FastQCFastQC Report
Tue 31 May 2016
SRR2048258_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048258_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5056826
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT131760.26055869828228223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG169400.090.0289151
TATCTCG65700.082.456392
TCGACGG185450.082.3576352
ATATCTC68200.079.535741
CGACGGC194050.078.65873
CGGCGCG199750.076.15066
ATCTCGA72300.074.79783
ACGGCGC204350.074.5005955
GACGGCG204850.074.3710254
TCTCGAG57600.071.279974
TCGAGGG60250.068.202116
AGGGCGC60900.067.146899
CTCGAGG61600.066.723275
CGAGGGC61900.066.073237
CGCGCCG251650.060.9412169
CTCGACG21550.059.9740685
GCGCGCC258650.059.314988
GGCGCGC260250.058.9008067
GAGGGCG73250.056.024828
TCTCGAC26500.048.7752274
Produced by FastQC (version 0.11.5)