FastQCFastQC Report
Tue 31 May 2016
SRR2048260_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048260_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3337225
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT132350.3965869846953682No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT88360.2647708799976028No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39000.1168635617916083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC22400.065.354451
CGACGGC45900.058.714153
TCGACGG47200.057.197722
TATCTCG27300.053.2759482
GTCGACG53750.049.166441
GGTCGAC2950.048.33611
ATCTCGA51000.047.7168961
CGGCGCG56800.047.414126
TCTCGAG48700.046.6521152
CTCGAGG55200.041.158663
TCGAGGG56750.039.8669934
GACGGCG68950.039.4996034
CGAGGGC58250.039.3028565
ACGGCGC70150.038.864875
GGGCGCG60450.037.558188
AGGGCGC61650.036.750087
GAGGGCG62500.036.402276
CGCGCCG117400.028.3206319
GGCGCGC120400.027.5755187
GCGCGCC122000.027.3306718
Produced by FastQC (version 0.11.5)