FastQCFastQC Report
Tue 31 May 2016
SRR2048260_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048260_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3337225
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT85820.25715976597322626No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT34050.10203087894882724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGG195100.084.95282
CGACGGC195650.084.685883
ATATCTC44500.083.637971
GTCGACG198200.083.459791
CGGCGCG200950.082.335526
ATCTCGA47100.078.688753
TCTCGAG35200.077.747464
GACGGCG216400.076.5378954
ACGGCGC216900.076.329265
AGGGCGC36100.076.3070149
CTCGAGG36400.075.174225
TCGAGGG37250.073.709476
CGAGGGC38400.071.622517
CGCGCCG234250.070.861659
TATCTCG53100.069.979482
GCGCGCC238100.069.836578
GGCGCGC239600.069.4275057
GAGGGCG39800.069.214188
CTCGACG18000.053.8400545
TCTCGAC21650.044.9885864
Produced by FastQC (version 0.11.5)