FastQCFastQC Report
Tue 31 May 2016
SRR2048259_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048259_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4439606
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT194660.4384623320177511No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT171060.3853044617022321No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT51390.11575351506417461No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48620.10951422265849718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC40650.070.950621
TCGACGG73500.059.215612
TATCTCG48900.058.8832552
CGACGGC75100.057.890763
GTCGACG74050.057.3641471
GGTCGAC4350.052.4300271
CGGCGCG90300.048.182476
GACGGCG99300.044.0216834
ATCTCGA87150.043.39841
ACGGCGC100700.043.3955
TCTCGAG81500.040.5186772
CTCGAGG91200.036.1049233
TCGAGGG92300.035.726124
CGAGGGC95050.034.7307365
GGGCGCG99450.033.098618
AGGGCGC100000.033.0590637
GAGGGCG101100.032.5584266
CGCGCCG188400.027.5310659
GCGCGCC195000.026.696688
GGCGCGC195650.026.6322657
Produced by FastQC (version 0.11.5)