FastQCFastQC Report
Tue 31 May 2016
SRR2048258_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048258_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5056826
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT238670.47197589950692387No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT142500.2817973171313389No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58930.11653555016526178No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT52820.10445287221668295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG45700.071.330321
TCGACGG53000.063.3884052
ATATCTC36650.063.272081
TATCTCG40950.056.396042
CGACGGC60150.055.6164553
ATCTCGA85750.047.103271
ACGGCGC72600.046.321035
GACGGCG72800.046.082884
CGGCGCG73950.045.4111866
TCTCGAG82500.044.005372
GGTCGAC5050.038.579691
TCGAGGG94650.038.3565034
CTCGAGG95550.037.9952163
CGAGGGC95900.037.8404545
GGGCGCG98600.036.7560848
AGGGCGC99900.036.2777757
GAGGGCG108350.033.536226
CTCGACG13700.029.135673
CGCGCCG169050.025.2876789
GGCGCGC175600.024.3985277
Produced by FastQC (version 0.11.5)