##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048259_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4439606 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.31540051076605 33.0 31.0 34.0 31.0 34.0 2 32.443633061132005 34.0 31.0 34.0 31.0 34.0 3 32.52134941704286 34.0 31.0 34.0 31.0 34.0 4 36.07947912494937 37.0 35.0 37.0 35.0 37.0 5 35.93756427935272 37.0 35.0 37.0 35.0 37.0 6 35.921963345395966 37.0 35.0 37.0 35.0 37.0 7 35.89220845273206 37.0 35.0 37.0 35.0 37.0 8 35.86135368769211 37.0 35.0 37.0 35.0 37.0 9 37.45635310881191 39.0 37.0 39.0 34.0 39.0 10-11 37.45591309228792 39.0 37.0 39.0 34.0 39.0 12-13 37.46031438375387 39.0 37.0 39.0 34.0 39.0 14-15 38.805751231077714 40.0 38.0 41.0 35.0 41.0 16-17 38.82151760764356 40.0 38.0 41.0 35.0 41.0 18-19 38.8679242031838 40.0 38.0 41.0 35.0 41.0 20-21 38.89580854697466 40.0 38.0 41.0 35.0 41.0 22-23 38.87887067005495 40.0 38.0 41.0 35.0 41.0 24-25 38.86289864460946 40.0 38.0 41.0 35.0 41.0 26-27 38.76767364040863 40.0 38.0 41.0 35.0 41.0 28-29 38.65987353382259 40.0 38.0 41.0 34.5 41.0 30-31 38.507595719079575 40.0 38.0 41.0 34.0 41.0 32-33 38.39770680100892 40.0 38.0 41.0 34.0 41.0 34-35 38.34220750670217 40.0 38.0 41.0 34.0 41.0 36-37 38.25073745282802 40.0 37.5 41.0 34.0 41.0 38-39 38.139713298882825 40.0 37.0 41.0 33.5 41.0 40-41 37.964285118994795 40.0 37.0 41.0 33.0 41.0 42-43 37.86937185416904 40.0 37.0 41.0 33.0 41.0 44-45 37.720589507267086 40.0 37.0 41.0 33.0 41.0 46-47 37.56790895408287 40.0 36.0 41.0 32.0 41.0 48-49 37.42800937740871 39.5 36.0 41.0 32.0 41.0 50-51 37.31311787577546 39.0 36.0 41.0 32.0 41.0 52-53 37.25081493718136 39.0 35.5 41.0 32.0 41.0 54-55 37.03464440312946 39.0 35.0 41.0 31.5 41.0 56-57 36.75919597369676 39.0 35.0 41.0 31.0 41.0 58-59 36.47795739081351 38.0 35.0 40.0 31.0 41.0 60-61 36.17873309027873 38.0 35.0 40.0 31.0 41.0 62-63 35.83783988939559 37.0 35.0 40.0 30.5 41.0 64-65 35.51468024414779 37.0 34.5 40.0 30.0 41.0 66-67 35.17987620973573 36.0 34.0 39.0 30.0 41.0 68-69 34.83858702776778 36.0 34.0 39.0 29.5 41.0 70-71 34.51246202928819 35.0 34.0 38.5 29.0 40.5 72-73 34.1764803002789 35.0 34.0 37.0 29.0 40.0 74-75 33.88077072605091 35.0 34.0 37.0 29.0 39.0 76-77 33.679279985656386 35.0 34.0 36.5 29.0 39.0 78-79 33.57627129524557 35.0 34.0 36.0 29.0 39.0 80-81 33.36531439952104 35.0 34.0 36.0 29.0 37.0 82-83 33.1551661341119 35.0 34.0 35.5 29.0 37.0 84-85 32.97292541275059 35.0 34.0 35.0 29.0 36.5 86-87 32.78945890693904 35.0 33.0 35.0 29.0 36.0 88-89 32.65954884284777 35.0 33.0 35.0 29.0 36.0 90-91 32.53553885187109 35.0 33.0 35.0 29.0 36.0 92-93 32.40326742958722 35.0 33.0 35.0 29.0 36.0 94-95 32.29269590589796 35.0 33.0 35.0 29.0 35.0 96-97 32.177549426683356 35.0 33.0 35.0 29.0 35.0 98-99 32.030963671100544 35.0 33.0 35.0 28.5 35.0 100-101 31.57486813018993 34.5 32.5 35.0 26.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3165928943855221 1101 2 0.34821640962660894 1101 3 0.2962567566253327 1101 4 0.20023591171263888 1101 5 0.255390829864389 1101 6 0.2725245524916531 1101 7 0.24698795664956208 1101 8 0.294688710774075 1101 9 0.370695253524822 1101 10-11 0.3724296804234086 1101 12-13 0.3424913102977811 1101 14-15 0.4172688922298988 1101 16-17 0.3607649876092225 1101 18-19 0.4708984851366722 1101 20-21 0.42258342389977344 1101 22-23 0.5130423544634937 1101 24-25 0.5211545760399474 1101 26-27 0.5330045857295218 1101 28-29 0.5322442560664271 1101 30-31 0.5182905805932734 1101 32-33 0.5204015268223401 1101 34-35 0.47840948661749394 1101 36-37 0.4898013427768291 1101 38-39 0.4829815640645805 1101 40-41 0.43780818160112744 1101 42-43 0.3985575613581531 1101 44-45 0.4496253690001737 1101 46-47 0.5307508647851051 1101 48-49 0.4359684267749202 1101 50-51 0.4573066846980254 1101 52-53 0.37625159527385676 1101 54-55 0.3588709463272792 1101 56-57 0.3089267943747913 1101 58-59 0.29983019255851673 1101 60-61 0.28292553711318646 1101 62-63 0.24165507288419263 1101 64-65 0.17284286558813733 1101 66-67 0.2179430040636845 1101 68-69 0.18906828051463975 1101 70-71 0.13837077329369407 1101 72-73 0.11424557232246713 1101 74-75 0.1287391099348767 1101 76-77 0.18677537672361666 1101 78-79 0.1471425689527237 1101 80-81 0.07806535953512395 1101 82-83 0.10155827947784246 1101 84-85 0.09800710847769523 1101 86-87 0.12055741696979538 1101 88-89 0.08201144816077743 1101 90-91 0.08183328301146986 1101 92-93 0.11482289682010105 1101 94-95 0.12159618748243872 1101 96-97 0.15971698450368876 1101 98-99 0.19980785156087677 1101 100-101 0.16673831070676925 1102 1 0.30345246693839556 1102 2 0.32640463799097574 1102 3 0.3331207186187086 1102 4 0.25247274455807656 1102 5 0.24362953051100789 1102 6 0.26983543672424304 1102 7 0.26476402373242536 1102 8 0.27066875434891813 1102 9 0.34163994011158394 1102 10-11 0.36826671351350626 1102 12-13 0.372265966835279 1102 14-15 0.509221218866756 1102 16-17 0.4768979363114738 1102 18-19 0.4454722695668565 1102 20-21 0.49362144668751995 1102 22-23 0.5382477515543584 1102 24-25 0.49248426996759065 1102 26-27 0.4883194531325117 1102 28-29 0.5022032484591392 1102 30-31 0.4830149520056395 1102 32-33 0.5147152714621015 1102 34-35 0.4506284686227673 1102 36-37 0.45947331602871344 1102 38-39 0.4929734207099443 1102 40-41 0.5239528588165783 1102 42-43 0.4097215175527964 1102 44-45 0.48010055196533585 1102 46-47 0.4348442155277894 1102 48-49 0.46048886020388835 1102 50-51 0.4450070168772413 1102 52-53 0.3928237191670263 1102 54-55 0.3631545218667682 1102 56-57 0.453547896345178 1102 58-59 0.35701120500586114 1102 60-61 0.3508405829482797 1102 62-63 0.3963945757481966 1102 64-65 0.37490554132284615 1102 66-67 0.42889679896388344 1102 68-69 0.35614413085428964 1102 70-71 0.29493537230349176 1102 72-73 0.3224632489853363 1102 74-75 0.3634286247912897 1102 76-77 0.3216891839106637 1102 78-79 0.3249777884588525 1102 80-81 0.36748045940791485 1102 82-83 0.33528858040495635 1102 84-85 0.3340469709706255 1102 86-87 0.3607932906013076 1102 88-89 0.3275013092713337 1102 90-91 0.2652785710144272 1102 92-93 0.37426774525064843 1102 94-95 0.33113945946653445 1102 96-97 0.3998617031899201 1102 98-99 0.434157936549056 1102 100-101 0.4270399976701853 1103 1 0.2800821098493955 1103 2 0.3141943298665737 1103 3 0.23898789506188933 1103 4 0.18564966811425165 1103 5 0.25419243402115654 1103 6 0.25911907634592524 1103 7 0.24950223374921165 1103 8 0.23713165849625284 1103 9 0.3456317656571457 1103 10-11 0.3416503542679692 1103 12-13 0.3356707074666261 1103 14-15 0.41359747864675 1103 16-17 0.3848071333869072 1103 18-19 0.3985269462312857 1103 20-21 0.42771021732631453 1103 22-23 0.4789221582055063 1103 24-25 0.4560389496409769 1103 26-27 0.4644123952493615 1103 28-29 0.43202797133408666 1103 30-31 0.45310522163661915 1103 32-33 0.5244258875622307 1103 34-35 0.4892509914536731 1103 36-37 0.5463822061904153 1103 38-39 0.5143237491111776 1103 40-41 0.4408769831566417 1103 42-43 0.40348764202879295 1103 44-45 0.37328937241124294 1103 46-47 0.4453760559694615 1103 48-49 0.4562356535394372 1103 50-51 0.42218926823640146 1103 52-53 0.4506606525713508 1103 54-55 0.429823455576944 1103 56-57 0.444529451254418 1103 58-59 0.37887239256023975 1103 60-61 0.4069744017622696 1103 62-63 0.43303587660223997 1103 64-65 0.38082471521867944 1103 66-67 0.43625958693003497 1103 68-69 0.37471460224830366 1103 70-71 0.3674593404255262 1103 72-73 0.40935862095982856 1103 74-75 0.3563072172218824 1103 76-77 0.40596408516373117 1103 78-79 0.3249106178919874 1103 80-81 0.29443014484788677 1103 82-83 0.3277591935031978 1103 84-85 0.3801961814907884 1103 86-87 0.3953377237100426 1103 88-89 0.34750240754107864 1103 90-91 0.4442918044125932 1103 92-93 0.4278403653948146 1103 94-95 0.3983385229371379 1103 96-97 0.4223319835327928 1103 98-99 0.4286427595408995 1103 100-101 0.4295096222371626 1104 1 0.2821693735199915 1104 2 0.28015064944766266 1104 3 0.21606898983473855 1104 4 0.18009822709549894 1104 5 0.22767864669823723 1104 6 0.2001946547416864 1104 7 0.2016645141820277 1104 8 0.20507467279632152 1104 9 0.29377268360482844 1104 10-11 0.25931325394559934 1104 12-13 0.30223983911347574 1104 14-15 0.35236280592629043 1104 16-17 0.33821246088974277 1104 18-19 0.39387550138341254 1104 20-21 0.3720604359220445 1104 22-23 0.42023923728192614 1104 24-25 0.38246242552200727 1104 26-27 0.4177567097585637 1104 28-29 0.38359290010588865 1104 30-31 0.4069930489643525 1104 32-33 0.44641863822423744 1104 34-35 0.3321862101335853 1104 36-37 0.41076896542005414 1104 38-39 0.37947160073420605 1104 40-41 0.4208269969859657 1104 42-43 0.4388815892781537 1104 44-45 0.48235664030521264 1104 46-47 0.45947147488362816 1104 48-49 0.4696264138084416 1104 50-51 0.43341193025032965 1104 52-53 0.5081063268024337 1104 54-55 0.49881598059118204 1104 56-57 0.49204806283418634 1104 58-59 0.5129175034481932 1104 60-61 0.46776251083134923 1104 62-63 0.5023018697912889 1104 64-65 0.37936975811574314 1104 66-67 0.41927390095339945 1104 68-69 0.37226867463230207 1104 70-71 0.3063629173046465 1104 72-73 0.33531226626158883 1104 74-75 0.321052435421187 1104 76-77 0.29654478232724557 1104 78-79 0.2981382340388805 1104 80-81 0.2717094337233448 1104 82-83 0.3187346783166305 1104 84-85 0.36891876478529895 1104 86-87 0.3929667212713497 1104 88-89 0.3681048687677375 1104 90-91 0.3431074894358588 1104 92-93 0.39726132024860306 1104 94-95 0.4175904120503233 1104 96-97 0.38729757246605345 1104 98-99 0.465867009375458 1104 100-101 0.49465060935325766 1105 1 0.14015060224051723 1105 2 0.19934351671869877 1105 3 0.1691747970876918 1105 4 0.136606989979974 1105 5 0.16598478501104807 1105 6 0.14213792596208208 1105 7 0.16176476816175267 1105 8 0.11728773478743193 1105 9 0.19283527892451247 1105 10-11 0.21637281002723796 1105 12-13 0.21817397544500494 1105 14-15 0.30175719961754055 1105 16-17 0.23104370365918925 1105 18-19 0.22431367823904225 1105 20-21 0.22684826203558828 1105 22-23 0.3067889558569945 1105 24-25 0.2581136264126158 1105 26-27 0.24844104325585903 1105 28-29 0.16952245250526943 1105 30-31 0.23101743483078963 1105 32-33 0.2513019514745949 1105 34-35 0.22208152078300714 1105 36-37 0.21238742923772946 1105 38-39 0.24127074773467427 1105 40-41 0.26380756963227014 1105 42-43 0.17813586217617683 1105 44-45 0.23128637630232163 1105 46-47 0.13826440824297492 1105 48-49 0.2780106215180993 1105 50-51 0.2559331204170405 1105 52-53 0.223054101920809 1105 54-55 0.28358231082803087 1105 56-57 0.24194351174008943 1105 58-59 0.1645765725922388 1105 60-61 0.22935065783731545 1105 62-63 0.12646610509042233 1105 64-65 0.0928086484091395 1105 66-67 0.11630513936046327 1105 68-69 0.07538257547098226 1105 70-71 0.11097751062794003 1105 72-73 0.027825544894589882 1105 74-75 -0.015098353104455953 1105 76-77 0.06740871528263881 1105 78-79 0.04779270018682524 1105 80-81 0.06721318279247868 1105 82-83 0.00808244179054185 1105 84-85 0.037309808891350826 1105 86-87 0.052114589923235144 1105 88-89 0.055189863182519616 1105 90-91 0.04637731524300648 1105 92-93 0.009331656041808856 1105 94-95 -0.051357633913340806 1105 96-97 -0.013323757296767269 1105 98-99 0.013726313916180288 1105 100-101 0.029415758828260863 1106 1 0.1478863955588139 1106 2 0.18164357580130286 1106 3 0.14850662650008672 1106 4 0.1225369368056306 1106 5 0.0976943537080075 1106 6 0.12747335404243643 1106 7 0.14109827605733472 1106 8 0.13109896585414305 1106 9 0.25978762342982975 1106 10-11 0.20514073622769047 1106 12-13 0.2149774720611859 1106 14-15 0.22069283985816668 1106 16-17 0.29670546244101104 1106 18-19 0.2958489532417232 1106 20-21 0.29962712523249735 1106 22-23 0.31114881593863686 1106 24-25 0.26002860788216253 1106 26-27 0.2285174478105958 1106 28-29 0.2754444768902644 1106 30-31 0.2538983498055529 1106 32-33 0.2674983071519037 1106 34-35 0.21969085717788772 1106 36-37 0.2570594200530678 1106 38-39 0.2602777235108107 1106 40-41 0.2696010222050518 1106 42-43 0.27840962278418857 1106 44-45 0.16862910242757323 1106 46-47 0.145769242274568 1106 48-49 0.12243369327661213 1106 50-51 0.16235298291569933 1106 52-53 0.06868870284463924 1106 54-55 0.05443696089785277 1106 56-57 0.1669008782671071 1106 58-59 0.04645600378785275 1106 60-61 -0.02157972539396269 1106 62-63 0.08879523550184132 1106 64-65 0.022274756476605262 1106 66-67 0.11955703268949947 1106 68-69 0.0854308150778138 1106 70-71 0.08850345997803544 1106 72-73 0.01762825598063955 1106 74-75 0.039331499726138475 1106 76-77 0.05333026969235277 1106 78-79 0.09074860979043109 1106 80-81 0.05026805576486737 1106 82-83 0.05992424139307673 1106 84-85 0.036770512242945586 1106 86-87 0.060022092071690736 1106 88-89 0.09563476193641662 1106 90-91 0.042975365516937813 1106 92-93 0.06385399329607822 1106 94-95 0.07065986930933832 1106 96-97 0.12444815697973155 1106 98-99 0.14209452613016538 1106 100-101 0.12330608002280385 1107 1 0.004375193789122989 1107 2 0.07247099223348386 1107 3 0.04705589310691494 1107 4 0.035135271668615076 1107 5 0.039487132735281705 1107 6 0.0515603946841523 1107 7 0.09296121742958263 1107 8 0.046186559524407755 1107 9 0.07634355831702067 1107 10-11 -0.003975725028375621 1107 12-13 0.016698080022784723 1107 14-15 0.02304041661818701 1107 16-17 -0.06669902919296078 1107 18-19 -0.004196453091381613 1107 20-21 -0.04146903113506539 1107 22-23 0.06905699807160914 1107 24-25 -0.019048506821675915 1107 26-27 -0.009214857797353204 1107 28-29 0.05571493138224071 1107 30-31 0.10135792745310823 1107 32-33 0.06856620695456428 1107 34-35 0.024675468192072003 1107 36-37 0.012204665688514638 1107 38-39 -0.004442396138344407 1107 40-41 0.04843650209599559 1107 42-43 0.02121203610663258 1107 44-45 0.053824746781472754 1107 46-47 0.05315658582127014 1107 48-49 0.017779134405131458 1107 50-51 0.08259267204306298 1107 52-53 0.1892381257786866 1107 54-55 0.02914481390320134 1107 56-57 0.10278545499014768 1107 58-59 -0.04843972343627456 1107 60-61 0.026522390738662693 1107 62-63 -0.13433240987405526 1107 64-65 -0.05752488834123426 1107 66-67 -0.059712717489517786 1107 68-69 -0.04517710869502167 1107 70-71 0.014706402123515261 1107 72-73 -0.16836312332970493 1107 74-75 -0.11511495904213831 1107 76-77 -0.13222429691144555 1107 78-79 -0.22704451635051015 1107 80-81 -0.18428790769826975 1107 82-83 -0.17493393367995935 1107 84-85 -0.14980299684925313 1107 86-87 -0.2412086485831395 1107 88-89 -0.20240681942265581 1107 90-91 -0.20293094152989255 1107 92-93 -0.18852375629797535 1107 94-95 -0.14958160581446123 1107 96-97 -0.12343966059143696 1107 98-99 -0.20610147038020799 1107 100-101 -0.2429615109915737 1108 1 -0.03982532769077096 1108 2 0.010049840144446875 1108 3 -0.004521780936386222 1108 4 -0.0020356067851778903 1108 5 -0.06272937656940769 1108 6 -0.03429955673274776 1108 7 -0.012307988285805038 1108 8 -0.05551336344706925 1108 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-0.1817465563060523 2214 88-89 -0.1718568987736191 2214 90-91 -0.20340582424915254 2214 92-93 -0.18548620660040172 2214 94-95 -0.16421880018364732 2214 96-97 -0.21238788354182248 2214 98-99 -0.3118875361803539 2214 100-101 -0.5513516701764374 2215 1 -0.47694921966063575 2215 2 -0.8667261936982591 2215 3 -0.503201253823967 2215 4 -0.32113563166343084 2215 5 -0.2856657776501308 2215 6 -0.2773345525598998 2215 7 -0.2862578503578277 2215 8 -0.3500547670753704 2215 9 -0.44202314550923916 2215 10-11 -0.4789053434853088 2215 12-13 -0.36609592916324374 2215 14-15 -0.5345397499495235 2215 16-17 -0.4485788855998365 2215 18-19 -0.42262173608081355 2215 20-21 -0.41710369869138475 2215 22-23 -0.5274463064466346 2215 24-25 -0.4903991210257743 2215 26-27 -0.47279153902864124 2215 28-29 -0.4413428744420216 2215 30-31 -0.48813264733877304 2215 32-33 -0.438924320775925 2215 34-35 -0.5128361030071886 2215 36-37 -0.43937015373983 2215 38-39 -0.3964843079499474 2215 40-41 -0.4991708174575251 2215 42-43 -0.4208897096975974 2215 44-45 -0.37260010571469593 2215 46-47 -0.38471433213704387 2215 48-49 -0.44168749367006654 2215 50-51 -0.38238224469911586 2215 52-53 -0.40703243221074814 2215 54-55 -0.42093010343570114 2215 56-57 -0.31399693248602745 2215 58-59 -0.31390494627176935 2215 60-61 -0.3113930768393516 2215 62-63 -0.28966129390238393 2215 64-65 -0.23050511760386883 2215 66-67 -0.20668810873920052 2215 68-69 -0.19417481737220754 2215 70-71 -0.20475804259075403 2215 72-73 -0.18543735281892992 2215 74-75 -0.224994840757212 2215 76-77 -0.14575574926459467 2215 78-79 -0.09304474937211893 2215 80-81 -0.08402164897037068 2215 82-83 -0.16492643602106938 2215 84-85 -0.1780197521531477 2215 86-87 -0.1507519820763008 2215 88-89 -0.14600236245465226 2215 90-91 -0.16178357076156402 2215 92-93 -0.23963485381921146 2215 94-95 -0.23125493168258515 2215 96-97 -0.18422854033937952 2215 98-99 -0.2373952240931203 2215 100-101 -0.14388776345843723 2216 1 -0.47044921785762384 2216 2 -0.49363443794175055 2216 3 -0.4809458736892367 2216 4 -0.27812054203158 2216 5 -0.3943877901956938 2216 6 -0.3321464153481486 2216 7 -0.3736098650740445 2216 8 -0.4036885172304352 2216 9 -0.4696048290835293 2216 10-11 -0.5829024709355579 2216 12-13 -0.6502340570925966 2216 14-15 -0.8944908879213145 2216 16-17 -1.194217193353687 2216 18-19 -1.2278775956508312 2216 20-21 -1.53377922014824 2216 22-23 -1.3367674597390504 2216 24-25 -1.3736473964244738 2216 26-27 -1.2616557644231605 2216 28-29 -1.1999286912234695 2216 30-31 -1.223294090031537 2216 32-33 -1.0564135910954846 2216 34-35 -0.5996087292792396 2216 36-37 -0.9602441265050743 2216 38-39 -1.225945121275629 2216 40-41 -1.2989568787356234 2216 42-43 -0.6490763057282152 2216 44-45 -1.0898297589466637 2216 46-47 -0.7603661156408634 2216 48-49 -0.8300238988239244 2216 50-51 -0.9450627815953396 2216 52-53 -0.9669157002674638 2216 54-55 -1.166832428039875 2216 56-57 -1.1490846444398883 2216 58-59 -0.8312967026790261 2216 60-61 -0.9244603058356304 2216 62-63 -0.8317751886100737 2216 64-65 -0.9253320411343324 2216 66-67 -0.6923778441860122 2216 68-69 -0.7374379039928982 2216 70-71 -0.710571521830694 2216 72-73 -0.6170168042234323 2216 74-75 -0.6639895606689024 2216 76-77 -0.591684209246921 2216 78-79 -0.4555853746424461 2216 80-81 -0.48588854725702646 2216 82-83 -0.686927000881802 2216 84-85 -0.5820404203028104 2216 86-87 -0.6637442348049731 2216 88-89 -0.5851026103542409 2216 90-91 -0.6239583877797159 2216 92-93 -0.5379996031440264 2216 94-95 -0.6345595866315499 2216 96-97 -0.6468768758633381 2216 98-99 -0.8511055375804588 2216 100-101 -0.6456327470761067 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 37.0 13 151.0 14 596.0 15 1488.0 16 2769.0 17 4709.0 18 8938.0 19 18408.0 20 20242.0 21 18240.0 22 17340.0 23 17250.0 24 18984.0 25 22576.0 26 28773.0 27 38057.0 28 48857.0 29 62173.0 30 79666.0 31 104006.0 32 135006.0 33 179124.0 34 244230.0 35 348794.0 36 609866.0 37 1113122.0 38 1057151.0 39 238743.0 40 308.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.240774519180306 39.3213271628158 13.76804608336866 20.669852234635236 2 23.94530055144533 28.284289191428247 30.265658709353936 17.504751547772482 3 21.95075418854736 27.56999157132412 24.957372343401644 25.52188189672687 4 23.77451957949728 26.611400929868665 26.166904508080634 23.447174982553413 5 23.682416863118032 27.44757079794919 25.15820097549197 23.71181136344081 6 23.82240676312267 26.08472463547441 25.97982793968654 24.11304066171638 7 24.41827045012553 27.287376402320383 24.617499841202125 23.676853306351962 8 24.611981333478692 25.788482131071994 26.030800931434005 23.568735604015313 9 23.259293730119293 25.685950510022735 26.623916626835804 24.430839133022165 10-11 24.533776195455182 26.158560016361815 25.392816389562494 23.91484739862051 12-13 24.019991864142902 26.238567116090934 25.563191868828 24.178249150938168 14-15 23.833319893702278 26.139538058106957 25.758040240507828 24.269101807682933 16-17 24.006015398663756 26.45282036288806 25.638221049345372 23.902943189102814 18-19 23.198390037132803 26.351868426147323 26.729210512060135 23.720531024659735 20-21 22.616567776509896 26.831142222981047 26.632261961984916 23.920028038524137 22-23 22.371263292852245 26.656780649805707 27.546927608858805 23.425028448483243 24-25 22.264914949659946 26.047097422609124 28.84322392572674 22.844763702004187 26-27 22.31316880283764 25.754051179131466 29.393611252358742 22.53916876567215 28-29 22.333668761638865 25.945538981855897 29.32335645292169 22.397435803583544 30-31 22.319733399705605 25.914692861189227 29.361666184266394 22.403907554838774 32-33 22.282137455681593 25.880621600275074 29.348812636618277 22.488428307425064 34-35 22.516164722725396 26.106990124799363 29.157362162318005 22.219482990157235 36-37 22.36501021656321 26.143939172960113 29.006232424532413 22.484818185944267 38-39 22.466668476137567 26.10361488362643 29.004684765204818 22.42503187503118 40-41 22.56887753751717 26.30585792573003 28.432761010816176 22.692503525936626 42-43 22.702396916658813 26.463851074854837 28.172991730447755 22.660760278038595 44-45 22.6625897113189 26.39300385452477 28.1911438844262 22.753262549730128 46-47 22.76452577208242 26.830206907130496 27.83399091877749 22.571276402009595 48-49 22.74987558013332 26.915550698266028 27.61479091211841 22.719782809482243 50-51 22.7576000158573 26.587655723628906 27.766287743299166 22.888456517214628 52-53 22.82216726914951 26.898778264576855 27.5971605651975 22.681893901076137 54-55 22.73936597045223 26.820190259995563 27.550736205294378 22.889707564257826 56-57 22.801331875995658 26.905708337627072 27.485681432065146 22.80727835431213 58-59 23.00737607793755 26.972695120647806 27.250639675670158 22.769289125744486 60-61 22.845265942650045 27.037268123034025 27.203961631548896 22.913504302767038 62-63 22.824462247882238 26.814840192522194 27.402518069227984 22.958179490367584 64-65 22.968999213216875 27.124421428341357 27.12214644707617 22.784432911365606 66-67 22.894473423518594 26.82755120841913 27.252848340909843 23.025127027152433 68-69 23.00138920877043 26.968151811743347 26.93362149889263 23.096837480593592 70-71 23.078060570758367 27.006085817265106 26.973571513280127 22.9422820986964 72-73 22.92505823440645 26.999073452667645 26.845760108370108 23.230108204555794 74-75 22.995375003575795 26.98342925476358 26.91249897794179 23.108696763718836 76-77 22.994661199475445 27.15665962545055 26.87417756748733 22.974501607586674 78-79 23.088716408443418 27.122141480490985 26.66351923703062 23.125622874034978 80-81 22.940599445220915 27.22724206320157 26.684896516694618 23.147261974882902 82-83 23.008726965814176 27.34724093203414 26.510888406629057 23.13314369552263 84-85 23.083343431827057 27.27254400503108 26.621224946538046 23.022887616603814 86-87 23.06786301934519 27.11672547445099 26.759193667004162 23.056217839199658 88-89 23.133442502573438 27.293669009976146 26.570426548397485 23.00246193905293 90-91 23.1470222309918 27.244213176868453 26.605911063193776 23.00285352894597 92-93 23.0703337628776 27.14155522053956 26.654956123190683 23.133154893392156 94-95 23.193004063874138 27.448111386460873 26.36923186426904 22.989652685395956 96-97 23.257503823835847 27.318771691913202 26.376837122105854 23.046887362145103 98-99 23.181534833355013 27.259208453910006 26.503802986866503 23.055453725868478 100-101 23.261740805825916 27.445301481326467 26.203590424124002 23.089367288723615 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2119.0 1 1562.0 2 761.5 3 394.5 4 231.5 5 186.5 6 170.0 7 164.0 8 158.0 9 139.0 10 148.0 11 152.5 12 175.0 13 328.0 14 463.0 15 629.0 16 3143.5 17 3708.5 18 1997.0 19 2257.5 20 2859.0 21 3795.0 22 4963.5 23 6439.5 24 8174.0 25 10518.5 26 13708.5 27 17833.0 28 22845.5 29 28808.5 30 36958.0 31 46216.5 32 55198.5 33 66627.5 34 78648.0 35 89930.5 36 101244.0 37 112791.0 38 127436.0 39 145572.0 40 152968.0 41 150131.0 42 148835.0 43 153408.0 44 163597.5 45 175419.0 46 186392.5 47 191568.5 48 189196.5 49 183382.0 50 178259.5 51 177498.0 52 184532.0 53 183648.5 54 162773.5 55 144265.0 56 132231.0 57 109584.5 58 91315.5 59 83362.0 60 73901.0 61 61300.5 62 46459.0 63 34199.0 64 24685.0 65 15794.0 66 10240.5 67 7101.5 68 5119.5 69 3865.0 70 3173.0 71 2794.5 72 2678.5 73 2145.0 74 1490.5 75 1231.0 76 1010.5 77 730.0 78 470.5 79 266.0 80 88.5 81 40.0 82 41.5 83 15.5 84 1.0 85 0.5 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.03998102534323992 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 1.1262260660067583E-5 20-21 0.0 22-23 4.504904264027033E-5 24-25 0.0 26-27 7.883582462047309E-5 28-29 1.9145843122114889E-4 30-31 2.2524521320135166E-5 32-33 1.1262260660067583E-5 34-35 0.0 36-37 3.378678198020274E-5 38-39 5.631130330033791E-5 40-41 1.238848672607434E-4 42-43 1.4640938858087856E-4 44-45 4.1670364442250054E-4 46-47 1.238848672607434E-4 48-49 3.378678198020274E-5 50-51 2.2524521320135165E-4 52-53 0.0013627335398681774 54-55 0.0011149638053466907 56-57 7.883582462047309E-5 58-59 0.001160012847986961 60-61 4.730149477228384E-4 62-63 3.1534329848189235E-4 64-65 2.027206918812165E-4 66-67 9.009808528054066E-5 68-69 6.419488576238522E-4 70-71 4.8427720838290606E-4 72-73 1.238848672607434E-4 74-75 7.883582462047307E-4 76-77 7.207846822443253E-4 78-79 1.0136034594060825E-4 80-81 2.2524521320135166E-5 82-83 0.0020384691794722324 84-85 0.0 86-87 2.2524521320135166E-5 88-89 3.6039234112216265E-4 90-91 3.4913008046209506E-4 92-93 3.8291686244229777E-4 94-95 0.0 96-97 4.3922816574263577E-4 98-99 7.883582462047309E-5 100-101 0.009212529219935282 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4439606.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.83085161034954 #Duplication Level Percentage of deduplicated Percentage of total 1 82.52308097643902 37.82103078660036 2 9.408945499531578 8.624399699977957 3 2.8167071809686712 3.8727626652234335 4 1.297726929784714 2.3790372139887093 5 0.8004350300662201 1.83423095433453 6 0.5139288030941315 1.4132276827735013 7 0.37877417848226635 1.2151680217496958 8 0.2883754036070217 1.0573192264630453 9 0.22632493486185049 0.9335398054837947 >10 1.3492661417039162 11.901523148997793 >50 0.17924445605994102 5.82393339116816 >100 0.20685105939742673 18.93498443179362 >500 0.009650112285666824 2.767860910907953 >1k 5.41587920983042E-4 0.4797757859899824 >5k 4.923526554391286E-5 0.1159612343242648 >10k+ 9.847053108782572E-5 0.8252450402231977 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19466 0.4384623320177511 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 17106 0.3853044617022321 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 5139 0.11575351506417461 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4862 0.10951422265849718 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 4.504904264027033E-5 0.0 0.0 0.0 0.0 4 4.504904264027033E-5 0.0 0.0 0.0 0.0 5 6.75735639604055E-5 0.0 0.0 0.0 0.0 6 2.477697345214868E-4 0.0 0.0 0.0 0.0 7 3.829168624422978E-4 0.0 0.0 0.0 0.0 8 9.009808528054066E-4 0.0 0.0 0.0 0.0 9 0.0018695352695712187 0.0 0.0 0.0 0.0 10-11 0.005664917112013994 0.0 0.0 0.0 0.0 12-13 0.010361279807262177 0.0 0.0 0.0 1.1262260660067583E-5 14-15 0.014888708592609344 0.0 0.0 0.0 2.2524521320135166E-5 16-17 0.018492632003830972 0.0 0.0 0.0 2.2524521320135166E-5 18-19 0.02106042743432638 0.0 0.0 0.0 3.378678198020275E-5 20-21 0.02445036789300672 2.2524521320135166E-5 0.0 0.0 6.75735639604055E-5 22-23 0.02752496505320517 2.2524521320135166E-5 0.0 0.0 6.75735639604055E-5 24-25 0.031297822374327815 2.2524521320135166E-5 0.0 0.0 6.75735639604055E-5 26-27 0.03630952836805788 3.378678198020275E-5 0.0 0.0 6.75735639604055E-5 28-29 0.04038646672700235 4.504904264027033E-5 0.0 0.0 6.75735639604055E-5 30-31 0.04428320891538573 4.504904264027033E-5 0.0 0.0 7.883582462047309E-5 32-33 0.04927239038779567 4.504904264027033E-5 0.0 0.0 9.009808528054066E-5 34-35 0.05506119236707041 4.504904264027033E-5 0.0 0.0 9.009808528054066E-5 36-37 0.06122164894812737 4.504904264027033E-5 0.0 0.0 9.009808528054066E-5 38-39 0.06851959385585117 4.504904264027033E-5 0.0 0.0 9.009808528054066E-5 40-41 0.07759697594786563 4.504904264027033E-5 0.0 0.0 9.009808528054066E-5 42-43 0.08928720251301579 4.504904264027033E-5 0.0 0.0 9.009808528054066E-5 44-45 0.10308347182159858 4.504904264027033E-5 0.0 0.0 9.009808528054066E-5 46-47 0.11825373693070962 4.504904264027033E-5 0.0 0.0 1.1262260660067582E-4 48-49 0.1360143219916362 4.504904264027033E-5 0.0 0.0 1.1262260660067582E-4 50-51 0.15752523985236527 4.504904264027033E-5 0.0 0.0 1.1262260660067582E-4 52-53 0.18291037538015759 4.504904264027033E-5 0.0 0.0 1.35147127920811E-4 54-55 0.2127103170866964 4.504904264027033E-5 0.0 0.0 1.35147127920811E-4 56-57 0.24778099678214688 4.504904264027033E-5 0.0 0.0 1.35147127920811E-4 58-59 0.286793467708621 4.504904264027033E-5 0.0 0.0 1.5767164924094615E-4 60-61 0.33111046340598693 4.504904264027033E-5 0.0 0.0 1.8019617056108132E-4 62-63 0.3816554892483702 4.504904264027033E-5 0.0 0.0 1.8019617056108132E-4 64-65 0.4381244641979491 4.504904264027033E-5 0.0 0.0 2.0272069188121647E-4 66-67 0.501767499187991 4.504904264027033E-5 0.0 0.0 2.0272069188121647E-4 68-69 0.5740036390616645 4.504904264027033E-5 0.0 0.0 2.0272069188121647E-4 70-71 0.6546639499090685 4.504904264027033E-5 0.0 0.0 2.0272069188121647E-4 72-73 0.7425996811428762 4.504904264027033E-5 0.0 0.0 2.0272069188121647E-4 74-75 0.8406489224494246 4.504904264027033E-5 0.0 0.0 2.1398295254128406E-4 76-77 0.9507938317048855 4.504904264027033E-5 0.0 0.0 2.2524521320135165E-4 78-79 1.0723811977909752 4.504904264027033E-5 0.0 0.0 2.2524521320135165E-4 80-81 1.2064921977310599 4.504904264027033E-5 0.0 0.0 2.2524521320135165E-4 82-83 1.3507617567865258 4.504904264027033E-5 0.0 0.0 2.3650747386141924E-4 84-85 1.511305282495789 4.504904264027033E-5 0.0 0.0 2.477697345214868E-4 86-87 1.6830322330404996 4.504904264027033E-5 0.0 0.0 2.477697345214868E-4 88-89 1.8688482716709545 4.504904264027033E-5 0.0 0.0 2.477697345214868E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATATCTC 4065 0.0 70.95062 1 TCGACGG 7350 0.0 59.21561 2 TATCTCG 4890 0.0 58.883255 2 CGACGGC 7510 0.0 57.89076 3 GTCGACG 7405 0.0 57.364147 1 GGTCGAC 435 0.0 52.430027 1 CGGCGCG 9030 0.0 48.18247 6 GACGGCG 9930 0.0 44.021683 4 ATCTCGA 8715 0.0 43.3984 1 ACGGCGC 10070 0.0 43.395 5 TCTCGAG 8150 0.0 40.518677 2 CTCGAGG 9120 0.0 36.104923 3 TCGAGGG 9230 0.0 35.72612 4 CGAGGGC 9505 0.0 34.730736 5 GGGCGCG 9945 0.0 33.09861 8 AGGGCGC 10000 0.0 33.059063 7 GAGGGCG 10110 0.0 32.558426 6 CGCGCCG 18840 0.0 27.531065 9 GCGCGCC 19500 0.0 26.69668 8 GGCGCGC 19565 0.0 26.632265 7 >>END_MODULE