FastQCFastQC Report
Tue 31 May 2016
SRR2048261_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048261_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2564456
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT80820.31515455909557427No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT77090.30060956397770133No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT31100.12127328369057608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG16200.067.204761
TCGACGG18400.059.888452
TATCTCG20250.055.82452
ATATCTC22150.051.5130271
CGACGGC21700.050.9998743
ACGGCGC23800.046.3003165
GACGGCG25250.043.4533774
CGGCGCG26600.041.6043476
ATCTCGA34100.035.691531
TCTCGAG36750.031.2774242
TCGAGGG38000.030.4985494
CTCGAGG38250.030.1750353
GGGCGCG38900.029.5481458
CGAGGGC41000.028.2669495
AGGGCGC41150.028.0479357
GGTCGAC2305.456968E-1226.8716961
AGTCGAC4900.026.1967411
GAGGGCG44600.026.09136
CGCGCCG64200.021.2339
GCGCGCC65800.020.7888778
Produced by FastQC (version 0.11.5)