##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2048258_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5056826
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.96367444717299	31.0	31.0	34.0	28.0	34.0
2	31.14954973732535	31.0	31.0	34.0	28.0	34.0
3	31.35707516928603	31.0	31.0	34.0	28.0	34.0
4	35.069332225391975	37.0	35.0	37.0	32.0	37.0
5	34.944813999928016	37.0	35.0	37.0	32.0	37.0
6	35.05987945798412	37.0	35.0	37.0	32.0	37.0
7	35.01091415840688	37.0	35.0	37.0	32.0	37.0
8	35.02312873727512	37.0	35.0	37.0	32.0	37.0
9	36.57947000747109	39.0	37.0	39.0	32.0	39.0
10-11	36.516749043767774	39.0	37.0	39.0	32.0	39.0
12-13	36.49282455041957	39.0	37.0	39.0	32.0	39.0
14-15	37.65554648311016	40.0	37.0	41.0	32.0	41.0
16-17	37.66605396349409	40.0	37.0	41.0	32.0	41.0
18-19	37.68244695387977	40.0	37.0	41.0	32.0	41.0
20-21	37.64613613361425	40.0	37.0	41.0	31.5	41.0
22-23	37.68775532320076	40.0	37.0	41.0	32.0	41.0
24-25	37.664431503081175	40.0	37.0	41.0	32.0	41.0
26-27	37.67158668303003	40.0	37.0	41.0	32.0	41.0
28-29	37.46653849667756	40.0	37.0	41.0	31.0	41.0
30-31	37.32806398717298	40.0	36.5	41.0	31.0	41.0
32-33	37.25837056683382	40.0	36.0	41.0	31.0	41.0
34-35	36.99087935792136	39.0	35.0	41.0	30.5	41.0
36-37	36.901687046380474	39.0	35.0	41.0	30.0	41.0
38-39	36.77502953433636	39.0	35.0	41.0	30.0	41.0
40-41	36.39370654635932	39.0	35.0	40.5	29.0	41.0
42-43	36.177680228665174	38.5	35.0	40.0	28.5	41.0
44-45	36.01842242545027	38.0	34.5	40.0	28.0	41.0
46-47	35.9302375640372	38.0	34.0	40.0	28.0	41.0
48-49	35.812513224698655	38.0	34.0	40.0	28.0	41.0
50-51	35.75933461028716	38.0	34.0	40.0	28.0	41.0
52-53	35.56860855010633	38.0	34.0	40.0	27.5	41.0
54-55	35.4946517835496	38.0	34.0	40.0	28.0	41.0
56-57	35.40510282536911	38.0	34.0	40.0	28.0	41.0
58-59	35.271548694774154	37.5	33.5	40.0	28.0	41.0
60-61	35.01637311625909	37.0	33.0	40.0	27.0	41.0
62-63	34.69530412950732	37.0	33.0	40.0	26.5	41.0
64-65	34.34054266450932	36.0	33.0	39.0	26.0	41.0
66-67	34.0001124222981	36.0	32.5	39.0	26.0	41.0
68-69	33.64677754385854	35.0	32.0	39.0	26.0	40.0
70-71	33.26775857029686	35.0	32.0	38.0	26.0	40.0
72-73	32.99422355050382	35.0	32.0	37.0	25.5	39.5
74-75	32.97641079602106	35.0	32.0	37.0	26.0	39.0
76-77	32.71735669765975	35.0	32.0	36.5	26.0	39.0
78-79	32.427918718184095	35.0	32.0	36.0	26.0	38.5
80-81	32.157029429132024	35.0	32.0	36.0	25.5	37.0
82-83	31.88972440420137	35.0	32.0	35.0	25.0	37.0
84-85	31.676131332183466	35.0	32.0	35.0	25.0	36.5
86-87	31.482519766351462	35.0	32.0	35.0	25.0	36.0
88-89	31.31098449897228	35.0	32.0	35.0	24.5	36.0
90-91	31.13474786358083	35.0	32.0	35.0	24.0	36.0
92-93	30.97720892512418	34.0	32.0	35.0	24.0	35.0
94-95	30.822835905368308	34.0	31.5	35.0	23.0	35.0
96-97	30.647861128700097	34.0	31.0	35.0	21.5	35.0
98-99	30.457402133274904	34.0	31.0	35.0	19.5	35.0
100-101	29.832821022514914	34.0	30.5	35.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5235667756628111
1101	2	0.5032342273186465
1101	3	0.5056095705470511
1101	4	0.43629826871565314
1101	5	0.45992417940165353
1101	6	0.457974093224486
1101	7	0.49325925551572425
1101	8	0.5604013485691581
1101	9	0.6339400481863535
1101	10-11	0.6413477947121962
1101	12-13	0.6403916350183891
1101	14-15	0.6827072941545609
1101	16-17	0.7022365929718362
1101	18-19	0.7495416849053314
1101	20-21	0.7788481596986543
1101	22-23	0.6993321996453474
1101	24-25	0.6608428341190873
1101	26-27	0.7139259807144498
1101	28-29	0.7936592012316765
1101	30-31	0.7221702943141253
1101	32-33	0.7292437642313558
1101	34-35	0.8235989866364335
1101	36-37	0.7960751004140079
1101	38-39	0.7003386059678007
1101	40-41	0.6280245342077109
1101	42-43	0.672969469309983
1101	44-45	0.6058706216934766
1101	46-47	0.5734348444263162
1101	48-49	0.574346754595318
1101	50-51	0.531473814696291
1101	52-53	0.5507266606314971
1101	54-55	0.42372232109112673
1101	56-57	0.44290995903011066
1101	58-59	0.4479564370443754
1101	60-61	0.3375797358720618
1101	62-63	0.2964182198247016
1101	64-65	0.27792462811274277
1101	66-67	0.36133960684073685
1101	68-69	0.43360062072721206
1101	70-71	0.3692826965300995
1101	72-73	0.38348623070668
1101	74-75	0.3653257860895778
1101	76-77	0.36271172929060924
1101	78-79	0.43930828088875273
1101	80-81	0.3641148672829857
1101	82-83	0.41902953990181757
1101	84-85	0.3653032206000688
1101	86-87	0.37883225015271194
1101	88-89	0.4169412778154573
1101	90-91	0.39768467427940024
1101	92-93	0.3832166967209609
1101	94-95	0.44079732967227514
1101	96-97	0.466300593301213
1101	98-99	0.3985789609147865
1101	100-101	0.41950979593285354
1102	1	0.37407911929528836
1102	2	0.40898918494562153
1102	3	0.46130421823529844
1102	4	0.41723162316454676
1102	5	0.3852818491488321
1102	6	0.39812733568074066
1102	7	0.4341785695065852
1102	8	0.45908282506136544
1102	9	0.5295336679305649
1102	10-11	0.5084710310342047
1102	12-13	0.5564483119476265
1102	14-15	0.7323068761762102
1102	16-17	0.5968039558587179
1102	18-19	0.6603384101691105
1102	20-21	0.7061275734899368
1102	22-23	0.6814687063274363
1102	24-25	0.6227513305185539
1102	26-27	0.5830536987304313
1102	28-29	0.7265844084806758
1102	30-31	0.7324494763366545
1102	32-33	0.697183272319684
1102	34-35	0.7277608792747827
1102	36-37	0.8123847685524694
1102	38-39	0.8019563140910719
1102	40-41	0.574161540237526
1102	42-43	0.6562986523988101
1102	44-45	0.683447403755828
1102	46-47	0.5552432956140336
1102	48-49	0.5897014745421814
1102	50-51	0.5701032288251824
1102	52-53	0.5661901385421046
1102	54-55	0.4856414928566082
1102	56-57	0.39715580704407216
1102	58-59	0.39725271538409146
1102	60-61	0.3480014550538968
1102	62-63	0.3693377042080854
1102	64-65	0.21259618948461423
1102	66-67	0.24875307593507756
1102	68-69	0.22550318278477022
1102	70-71	0.3177851370502509
1102	72-73	0.2823997966400569
1102	74-75	0.22743393428182657
1102	76-77	0.30745162886128696
1102	78-79	0.30093919104761824
1102	80-81	0.2673359398426456
1102	82-83	0.33437961520928994
1102	84-85	0.3074269428543417
1102	86-87	0.285293934113664
1102	88-89	0.2725346378019289
1102	90-91	0.2659802232842381
1102	92-93	0.29236808475994636
1102	94-95	0.19741498318940742
1102	96-97	0.23993083756808886
1102	98-99	0.2549990649308391
1102	100-101	0.2263998093577655
1103	1	0.30250823625336665
1103	2	0.2957347541258848
1103	3	0.3471443752608643
1103	4	0.27969852687837715
1103	5	0.2581221847896913
1103	6	0.3157445902867977
1103	7	0.3046218134623331
1103	8	0.3905528386577686
1103	9	0.4680441618486455
1103	10-11	0.42851752146642497
1103	12-13	0.4331567665196161
1103	14-15	0.5444443455061787
1103	16-17	0.5154700924906876
1103	18-19	0.5490204920349058
1103	20-21	0.6433543287174928
1103	22-23	0.478908117100886
1103	24-25	0.467163779291063
1103	26-27	0.49895136793135464
1103	28-29	0.6031669049963142
1103	30-31	0.5836141781829127
1103	32-33	0.6127364729000604
1103	34-35	0.6531628880826403
1103	36-37	0.6732692757933094
1103	38-39	0.6768381550123905
1103	40-41	0.5196882519578452
1103	42-43	0.600357503440712
1103	44-45	0.5500289054124821
1103	46-47	0.5625759874912433
1103	48-49	0.5310400961130242
1103	50-51	0.45828300085388207
1103	52-53	0.4442525617506661
1103	54-55	0.4025874003306811
1103	56-57	0.43197235716887405
1103	58-59	0.45448153514414713
1103	60-61	0.2906506354470153
1103	62-63	0.2505950288530556
1103	64-65	0.2096054968619825
1103	66-67	0.3112711771316867
1103	68-69	0.27733274377914796
1103	70-71	0.28310501031377555
1103	72-73	0.3064583682584754
1103	74-75	0.3326848306553458
1103	76-77	0.3785206718316445
1103	78-79	0.3345782216868045
1103	80-81	0.3615372290114678
1103	82-83	0.34124429013276014
1103	84-85	0.37897548593571884
1103	86-87	0.35432656962944975
1103	88-89	0.33144903716268814
1103	90-91	0.32980480330254736
1103	92-93	0.3552704976531551
1103	94-95	0.37456021356781477
1103	96-97	0.38986767498338537
1103	98-99	0.42654210653595115
1103	100-101	0.478808775671439
1104	1	0.20516952423206902
1104	2	0.21268975202421103
1104	3	0.25883457851455205
1104	4	0.2109572033788396
1104	5	0.15749434638836846
1104	6	0.2559562045360977
1104	7	0.29768126981686294
1104	8	0.31787915617155704
1104	9	0.386481462422374
1104	10-11	0.33348618056849944
1104	12-13	0.38313760543206854
1104	14-15	0.45570913691772574
1104	16-17	0.43269481734242277
1104	18-19	0.4885879812110474
1104	20-21	0.5421482477942234
1104	22-23	0.5169627122071887
1104	24-25	0.3730647796315054
1104	26-27	0.37567800015241204
1104	28-29	0.5387179496856191
1104	30-31	0.46699831587385887
1104	32-33	0.48236189224565607
1104	34-35	0.5037983377077708
1104	36-37	0.5363668667365786
1104	38-39	0.6151793025916277
1104	40-41	0.5355190165312109
1104	42-43	0.4934261319934947
1104	44-45	0.4311297264783036
1104	46-47	0.46267791726988605
1104	48-49	0.4880748892734701
1104	50-51	0.39906464019765764
1104	52-53	0.4136046724895266
1104	54-55	0.329902637788571
1104	56-57	0.20628300172468528
1104	58-59	0.2267881040579951
1104	60-61	0.2637809947294514
1104	62-63	0.315498114904571
1104	64-65	0.25536675917469154
1104	66-67	0.2550578606624896
1104	68-69	0.19979133004955685
1104	70-71	0.34099350325812594
1104	72-73	0.28363335207873064
1104	74-75	0.2599981484682914
1104	76-77	0.258113611795153
1104	78-79	0.2574860132932528
1104	80-81	0.2747900821848006
1104	82-83	0.3115680190558585
1104	84-85	0.29111815287014764
1104	86-87	0.2876122059602615
1104	88-89	0.31652735918150654
1104	90-91	0.2799300113019747
1104	92-93	0.3525702560372146
1104	94-95	0.34603648721097713
1104	96-97	0.38638744289764304
1104	98-99	0.37670445292941324
1104	100-101	0.4546135539775378
1105	1	0.14706098510821874
1105	2	0.23484604414376875
1105	3	0.23545258945229364
1105	4	0.2105112357330654
1105	5	0.13846464888258936
1105	6	0.20947265884515787
1105	7	0.20002424523783446
1105	8	0.20177557174478267
1105	9	0.2871534666998059
1105	10-11	0.2737182029507892
1105	12-13	0.3081194858810363
1105	14-15	0.2525150354395862
1105	16-17	0.35199063920130413
1105	18-19	0.30619516394009594
1105	20-21	0.39890440088797163
1105	22-23	0.36535006638685985
1105	24-25	0.2848674336566077
1105	26-27	0.43261181053622977
1105	28-29	0.47164445538654576
1105	30-31	0.44834343361040396
1105	32-33	0.36410762137609254
1105	34-35	0.3987943796231477
1105	36-37	0.5502850578015952
1105	38-39	0.45120201350761135
1105	40-41	0.40306969670657367
1105	42-43	0.3952156227826791
1105	44-45	0.32460231114932725
1105	46-47	0.408395270699657
1105	48-49	0.4304348217345719
1105	50-51	0.300657522837362
1105	52-53	0.35452040681122554
1105	54-55	0.47309218135222153
1105	56-57	0.27652485418424533
1105	58-59	0.299150700998716
1105	60-61	0.15337827063999754
1105	62-63	0.23970015048246296
1105	64-65	0.10131342658895903
1105	66-67	0.27866736570560136
1105	68-69	0.29589771269174747
1105	70-71	0.3877282493765435
1105	72-73	0.3785604160358531
1105	74-75	0.2307007686697844
1105	76-77	0.23781279670346578
1105	78-79	0.20305765247852747
1105	80-81	0.21214032078103884
1105	82-83	0.2625136088236708
1105	84-85	0.27546475619689303
1105	86-87	0.212999437994565
1105	88-89	0.2100762393059128
1105	90-91	0.22165342735569027
1105	92-93	0.2547598645535345
1105	94-95	0.2213813646193259
1105	96-97	0.19161556861471496
1105	98-99	0.2635566280036521
1105	100-101	0.2342759087045394
1106	1	0.17182221736392833
1106	2	0.19903274864915588
1106	3	0.2763076387045764
1106	4	0.1903218753821534
1106	5	0.26955465748044816
1106	6	0.2671760795404552
1106	7	0.24129474294906572
1106	8	0.3107673189054978
1106	9	0.37844607222134385
1106	10-11	0.3204422917585532
1106	12-13	0.3809036953302751
1106	14-15	0.3851363770720013
1106	16-17	0.37998859836103804
1106	18-19	0.3716531135858574
1106	20-21	0.4773785480310835
1106	22-23	0.39569212719152347
1106	24-25	0.25768549069174895
1106	26-27	0.32577277565630425
1106	28-29	0.4320561095034776
1106	30-31	0.3899964822343094
1106	32-33	0.3941567936831376
1106	34-35	0.4450189463105545
1106	36-37	0.5049384780073254
1106	38-39	0.4899671209556402
1106	40-41	0.4542938954959723
1106	42-43	0.3718777534947577
1106	44-45	0.40437701894185807
1106	46-47	0.3434101596627954
1106	48-49	0.3901191138663407
1106	50-51	0.37535739513457855
1106	52-53	0.4415829123430228
1106	54-55	0.35763313625258064
1106	56-57	0.2560579707728152
1106	58-59	0.3241254982888577
1106	60-61	0.28318824623076466
1106	62-63	0.32148089606775443
1106	64-65	0.3279395672787757
1106	66-67	0.34701661074884527
1106	68-69	0.2949005588360265
1106	70-71	0.35388534095230995
1106	72-73	0.315824354208047
1106	74-75	0.2673023304768023
1106	76-77	0.2978103207838174
1106	78-79	0.35512463337565237
1106	80-81	0.31478788738536423
1106	82-83	0.36124628502040323
1106	84-85	0.31206920205282174
1106	86-87	0.2451007201700044
1106	88-89	0.2873989238581416
1106	90-91	0.2477480596413919
1106	92-93	0.28170899933401117
1106	94-95	0.24909302739325412
1106	96-97	0.24873728391323624
1106	98-99	0.1331577247759519
1106	100-101	0.20934834680388192
1107	1	0.07699120145344551
1107	2	0.0907969373980464
1107	3	0.100299691676625
1107	4	0.06868189867630292
1107	5	0.10701086042463714
1107	6	0.05341112514104651
1107	7	0.1635181296526298
1107	8	-0.16063094393706479
1107	9	-0.09037542436911394
1107	10-11	-0.08409985896935979
1107	12-13	0.03116571090018283
1107	14-15	-0.19095577600829472
1107	16-17	-0.11627729275136289
1107	18-19	0.055010908295294314
1107	20-21	0.026754890747930915
1107	22-23	-0.025461034939787908
1107	24-25	-0.06306819781097062
1107	26-27	0.06678715066971819
1107	28-29	-0.12600003648934432
1107	30-31	-0.05721556773463732
1107	32-33	0.009627013914013105
1107	34-35	0.07307011959657217
1107	36-37	0.27558338764026047
1107	38-39	0.19454085319279102
1107	40-41	-0.1027507748966201
1107	42-43	-0.014393321578488383
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2216	66-67	-0.8243412908456875
2216	68-69	-0.9374328444011226
2216	70-71	-1.0878221857018602
2216	72-73	-0.9253064166603764
2216	74-75	-0.7427520643019889
2216	76-77	-0.752787067131024
2216	78-79	-0.814915099110479
2216	80-81	-0.8643063145582133
2216	82-83	-1.1494462924044342
2216	84-85	-0.8265999137301705
2216	86-87	-0.9649128512284584
2216	88-89	-0.7948136088129765
2216	90-91	-1.048109318247537
2216	92-93	-1.2643359346832845
2216	94-95	-0.9384474315068054
2216	96-97	-0.8132313548048948
2216	98-99	-0.8045021610936622
2216	100-101	-0.8611969739126408
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	55.0
11	983.0
12	4112.0
13	8319.0
14	12227.0
15	14467.0
16	16087.0
17	18364.0
18	23597.0
19	34020.0
20	32869.0
21	32622.0
22	37801.0
23	44753.0
24	51769.0
25	59303.0
26	68691.0
27	81704.0
28	95951.0
29	111559.0
30	129006.0
31	151914.0
32	186158.0
33	231778.0
34	301925.0
35	427101.0
36	692440.0
37	1078545.0
38	948883.0
39	159744.0
40	78.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.595752900949318	39.423459826889115	13.171105539374295	19.80968173278728
2	23.720813554547114	27.823504577362108	31.82080765899493	16.634874209095848
3	21.97624523377267	27.994690474193124	24.15187269800519	25.87719159402902
4	25.178666368023734	26.35356381998875	25.54471501925317	22.923054792734348
5	22.984639331730612	29.302585618850312	24.94901802826977	22.76375702114931
6	22.38776627085225	25.522622774693538	26.496576634165937	25.593034320288275
7	25.917035421860422	26.364763433620503	24.478949504603474	23.239251639915597
8	25.927710300086687	25.0823190913246	26.194192956464278	22.795777652124436
9	21.72254195343267	26.076052301530765	26.40642730266788	25.794978442368688
10-11	24.967860026534634	25.903869393747453	25.20201068385914	23.926259895858774
12-13	24.401420107016254	25.94952303847372	25.17627741802032	24.472779436489702
14-15	24.26108994195982	25.84553475049367	25.470126052169224	24.423249255377286
16-17	24.385750819091193	27.468921204297402	25.295096289686963	22.85023168692444
18-19	22.645905209322525	25.863411659127557	26.98137456884294	24.509308562706977
20-21	22.61936309884257	27.89676985040777	25.607440335754884	23.876426714994782
22-23	22.084990927650235	27.569955194024075	27.698910329108532	22.64614354921715
24-25	21.99793547274371	26.05883170859113	29.22234689556455	22.72088592310061
26-27	21.962576713445774	25.819603478183822	30.15181801299246	22.06600179537794
28-29	21.98916760153989	25.914745100024845	30.203795769889858	21.89229152854541
30-31	22.075499215661264	25.80550080263091	30.10649505641652	22.012504925291303
32-33	22.009278693238507	25.715515241575698	30.26496821635228	22.01023784883352
34-35	22.02406713239474	25.959559315367276	30.135153293670015	21.88122025856797
36-37	22.01479996689541	25.929467248455783	29.968415175073154	22.087317609575653
38-39	22.130163062288013	25.859048523144168	29.918983060903614	22.091805353664203
40-41	22.63850634084912	26.469445903444434	28.323200973908865	22.568846781797586
42-43	22.688835248666333	26.658105149250428	28.156235563810334	22.49682403827291
44-45	22.747105929181735	26.824740869473047	27.930121869560452	22.498031331784766
46-47	22.719379705768006	27.221185779380185	27.68900887631886	22.370425638532947
48-49	22.72947497105892	27.30501306550789	27.464322482126143	22.501189481307048
50-51	22.670639265464743	27.084380842256994	27.74850767985339	22.496472212424873
52-53	22.709203528430074	27.315323353893202	27.518806003089168	22.45666711458756
54-55	22.76742609273277	27.125720443891034	27.49146353895625	22.615389924419947
56-57	22.7383145079542	26.98858928505746	27.797426686225705	22.47566952076263
58-59	22.87016941345987	27.1223898064972	27.57995081075827	22.427489969284657
60-61	22.749200783258114	27.119105937202505	27.55486346573918	22.5768298138002
62-63	22.77533377656261	27.029563603730878	27.699371107489164	22.495731512217347
64-65	22.96512912748413	27.2183640506632	27.393081790545367	22.423425031307303
66-67	22.955565748633745	27.12090046518471	27.30652149473329	22.61701229144825
68-69	22.888082366290632	27.1854519020429	27.298091727894136	22.62837400377233
70-71	23.050188969123326	27.41060341084744	27.0107638687358	22.528443751293437
72-73	22.97839542031757	27.340652690771073	26.959839776375123	22.721112112536233
74-75	22.937846336517197	27.291740271133392	27.14053160544901	22.6298817869004
76-77	23.066353012275613	27.451525772516984	26.926245964909956	22.55587525029745
78-79	23.087882378288416	27.34062295851258	26.914228631562338	22.657266031636674
80-81	23.066447409598233	27.255037228778388	27.02648240028943	22.652032961333944
82-83	23.15672621488359	27.458829701684106	26.83398425932124	22.550459824111062
84-85	23.19653672086008	27.27405491112409	26.871559353634076	22.657849014381746
86-87	23.204684123993985	27.19603166096678	27.00878970326446	22.59049451177478
88-89	23.309532501217166	27.38761428611544	26.719191049879903	22.583662162787487
90-91	23.362886126594034	27.337662003794478	26.626672541234363	22.67277932837713
92-93	23.279830075229007	27.24177181496852	26.834767500404404	22.64363060939807
94-95	23.487786607646775	27.465835288776002	26.526253820083983	22.520124283493242
96-97	23.45258666206826	27.440928360991656	26.478743781178153	22.627741195761928
98-99	23.502212652758864	27.324669664330948	26.672264380858664	22.500853302051524
100-101	23.65953465671945	27.522985762215267	26.303287872669536	22.51419170839574
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2604.0
1	2064.0
2	1193.5
3	763.0
4	529.5
5	369.0
6	285.0
7	213.5
8	187.5
9	167.0
10	149.5
11	139.5
12	127.0
13	157.0
14	219.5
15	296.0
16	2413.0
17	2684.0
18	1018.5
19	1346.0
20	1924.0
21	2779.0
22	4101.0
23	5804.0
24	7615.5
25	10467.0
26	14786.0
27	21070.5
28	28140.5
29	36712.5
30	48517.0
31	60054.0
32	72950.5
33	93384.0
34	114606.0
35	133928.5
36	150680.5
37	160738.0
38	167301.0
39	175229.5
40	179308.5
41	175959.0
42	172219.5
43	174262.0
44	178853.0
45	184269.0
46	187517.5
47	189745.5
48	191958.5
49	187065.0
50	178394.5
51	172023.5
52	171981.0
53	170755.0
54	162483.5
55	152631.5
56	142099.5
57	127403.0
58	113635.0
59	103005.0
60	90759.5
61	76968.0
62	62434.0
63	48291.0
64	36388.5
65	25935.0
66	18444.5
67	13824.0
68	10347.5
69	7894.0
70	6143.0
71	4666.0
72	3662.5
73	2802.5
74	2017.5
75	1505.5
76	1126.5
77	730.5
78	450.5
79	234.0
80	91.5
81	41.5
82	20.5
83	9.5
84	4.0
85	3.0
86	2.5
87	2.0
88	2.0
89	1.5
90	1.0
91	1.5
92	1.0
93	0.0
94	1.5
95	2.5
96	1.5
97	1.0
98	1.0
99	2.5
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08586413691117709
2	0.042872742704613526
3	0.049714979317065686
4	0.0564187891772428
5	0.04148847518186309
6	0.01655188452203022
7	0.021317719850356725
8	0.011608071940778663
9	0.0029267370481009234
10-11	7.712347626752434E-4
12-13	4.746060078001497E-4
14-15	0.00139415514791294
16-17	3.955050065001248E-5
18-19	0.001661121027300524
20-21	0.005537070091001747
22-23	0.015899301261305017
24-25	0.018727162057780907
26-27	0.010065602415428176
28-29	0.017441770786655503
30-31	3.6584213101261543E-4
32-33	0.005833698845876841
34-35	2.47190629062578E-4
36-37	0.002481793915788283
38-39	0.009413019154702969
40-41	0.0017797725292505616
42-43	0.0017797725292505616
44-45	0.0021357270351006737
46-47	0.0
48-49	0.0
50-51	0.0036880841856136632
52-53	0.002560894917088308
54-55	0.0026597711687133393
56-57	0.0
58-59	0.004597745700563951
60-61	0.0
62-63	0.0
64-65	0.0019478621570131146
66-67	3.460668806876092E-4
68-69	0.0
70-71	9.393243904377963E-4
72-73	0.0028377484216383954
74-75	0.004696621952188982
76-77	0.0018292106550630773
78-79	8.898862646252808E-5
80-81	0.0010382006420628276
82-83	0.0010975263930378461
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	5056826.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.698984231002164
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.61380517935311	41.88436006051108
2	9.008653923288788	9.13459206398748
3	2.6755483154519712	4.069427455631517
4	1.2860883392073161	2.6081348971658995
5	0.7635235039550059	1.935493304350718
6	0.5400231141414058	1.642717400893909
7	0.4164188124746848	1.4778407565002685
8	0.31681669363763565	1.2849827643882186
9	0.2728780139924701	1.2451174315552287
>10	1.7338818853733302	17.10097119192595
>50	0.2659083885284598	9.502475697423996
>100	0.10594370724049561	7.533149204496667
>500	2.7468181631103996E-4	0.11357720148074972
>1k	1.9620128245771386E-4	0.20503180441232377
>5k	0.0	0.0
>10k+	3.924025649147964E-5	0.26212876527600515
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	13176	0.26055869828228223	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.955050065001248E-5	0.0	0.0	0.0	0.0
2	7.910100130002496E-5	0.0	0.0	0.0	0.0
3	1.1865150195003743E-4	0.0	0.0	0.0	0.0
4	1.1865150195003743E-4	0.0	0.0	0.0	0.0
5	1.7797725292505615E-4	0.0	0.0	0.0	0.0
6	4.94381258125156E-4	0.0	0.0	0.0	0.0
7	8.503357639752683E-4	0.0	0.0	0.0	0.0
8	0.001483143774375468	0.0	0.0	0.0	0.0
9	0.0037572975617511852	0.0	0.0	0.0	0.0
10-11	0.012972564213204092	3.955050065001248E-5	0.0	0.0	0.0
12-13	0.0228107512498947	3.955050065001248E-5	0.0	0.0	9.88762516250312E-6
14-15	0.03234242190654771	3.955050065001248E-5	0.0	0.0	1.977525032500624E-5
16-17	0.041379711305075555	3.955050065001248E-5	0.0	0.0	6.921337613752183E-5
18-19	0.047589139907127515	3.955050065001248E-5	0.0	0.0	7.910100130002496E-5
20-21	0.056280362424967756	3.955050065001248E-5	0.0	0.0	8.898862646252809E-5
22-23	0.0668700089740086	3.955050065001248E-5	0.0	0.0	9.88762516250312E-5
24-25	0.076055612749974	5.9325750975018714E-5	0.0	9.88762516250312E-6	9.88762516250312E-5
26-27	0.08932480571805318	5.9325750975018714E-5	0.0	1.977525032500624E-5	9.88762516250312E-5
28-29	0.09878726299856866	5.9325750975018714E-5	0.0	1.977525032500624E-5	9.88762516250312E-5
30-31	0.10760702464352145	5.9325750975018714E-5	0.0	1.977525032500624E-5	1.0876387678753432E-4
32-33	0.11775172806024964	5.9325750975018714E-5	0.0	1.977525032500624E-5	1.2853912711254054E-4
34-35	0.1284204756105905	7.910100130002496E-5	0.0	1.977525032500624E-5	1.3842675227504367E-4
36-37	0.13904967266028137	7.910100130002496E-5	0.0	2.966287548750936E-5	1.3842675227504367E-4
38-39	0.15078628372817257	7.910100130002496E-5	0.0	3.955050065001248E-5	1.3842675227504367E-4
40-41	0.16390716231881422	7.910100130002496E-5	0.0	4.9438125812515594E-5	1.3842675227504367E-4
42-43	0.17888691444000643	7.910100130002496E-5	0.0	5.9325750975018714E-5	1.3842675227504367E-4
44-45	0.1973866611190498	7.910100130002496E-5	0.0	6.921337613752183E-5	1.3842675227504367E-4
46-47	0.2180616853338438	7.910100130002496E-5	0.0	7.910100130002496E-5	1.483143774375468E-4
48-49	0.2412580539650761	7.910100130002496E-5	0.0	7.910100130002496E-5	1.5820200260004992E-4
50-51	0.26934879705174747	7.910100130002496E-5	0.0	7.910100130002496E-5	1.5820200260004992E-4
52-53	0.3016516684576452	7.910100130002496E-5	0.0	7.910100130002496E-5	1.5820200260004992E-4
54-55	0.3367131872838812	7.910100130002496E-5	0.0	7.910100130002496E-5	1.6808962776255305E-4
56-57	0.37654054143844384	7.910100130002496E-5	0.0	7.910100130002496E-5	1.977525032500624E-4
58-59	0.4229629415763959	7.910100130002496E-5	0.0	7.910100130002496E-5	1.977525032500624E-4
60-61	0.47557499506607503	7.910100130002496E-5	0.0	7.910100130002496E-5	2.076401284125655E-4
62-63	0.534159174153906	7.910100130002496E-5	0.0	8.898862646252809E-5	2.2741537873757176E-4
64-65	0.6010687336285647	7.910100130002496E-5	0.0	1.0876387678753432E-4	2.570782542250811E-4
66-67	0.6733274983161375	7.910100130002496E-5	0.0	1.1865150195003743E-4	3.1640400520009985E-4
68-69	0.7557606292959259	7.910100130002496E-5	0.0	1.2853912711254054E-4	3.559545058501123E-4
70-71	0.8471025105471297	7.910100130002496E-5	0.0	1.3842675227504367E-4	3.6584213101261543E-4
72-73	0.9461864023005735	7.910100130002496E-5	0.0	1.3842675227504367E-4	4.15280256825131E-4
74-75	1.0541098309492951	7.910100130002496E-5	0.0	1.3842675227504367E-4	4.449431323126404E-4
76-77	1.1749761609357332	7.910100130002496E-5	0.0	1.3842675227504367E-4	4.548307574751435E-4
78-79	1.3069660692299874	7.910100130002496E-5	0.0	1.3842675227504367E-4	4.548307574751435E-4
80-81	1.4507716895934326	7.910100130002496E-5	0.0	1.5820200260004992E-4	4.548307574751435E-4
82-83	1.6066105497796443	7.910100130002496E-5	0.0	1.7797725292505615E-4	4.844936329626529E-4
84-85	1.7773896115863983	7.910100130002496E-5	0.0	1.7797725292505615E-4	5.141565084501622E-4
86-87	1.9612104509824937	7.910100130002496E-5	0.0	1.8786487808755928E-4	5.339317587751684E-4
88-89	2.161949017031632	9.88762516250312E-5	0.0	1.977525032500624E-4	5.339317587751684E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACG	16940	0.0	90.028915	1
TATCTCG	6570	0.0	82.45639	2
TCGACGG	18545	0.0	82.357635	2
ATATCTC	6820	0.0	79.53574	1
CGACGGC	19405	0.0	78.6587	3
CGGCGCG	19975	0.0	76.1506	6
ATCTCGA	7230	0.0	74.7978	3
ACGGCGC	20435	0.0	74.500595	5
GACGGCG	20485	0.0	74.371025	4
TCTCGAG	5760	0.0	71.27997	4
TCGAGGG	6025	0.0	68.20211	6
AGGGCGC	6090	0.0	67.14689	9
CTCGAGG	6160	0.0	66.72327	5
CGAGGGC	6190	0.0	66.07323	7
CGCGCCG	25165	0.0	60.941216	9
CTCGACG	2155	0.0	59.974068	5
GCGCGCC	25865	0.0	59.31498	8
GGCGCGC	26025	0.0	58.900806	7
GAGGGCG	7325	0.0	56.02482	8
TCTCGAC	2650	0.0	48.775227	4
>>END_MODULE