0 41.0 18-19 37.794874624481764 40.0 37.0 41.0 32.0 41.0 20-21 37.83750335353775 40.0 37.0 41.0 32.0 41.0 22-23 37.70607723431402 40.0 37.0 41.0 32.0 41.0 24-25 37.76885682577513 40.0 37.0 41.0 32.0 41.0 26-27 37.73119562199547 40.0 37.0 41.0 32.0 41.0 28-29 37.51247282074639 40.0 37.0 41.0 31.5 41.0 30-31 37.34172452169193 39.5 36.5 41.0 31.0 41.0 32-33 37.327047334795374 39.0 36.0 41.0 31.0 41.0 34-35 37.09905609610772 39.0 35.0 41.0 31.0 41.0 36-37 36.95328697392351 39.0 35.0 41.0 30.0 41.0 38-39 36.832554155735174 39.0 35.0 41.0 30.0 41.0 40-41 36.525683029851166 39.0 35.0 40.5 29.5 41.0 42-43 36.34138429358897 39.0 35.0 40.0 29.0 41.0 44-45 36.33147614932757 39.0 35.0 40.0 29.0 41.0 46-47 36.25038000262044 39.0 35.0 40.0 29.0 41.0 48-49 36.244467442607714 39.0 35.0 40.0 29.0 41.0 50-51 36.31281605143547 39.0 35.0 40.0 29.0 41.0 52-53 36.22599276415738 39.0 35.0 40.0 29.0 41.0 54-55 35.95292997813182 38.0 34.0 40.0 28.0 41.0 56-57 35.778106935739984 38.0 34.0 40.0 28.0 41.0 58-59 35.54894897787289 38.0 34.0 40.0 28.0 41.0 60-61 35.355745234076934 38.0 34.0 40.0 28.0 41.0 62-63 35.24312895210524 37.5 34.0 40.0 28.0 41.0 64-65 34.995172465427366 37.0 34.0 40.0 28.0 41.0 66-67 34.75159877962422 37.0 34.0 39.5 28.0 41.0 68-69 34.57070290931098 36.0 34.0 39.0 28.0 41.0 70-71 34.25337361998022 36.0 33.0 39.0 28.0 41.0 72-73 33.91945075290822 35.0 33.0 38.5 27.0 40.0 74-75 33.58654993495696 35.0 33.0 37.5 27.0 39.5 76-77 33.23502976849671 35.0 33.0 37.0 26.5 39.0 78-79 32.91369085685229 35.0 33.0 36.5 26.0 39.0 80-81 32.599381311280055 35.0 33.0 36.0 26.0 38.0 82-83 32.333825185536426 35.0 32.0 36.0 26.0 37.0 84-85 32.098764221339735 35.0 32.0 35.0 26.0 37.0 86-87 31.879254703531664 35.0 32.0 35.0 26.0 36.0 88-89 31.673445557264387 35.0 32.0 35.0 25.0 36.0 90-91 31.49939616823217 35.0 32.0 35.0 25.0 36.0 92-93 31.325181831936284 35.0 32.0 35.0 25.0 36.0 94-95 31.149949540955276 34.5 32.0 35.0 24.5 35.0 96-97 30.983865973914156 34.0 32.0 35.0 24.0 35.0 98-99 30.755297809749905 34.0 32.0 35.0 23.5 35.0 100-101 30.047729615949738 34.0 31.0 35.0 18.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8529900583267107 1101 2 0.9023009780622857 1101 3 0.7672974164824069 1101 4 0.6719703660801812 1101 5 0.7618675208111014 1101 6 0.6395049279613758 1101 7 0.6620966098678522 1101 8 0.7069291868664536 1101 9 0.9079093473170161 1101 10-11 0.9434370533732164 1101 12-13 0.8815034978812761 1101 14-15 1.0103318081737385 1101 16-17 1.0578556388755374 1101 18-19 1.0345223398212795 1101 20-21 1.1152990702659906 1101 22-23 1.1544299654125538 1101 24-25 1.0821738409106487 1101 26-27 1.10635205669913 1101 28-29 1.177915270796305 1101 30-31 1.0975919250445543 1101 32-33 0.9844402385726596 1101 34-35 1.0659109995047729 1101 36-37 1.0944741655897872 1101 38-39 1.0731624846082468 1101 40-41 1.0757050320181492 1101 42-43 0.9806319588595187 1101 44-45 1.034916439858236 1101 46-47 1.1091991341118614 1101 48-49 1.0656431365710688 1101 50-51 0.9060818993537296 1101 52-53 0.6975939924900487 1101 54-55 0.6999252498342301 1101 56-57 0.7004210218884523 1101 58-59 0.7930020153358086 1101 60-61 0.695745657877687 1101 62-63 0.6430275116769408 1101 64-65 0.6142814329890456 1101 66-67 0.642984534744798 1101 68-69 0.641947684944391 1101 70-71 0.6078119686711574 1101 72-73 0.5438225635215304 1101 74-75 0.5418104850513856 1101 76-77 0.5120909734946082 1101 78-79 0.5250609936442814 1101 80-81 0.5996387994246604 1101 82-83 0.5705940641862455 1101 84-85 0.5191071698229663 1101 86-87 0.5419025071672117 1101 88-89 0.5296914880178427 1101 90-91 0.5092423969700697 1101 92-93 0.5524785600938813 1101 94-95 0.6210784738078878 1101 96-97 0.6150609929290951 1101 98-99 0.5584287061376791 1101 100-101 0.7963603524404448 1102 1 0.8122576645267401 1102 2 0.8226622886873756 1102 3 0.7329774833035501 1102 4 0.677981232642324 1102 5 0.7323950503190702 1102 6 0.6475992508156239 1102 7 0.6950874121236197 1102 8 0.6995603207987102 1102 9 0.9602332254411294 1102 10-11 0.9056023168129315 1102 12-13 0.79208835611729 1102 14-15 0.968961525620891 1102 16-17 1.0339814280570465 1102 18-19 1.083363811528784 1102 20-21 1.0257168114854522 1102 22-23 1.2057410374334623 1102 24-25 1.0827522283073492 1102 26-27 1.1081831267355895 1102 28-29 1.159195973068421 1102 30-31 1.1669858494695973 1102 32-33 1.0814515597615113 1102 34-35 1.0912853608675732 1102 36-37 1.1763970013656149 1102 38-39 1.1869570373847154 1102 40-41 1.0839420405144935 1102 42-43 1.1345069031538415 1102 44-45 1.0696582820244984 1102 46-47 1.110095396678247 1102 48-49 0.9215183956398647 1102 50-51 0.9696188695297039 1102 52-53 0.9520893119305356 1102 54-55 0.9524064510409787 1102 56-57 0.8739386308684303 1102 58-59 0.8608611469175216 1102 60-61 0.8416940378583746 1102 62-63 0.698923663045818 1102 64-65 0.6736806799253401 1102 66-67 0.6067194471682171 1102 68-69 0.6548116322333541 1102 70-71 0.6447777074374272 1102 72-73 0.6619949833759335 1102 74-75 0.5759181272280856 1102 76-77 0.5381068054529479 1102 78-79 0.6354186347517725 1102 80-81 0.6844656007600562 1102 82-83 0.6711625293442225 1102 84-85 0.6707827023520778 1102 86-87 0.7101919089332007 1102 88-89 0.673899573615973 1102 90-91 0.7273620193946719 1102 92-93 0.6878154906664342 1102 94-95 0.6493279321477452 1102 96-97 0.6761613478202051 1102 98-99 0.6808805191274274 1102 100-101 0.7404346909649888 1103 1 0.6827819653779272 1103 2 0.7184147710439035 1103 3 0.6894032368116356 1103 4 0.5884335227231503 1103 5 0.7096247599225975 1103 6 0.5521431158633163 1103 7 0.5300922099150753 1103 8 0.6156772382974722 1103 9 0.6882705079532059 1103 10-11 0.7833452541234323 1103 12-13 0.6943433395139991 1103 14-15 0.8634686272302119 1103 16-17 0.799963234352191 1103 18-19 0.9259060456914057 1103 20-21 0.8837853240758733 1103 22-23 0.9711925812387605 1103 24-25 0.9754617909342116 1103 26-27 0.9968299311238198 1103 28-29 1.0967782694856325 1103 30-31 1.039085145015477 1103 32-33 0.9690680328141141 1103 34-35 1.045434706340039 1103 36-37 1.1684560194469 1103 38-39 1.1395113661361194 1103 40-41 0.9982425212358521 1103 42-43 0.9174369800277162 1103 44-45 0.8878384746942132 1103 46-47 0.9808064805508394 1103 48-49 0.960863390572257 1103 50-51 0.9529419134687416 1103 52-53 0.9365806999965969 1103 54-55 0.9613977863216903 1103 56-57 0.7999037085999277 1103 58-59 0.7874620042885354 1103 60-61 0.8059072889279406 1103 62-63 0.7802433372885247 1103 64-65 0.7417061034019099 1103 66-67 0.6769881406207077 1103 68-69 0.6700138643359637 1103 70-71 0.6633771527737622 1103 72-73 0.6920790951592224 1103 74-75 0.634929623725732 1103 76-77 0.6629704254738513 1103 78-79 0.5576596155783875 1103 80-81 0.5951828347139028 1103 82-83 0.5924276772920081 1103 84-85 0.5640104285972356 1103 86-87 0.5930365549255718 1103 88-89 0.6591401280911917 1103 90-91 0.6579238652927337 1103 92-93 0.6779872453904012 1103 94-95 0.6719977912033848 1103 96-97 0.70614749086484 1103 98-99 0.7648743116568362 1103 100-101 0.8148286178025792 1104 1 0.5503521447032647 1104 2 0.622663933370994 1104 3 0.5636766701401399 1104 4 0.5267236598027338 1104 5 0.574586808756429 1104 6 0.49485355763188466 1104 7 0.48919907340792435 1104 8 0.5304320402517604 1104 9 0.653871748387914 1104 10-11 0.7066600871206603 1104 12-13 0.6031407891614222 1104 14-15 0.7427048149138429 1104 16-17 0.6837856311256019 1104 18-19 0.7211413717838866 1104 20-21 0.7952622580727535 1104 22-23 0.8807500964569144 1104 24-25 0.7357438016912781 1104 26-27 0.7832939439258269 1104 28-29 0.8492772045226218 1104 30-31 0.8999201348094701 1104 32-33 0.7229465559442545 1104 34-35 0.777462219532481 1104 36-37 0.915161796223174 1104 38-39 0.7847066592401291 1104 40-41 0.7694437300289962 1104 42-43 0.7459706662642276 1104 44-45 0.6874474983115348 1104 46-47 0.8013678830844029 1104 48-49 0.8745611399058859 1104 50-51 0.7596409984824462 1104 52-53 0.6222379064422796 1104 54-55 0.6512016407127916 1104 56-57 0.5692245962526101 1104 58-59 0.5346485243421739 1104 60-61 0.509046484537464 1104 62-63 0.4865988815367359 1104 64-65 0.5103577346259272 1104 66-67 0.5327296675505124 1104 68-69 0.4942626845921154 1104 70-71 0.4712871688778222 1104 72-73 0.47612188716353643 1104 74-75 0.49714062055782904 1104 76-77 0.4116242160206198 1104 78-79 0.4402432345498468 1104 80-81 0.5301406664545567 1104 82-83 0.453428250795298 1104 84-85 0.4416747469113389 1104 86-87 0.4043863614684291 1104 88-89 0.35317085675037774 1104 90-91 0.3245535099077621 1104 92-93 0.34058622548454665 1104 94-95 0.33617117947473574 1104 96-97 0.36023900659716546 1104 98-99 0.3562419914057564 1104 100-101 0.4162950063940869 1105 1 0.4407086850536608 1105 2 0.4811034169548769 1105 3 0.3713879052816331 1105 4 0.3623670013450564 1105 5 0.42327153519708816 1105 6 0.3500350358562514 1105 7 0.3732136250764029 1105 8 0.4108524596177432 1105 9 0.49192490411343215 1105 10-11 0.4961791563927136 1105 12-13 0.42144659338956103 1105 14-15 0.5002185395653314 1105 16-17 -1.5339233057120083 1105 18-19 0.06861174787046309 1105 20-21 0.46194626842150655 1105 22-23 0.657792674132402 1105 24-25 0.4514537461648942 1105 26-27 0.593924557014347 1105 28-29 0.6534223977424176 1105 30-31 0.6602758026556117 1105 32-33 0.6520199138673135 1105 34-35 0.677846307608597 1105 36-37 0.6878659795086506 1105 38-39 0.8110351932680544 1105 40-41 0.6864597708068629 1105 42-43 0.6412362932675961 1105 44-45 0.6616096149217299 1105 46-47 0.7081751985005411 1105 48-49 0.651617177555508 1105 50-51 0.4776680801632196 1105 52-53 0.5318876172656388 1105 54-55 0.7150812681109144 1105 56-57 0.5382537277863477 1105 58-59 0.5350645067048205 1105 60-61 0.4707886627230522 1105 62-63 0.4358338442717624 1105 64-65 0.3987370952186353 1105 66-67 0.4492769766995224 1105 68-69 0.46048900261885706 1105 70-71 0.408640618754319 1105 72-73 0.30050707757428796 1105 74-75 0.25850049291684485 1105 76-77 0.3376181393477111 1105 78-79 0.2895017655399812 1105 80-81 0.31626013463129965 1105 82-83 0.3633118353194149 1105 84-85 0.3629988306355685 1105 86-87 0.30193622056431124 1105 88-89 0.3313295748501055 1105 90-91 0.35555486282136783 1105 92-93 0.3328697765981339 1105 94-95 0.3400020317571055 1105 96-97 0.3637873590271674 1105 98-99 0.33341842400187716 1105 100-101 0.38834906760877175 1106 1 0.3084418977050518 1106 2 0.3957084963471367 1106 3 0.36970953789545646 1106 4 0.31635309438195236 1106 5 0.3862632821958911 1106 6 0.34533227039648295 1106 7 0.31243184470231 1106 8 0.35569561869076693 1106 9 0.5021580311995208 1106 10-11 0.5548486004178272 1106 12-13 0.4100586566269797 1106 14-15 0.4761715787535863 1106 16-17 0.5370289164483495 1106 18-19 0.540126683923944 1106 20-21 0.5354779759175727 1106 22-23 0.5918260925589536 1106 24-25 0.5423151628880731 1106 26-27 0.4880412801004681 1106 28-29 0.5619514824876788 1106 30-31 0.5781059428206987 1106 32-33 0.49689176816232106 1106 34-35 0.6399802825904786 1106 36-37 0.7195108170992768 1106 38-39 0.7020436964739929 1106 40-41 0.6172397765727311 1106 42-43 0.5375340731726155 1106 44-45 0.5612197912162955 1106 46-47 0.577131512130677 1106 48-49 0.6023475243326288 1106 50-51 0.5701314724309228 1106 52-53 0.45972868296616554 1106 54-55 0.495515960221212 1106 56-57 0.5193317261098045 1106 58-59 0.5140097563929444 1106 60-61 0.612879097573952 1106 62-63 0.5722281783146173 1106 64-65 0.4809779554766038 1106 66-67 0.5781109593098606 1106 68-69 0.5535110370516207 1106 70-71 0.4629713656015184 1106 72-73 0.5165996144883351 1106 74-75 0.48698001523608525 1106 76-77 0.5095598665350636 1106 78-79 0.44120971330640657 1106 80-81 0.5166511707353507 1106 82-83 0.5298671598039562 1106 84-85 0.5819237667459163 1106 86-87 0.5246343718280961 1106 88-89 0.5200495576576536 1106 90-91 0.4734710350064333 1106 92-93 0.405569810834983 1106 94-95 0.5141230990927603 1106 96-97 0.5291035155690729 1106 98-99 0.5566248975237897 1106 100-101 0.555181929447798 1107 1 0.298453301811076 1107 2 0.3754747861692884 1107 3 0.2995694075994635 1107 4 0.32425534070471684 1107 5 0.36804483091579954 1107 6 0.27877287372773196 1107 7 0.1879959248048877 1107 8 0.2791772792154248 1107 9 0.3753295292000445 1107 10-11 0.33523706792105656 1107 12-13 0.24844933040761674 1107 14-15 0.3553702723076242 1107 16-17 0.36883792030960194 1107 18-19 0.38876822720873605 1107 20-21 0.3886395321861471 1107 22-23 0.5094695754077918 1107 24-25 0.35070781975317544 1107 26-27 0.415674318046662 1107 28-29 0.4970255543652229 1107 30-31 0.5459640241311092 1107 32-33 0.4536170609742314 1107 34-35 0.43690251136695935 1107 36-37 0.5784978347474095 1107 38-39 0.6081359589037092 1107 40-41 0.5025599142639123 1107 42-43 0.47330354009561404 1107 44-45 0.4127789023164823 1107 46-47 0.46874945035206395 1107 48-49 0.5362712372981946 1107 50-51 0.4154418119965939 1107 52-53 0.30989864832412195 1107 54-55 0.34274755675330226 1107 56-57 0.3684280688262689 1107 58-59 0.3365497861425055 1107 60-61 0.4018457483196727 1107 62-63 0.2801478437707843 1107 64-65 0.2995620660478693 1107 66-67 0.28964345059782204 1107 68-69 0.292299265006136 1107 70-71 0.3254378249998595 1107 72-73 0.2430677519992983 1107 74-75 0.22960527596126212 1107 76-77 0.3415501050237211 1107 78-79 0.33772175383018066 1107 80-81 0.34008849413915243 1107 82-83 0.30741550831334763 1107 84-85 0.27704165722637697 1107 86-87 0.23082119526769773 1107 88-89 0.26712086308302574 1107 90-91 0.3546347526068736 1107 92-93 0.3491779425994892 1107 94-95 0.3876966951506198 1107 96-97 0.39690794178292776 1107 98-99 0.33887558987679256 1107 100-101 0.2556935027572962 1108 1 0.1931747470037699 1108 2 0.25886011302000966 1108 3 0.21519301633056998 1108 4 0.10960287868360297 1108 5 0.29013332031380656 1108 6 0.17445002940657872 1108 7 0.2146956126174473 1108 8 0.25211115083991587 1108 9 0.16938721676770996 1108 10-11 0.24035329340671296 1108 12-13 0.12683944316673745 1108 14-15 0.20186613632761663 1108 16-17 0.27164386139980934 1108 18-19 0.2612495707667932 1108 20-21 0.2221130627968364 1108 22-23 0.37281423618319565 1108 24-25 0.13427062922740163 1108 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-0.34401900302370336 2214 94-95 -0.4461942389249671 2214 96-97 -0.5698243747260854 2214 98-99 -0.4569039515763009 2214 100-101 -0.5974815031960823 2215 1 -1.1565438507456633 2215 2 -1.6953537094535953 2215 3 -1.387741590594949 2215 4 -0.8839413227098305 2215 5 -0.9261446189758189 2215 6 -0.6755973998475469 2215 7 -0.7949790132797787 2215 8 -0.5320570984747448 2215 9 -1.0702136702666678 2215 10-11 -2.8283452364331083 2215 12-13 -1.7629360279949893 2215 14-15 -3.6255562724855324 2215 16-17 -1.5636931256512199 2215 18-19 -1.4451441598316421 2215 20-21 -1.256594421698523 2215 22-23 -0.9471863783974115 2215 24-25 -1.1689285021447589 2215 26-27 -1.1829995238827138 2215 28-29 -1.1035057476447605 2215 30-31 -0.9910107991728978 2215 32-33 -0.9232564021789713 2215 34-35 -1.7612530517425355 2215 36-37 -0.9279166382107107 2215 38-39 -0.8445931719859772 2215 40-41 -0.8464008793119007 2215 42-43 -1.1263426264511267 2215 44-45 -1.1009263497815667 2215 46-47 -0.9645331881904013 2215 48-49 -0.776071215211303 2215 50-51 -0.775351234061354 2215 52-53 -1.0249430205866474 2215 54-55 -0.9318022602598361 2215 56-57 -0.9555414436649627 2215 58-59 -0.9516291304163715 2215 60-61 -0.8559847206476121 2215 62-63 -0.9288235890891485 2215 64-65 -0.8071133121764689 2215 66-67 -0.9138041630681357 2215 68-69 -0.7183896838237587 2215 70-71 -0.6337664801577603 2215 72-73 -0.6127633261884071 2215 74-75 -0.6248318794208458 2215 76-77 -0.6280429533952159 2215 78-79 -0.6631479303905721 2215 80-81 -0.5449818531606354 2215 82-83 -0.6546296181440354 2215 84-85 -0.6562983716453736 2215 86-87 -0.6780727606477015 2215 88-89 -0.8347855995095124 2215 90-91 -0.7459738056156162 2215 92-93 -0.7519073968201582 2215 94-95 -0.864063642834271 2215 96-97 -0.908941677895772 2215 98-99 -1.0248334146658742 2215 100-101 -1.0327867130303403 2216 1 -1.567058489184891 2216 2 -4.7213149380413135 2216 3 -2.096782469149204 2216 4 -1.4551131415451977 2216 5 -1.3113363688952546 2216 6 -1.2248425472235382 2216 7 -1.1955838869752071 2216 8 -1.1551637694383246 2216 9 -3.707543549539828 2216 10-11 -1.927498661158559 2216 12-13 -1.3921751018441242 2216 14-15 -1.580966979245737 2216 16-17 -1.4519898112408498 2216 18-19 -1.4781038886300735 2216 20-21 -1.4588087800118146 2216 22-23 -4.322147644169988 2216 24-25 -1.954945136171382 2216 26-27 -1.699049438357541 2216 28-29 -1.6381325975914578 2216 30-31 -1.7352734854070633 2216 32-33 -1.6775713951138513 2216 34-35 -1.8244797058489866 2216 36-37 -1.9316444202578609 2216 38-39 -1.7765293875589947 2216 40-41 -1.5612549907177353 2216 42-43 -1.8101163937730718 2216 44-45 -1.6022278198229642 2216 46-47 -1.3917934078289562 2216 48-49 -1.4592980552420443 2216 50-51 -1.730585312744516 2216 52-53 -1.8277439700400322 2216 54-55 -1.7680587366226703 2216 56-57 -1.6852059742680936 2216 58-59 -1.7251485533073705 2216 60-61 -1.8144027528249111 2216 62-63 -1.6794557316806902 2216 64-65 -1.8291282976743801 2216 66-67 -1.938375607575935 2216 68-69 -1.877866244968061 2216 70-71 -1.7957173837498175 2216 72-73 -1.866884647986172 2216 74-75 -1.842394288008066 2216 76-77 -1.8443829097649882 2216 78-79 -1.7808581487896404 2216 80-81 -1.86205714010379 2216 82-83 -1.8026300023875734 2216 84-85 -1.8685448948611203 2216 86-87 -1.751176642746909 2216 88-89 -1.70301330718695 2216 90-91 -1.8224754541443353 2216 92-93 -1.745722315385322 2216 94-95 -1.8189568603161135 2216 96-97 -1.881063879735187 2216 98-99 -1.833072649649388 2216 100-101 -1.965324083812959 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 27.0 11 337.0 12 1470.0 13 2948.0 14 4116.0 15 5249.0 16 6188.0 17 7499.0 18 10126.0 19 18430.0 20 18580.0 21 16225.0 22 17631.0 23 19769.0 24 22512.0 25 25893.0 26 30348.0 27 36637.0 28 43258.0 29 50866.0 30 59537.0 31 71921.0 32 90098.0 33 115102.0 34 152546.0 35 211293.0 36 325348.0 37 524524.0 38 550789.0 39 125028.0 40 161.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.888414540939674 40.12636598171308 13.963975205657652 20.02124427168959 2 23.096009446057955 28.659723543706733 31.135765246118474 17.108501764116834 3 22.564473712943407 29.075484235253015 22.94634807538129 25.41369397642229 4 24.361112064313055 26.658480395062345 26.182706975670474 22.797700564954127 5 22.81868857125588 28.51249095811781 24.674209529900114 23.994610940726197 6 22.394808099651545 25.451089821778965 27.07155045748494 25.082551621084548 7 25.07034630346553 25.790694010737557 24.42857276553 24.71038692026691 8 26.443035092042912 25.314686623595804 25.673476168044996 22.568802116316284 9 21.739269459097756 26.878800026204384 26.110722897955746 25.27120761674211 10-11 25.354480638388804 25.6430603605599 25.331746772024943 23.67071222902635 12-13 24.444638551022127 25.748384842633293 25.17161534454091 24.635361261803673 14-15 24.309587248848867 25.656885311588756 25.40204167385669 24.63148576570569 16-17 24.443072697250344 26.809330107951002 25.347691445009367 23.399905749789284 18-19 22.891047589263135 25.448648045812472 26.885054891790826 24.775249473133563 20-21 22.877678540789937 27.834811749548443 25.679071896729756 23.608437812931864 22-23 21.88366968992065 27.029318043056165 27.978122277886776 23.10888998913641 24-25 21.838803239361486 25.796211750172354 29.209762226374718 23.155222784091443 26-27 21.727856743022738 25.738573252128276 30.532672738003345 22.00089726684564 28-29 21.780393767252985 25.567124328331637 30.72498747674985 21.927494427665533 30-31 21.8231955866359 25.552226199338403 30.639122006650304 21.98545620737539 32-33 21.76389204095687 25.56440864215151 30.713555400096865 21.958143916794764 34-35 21.867623240325678 25.689816900867463 30.60082930693579 21.84173055187107 36-37 21.88098763727504 25.626721879934543 30.520424833255422 21.97186564953499 38-39 21.951045937815007 25.675847990500866 30.314902444778504 22.058203626905627 40-41 22.383959947052055 26.111373571769363 28.979894170403238 22.524772310775344 42-43 22.453226425648644 26.214707176630675 28.904069499353763 22.427996898366924 44-45 22.474766578174865 26.453992581662543 28.657910293645124 22.413330546517468 46-47 22.409294602831945 26.806464996864833 28.428758379944906 22.355482020358313 48-49 22.53430221516087 26.90955789989287 28.016553356163126 22.53958652878313 50-51 22.470731278371346 27.03736546214807 27.917854553863297 22.57404870561728 52-53 22.561982455924852 27.131958421583064 27.730876983437614 22.57518213905447 54-55 22.633962134659356 27.006097979454513 27.59669107210262 22.763248813783505 56-57 22.69818628200289 26.998513524895728 27.58304295335931 22.72025723974207 58-59 22.721661046241387 27.09596109272298 27.45211849998596 22.730259361049672 60-61 22.7712232145921 27.02136437513453 27.3310206921078 22.876391718165568 62-63 22.756444251724343 27.062289234051978 27.407274681257938 22.773991832965745 64-65 22.771671652779382 27.196411246673758 27.2597970095802 22.77212009096666 66-67 22.86157376067283 27.1410193818884 27.12317934095964 22.874227516479127 68-69 22.834539892491776 27.16830671624185 27.17487731311331 22.82227607815306 70-71 22.870382395002537 27.268024371134487 27.100329500920566 22.76126373294241 72-73 22.891722723411124 27.24089675452264 26.981624359009103 22.885756163057124 74-75 22.874816895828374 27.234748273073954 27.1078012466974 22.782633584400276 76-77 22.954995621869088 27.394621186878048 26.876517990891223 22.773865200361637 78-79 23.00894428432662 27.258697933381715 26.85073218971382 22.881625592577848 80-81 22.955102368689502 27.212301556353474 27.007111839704017 22.825484235253015 82-83 22.99914679760542 27.338683135916547 26.895060784821425 22.767109281656616 84-85 23.070871407544956 27.2731296265931 26.856686285523878 22.799312680338062 86-87 23.052647423079204 27.2885750428161 26.875582969643464 22.783194564461233 88-89 23.128009045194975 27.36614068756506 26.7660327894167 22.739817477823266 90-91 23.142822493347516 27.252894960958578 26.79246592649669 22.81181661919721 92-93 23.140509403325208 27.221990850144206 26.880002121344 22.75749762518659 94-95 23.25261536889697 27.354475442420156 26.69873703429431 22.69417215438856 96-97 23.288650145260984 27.329518201493556 26.686762727396808 22.695068925848656 98-99 23.347165374137855 27.32312960323233 26.694818249713837 22.63488677291598 100-101 23.43489689891245 27.40193762874715 26.55271841035961 22.610447061980793 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 108.0 1 105.5 2 81.0 3 54.5 4 41.5 5 32.5 6 32.5 7 38.0 8 44.5 9 42.0 10 42.5 11 47.5 12 60.0 13 83.5 14 102.0 15 131.0 16 387.5 17 564.0 18 665.0 19 1056.5 20 1569.0 21 2222.0 22 3110.0 23 4319.0 24 5806.0 25 7787.5 26 10335.0 27 13326.5 28 16847.0 29 20929.5 30 25748.5 31 31143.0 32 36503.5 33 42474.5 34 48325.5 35 54663.5 36 61790.5 37 68520.0 38 75211.5 39 81830.5 40 87781.0 41 91683.5 42 95434.5 43 99149.0 44 101869.0 45 103987.0 46 105655.0 47 105950.5 48 104396.5 49 101987.0 50 99015.0 51 96141.0 52 93131.0 53 89261.0 54 83676.5 55 76606.5 56 68755.5 57 60767.5 58 53345.0 59 45989.5 60 38070.0 61 30768.0 62 24962.0 63 19923.0 64 15710.0 65 12392.5 66 9550.5 67 7195.0 68 5526.5 69 4395.0 70 3559.0 71 2933.0 72 2418.0 73 1975.5 74 1505.5 75 1057.0 76 694.0 77 459.5 78 298.0 79 169.5 80 71.5 81 36.5 82 23.0 83 10.5 84 10.0 85 7.5 86 3.0 87 1.0 88 0.5 89 1.0 90 1.0 91 0.5 92 1.5 93 1.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 1.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 3.8994624980892634E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.00540075555985363 16-17 2.144704373949095E-4 18-19 5.8491937471338954E-5 20-21 0.0 22-23 0.015071422555115004 24-25 0.0 26-27 5.8491937471338954E-5 28-29 0.008793287933191288 30-31 0.002456661373796236 32-33 7.798924996178527E-5 34-35 9.94362937012762E-4 36-37 0.02259738517642728 38-39 4.28940874789819E-4 40-41 0.001969228561535078 42-43 1.9497312490446317E-5 44-45 0.0 46-47 0.0 48-49 0.010255586369974763 50-51 0.0011308441244458863 52-53 1.9497312490446317E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 3.8994624980892634E-5 70-71 0.010723521869745475 72-73 0.006414615809356838 74-75 1.9497312490446317E-5 76-77 2.144704373949095E-4 78-79 9.748656245223158E-4 80-81 0.0 82-83 0.0 84-85 5.8491937471338954E-5 86-87 0.0 88-89 1.9497312490446317E-5 90-91 0.0 92-93 0.001715763499159276 94-95 0.0037044893731848004 96-97 0.00413343024797462 98-99 0.01407705961810224 100-101 0.03146866235958036 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2564456.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.01739442747068 #Duplication Level Percentage of deduplicated Percentage of total 1 87.47833724550131 68.24831940741575 2 7.930261087403552 12.37396614337571 3 2.087925778058704 4.8868358688626845 4 0.8831146442149702 2.7559321408957906 5 0.48106603646596735 1.8765759356312683 6 0.3067099708551969 1.4357227662628762 7 0.2090182655430654 1.1414942325793398 8 0.14352716422368753 0.8958092305836631 9 0.09499054517222838 0.6669823346623991 >10 0.38131877807122627 4.352348023083976 >50 0.0015234648346046445 0.07528755813384418 >100 0.0015563415794199566 0.22612281572492995 >500 3.503651179248405E-4 0.19013148406987695 >1k 2.502607984898207E-4 0.23683455551148602 >5k 0.0 0.0 >10k+ 5.005215969796414E-5 0.6376375032064394 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 16329 0.6367432313129958 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.8994624980892634E-5 0.0 2 0.0 0.0 0.0 3.8994624980892634E-5 0.0 3 3.8994624980892634E-5 0.0 0.0 3.8994624980892634E-5 0.0 4 3.8994624980892634E-5 3.8994624980892634E-5 0.0 3.8994624980892634E-5 0.0 5 7.798924996178527E-5 3.8994624980892634E-5 0.0 3.8994624980892634E-5 0.0 6 7.798924996178527E-5 3.8994624980892634E-5 0.0 3.8994624980892634E-5 0.0 7 7.798924996178527E-5 3.8994624980892634E-5 0.0 3.8994624980892634E-5 0.0 8 1.5597849992357053E-4 3.8994624980892634E-5 0.0 3.8994624980892634E-5 0.0 9 2.3396774988535581E-4 3.8994624980892634E-5 0.0 3.8994624980892634E-5 0.0 10-11 4.28940874789819E-4 3.8994624980892634E-5 0.0 3.8994624980892634E-5 0.0 12-13 9.943629370127622E-4 3.8994624980892634E-5 0.0 3.8994624980892634E-5 1.9497312490446317E-5 14-15 0.0011503414369363326 3.8994624980892634E-5 0.0 3.8994624980892634E-5 3.8994624980892634E-5 16-17 0.0014233038118025812 3.8994624980892634E-5 0.0 3.8994624980892634E-5 3.8994624980892634E-5 18-19 0.0016962661866688296 5.8491937471338954E-5 0.0 3.8994624980892634E-5 3.8994624980892634E-5 20-21 0.0022616882488917726 7.798924996178527E-5 0.0 3.8994624980892634E-5 3.8994624980892634E-5 22-23 0.0027296237486624845 7.798924996178527E-5 0.0 3.8994624980892634E-5 3.8994624980892634E-5 24-25 0.0028271103111147163 7.798924996178527E-5 0.0 3.8994624980892634E-5 3.8994624980892634E-5 26-27 0.0031000726859809645 7.798924996178527E-5 0.0 3.8994624980892634E-5 3.8994624980892634E-5 28-29 0.003743483998165693 7.798924996178527E-5 0.0 3.8994624980892634E-5 3.8994624980892634E-5 30-31 0.004698852310197562 1.1698387494267791E-4 0.0 3.8994624980892634E-5 3.8994624980892634E-5 32-33 0.006063664184528805 1.1698387494267791E-4 0.0 7.798924996178527E-5 3.8994624980892634E-5 34-35 0.007876914246140312 1.5597849992357053E-4 0.0 7.798924996178527E-5 3.8994624980892634E-5 36-37 0.010138602495032085 1.7547581241401683E-4 0.0 7.798924996178527E-5 3.8994624980892634E-5 38-39 0.014057562305611794 1.9497312490446316E-4 0.0 9.748656245223158E-5 3.8994624980892634E-5 40-41 0.01926334474056096 1.9497312490446316E-4 0.0 1.1698387494267791E-4 3.8994624980892634E-5 42-43 0.025697457862408245 1.9497312490446316E-4 0.0 1.1698387494267791E-4 3.8994624980892634E-5 44-45 0.03396431835835748 1.9497312490446316E-4 0.0 1.5597849992357053E-4 3.8994624980892634E-5 46-47 0.04425889935331314 1.9497312490446316E-4 0.0 1.5597849992357053E-4 3.8994624980892634E-5 48-49 0.05634723309738986 1.9497312490446316E-4 0.0 1.5597849992357053E-4 3.8994624980892634E-5 50-51 0.07188659115227558 1.9497312490446316E-4 0.0 1.5597849992357053E-4 3.8994624980892634E-5 52-53 0.09050652458065181 2.3396774988535581E-4 0.0 1.5597849992357053E-4 3.8994624980892634E-5 54-55 0.11347435869439756 2.3396774988535581E-4 0.0 1.5597849992357053E-4 3.8994624980892634E-5 56-57 0.13854790255711152 2.3396774988535581E-4 0.0 1.9497312490446316E-4 3.8994624980892634E-5 58-59 0.16744291966795297 2.3396774988535581E-4 0.0 1.9497312490446316E-4 3.8994624980892634E-5 60-61 0.20271355796317037 2.5346506237580214E-4 0.0 1.9497312490446316E-4 3.8994624980892634E-5 62-63 0.243150984068356 2.729623748662484E-4 0.0 1.9497312490446316E-4 3.8994624980892634E-5 64-65 0.28955458779561827 2.729623748662484E-4 0.0 1.9497312490446316E-4 3.8994624980892634E-5 66-67 0.34434203589377244 2.729623748662484E-4 0.0 1.9497312490446316E-4 3.8994624980892634E-5 68-69 0.40763031223776114 2.729623748662484E-4 0.0 1.9497312490446316E-4 3.8994624980892634E-5 70-71 0.4748765430173105 2.729623748662484E-4 0.0 1.9497312490446316E-4 7.798924996178527E-5 72-73 0.5544840699158028 2.729623748662484E-4 0.0 1.9497312490446316E-4 7.798924996178527E-5 74-75 0.6445421563091744 2.729623748662484E-4 0.0 1.9497312490446316E-4 7.798924996178527E-5 76-77 0.7444658828227118 2.729623748662484E-4 0.0 1.9497312490446316E-4 7.798924996178527E-5 78-79 0.8555030774558035 2.729623748662484E-4 0.0 1.9497312490446316E-4 7.798924996178527E-5 80-81 0.9784141353955771 2.729623748662484E-4 0.0 1.9497312490446316E-4 7.798924996178527E-5 82-83 1.1163186266405039 2.729623748662484E-4 0.0 1.9497312490446316E-4 7.798924996178527E-5 84-85 1.265726532254794 2.729623748662484E-4 0.0 1.9497312490446316E-4 7.798924996178527E-5 86-87 1.4316681588609825 2.729623748662484E-4 0.0 1.9497312490446316E-4 7.798924996178527E-5 88-89 1.6107314767732417 2.729623748662484E-4 0.0 1.9497312490446316E-4 7.798924996178527E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 7300 0.0 90.30102 1 TATCTCG 6120 0.0 86.21616 2 ATCTCGA 6430 0.0 82.133415 3 ATATCTC 6735 0.0 78.696 1 TCGAGGG 5365 0.0 77.900276 6 TCTCGAG 5400 0.0 77.571266 4 TCGACGG 8520 0.0 77.31485 2 AGGGCGC 5430 0.0 77.2407 9 CTCGAGG 5460 0.0 76.63185 5 CTCGACG 1435 0.0 75.127686 5 CGAGGGC 5620 0.0 74.53467 7 CGACGGC 8885 0.0 74.19217 3 GAGGGCG 5775 0.0 72.704315 8 ACGGCGC 9100 0.0 72.07395 5 GACGGCG 9165 0.0 71.66643 4 CGGCGCG 9205 0.0 71.30341 6 TCTCGAC 1595 0.0 68.48464 4 CGCGCCG 14340 0.0 46.273525 9 GCGCGCC 14430 0.0 46.01514 8 GGCGCGC 14405 0.0 45.959526 7 >>END_MODULE