##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048260_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3337225 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.305020188929426 33.0 31.0 34.0 30.0 34.0 2 32.43774573185806 34.0 31.0 34.0 31.0 34.0 3 32.51429915573568 34.0 31.0 34.0 31.0 34.0 4 36.0723010884792 37.0 35.0 37.0 35.0 37.0 5 35.93198121193507 37.0 35.0 37.0 35.0 37.0 6 35.91445767066949 37.0 35.0 37.0 35.0 37.0 7 35.8844219373881 37.0 35.0 37.0 35.0 37.0 8 35.85646427795549 37.0 35.0 37.0 35.0 37.0 9 37.455718748361285 39.0 37.0 39.0 34.0 39.0 10-11 37.45503719407591 39.0 37.0 39.0 34.0 39.0 12-13 37.459839986815396 39.0 37.0 39.0 34.0 39.0 14-15 38.80400782086913 40.0 38.0 41.0 35.0 41.0 16-17 38.81895287252133 40.0 38.0 41.0 35.0 41.0 18-19 38.86421952370607 40.0 38.0 41.0 35.0 41.0 20-21 38.89214571987205 40.0 38.0 41.0 35.0 41.0 22-23 38.86711009895946 40.0 38.0 41.0 35.0 41.0 24-25 38.84896373483957 40.0 38.0 41.0 35.0 41.0 26-27 38.7511409928908 40.0 38.0 41.0 35.0 41.0 28-29 38.64437189581164 40.0 38.0 41.0 34.0 41.0 30-31 38.50327188008001 40.0 38.0 41.0 34.0 41.0 32-33 38.40620485583082 40.0 38.0 41.0 34.0 41.0 34-35 38.35313711242125 40.0 38.0 41.0 34.0 41.0 36-37 38.26181617961031 40.0 38.0 41.0 34.0 41.0 38-39 38.147907318205995 40.0 37.0 41.0 33.0 41.0 40-41 37.98234206563836 40.0 37.0 41.0 33.0 41.0 42-43 37.881896036377526 40.0 37.0 41.0 33.0 41.0 44-45 37.72742068634869 40.0 37.0 41.0 33.0 41.0 46-47 37.570216422326936 40.0 36.0 41.0 32.0 41.0 48-49 37.42355789615624 39.0 36.0 41.0 32.0 41.0 50-51 37.29421315614021 39.0 35.5 41.0 32.0 41.0 52-53 37.21445976822061 39.0 35.0 41.0 32.0 41.0 54-55 36.98739431713474 39.0 35.0 41.0 31.5 41.0 56-57 36.700311336514616 38.5 35.0 40.5 31.0 41.0 58-59 36.41553416386369 38.0 35.0 40.0 31.0 41.0 60-61 36.11000861494206 37.5 35.0 40.0 31.0 41.0 62-63 35.76560855201552 37.0 35.0 40.0 30.5 41.0 64-65 35.450836548329825 36.0 34.0 39.5 30.0 41.0 66-67 35.121974994194275 36.0 34.0 39.0 30.0 41.0 68-69 34.79134041007124 35.0 34.0 39.0 29.5 41.0 70-71 34.47725535437377 35.0 34.0 38.0 29.0 40.0 72-73 34.15582572346785 35.0 34.0 37.0 29.0 40.0 74-75 33.88182666736585 35.0 34.0 37.0 29.0 39.0 76-77 33.694756871352695 35.0 34.0 36.0 29.0 39.0 78-79 33.61132482826301 35.0 34.0 36.0 29.0 39.0 80-81 33.41271011094547 35.0 34.0 36.0 29.0 37.0 82-83 33.21447999460629 35.0 34.0 35.0 29.0 37.0 84-85 33.03430559821408 35.0 34.0 35.0 29.0 36.5 86-87 32.84757965075774 35.0 33.0 35.0 29.0 36.0 88-89 32.71881218677194 35.0 33.0 35.0 29.0 36.0 90-91 32.59251563799265 35.0 33.0 35.0 29.0 36.0 92-93 32.45474863097334 35.0 33.0 35.0 29.0 36.0 94-95 32.34420139487149 35.0 33.0 35.0 29.0 35.0 96-97 32.22625924233458 35.0 33.0 35.0 29.0 35.0 98-99 32.07687824464937 35.0 33.0 35.0 28.5 35.0 100-101 31.623383499764024 34.5 32.5 35.0 26.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4099549112498124 1101 2 0.42024843710082393 1101 3 0.36490720080527694 1101 4 0.24132176485117185 1101 5 0.29335511047587204 1101 6 0.31978691567105244 1101 7 0.341433174080521 1101 8 0.3641338161318046 1101 9 0.4286010435647185 1101 10-11 0.4144724880961874 1101 12-13 0.4156657102936805 1101 14-15 0.5320037380571918 1101 16-17 0.5684804759654796 1101 18-19 0.6241755236283382 1101 20-21 0.567735073772262 1101 22-23 0.5660488658470229 1101 24-25 0.5704832918876477 1101 26-27 0.5699093840718348 1101 28-29 0.5221697703541395 1101 30-31 0.5609616792574528 1101 32-33 0.5628376266692428 1101 34-35 0.5607909360168648 1101 36-37 0.568898584529407 1101 38-39 0.5050075977433792 1101 40-41 0.5824302076585823 1101 42-43 0.4808352681300647 1101 44-45 0.5462070173941598 1101 46-47 0.5301592413800975 1101 48-49 0.5499993390489877 1101 50-51 0.6063646277654229 1101 52-53 0.5361900737900598 1101 54-55 0.5188166175245357 1101 56-57 0.5439766411330424 1101 58-59 0.4379990634707127 1101 60-61 0.37444149189384035 1101 62-63 0.4397261076258303 1101 64-65 0.3441380711665616 1101 66-67 0.3717691758012691 1101 68-69 0.38451177719871765 1101 70-71 0.31614720393391593 1101 72-73 0.331707870906925 1101 74-75 0.3911214358342363 1101 76-77 0.37506384808127535 1101 78-79 0.32697611160786266 1101 80-81 0.291692453261561 1101 82-83 0.3912028623161916 1101 84-85 0.2839938651089682 1101 86-87 0.28362190178282276 1101 88-89 0.29634191704948165 1101 90-91 0.3508000183878366 1101 92-93 0.39547245252002483 1101 94-95 0.3986789060242302 1101 96-97 0.4657497877159571 1101 98-99 0.4714942129600672 1101 100-101 0.46654262084122067 1102 1 0.3426902583895952 1102 2 0.3236940027693862 1102 3 0.34407661557025904 1102 4 0.21435467607660286 1102 5 0.2428791398475525 1102 6 0.278375272528109 1102 7 0.2857902289985148 1102 8 0.2938676902908881 1102 9 0.30921980538153804 1102 10-11 0.37386768661880865 1102 12-13 0.3637684232163352 1102 14-15 0.5085275585733982 1102 16-17 0.5096387444436914 1102 18-19 0.4421689721619515 1102 20-21 0.5240133791849217 1102 22-23 0.541394026995512 1102 24-25 0.47070946037904804 1102 26-27 0.48955856184081625 1102 28-29 0.5346763657900908 1102 30-31 0.5128166456207239 1102 32-33 0.5699716898974927 1102 34-35 0.5627390629130531 1102 36-37 0.48767304260442046 1102 38-39 0.49809777933771215 1102 40-41 0.5377689382570097 1102 42-43 0.4848876139067926 1102 44-45 0.5294389458996349 1102 46-47 0.5268271721719913 1102 48-49 0.5425728781599233 1102 50-51 0.5259321870144191 1102 52-53 0.5540117661789239 1102 54-55 0.5592801667484721 1102 56-57 0.521750571573179 1102 58-59 0.4621694125891267 1102 60-61 0.48463556259691387 1102 62-63 0.47046688500788036 1102 64-65 0.5112400366064804 1102 66-67 0.5201052022709547 1102 68-69 0.5703398341832866 1102 70-71 0.5022385287371591 1102 72-73 0.47570300127670606 1102 74-75 0.4657625341941767 1102 76-77 0.45423601094457666 1102 78-79 0.4637756213471249 1102 80-81 0.48573622497153934 1102 82-83 0.47017029185495574 1102 84-85 0.4352931659927535 1102 86-87 0.4351416001515531 1102 88-89 0.389315854085055 1102 90-91 0.3482091112955388 1102 92-93 0.38326677384966246 1102 94-95 0.35094196498742036 1102 96-97 0.3931304652870793 1102 98-99 0.41983958296403046 1102 100-101 0.456219939136961 1103 1 0.26199358835587816 1103 2 0.3084180131073282 1103 3 0.2836489113259617 1103 4 0.2110544692536891 1103 5 0.24037838519456756 1103 6 0.20980638257731243 1103 7 0.17211975688974235 1103 8 0.19193993858078784 1103 9 0.3481465661762826 1103 10-11 0.29522271271433453 1103 12-13 0.30338658480700076 1103 14-15 0.4185777229970782 1103 16-17 0.3773470100004701 1103 18-19 0.39901722131369866 1103 20-21 0.36516212635430634 1103 22-23 0.4299955136885458 1103 24-25 0.42217247384974144 1103 26-27 0.45313548897920697 1103 28-29 0.41421668345900287 1103 30-31 0.468147855614788 1103 32-33 0.4557161617310683 1103 34-35 0.43012173437394097 1103 36-37 0.4381414074447392 1103 38-39 0.4657999650047415 1103 40-41 0.5192036749071178 1103 42-43 0.4339130715784876 1103 44-45 0.461550843603888 1103 46-47 0.5210635433524615 1103 48-49 0.4341220447350338 1103 50-51 0.4489597161152261 1103 52-53 0.4392087812730523 1103 54-55 0.4753107560604519 1103 56-57 0.4652057302529684 1103 58-59 0.3472648295255638 1103 60-61 0.4228876156263581 1103 62-63 0.3809087756615952 1103 64-65 0.31263377133342374 1103 66-67 0.3518602531126973 1103 68-69 0.29285313260880486 1103 70-71 0.3092278953299754 1103 72-73 0.2990288716342562 1103 74-75 0.24655024257705804 1103 76-77 0.2952596937151313 1103 78-79 0.26358218485477636 1103 80-81 0.22761263894589945 1103 82-83 0.19898400936621385 1103 84-85 0.27664564280117787 1103 86-87 0.2650593509763297 1103 88-89 0.24508913606287308 1103 90-91 0.26540554613352185 1103 92-93 0.28658479368290557 1103 94-95 0.30946298380628434 1103 96-97 0.21754058265028675 1103 98-99 0.23464640780026969 1103 100-101 0.24332753291925968 1104 1 0.25437811480129113 1104 2 0.2660666742280853 1104 3 0.21971431832506738 1104 4 0.1559555642703856 1104 5 0.1921567448108661 1104 6 0.19649392486228834 1104 7 0.19271927221951302 1104 8 0.19865227750220527 1104 9 0.29085927668655387 1104 10-11 0.22547033973158648 1104 12-13 0.2995013907456894 1104 14-15 0.3953338207752992 1104 16-17 0.3512214538961942 1104 18-19 0.3670344112731101 1104 20-21 0.360841228169285 1104 22-23 0.4003449426540939 1104 24-25 0.3921156431331241 1104 26-27 0.39515075268325717 1104 28-29 0.40875648781687346 1104 30-31 0.43407287078051127 1104 32-33 0.44289485570372733 1104 34-35 0.31215033420167515 1104 36-37 0.3983609547703111 1104 38-39 0.38749190224067576 1104 40-41 0.3973228994259159 1104 42-43 0.3868800848970224 1104 44-45 0.41533263982818625 1104 46-47 0.444748188378 1104 48-49 0.35307077217203187 1104 50-51 0.374360898471501 1104 52-53 0.42439813516410396 1104 54-55 0.3688525373917457 1104 56-57 0.3652463230961871 1104 58-59 0.3267252888426597 1104 60-61 0.36999518507824547 1104 62-63 0.32952459982587357 1104 64-65 0.17168219912169747 1104 66-67 0.19935394592929612 1104 68-69 0.18794904677296387 1104 70-71 0.1850067652184464 1104 72-73 0.22368448392516171 1104 74-75 0.19663659576299608 1104 76-77 0.19980368507260238 1104 78-79 0.1991936873847493 1104 80-81 0.1588807735014015 1104 82-83 0.18931392108368073 1104 84-85 0.2206944868602534 1104 86-87 0.20802294653131526 1104 88-89 0.19062658066959415 1104 90-91 0.2238366012743498 1104 92-93 0.258796693992565 1104 94-95 0.23121382832807313 1104 96-97 0.23417772267634263 1104 98-99 0.27207266695684496 1104 100-101 0.2698357747496978 1105 1 0.16404034855673189 1105 2 0.1991887268632624 1105 3 0.17248683221041006 1105 4 0.08606848904420161 1105 5 0.1373015257310115 1105 6 0.16347233167190467 1105 7 0.11243162939105389 1105 8 0.13190706637149674 1105 9 0.19592420325365367 1105 10-11 0.20359840692009357 1105 12-13 0.21574028150918423 1105 14-15 0.29449508246967326 1105 16-17 0.2514476113654993 1105 18-19 0.18213067436872166 1105 20-21 0.23477859140302826 1105 22-23 0.2318553002776227 1105 24-25 0.18477633013203132 1105 26-27 0.25918777439888174 1105 28-29 0.17938054056826047 1105 30-31 0.2272488317023189 1105 32-33 0.2527598595445042 1105 34-35 0.20984614534891932 1105 36-37 0.30275791125786355 1105 38-39 0.2558172280832096 1105 40-41 0.2912555778514019 1105 42-43 0.27589891998901805 1105 44-45 0.23713263260668782 1105 46-47 0.2135238679913698 1105 48-49 0.25322720737752746 1105 50-51 0.261432807162457 1105 52-53 0.28971556116621144 1105 54-55 0.23080793530444055 1105 56-57 0.19649208802768925 1105 58-59 0.19944907412374846 1105 60-61 0.26317790927958384 1105 62-63 0.10387732739785349 1105 64-65 0.18103484329823516 1105 66-67 0.21191845139517795 1105 68-69 0.15094274555921317 1105 70-71 0.1343772028686132 1105 72-73 0.0913708356453995 1105 74-75 0.12131345671417648 1105 76-77 0.14538050942142888 1105 78-79 0.16562597311846616 1105 80-81 0.1691546350022648 1105 82-83 0.11536726521381979 1105 84-85 0.1437893269178545 1105 86-87 0.18170568786996455 1105 88-89 0.14392168161750618 1105 90-91 0.14002714100421798 1105 92-93 0.11750624926019526 1105 94-95 0.012527180233234958 1105 96-97 0.04830478398739757 1105 98-99 0.11041439512689522 1105 100-101 0.11905740192144165 1106 1 0.1547316506059957 1106 2 0.1259668023285343 1106 3 0.09689503643797082 1106 4 0.077951715897008 1106 5 0.10237083514580547 1106 6 0.05531057684891749 1106 7 0.06474388465758807 1106 8 0.11605971733340681 1106 9 0.19766164288233057 1106 10-11 0.15232073019676307 1106 12-13 0.16807484840374798 1106 14-15 0.19809092838503517 1106 16-17 0.21048722570587586 1106 18-19 0.15073167256765885 1106 20-21 0.19245432907563043 1106 22-23 0.2459190631471273 1106 24-25 0.15366549779181327 1106 26-27 0.11753492182587877 1106 28-29 0.1705353689300182 1106 30-31 0.19371535081953084 1106 32-33 0.27102483924898735 1106 34-35 0.2480433837249194 1106 36-37 0.19115084680503713 1106 38-39 0.23873404782496976 1106 40-41 0.2574956653058109 1106 42-43 0.26931286789271525 1106 44-45 0.2308904842388415 1106 46-47 0.20479894617167105 1106 48-49 0.22473454154120276 1106 50-51 0.2579704725955665 1106 52-53 0.0897542704967762 1106 54-55 0.1849963386341642 1106 56-57 0.14776205872687598 1106 58-59 0.1072067107040624 1106 60-61 0.04459447364728675 1106 62-63 0.06834834468264717 1106 64-65 0.029870158393983104 1106 66-67 0.06442586620328683 1106 68-69 0.06573006825345828 1106 70-71 0.036063388476271996 1106 72-73 0.01695393973944448 1106 74-75 0.003674098643912771 1106 76-77 0.0583107699029668 1106 78-79 0.10727335979062502 1106 80-81 0.0885968443014633 1106 82-83 0.1163487976373645 1106 84-85 0.1231616443701995 1106 86-87 0.1162250022130209 1106 88-89 0.1595326528453569 1106 90-91 0.1735199412479389 1106 92-93 0.21840803329439495 1106 94-95 0.21106375248617582 1106 96-97 0.2577578354833534 1106 98-99 0.2943062957371012 1106 100-101 0.195715406778028 1107 1 -0.005278922775964645 1107 2 0.08892985821513122 1107 3 0.07411674592086115 1107 4 0.10370990802790203 1107 5 0.08361025056322546 1107 6 0.09137261930373342 1107 7 0.12106058432873823 1107 8 0.09144685544148956 1107 9 0.17080435665729254 1107 10-11 -4.284387174919857E-4 1107 12-13 0.10087930356095853 1107 14-15 0.09909719176977205 1107 16-17 0.05998068902609788 1107 18-19 0.054805650399401884 1107 20-21 0.03669613694473384 1107 22-23 0.11919949970235422 1107 24-25 0.11497767241390733 1107 26-27 -0.022499665677820246 1107 28-29 0.10315810528541647 1107 30-31 0.12398900428141957 1107 32-33 0.10788929386856694 1107 34-35 0.0890407762214096 1107 36-37 0.027973635131303354 1107 38-39 0.13271080430563842 1107 40-41 0.11599191533942133 1107 42-43 0.10245692574165588 1107 44-45 0.16762160671982684 1107 46-47 0.12407036847076114 1107 48-49 0.08285328599689734 1107 50-51 0.16411737092084167 1107 52-53 0.2032342572352448 1107 54-55 0.06772068698229816 1107 56-57 0.17827540538147701 1107 58-59 0.10817390633851431 1107 60-61 0.16816352768803 1107 62-63 -0.08548029484567365 1107 64-65 0.025234268034573404 1107 66-67 0.005590551107545139 1107 68-69 0.12045377144607983 1107 70-71 0.18509628244675724 1107 72-73 0.0062318135936223484 1107 74-75 0.02505267052050897 1107 76-77 0.02284934930637661 1107 78-79 -0.06237019179044978 1107 80-81 -0.04465169289802162 1107 82-83 -0.1020823992157105 1107 84-85 -0.08353569705146668 1107 86-87 -0.17572480110415256 1107 88-89 0.0323205310901713 1107 90-91 -0.05086451767267164 1107 92-93 -0.0420335398097933 1107 94-95 -0.0014709022212215928 1107 96-97 0.056933738925074806 1107 98-99 -0.016239487902829808 1107 100-101 -0.04853607842371943 1108 1 -0.04228827533299295 1108 2 0.0316448132613516 1108 3 -0.007925982782623464 1108 4 -0.0020183070266384107 1108 5 -0.0014891542740542718 1108 6 -0.022134318231316286 1108 7 -0.029847898096775793 1108 8 -0.025495563922099507 1108 9 -0.21034399986145758 1108 10-11 0.05468362915638636 1108 12-13 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2214 90-91 -0.19073957800251407 2214 92-93 -0.22971532611924772 2214 94-95 -0.2858008865712378 2214 96-97 -0.26625321135793456 2214 98-99 -0.3541078901606909 2214 100-101 -0.5067956391574846 2215 1 -0.6262890477258907 2215 2 -0.9629639585968093 2215 3 -0.580961004116265 2215 4 -0.37166159519231456 2215 5 -0.31205619463529644 2215 6 -0.2656584722197195 2215 7 -0.3484759723504709 2215 8 -0.3924400444693106 2215 9 -0.4429155854578113 2215 10-11 -0.47772931882221314 2215 12-13 -0.3949008885823133 2215 14-15 -0.5351314301542871 2215 16-17 -0.5222051392896105 2215 18-19 -0.5109540284220984 2215 20-21 -0.49976074007515336 2215 22-23 -0.5411899760257484 2215 24-25 -0.5708982517092593 2215 26-27 -0.5062470991538817 2215 28-29 -0.4906645751534029 2215 30-31 -0.4819267425942684 2215 32-33 -0.5622111857442533 2215 34-35 -0.6505963166799233 2215 36-37 -0.5285663330127619 2215 38-39 -0.47730481419903725 2215 40-41 -0.5833054500828325 2215 42-43 -0.6605148207475793 2215 44-45 -0.5884062359961533 2215 46-47 -0.6033627226439862 2215 48-49 -0.7091294413748201 2215 50-51 -0.5903729067156718 2215 52-53 -0.49452092001506287 2215 54-55 -0.5693534011881951 2215 56-57 -0.41897430746787023 2215 58-59 -0.541863804887285 2215 60-61 -0.4652096711692977 2215 62-63 -0.4392780332288382 2215 64-65 -0.36706379198373185 2215 66-67 -0.35622585626653347 2215 68-69 -0.41509635674567136 2215 70-71 -0.38788704441738986 2215 72-73 -0.320085829089642 2215 74-75 -0.346631529617504 2215 76-77 -0.23210599256376696 2215 78-79 -0.25999673385125277 2215 80-81 -0.2912062152920001 2215 82-83 -0.32737535703819987 2215 84-85 -0.3155319125313554 2215 86-87 -0.27700307673971736 2215 88-89 -0.25213699052088856 2215 90-91 -0.2939250388593635 2215 92-93 -0.32942966882841773 2215 94-95 -0.32914129950336957 2215 96-97 -0.3338712131806254 2215 98-99 -0.4089717034793239 2215 100-101 -0.42972363954996595 2216 1 -0.5392036084591254 2216 2 -0.5586424127974574 2216 3 -0.48977881448740845 2216 4 -0.34304827283847317 2216 5 -0.41199780744233294 2216 6 -0.3948395040678605 2216 7 -0.42066856563773314 2216 8 -0.4717763313743646 2216 9 -0.5949616003223497 2216 10-11 -0.73737516538867 2216 12-13 -0.7396331602924562 2216 14-15 -0.995098169064299 2216 16-17 -1.345169026288886 2216 18-19 -1.3539512500488655 2216 20-21 -1.6345247902644147 2216 22-23 -1.4377601158948607 2216 24-25 -1.4716028793291969 2216 26-27 -1.3561973506402651 2216 28-29 -1.281838102381144 2216 30-31 -1.4434269162771898 2216 32-33 -1.189163240638969 2216 34-35 -0.7442432381590294 2216 36-37 -1.064490310736744 2216 38-39 -1.4468781599077474 2216 40-41 -1.4232588320526531 2216 42-43 -0.7480259274798229 2216 44-45 -1.245157247275145 2216 46-47 -0.9110951748768272 2216 48-49 -0.8488690150423466 2216 50-51 -0.9608547884596206 2216 52-53 -1.0593932308455791 2216 54-55 -1.1472163880173127 2216 56-57 -1.224856303187046 2216 58-59 -0.9151652962198611 2216 60-61 -0.9545789360845021 2216 62-63 -0.8092344776796736 2216 64-65 -0.9880100472210103 2216 66-67 -0.709356308073815 2216 68-69 -0.710346312682475 2216 70-71 -0.6911469538524031 2216 72-73 -0.6480256151619344 2216 74-75 -0.6216008118970464 2216 76-77 -0.6378277160708379 2216 78-79 -0.5160166677323446 2216 80-81 -0.6038655415983669 2216 82-83 -0.7411406794262021 2216 84-85 -0.6436496712127564 2216 86-87 -0.7033980494948651 2216 88-89 -0.7211378112152005 2216 90-91 -0.7656142679641817 2216 92-93 -0.6914582568870813 2216 94-95 -0.7525970653928695 2216 96-97 -0.7646336215068423 2216 98-99 -1.1326658771507034 2216 100-101 -0.933480456891278 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 21.0 13 136.0 14 516.0 15 1166.0 16 2031.0 17 3486.0 18 6444.0 19 12186.0 20 13406.0 21 12068.0 22 11926.0 23 12397.0 24 14071.0 25 16950.0 26 21814.0 27 28519.0 28 37401.0 29 48016.0 30 61898.0 31 80363.0 32 103648.0 33 136238.0 34 185531.0 35 264575.0 36 475900.0 37 848410.0 38 750052.0 39 187757.0 40 297.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.05383215096375 38.75036894425758 13.926001393373236 21.269797511405432 2 23.92170141359962 28.564121388279183 29.820224887443906 17.693952310677286 3 21.54334814104533 27.59586183131194 25.325712230970343 25.535077796672383 4 23.458077675281398 27.056545945304265 26.153204174305934 23.332172205108403 5 23.714253608911594 27.6373034482242 25.40080456067541 23.247638382188796 6 23.573987369745822 26.447692319217314 26.1075294593562 23.870790851680663 7 24.1393972537063 27.472795511240626 24.92513989916772 23.462667335885353 8 24.152641790709346 26.052723445377524 26.32489568428859 23.46973907962454 9 22.891863749072957 25.64442013948715 27.17437391845021 24.289342192989686 10-11 24.044827663702627 26.533826757260897 25.659043067219024 23.762302511817452 12-13 23.596236394009992 26.623512049681995 25.915903183033805 23.864348373274204 14-15 23.508259107492 26.46222535190165 26.076950160687396 23.952565379918944 16-17 23.753477814651394 26.65870596079078 25.932249099176712 23.655567125381115 18-19 22.908689105469364 26.504371146686246 27.02400946894501 23.562930278899387 20-21 22.45686161406558 26.91285424267168 26.758639288630526 23.87164485463222 22-23 22.34669858141099 26.942478697492483 27.469383615036985 23.24143910605954 24-25 22.22822854317584 26.29466098330199 28.681089827626245 22.79602064589592 26-27 22.3471165018215 26.05475061971445 28.958512655359357 22.639620223104693 28-29 22.366677164429426 26.226447829764844 28.890792003761216 22.516083002044518 30-31 22.302128955083628 26.24567604691804 28.984898826090188 22.46729617190815 32-33 22.26172943088944 26.172853193896128 28.964993370240244 22.600424004974194 34-35 22.57344050820667 26.40680505509817 28.81927349819086 22.200480938504295 36-37 22.358666257144783 26.424469431938213 28.639198735476327 22.577665575440673 38-39 22.492028930028532 26.424862936251365 28.60802442269017 22.475083711029928 40-41 22.52032603155378 26.517601610680497 28.196842104947738 22.765230252817986 42-43 22.64758413693299 26.71151632487306 27.9565033627505 22.68439617544345 44-45 22.645284185809857 26.52922284369795 28.035102891090723 22.790390079401465 46-47 22.744762783165996 27.01319451124154 27.671631576092803 22.570411129499664 48-49 22.68560296138303 27.06043600003536 27.513791775171253 22.74016926341036 50-51 22.746710511530402 26.694710958608976 27.657712809550222 22.9008657203104 52-53 22.785305504060837 27.00779085584647 27.52945861650018 22.67744502359251 54-55 22.684816653519388 26.947995957679993 27.509845476399697 22.85734191240092 56-57 22.7576525089352 27.07143440390924 27.335501303853725 22.835411783301833 58-59 22.999529695059913 27.15539825119678 27.15505365057167 22.690018403171646 60-61 22.761012114607077 27.287201798148214 27.117074706993353 22.834711380251356 62-63 22.75982094658827 26.95679168552595 27.370789398889762 22.912597968996018 64-65 22.87945911874867 27.248184529610793 27.093085398462584 22.779270953177953 66-67 22.803242998177677 26.882251262690016 27.372869128665606 22.941636610466702 68-69 22.924281569672356 27.11786446506449 26.86873390494923 23.089120060313927 70-71 23.023866336290993 27.081880648241903 27.02782353288419 22.866429482582912 72-73 22.83022783167739 27.06265464768981 26.855716093248205 23.2514014273846 74-75 22.959173812484295 27.036848311994888 26.878946880304298 23.125030995216513 76-77 22.928635586243267 27.222696255321637 26.940783485569124 22.907884672865972 78-79 23.03031247382742 27.212981090418424 26.677386156486072 23.07932027926808 80-81 22.863478747605797 27.394819766368695 26.64561923323097 23.096082252794538 82-83 22.913056122446687 27.48748673459771 26.502756761478363 23.096700381477238 84-85 22.987362254567792 27.407636584287843 26.67166583014331 22.933335331001057 86-87 23.03074006558444 27.212688268349943 26.765265567240082 22.991306098825536 88-89 23.04997059671807 27.40823366806879 26.585855710417 22.955940024796142 90-91 23.04047399981392 27.330561262657067 26.728727369051423 22.90023736847759 92-93 22.96400530502389 27.209282952786367 26.723593392572365 23.103118349617375 94-95 23.140198817880126 27.56522260261145 26.353901819625587 22.940676759882837 96-97 23.241319886378193 27.38403005023805 26.408455082933763 22.966194980449995 98-99 23.124088033061017 27.357637269424718 26.4904792861491 23.027795411365165 100-101 23.230805468765453 27.56731219324202 26.15758152592485 23.04430081206768 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1619.0 1 1183.0 2 519.0 3 228.0 4 141.5 5 111.5 6 92.0 7 79.5 8 79.0 9 78.0 10 90.0 11 100.5 12 111.0 13 203.5 14 281.0 15 475.0 16 2083.0 17 2338.0 18 1291.0 19 1557.5 20 2042.5 21 2804.0 22 3747.0 23 4891.0 24 6147.0 25 7743.0 26 10192.0 27 13331.5 28 16881.0 29 21713.0 30 28950.5 31 37125.0 32 44543.5 33 53041.0 34 62376.0 35 71866.0 36 79266.0 37 86100.0 38 97091.5 39 111505.0 40 115550.5 41 109692.0 42 106067.5 43 110605.5 44 121584.5 45 131419.5 46 140195.5 47 145415.5 48 143451.5 49 137775.5 50 134064.5 51 135136.0 52 142958.5 53 143187.5 54 124105.0 55 109188.0 56 100555.5 57 80403.5 58 64785.5 59 59656.0 60 54048.0 61 44309.5 62 32164.5 63 23244.0 64 16470.5 65 9955.0 66 6133.0 67 4189.0 68 3123.0 69 2432.5 70 2107.0 71 1962.0 72 1954.0 73 1657.5 74 1207.5 75 1027.0 76 827.0 77 586.5 78 395.5 79 233.0 80 86.5 81 47.0 82 39.5 83 14.0 84 3.5 85 2.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.04162140700731895 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.4982507922001064E-5 24-25 0.0 26-27 7.491253961000532E-5 28-29 1.7979009506401277E-4 30-31 2.9965015844002127E-5 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.4982507922001064E-5 40-41 5.9930031688004255E-5 42-43 4.494752376600319E-5 44-45 2.097551109080149E-4 46-47 1.1986006337600851E-4 48-49 1.4982507922001064E-5 50-51 1.1986006337600851E-4 52-53 0.0013034781892140926 54-55 9.738630149300691E-4 56-57 7.491253961000532E-5 58-59 8.540029515540607E-4 60-61 4.195102218160298E-4 62-63 2.547026346740181E-4 64-65 1.3484257129800956E-4 66-67 1.3484257129800956E-4 68-69 5.693353010360404E-4 70-71 4.195102218160298E-4 72-73 7.491253961000532E-5 74-75 4.944227614260351E-4 76-77 6.742128564900479E-4 78-79 4.494752376600319E-5 80-81 2.9965015844002127E-5 82-83 0.0019926735536261414 84-85 0.0 86-87 1.4982507922001064E-5 88-89 3.745626980500266E-4 90-91 2.547026346740181E-4 92-93 3.296151742840234E-4 94-95 0.0 96-97 4.045277138940287E-4 98-99 4.494752376600319E-5 100-101 0.009319119927484663 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3337225.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.48314936561592 #Duplication Level Percentage of deduplicated Percentage of total 1 83.72352240272187 33.8939186284488 2 8.410948662380434 6.810033820113491 3 2.51170593635232 3.0504529975156554 4 1.1876349826275492 1.9231681757416699 5 0.7356784697419537 1.4891290687815633 6 0.5020112644028965 1.2193798200026498 7 0.3501144278776356 0.9921614275179239 8 0.25340216497519874 0.8206814155409135 9 0.22084522418599678 0.8046459177664177 >10 1.5903109255556953 12.964393521899368 >50 0.2006837146027594 5.697800602160664 >100 0.29708931919387804 25.293337924499138 >500 0.015160699527463399 3.84901268791913 >1k 7.431715454625316E-4 0.5278575484971831 >5k 7.431715454625316E-5 0.26583923046572305 >10k+ 7.431715454625316E-5 0.39818721312967903 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13235 0.3965869846953682 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 8836 0.2647708799976028 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3900 0.1168635617916083 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.9965015844002127E-5 0.0 0.0 0.0 0.0 2 2.9965015844002127E-5 0.0 0.0 0.0 0.0 3 2.9965015844002127E-5 0.0 0.0 0.0 0.0 4 5.9930031688004255E-5 0.0 0.0 0.0 0.0 5 2.6968514259601917E-4 0.0 0.0 0.0 0.0 6 9.289154911640659E-4 0.0 0.0 0.0 0.0 7 0.0014383207605121022 0.0 0.0 0.0 0.0 8 0.0025170613308961786 0.0 0.0 2.9965015844002127E-5 0.0 9 0.006322618343084449 0.0 0.0 2.9965015844002127E-5 0.0 10-11 0.017949044490557276 0.0 0.0 2.9965015844002127E-5 0.0 12-13 0.02806223733790799 0.0 0.0 2.9965015844002127E-5 0.0 14-15 0.03591307148903655 0.0 0.0 2.9965015844002127E-5 0.0 16-17 0.04258028751432702 0.0 0.0 2.9965015844002127E-5 0.0 18-19 0.047134969922615344 0.0 0.0 2.9965015844002127E-5 1.4982507922001064E-5 20-21 0.05267849785375574 0.0 0.0 2.9965015844002127E-5 2.9965015844002127E-5 22-23 0.05864153600671217 0.0 0.0 2.9965015844002127E-5 2.9965015844002127E-5 24-25 0.06424499396954056 0.0 0.0 2.9965015844002127E-5 2.9965015844002127E-5 26-27 0.07086726247106503 0.0 0.0 2.9965015844002127E-5 2.9965015844002127E-5 28-29 0.07639580789428342 0.0 0.0 5.9930031688004255E-5 2.9965015844002127E-5 30-31 0.08207417839672182 0.0 0.0 5.9930031688004255E-5 2.9965015844002127E-5 32-33 0.08776753140708224 0.0 0.0 5.9930031688004255E-5 2.9965015844002127E-5 34-35 0.09412011476601068 0.0 0.0 5.9930031688004255E-5 2.9965015844002127E-5 36-37 0.10044273310909513 0.0 0.0 5.9930031688004255E-5 2.9965015844002127E-5 38-39 0.10745454681659163 0.0 0.0 5.9930031688004255E-5 2.9965015844002127E-5 40-41 0.11521548592018818 0.0 0.0 5.9930031688004255E-5 2.9965015844002127E-5 42-43 0.12493913356156688 0.0 0.0 5.9930031688004255E-5 2.9965015844002127E-5 44-45 0.13632583958228767 0.0 0.0 5.9930031688004255E-5 7.491253961000532E-5 46-47 0.1504693270606567 0.0 0.0 5.9930031688004255E-5 8.989504753200639E-5 48-49 0.16671036564810582 0.0 0.0 5.9930031688004255E-5 8.989504753200639E-5 50-51 0.1860527833754092 0.0 0.0 5.9930031688004255E-5 8.989504753200639E-5 52-53 0.20897602049607084 0.0 0.0 5.9930031688004255E-5 8.989504753200639E-5 54-55 0.23480586415360066 0.0 0.0 5.9930031688004255E-5 8.989504753200639E-5 56-57 0.2660893406947389 0.0 0.0 5.9930031688004255E-5 8.989504753200639E-5 58-59 0.30198742967585346 0.0 0.0 5.9930031688004255E-5 8.989504753200639E-5 60-61 0.3441781719842084 0.0 0.0 8.989504753200639E-5 8.989504753200639E-5 62-63 0.3913580894305898 0.0 0.0 8.989504753200639E-5 8.989504753200639E-5 64-65 0.4448006951883676 0.0 0.0 8.989504753200639E-5 1.1986006337600851E-4 66-67 0.50666347039831 0.0 0.0 8.989504753200639E-5 1.1986006337600851E-4 68-69 0.5746091438245848 0.0 0.0 8.989504753200639E-5 1.3484257129800959E-4 70-71 0.6503906688940662 0.0 0.0 8.989504753200639E-5 1.4982507922001065E-4 72-73 0.7381881653169924 0.0 0.0 8.989504753200639E-5 1.4982507922001065E-4 74-75 0.8384810733468675 0.0 0.0 8.989504753200639E-5 1.4982507922001065E-4 76-77 0.9500707923499314 0.0 0.0 8.989504753200639E-5 1.4982507922001065E-4 78-79 1.070949666264636 0.0 0.0 8.989504753200639E-5 1.4982507922001065E-4 80-81 1.2037096689614875 0.0 0.0 1.3484257129800959E-4 1.4982507922001065E-4 82-83 1.3545835237360382 0.0 0.0 1.4982507922001065E-4 1.4982507922001065E-4 84-85 1.5189865831641558 0.0 0.0 1.4982507922001065E-4 1.4982507922001065E-4 86-87 1.6948063136288383 0.0 0.0 1.4982507922001065E-4 1.648075871420117E-4 88-89 1.8928900508656143 0.0 0.0 1.4982507922001065E-4 1.7979009506401277E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATATCTC 2240 0.0 65.35445 1 CGACGGC 4590 0.0 58.71415 3 TCGACGG 4720 0.0 57.19772 2 TATCTCG 2730 0.0 53.275948 2 GTCGACG 5375 0.0 49.16644 1 GGTCGAC 295 0.0 48.3361 1 ATCTCGA 5100 0.0 47.716896 1 CGGCGCG 5680 0.0 47.41412 6 TCTCGAG 4870 0.0 46.652115 2 CTCGAGG 5520 0.0 41.15866 3 TCGAGGG 5675 0.0 39.866993 4 GACGGCG 6895 0.0 39.499603 4 CGAGGGC 5825 0.0 39.302856 5 ACGGCGC 7015 0.0 38.86487 5 GGGCGCG 6045 0.0 37.55818 8 AGGGCGC 6165 0.0 36.75008 7 GAGGGCG 6250 0.0 36.40227 6 CGCGCCG 11740 0.0 28.320631 9 GGCGCGC 12040 0.0 27.575518 7 GCGCGCC 12200 0.0 27.330671 8 >>END_MODULE