FastQCFastQC Report
Tue 31 May 2016
SRR2048259_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048259_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4439606
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT131380.2959271611039358No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT50270.11323076867631948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG260700.085.919031
ATATCTC81850.085.798381
TCGACGG274100.081.863532
ATCTCGA86050.081.526913
CGACGGC277300.080.9359743
TCTCGAG60750.079.564734
CGGCGCG284700.078.7431266
TATCTCG89400.078.52512
CTCGAGG63250.076.4786455
TCGAGGG63800.075.801416
AGGGCGC63900.075.214689
GACGGCG299100.075.0812154
ACGGCGC299350.075.03425
CGAGGGC65450.073.958867
GAGGGCG67800.070.963018
CTCGACG33250.065.309675
CGCGCCG344900.065.25549
GCGCGCC351350.064.115868
GGCGCGC351650.063.9909367
TCTCGAC38250.056.908874
Produced by FastQC (version 0.11.5)