##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2048260_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3337225
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.04925679269453	31.0	31.0	34.0	28.0	34.0
2	31.446952482976126	31.0	31.0	34.0	28.0	34.0
3	31.44826525032025	31.0	31.0	34.0	28.0	34.0
4	35.07556098255287	37.0	35.0	37.0	32.0	37.0
5	35.05849710463034	37.0	35.0	37.0	32.0	37.0
6	35.16362217111522	37.0	35.0	37.0	32.0	37.0
7	35.08137030017455	37.0	35.0	37.0	32.0	37.0
8	35.118093625692005	37.0	35.0	37.0	32.0	37.0
9	36.70024796050611	39.0	37.0	39.0	32.0	39.0
10-11	36.644902726067315	39.0	37.0	39.0	32.0	39.0
12-13	36.621101513982424	39.0	37.0	39.0	32.0	39.0
14-15	37.82934264246492	40.0	37.0	41.0	32.0	41.0
16-17	37.80333360801265	40.0	37.5	41.0	32.0	41.0
18-19	37.80781053120482	40.0	37.5	41.0	32.0	41.0
20-21	37.7995154656938	40.0	37.0	41.0	32.0	41.0
22-23	37.84600079407292	40.0	37.5	41.0	32.0	41.0
24-25	37.82573455490714	40.0	37.0	41.0	32.0	41.0
26-27	37.82719699750541	40.0	37.0	41.0	32.0	41.0
28-29	37.60915551094098	40.0	37.0	41.0	31.5	41.0
30-31	37.37926540763658	39.5	36.5	41.0	31.0	41.0
32-33	37.27764999363244	40.0	35.5	41.0	31.0	41.0
34-35	37.02390339278892	39.0	35.0	41.0	30.5	41.0
36-37	36.930135516784155	39.0	35.0	41.0	30.0	41.0
38-39	36.7255286952483	39.0	35.0	41.0	30.0	41.0
40-41	36.13541849890253	39.0	35.0	40.5	27.5	41.0
42-43	35.9321251938362	38.5	34.5	40.0	27.5	41.0
44-45	35.762456082523656	38.0	34.0	40.0	27.0	41.0
46-47	35.64989774438344	38.0	34.0	40.0	27.0	41.0
48-49	35.513476016750445	38.0	33.5	40.0	27.0	41.0
50-51	35.439446096682126	38.0	33.0	40.0	27.0	41.0
52-53	35.1663203709669	38.0	33.0	40.0	26.5	41.0
54-55	35.00526305538284	37.0	33.0	40.0	26.0	41.0
56-57	34.854625025282985	37.0	33.0	40.0	26.5	41.0
58-59	34.70842691158073	37.0	33.0	40.0	26.0	41.0
60-61	34.42210144656114	36.0	33.0	40.0	26.0	41.0
62-63	34.052813640075215	36.0	33.0	39.0	26.0	41.0
64-65	33.69584669897894	35.0	32.0	39.0	25.5	41.0
66-67	33.35994905947307	35.0	32.0	38.5	25.0	40.5
68-69	33.019487748054146	35.0	32.0	38.0	25.0	40.0
70-71	32.66856924540599	35.0	31.5	37.0	24.0	40.0
72-73	32.449334851560806	35.0	31.5	37.0	24.5	39.5
74-75	32.50820666871428	35.0	32.0	36.0	25.0	39.0
76-77	32.30379806575823	35.0	32.0	36.0	25.0	39.0
78-79	32.05985631774902	35.0	32.0	36.0	25.0	38.0
80-81	31.82048768063286	35.0	32.0	35.0	24.5	37.0
82-83	31.581409404520222	35.0	32.0	35.0	24.0	37.0
84-85	31.38848399493591	35.0	32.0	35.0	24.0	36.0
86-87	31.201887046872777	35.0	31.5	35.0	23.5	36.0
88-89	31.023411816703998	35.0	31.0	35.0	23.0	36.0
90-91	30.840431945703394	34.5	31.0	35.0	22.0	36.0
92-93	30.672598041786216	34.0	31.0	35.0	20.0	35.0
94-95	30.528317689097978	34.0	31.0	35.0	19.5	35.0
96-97	30.359456284787512	34.0	31.0	35.0	18.5	35.0
98-99	30.15404535205148	34.0	31.0	35.0	12.0	35.0
100-101	29.508402490092816	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5254629009576135
1101	2	0.5328349659217757
1101	3	0.5694512790148174
1101	4	0.4610713825645547
1101	5	0.49974403935585343
1101	6	0.5132106818729696
1101	7	0.48590217770649247
1101	8	0.5226112057260508
1101	9	0.6441845692389307
1101	10-11	0.6469863260819437
1101	12-13	0.6448277791195878
1101	14-15	0.8075456016798839
1101	16-17	0.8153447290136526
1101	18-19	0.8809780137980923
1101	20-21	0.8667514631351949
1101	22-23	0.8197487655473665
1101	24-25	0.8457951127670995
1101	26-27	0.7626473833034808
1101	28-29	0.9098968594592378
1101	30-31	0.8992778984410279
1101	32-33	0.8408608998736611
1101	34-35	0.9451482384968415
1101	36-37	0.9422458469195547
1101	38-39	0.8886397128004617
1101	40-41	0.8190504465897916
1101	42-43	0.8134645085110677
1101	44-45	0.7649271558993505
1101	46-47	0.7029126966033843
1101	48-49	0.6687273458492697
1101	50-51	0.6488712526521923
1101	52-53	0.736847349744501
1101	54-55	0.6284161373220911
1101	56-57	0.5586043879281846
1101	58-59	0.6854010141230091
1101	60-61	0.6556493219767603
1101	62-63	0.6072703046748202
1101	64-65	0.530057767896345
1101	66-67	0.6257840784835125
1101	68-69	0.5785565228232343
1101	70-71	0.6295046849826491
1101	72-73	0.639734854732076
1101	74-75	0.6207916200065924
1101	76-77	0.6454729833060568
1101	78-79	0.5547227424592833
1101	80-81	0.5021300724233839
1101	82-83	0.5100495398589615
1101	84-85	0.4687544054073456
1101	86-87	0.5393868748363992
1101	88-89	0.5069679478497591
1101	90-91	0.5179532836105913
1101	92-93	0.5413749789884683
1101	94-95	0.45218506580929585
1101	96-97	0.4821446017658779
1101	98-99	0.5475260325317919
1101	100-101	0.5366317880952636
1102	1	0.42236249643753965
1102	2	0.39160249878903386
1102	3	0.4589931160635814
1102	4	0.40516187194590003
1102	5	0.335962326301285
1102	6	0.4925411351992679
1102	7	0.5189557546312571
1102	8	0.584575326554436
1102	9	0.6309574224245296
1102	10-11	0.6050159945513656
1102	12-13	0.6786049303309554
1102	14-15	0.8223974242186856
1102	16-17	0.7221006539136212
1102	18-19	0.7814355721677018
1102	20-21	0.8114778624668517
1102	22-23	0.7400390746534597
1102	24-25	0.6721916963312751
1102	26-27	0.7331990365594336
1102	28-29	0.8593917589887639
1102	30-31	0.8343840299979917
1102	32-33	0.8184559291134264
1102	34-35	0.8804368437819861
1102	36-37	0.9091502240905598
1102	38-39	0.9230552472506233
1102	40-41	0.8351155545351006
1102	42-43	0.7974414464700033
1102	44-45	0.7346607002866321
1102	46-47	0.7311153303808098
1102	48-49	0.6617543211823431
1102	50-51	0.7125155607909619
1102	52-53	0.7137699193263458
1102	54-55	0.6520868821666568
1102	56-57	0.5761727056523114
1102	58-59	0.5561796602998612
1102	60-61	0.49487485290935496
1102	62-63	0.49848133664070815
1102	64-65	0.4783762065368009
1102	66-67	0.447944468493624
1102	68-69	0.4377810644404363
1102	70-71	0.4290254166363425
1102	72-73	0.5270842589443561
1102	74-75	0.4783697759226939
1102	76-77	0.48711106975024165
1102	78-79	0.4918558713340957
1102	80-81	0.43067539406081323
1102	82-83	0.500468569890181
1102	84-85	0.4540737893936253
1102	86-87	0.5024560059976331
1102	88-89	0.49044308305619566
1102	90-91	0.4585525888913402
1102	92-93	0.5727071471281171
1102	94-95	0.5432369828826822
1102	96-97	0.5200397783037154
1102	98-99	0.5073768657552051
1102	100-101	0.48236124331225483
1103	1	0.280225340524634
1103	2	0.3417148700847008
1103	3	0.44896396459219545
1103	4	0.30257439886541704
1103	5	0.3321890613898262
1103	6	0.35935955022534216
1103	7	0.3758868822801418
1103	8	0.47540930609827825
1103	9	0.5035703990425517
1103	10-11	0.49964350530584056
1103	12-13	0.5139665824396573
1103	14-15	0.5888648589425358
1103	16-17	0.5406410110484785
1103	18-19	0.616641760428827
1103	20-21	0.65027465228507
1103	22-23	0.5448391476121444
1103	24-25	0.5475070239466646
1103	26-27	0.6114823103560738
1103	28-29	0.7103819277003751
1103	30-31	0.6036079480709446
1103	32-33	0.6590006872351424
1103	34-35	0.6930178423132105
1103	36-37	0.75868441920381
1103	38-39	0.7458397940908412
1103	40-41	0.662451218848382
1103	42-43	0.7004906711773913
1103	44-45	0.716576494880222
1103	46-47	0.6626541718098267
1103	48-49	0.5622278592621797
1103	50-51	0.5523640648714192
1103	52-53	0.5879319345218121
1103	54-55	0.42634670892179827
1103	56-57	0.3987455229985599
1103	58-59	0.4501426497709815
1103	60-61	0.40957773296379685
1103	62-63	0.3976988974956015
1103	64-65	0.32180117909729944
1103	66-67	0.40912730571150746
1103	68-69	0.42005231925502073
1103	70-71	0.46355465717277866
1103	72-73	0.3837284584628762
1103	74-75	0.30748780791571306
1103	76-77	0.4461136798362517
1103	78-79	0.4624006721217171
1103	80-81	0.46815791437776966
1103	82-83	0.4444653187491312
1103	84-85	0.4351282662724465
1103	86-87	0.3849520034035443
1103	88-89	0.41182290870202465
1103	90-91	0.418064523074932
1103	92-93	0.42612287185631104
1103	94-95	0.44023671728749747
1103	96-97	0.3286491155210669
1103	98-99	0.3608581892938716
1103	100-101	0.5247687409896287
1104	1	0.32854256492668554
1104	2	0.30952778923092694
1104	3	0.31854581009176286
1104	4	0.32988595867219317
1104	5	0.3288837756046519
1104	6	0.3128446937011162
1104	7	0.32504902965442994
1104	8	0.3431977274691391
1104	9	0.4493522509168457
1104	10-11	0.4138221043937875
1104	12-13	0.39770290466844216
1104	14-15	0.4970023051539769
1104	16-17	0.553358341391629
1104	18-19	0.5464720124872287
1104	20-21	0.562775433482912
1104	22-23	0.4998102048302755
1104	24-25	0.4792057098485998
1104	26-27	0.4981336138946162
1104	28-29	0.6443742803900179
1104	30-31	0.5328639415054255
1104	32-33	0.509821601945859
1104	34-35	0.49974971762159726
1104	36-37	0.6141377542910575
1104	38-39	0.6018234419730106
1104	40-41	0.3244901823834354
1104	42-43	0.39347125249458514
1104	44-45	0.3476328208455115
1104	46-47	0.28688868073947305
1104	48-49	0.3202463352075853
1104	50-51	0.23378729802274023
1104	52-53	0.32701084393207935
1104	54-55	0.3273292294499086
1104	56-57	0.20945567360908512
1104	58-59	0.21662036654886663
1104	60-61	0.20518443089702743
1104	62-63	0.15828467110450362
1104	64-65	0.18879640401861053
1104	66-67	0.18795376363279104
1104	68-69	0.1994358844130275
1104	70-71	0.30019801807323176
1104	72-73	0.20516624968220043
1104	74-75	0.30133890678595066
1104	76-77	0.342512989010757
1104	78-79	0.3349577312167824
1104	80-81	0.24831761661202734
1104	82-83	0.19961115817225306
1104	84-85	0.32129005597037974
1104	86-87	0.2906242137469839
1104	88-89	0.2506978638268542
1104	90-91	0.21646323496894837
1104	92-93	0.26620061401531103
1104	94-95	0.2998062564827251
1104	96-97	0.3288448290434367
1104	98-99	0.29006975842717253
1104	100-101	0.27918829645659216
1105	1	0.21522933325760363
1105	2	0.2219315889110156
1105	3	0.2704686434550041
1105	4	0.22625195143639587
1105	5	0.1709022901281969
1105	6	0.27461075644419
1105	7	0.2691542816800734
1105	8	0.2429531680143242
1105	9	0.3038921435438269
1105	10-11	0.3481415141079367
1105	12-13	0.2971041986359424
1105	14-15	0.31315708564707023
1105	16-17	0.4207245095612109
1105	18-19	0.4625036609697162
1105	20-21	0.37501422013956187
1105	22-23	0.3474746098000523
1105	24-25	0.31417558576166726
1105	26-27	0.3422927705411567
1105	28-29	0.414468341295823
1105	30-31	0.4398013617413383
1105	32-33	0.31206242858357314
1105	34-35	0.4215830685810005
1105	36-37	0.5753587956784756
1105	38-39	0.3916984642652537
1105	40-41	0.3204240217469305
1105	42-43	0.39687640794645773
1105	44-45	0.31107608526976094
1105	46-47	0.4121075938016432
1105	48-49	0.4015613183644504
1105	50-51	0.28851251184043036
1105	52-53	0.271570903595574
1105	54-55	0.3114744892982202
1105	56-57	0.13178338547798063
1105	58-59	0.197134661720753
1105	60-61	0.0778588937249296
1105	62-63	0.10458707254527866
1105	64-65	0.04271689754372687
1105	66-67	0.12137039466546895
1105	68-69	0.14586436878040843
1105	70-71	0.1996335866551746
1105	72-73	0.2313549143890441
1105	74-75	0.21168327868348058
1105	76-77	0.2161318732602524
1105	78-79	0.1739721032774355
1105	80-81	0.22103720462749266
1105	82-83	0.24211516252861287
1105	84-85	0.16243649233607727
1105	86-87	0.12114955194303434
1105	88-89	0.09662119169763272
1105	90-91	0.1797674947536656
1105	92-93	0.3069856639783488
1105	94-95	0.2584563718708708
1105	96-97	0.27663698484886723
1105	98-99	0.23670654813848557
1105	100-101	0.27492677051776226
1106	1	0.22271887188180983
1106	2	0.25819470483291695
1106	3	0.27138422664870276
1106	4	0.17293164068649247
1106	5	0.14503961864676285
1106	6	0.20675132866128365
1106	7	0.23114064380943944
1106	8	0.2742756472035808
1106	9	0.3013073996795228
1106	10-11	0.296017070778575
1106	12-13	0.3776450540046241
1106	14-15	0.3838198933280026
1106	16-17	0.4290133241775749
1106	18-19	0.4302687347593732
1106	20-21	0.4181351599160905
1106	22-23	0.2875810604591038
1106	24-25	0.17084962990105623
1106	26-27	0.2318075598351541
1106	28-29	0.3850188739971401
1106	30-31	0.3604018043897028
1106	32-33	0.3560175902756768
1106	34-35	0.41121058087928475
1106	36-37	0.38793027801286684
1106	38-39	0.4228212140614005
1106	40-41	0.40705908774095434
1106	42-43	0.392797322633605
1106	44-45	0.3257059720987243
1106	46-47	0.3699379895866244
1106	48-49	0.39111831158135146
1106	50-51	0.36593511558054104
1106	52-53	0.31764396745700907
1106	54-55	0.187600420069451
1106	56-57	0.20179512372405384
1106	58-59	0.13901564845025405
1106	60-61	0.11633301134035179
1106	62-63	0.134679813649754
1106	64-65	0.051772461561974126
1106	66-67	0.1224004929620719
1106	68-69	0.1625279291427688
1106	70-71	0.21576785068506865
1106	72-73	0.07186844025351036
1106	74-75	0.09539776152861634
1106	76-77	0.17185556749237918
1106	78-79	0.2438276601272804
1106	80-81	0.2251086735211416
1106	82-83	0.23827532238380655
1106	84-85	0.24659541564321685
1106	86-87	0.19157553378907366
1106	88-89	0.24510991491696288
1106	90-91	0.1199366688650052
1106	92-93	0.19535843841585532
1106	94-95	0.1660926833734564
1106	96-97	0.23014044807948153
1106	98-99	0.14079298105575333
1106	100-101	0.2475018370494233
1107	1	0.12625522012241674
1107	2	0.07370531853709394
1107	3	0.14448590136487738
1107	4	0.14571503868472035
1107	5	0.1300585700898509
1107	6	0.11871430390343818
1107	7	0.1849740323991469
1107	8	-0.208820561611752
1107	9	-0.1043533022855172
1107	10-11	-0.07870412347595845
1107	12-13	-0.017373270203940194
1107	14-15	-0.25290235826472696
1107	16-17	-0.1674825853572912
1107	18-19	-0.0779565499602981
1107	20-21	-0.11060418829763563
1107	22-23	-0.12654264810981886
1107	24-25	-0.10941022440132286
1107	26-27	0.023213192401179583
1107	28-29	-0.0927224544855747
1107	30-31	-0.005069203934596089
1107	32-33	0.025581124652610754
1107	34-35	0.08073934854265019
1107	36-37	0.2698937908846162
1107	38-39	0.20435426484051789
1107	40-41	-0.17516562350714793
1107	42-43	-0.0920018279116448
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2216	62-63	-0.9404854555909807
2216	64-65	-0.8103067805613122
2216	66-67	-0.8577192488551901
2216	68-69	-0.9706498662410254
2216	70-71	-1.1722835004937657
2216	72-73	-0.9016638870332834
2216	74-75	-0.7856022272068657
2216	76-77	-0.94679888593447
2216	78-79	-0.9575999296790769
2216	80-81	-1.0109247896044629
2216	82-83	-1.300457006404919
2216	84-85	-0.9510908900832895
2216	86-87	-1.161967457240614
2216	88-89	-1.029735930165888
2216	90-91	-1.2442850093227555
2216	92-93	-1.5179987256293082
2216	94-95	-1.1491168929680526
2216	96-97	-1.0635475548342725
2216	98-99	-1.0058278515422572
2216	100-101	-1.1135351368506896
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	26.0
11	597.0
12	2481.0
13	5113.0
14	7419.0
15	9112.0
16	10482.0
17	12850.0
18	19207.0
19	26490.0
20	24172.0
21	22833.0
22	25819.0
23	30626.0
24	35978.0
25	41635.0
26	50112.0
27	59401.0
28	69311.0
29	80365.0
30	92029.0
31	106538.0
32	126897.0
33	155425.0
34	197800.0
35	280845.0
36	493698.0
37	702625.0
38	533664.0
39	113583.0
40	92.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.138984465978073	37.639795550834435	13.027801211783869	20.19341877140363
2	22.989407163916905	27.542752818335934	33.05723309185804	16.41060692588912
3	20.805020427265177	27.412019752402415	23.70420204749343	28.07875777283898
4	26.574197306811403	26.137415401239405	25.078980830227422	22.209406461721766
5	22.615351778106334	30.914480381893856	24.311408731229367	22.15875910877044
6	21.77269455062251	25.297140414992576	25.74162974826757	27.188535286117343
7	27.414085685633122	25.772016489026594	23.853072151180182	22.9608256741601
8	27.655885788744662	24.842170752275962	25.390684702924986	22.11125875605439
9	20.960617297823724	25.479836685769936	26.207978424542084	27.351567591864256
10-11	25.389083429771407	25.58066607894877	24.56497988434623	24.465270606933593
12-13	24.731789187784877	25.62684903873209	24.797248117962116	24.844113655520918
14-15	24.770468457251674	25.41742235766602	24.89749196738861	24.914617217693692
16-17	25.031732961287585	27.801430170704755	24.756069715428154	22.41076715257951
18-19	21.93607593277223	25.065501005719682	27.943245197079854	25.05517786442824
20-21	21.994348575059682	28.449031617743238	24.954566696868135	24.602053110328946
22-23	21.530660899706866	28.101431680137523	28.23233911018312	22.13556830997249
24-25	21.366421800937214	25.385839227199142	31.06280198304227	22.184936988821374
26-27	21.4646379888315	25.173874684361408	31.688286624116017	21.673200702691073
28-29	21.552821929878455	25.130543196963405	31.775051811181026	21.541583061977114
30-31	21.500931840291308	25.147686835139556	31.873837259737805	21.477544064831335
32-33	21.485940525699736	25.11120640520126	31.83176217951369	21.571090889585314
34-35	21.7183711525032	25.357614432498206	31.668828397061805	21.255186017936794
36-37	21.51113042240543	25.341853609568084	31.548655097370126	21.598360870656364
38-39	21.832391446238713	25.39251788626511	31.15239871042681	21.622691957069367
40-41	22.47705982156074	26.023116076613697	28.823447975792575	22.67637612603299
42-43	22.556795963040486	26.375471395630722	28.713383965132085	22.35434867619671
44-45	22.832603994629558	26.565184031730016	28.192992505143977	22.40921946849645
46-47	22.744271063533326	27.27622500730397	27.894178546546904	22.085325382615796
48-49	22.529691585074428	27.435818681689128	27.70052963165504	22.333960101581404
50-51	22.639194979725712	27.12902502271053	27.638193364105483	22.593586633458276
52-53	22.687935168466787	27.3607067605614	27.48501973919895	22.466338331772867
54-55	22.65019527205789	27.082045428734013	27.53120262596194	22.736556673246156
56-57	22.661223022121675	27.14156222610103	27.604132175684885	22.593082576092414
58-59	22.876453632541832	27.222255684700748	27.503999765063437	22.39729091769399
60-61	22.660533826757263	27.38110256275798	27.457842968334468	22.500520642150292
62-63	22.638344732524775	27.018900433743603	27.747484811482593	22.595270022249025
64-65	22.78956900715944	27.164448394609504	27.524323281188757	22.521659317042296
66-67	22.79565787575237	26.724817958210355	27.676045396542538	22.80347876949474
68-69	23.01984433174269	26.905243128647307	27.12617519046513	22.948737349144874
70-71	23.185133711376167	26.984694898819008	27.06249992508663	22.7676714647182
72-73	22.958931103549045	27.149680383467924	26.841361004265952	23.050027508717086
74-75	22.96904758016268	27.200689466384244	26.970697528837768	22.85956542461531
76-77	22.936022016404426	27.375081956209314	27.073746804157324	22.615149223228936
78-79	23.193762593751295	27.23753195211404	26.79986234053159	22.768843113603072
80-81	23.124186441775002	27.337129538845833	26.7048764350388	22.833807584340367
82-83	23.260142191143586	27.422196705140063	26.5298163309459	22.78784477277045
84-85	23.31198825371379	27.351332319516963	26.73420281820974	22.602476608559506
86-87	23.277498520477344	27.17025372877166	26.87482863756564	22.67741911318536
88-89	23.311508813460286	27.267100659979477	26.819827850983973	22.601562675576265
90-91	23.32869375004682	27.18994074418117	26.88503172546052	22.596333780311486
92-93	23.221583800912434	27.064267467731423	27.00274929020369	22.711399441152455
94-95	23.380638105012398	27.452958670751897	26.671875585254217	22.49452763898149
96-97	23.582377574182143	27.411576983871328	26.53787203439984	22.46817340754669
98-99	23.597839522357646	27.489456060049893	26.43769898643334	22.475005431159122
100-101	23.782468967480465	27.665770213275998	25.99764774625625	22.554113072987285
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1634.0
1	1249.5
2	691.5
3	435.5
4	310.5
5	235.5
6	169.5
7	119.5
8	95.5
9	80.0
10	65.5
11	54.0
12	57.5
13	89.0
14	120.5
15	261.0
16	1706.0
17	1895.5
18	888.5
19	1161.0
20	1639.0
21	2333.0
22	3216.5
23	4294.5
24	5622.0
25	7300.0
26	9718.5
27	13130.0
28	17002.5
29	22067.5
30	29774.5
31	38490.0
32	46413.5
33	55286.0
34	65329.0
35	76370.0
36	85026.0
37	91114.5
38	100532.0
39	113991.0
40	118895.0
41	115171.5
42	112260.0
43	114912.0
44	122860.5
45	131256.5
46	139678.5
47	143738.0
48	140630.5
49	135135.5
50	131366.0
51	131750.0
52	138496.5
53	138437.0
54	121141.0
55	106404.5
56	96895.0
57	77507.5
58	62343.0
59	57320.0
60	52452.5
61	43301.5
62	31417.5
63	22615.5
64	15948.5
65	9728.0
66	5992.0
67	4038.5
68	2928.0
69	2261.5
70	1970.5
71	1762.5
72	1719.5
73	1528.0
74	1145.5
75	948.0
76	774.0
77	531.0
78	337.5
79	215.5
80	100.5
81	49.5
82	38.5
83	20.0
84	4.0
85	1.5
86	0.5
87	0.5
88	1.5
89	2.0
90	1.5
91	2.0
92	2.0
93	1.5
94	2.0
95	1.5
96	1.0
97	1.5
98	1.5
99	2.0
100	12.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08396197439489396
2	0.04434822344912315
3	0.05189940744181168
4	0.05759276045217209
5	0.04282000764107904
6	0.017589464300429247
7	0.021065406138333496
8	0.011716321195004832
9	0.0029965015844002126
10-11	6.891953644120489E-4
12-13	5.393702851920382E-4
14-15	0.001423338252590101
16-17	2.9965015844002127E-5
18-19	0.001872813490250133
20-21	0.005708335518282405
22-23	0.0154619481755051
24-25	0.01901280255301935
26-27	0.010158140371116722
28-29	0.017679359347961256
30-31	3.4459768220602445E-4
32-33	0.005603457962828398
34-35	1.947726029860138E-4
36-37	0.0026219388863501863
38-39	0.009199259864108652
40-41	0.0020076560615481425
42-43	0.0017979009506401277
44-45	0.002082568601158148
46-47	0.0
48-49	0.0
50-51	0.0034909243458262477
52-53	0.0025020788229741775
54-55	0.002576991362584183
56-57	0.0
58-59	0.004614612439976328
60-61	0.0
62-63	0.0
64-65	0.002786746473492198
66-67	3.1463266636202233E-4
68-69	0.0
70-71	0.0011087055862280786
72-73	0.003026466600244215
74-75	0.004539699900366323
76-77	0.002307306219988164
78-79	1.3484257129800956E-4
80-81	9.888455228520701E-4
82-83	8.090554277880574E-4
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3337225.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.13097682106834
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.22990406222868	36.32918039748795
2	8.258391537842046	7.123849879959443
3	2.4460257129255094	3.164984349837819
4	1.1789749556325009	2.0340136593602187
5	0.674063719378548	1.4536513328219636
6	0.4903661652880145	1.2689983025324112
7	0.3298020413655777	0.9957278941175827
8	0.257292294499684	0.8877814392244286
9	0.19845929961647252	0.770376910651518
>10	1.476645227705624	12.728557937261442
>50	0.1826747034362594	5.522533415557504
>100	0.2638553244009139	23.622479289975775
>500	0.012913325622575014	3.4567507948593352
>1k	5.61448940108028E-4	0.38133895964364556
>5k	7.01811175135035E-5	0.25977543670914166
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	8582	0.25715976597322626	No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT	3405	0.10203087894882724	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9965015844002127E-5	0.0	0.0	0.0	0.0
2	2.9965015844002127E-5	0.0	0.0	0.0	0.0
3	2.9965015844002127E-5	0.0	0.0	0.0	0.0
4	5.9930031688004255E-5	0.0	0.0	0.0	0.0
5	2.996501584400213E-4	0.0	0.0	0.0	0.0
6	9.588805070080681E-4	0.0	0.0	2.9965015844002127E-5	0.0
7	0.0014682857763561043	0.0	0.0	2.9965015844002127E-5	0.0
8	0.0027268164418041935	0.0	0.0	2.9965015844002127E-5	0.0
9	0.006712163549056477	0.0	0.0	2.9965015844002127E-5	0.0
10-11	0.01913266261639536	0.0	0.0	2.9965015844002127E-5	2.9965015844002127E-5
12-13	0.03074410625594618	0.0	0.0	2.9965015844002127E-5	2.9965015844002127E-5
14-15	0.04040782386563686	0.0	0.0	2.9965015844002127E-5	8.989504753200639E-5
16-17	0.04857329068312745	0.0	0.0	5.9930031688004255E-5	8.989504753200639E-5
18-19	0.05399695855089183	0.0	0.0	5.9930031688004255E-5	8.989504753200639E-5
20-21	0.060679157084104304	0.0	0.0	5.9930031688004255E-5	8.989504753200639E-5
22-23	0.0677509008232888	0.0	0.0	5.9930031688004255E-5	8.989504753200639E-5
24-25	0.07398362411884125	0.0	0.0	5.9930031688004255E-5	8.989504753200639E-5
26-27	0.08180449325412581	0.0	0.0	5.9930031688004255E-5	8.989504753200639E-5
28-29	0.08781247893084823	0.0	0.0	5.9930031688004255E-5	8.989504753200639E-5
30-31	0.09383544711549266	0.0	0.0	5.9930031688004255E-5	8.989504753200639E-5
32-33	0.09964866018922908	0.0	0.0	5.9930031688004255E-5	1.0487755545400745E-4
34-35	0.10624096367490954	0.0	0.0	5.9930031688004255E-5	1.4982507922001065E-4
36-37	0.112743372113058	0.0	0.0	7.491253961000532E-5	1.4982507922001065E-4
38-39	0.1198151158522425	0.0	0.0	8.989504753200639E-5	1.4982507922001065E-4
40-41	0.12732135232116504	0.0	0.0	8.989504753200639E-5	1.4982507922001065E-4
42-43	0.13647566466150768	0.0	0.0	8.989504753200639E-5	1.4982507922001065E-4
44-45	0.14712822779405044	0.0	0.0	8.989504753200639E-5	1.4982507922001065E-4
46-47	0.16089715257436943	0.0	0.0	8.989504753200639E-5	1.4982507922001065E-4
48-49	0.17689847103506656	0.0	0.0	8.989504753200639E-5	1.7979009506401277E-4
50-51	0.19630081879405792	0.0	0.0	8.989504753200639E-5	1.7979009506401277E-4
52-53	0.21902928331173355	0.0	0.0	8.989504753200639E-5	1.7979009506401277E-4
54-55	0.24452951179497934	0.0	0.0	8.989504753200639E-5	1.7979009506401277E-4
56-57	0.27540846062222357	0.0	0.0	8.989504753200639E-5	1.7979009506401277E-4
58-59	0.31123163706372814	0.0	0.0	8.989504753200639E-5	1.7979009506401277E-4
60-61	0.35321262426117506	0.0	0.0	8.989504753200639E-5	1.7979009506401277E-4
62-63	0.40069219186599647	0.0	0.0	8.989504753200639E-5	1.7979009506401277E-4
64-65	0.45431458771883826	0.0	0.0	8.989504753200639E-5	1.7979009506401277E-4
66-67	0.5162972229921566	0.0	0.0	8.989504753200639E-5	1.7979009506401277E-4
68-69	0.5842129314025875	0.0	0.0	1.0487755545400745E-4	1.7979009506401277E-4
70-71	0.6598895789166148	0.0	0.0	1.1986006337600851E-4	1.7979009506401277E-4
72-73	0.7474773202286331	0.0	0.0	1.4982507922001065E-4	1.7979009506401277E-4
74-75	0.8484444411149983	0.0	0.0	1.4982507922001065E-4	1.7979009506401277E-4
76-77	0.9602738802448142	0.0	0.0	1.4982507922001065E-4	1.7979009506401277E-4
78-79	1.0823064072695128	0.0	0.0	1.4982507922001065E-4	1.7979009506401277E-4
80-81	1.2157256403149324	0.0	0.0	1.4982507922001065E-4	1.7979009506401277E-4
82-83	1.367318655469739	0.0	1.4982507922001064E-5	1.4982507922001065E-4	1.7979009506401277E-4
84-85	1.5326206653731766	0.0	2.9965015844002127E-5	1.4982507922001065E-4	1.7979009506401277E-4
86-87	1.7094891713923994	0.0	2.9965015844002127E-5	1.4982507922001065E-4	1.7979009506401277E-4
88-89	1.9088314392946235	0.0	2.9965015844002127E-5	1.4982507922001065E-4	1.7979009506401277E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGACGG	19510	0.0	84.9528	2
CGACGGC	19565	0.0	84.68588	3
ATATCTC	4450	0.0	83.63797	1
GTCGACG	19820	0.0	83.45979	1
CGGCGCG	20095	0.0	82.33552	6
ATCTCGA	4710	0.0	78.68875	3
TCTCGAG	3520	0.0	77.74746	4
GACGGCG	21640	0.0	76.537895	4
ACGGCGC	21690	0.0	76.32926	5
AGGGCGC	3610	0.0	76.307014	9
CTCGAGG	3640	0.0	75.17422	5
TCGAGGG	3725	0.0	73.70947	6
CGAGGGC	3840	0.0	71.62251	7
CGCGCCG	23425	0.0	70.86165	9
TATCTCG	5310	0.0	69.97948	2
GCGCGCC	23810	0.0	69.83657	8
GGCGCGC	23960	0.0	69.427505	7
GAGGGCG	3980	0.0	69.21418	8
CTCGACG	1800	0.0	53.840054	5
TCTCGAC	2165	0.0	44.988586	4
>>END_MODULE